Amino acid dipepetide frequency for Moraxella phage Mcat13

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.768AlaAla: 6.768 ± 2.296
0.95AlaCys: 0.95 ± 0.476
4.631AlaAsp: 4.631 ± 0.548
6.412AlaGlu: 6.412 ± 1.087
4.275AlaPhe: 4.275 ± 1.244
6.056AlaGly: 6.056 ± 0.863
1.425AlaHis: 1.425 ± 0.381
6.531AlaIle: 6.531 ± 1.202
8.549AlaLys: 8.549 ± 1.205
9.024AlaLeu: 9.024 ± 1.036
2.85AlaMet: 2.85 ± 0.637
3.562AlaAsn: 3.562 ± 0.478
0.95AlaPro: 0.95 ± 0.304
4.512AlaGln: 4.512 ± 0.835
3.8AlaArg: 3.8 ± 0.623
5.818AlaSer: 5.818 ± 0.747
6.293AlaThr: 6.293 ± 0.957
5.937AlaVal: 5.937 ± 0.7
0.831AlaTrp: 0.831 ± 0.413
2.019AlaTyr: 2.019 ± 0.425
0.0AlaXaa: 0.0 ± 0.0
Cys
0.356CysAla: 0.356 ± 0.247
0.237CysCys: 0.237 ± 0.157
0.831CysAsp: 0.831 ± 0.308
0.237CysGlu: 0.237 ± 0.166
0.356CysPhe: 0.356 ± 0.18
0.594CysGly: 0.594 ± 0.376
0.594CysHis: 0.594 ± 0.259
0.237CysIle: 0.237 ± 0.167
0.712CysLys: 0.712 ± 0.474
0.475CysLeu: 0.475 ± 0.221
0.237CysMet: 0.237 ± 0.147
0.237CysAsn: 0.237 ± 0.177
0.237CysPro: 0.237 ± 0.162
0.594CysGln: 0.594 ± 0.293
1.069CysArg: 1.069 ± 0.345
0.831CysSer: 0.831 ± 0.422
0.831CysThr: 0.831 ± 0.411
0.475CysVal: 0.475 ± 0.239
0.0CysTrp: 0.0 ± 0.0
0.237CysTyr: 0.237 ± 0.162
0.0CysXaa: 0.0 ± 0.0
Asp
4.868AspAla: 4.868 ± 0.625
0.475AspCys: 0.475 ± 0.286
5.699AspAsp: 5.699 ± 0.933
4.987AspGlu: 4.987 ± 0.562
2.612AspPhe: 2.612 ± 0.54
6.531AspGly: 6.531 ± 0.849
0.594AspHis: 0.594 ± 0.306
5.581AspIle: 5.581 ± 0.8
3.8AspLys: 3.8 ± 0.501
3.918AspLeu: 3.918 ± 0.673
2.137AspMet: 2.137 ± 0.471
2.968AspAsn: 2.968 ± 0.449
1.544AspPro: 1.544 ± 0.512
0.594AspGln: 0.594 ± 0.259
2.731AspArg: 2.731 ± 0.445
2.493AspSer: 2.493 ± 0.517
2.375AspThr: 2.375 ± 0.532
3.562AspVal: 3.562 ± 0.523
0.95AspTrp: 0.95 ± 0.439
1.781AspTyr: 1.781 ± 0.413
0.0AspXaa: 0.0 ± 0.0
Glu
4.631GluAla: 4.631 ± 0.848
1.069GluCys: 1.069 ± 0.345
2.85GluAsp: 2.85 ± 0.617
2.85GluGlu: 2.85 ± 0.644
2.85GluPhe: 2.85 ± 0.605
1.9GluGly: 1.9 ± 0.362
1.544GluHis: 1.544 ± 0.291
4.749GluIle: 4.749 ± 0.681
5.106GluLys: 5.106 ± 0.843
6.293GluLeu: 6.293 ± 0.796
2.019GluMet: 2.019 ± 0.368
4.037GluAsn: 4.037 ± 0.607
2.375GluPro: 2.375 ± 0.641
4.393GluGln: 4.393 ± 0.778
3.206GluArg: 3.206 ± 0.59
1.781GluSer: 1.781 ± 0.377
4.156GluThr: 4.156 ± 0.571
3.8GluVal: 3.8 ± 0.681
0.594GluTrp: 0.594 ± 0.254
3.087GluTyr: 3.087 ± 0.48
0.0GluXaa: 0.0 ± 0.0
Phe
2.612PheAla: 2.612 ± 0.562
0.475PheCys: 0.475 ± 0.209
3.087PheAsp: 3.087 ± 0.707
3.087PheGlu: 3.087 ± 0.636
0.712PhePhe: 0.712 ± 0.264
4.156PheGly: 4.156 ± 0.765
0.712PheHis: 0.712 ± 0.304
2.375PheIle: 2.375 ± 0.567
1.544PheLys: 1.544 ± 0.45
2.256PheLeu: 2.256 ± 0.446
0.831PheMet: 0.831 ± 0.293
2.137PheAsn: 2.137 ± 0.534
1.187PhePro: 1.187 ± 0.28
0.475PheGln: 0.475 ± 0.279
1.306PheArg: 1.306 ± 0.458
2.137PheSer: 2.137 ± 0.609
1.662PheThr: 1.662 ± 0.409
1.662PheVal: 1.662 ± 0.567
0.831PheTrp: 0.831 ± 0.294
1.544PheTyr: 1.544 ± 0.404
0.0PheXaa: 0.0 ± 0.0
Gly
4.868GlyAla: 4.868 ± 0.596
0.356GlyCys: 0.356 ± 0.176
4.512GlyAsp: 4.512 ± 0.784
5.343GlyGlu: 5.343 ± 0.77
2.968GlyPhe: 2.968 ± 0.512
4.037GlyGly: 4.037 ± 0.883
0.831GlyHis: 0.831 ± 0.321
4.749GlyIle: 4.749 ± 0.835
6.531GlyLys: 6.531 ± 0.976
6.887GlyLeu: 6.887 ± 1.238
2.137GlyMet: 2.137 ± 0.405
3.443GlyAsn: 3.443 ± 0.555
0.237GlyPro: 0.237 ± 0.15
1.544GlyGln: 1.544 ± 0.42
3.325GlyArg: 3.325 ± 0.631
2.968GlySer: 2.968 ± 0.582
3.206GlyThr: 3.206 ± 0.801
5.581GlyVal: 5.581 ± 0.896
1.069GlyTrp: 1.069 ± 0.447
1.9GlyTyr: 1.9 ± 0.461
0.0GlyXaa: 0.0 ± 0.0
His
1.781HisAla: 1.781 ± 0.506
0.237HisCys: 0.237 ± 0.179
1.9HisAsp: 1.9 ± 0.473
0.831HisGlu: 0.831 ± 0.24
0.119HisPhe: 0.119 ± 0.121
2.019HisGly: 2.019 ± 0.491
1.069HisHis: 1.069 ± 0.467
1.9HisIle: 1.9 ± 0.409
1.306HisLys: 1.306 ± 0.368
1.781HisLeu: 1.781 ± 0.52
0.237HisMet: 0.237 ± 0.197
1.069HisAsn: 1.069 ± 0.323
1.187HisPro: 1.187 ± 0.52
0.831HisGln: 0.831 ± 0.347
0.594HisArg: 0.594 ± 0.253
0.475HisSer: 0.475 ± 0.24
1.425HisThr: 1.425 ± 0.384
0.594HisVal: 0.594 ± 0.308
0.594HisTrp: 0.594 ± 0.228
1.069HisTyr: 1.069 ± 0.324
0.0HisXaa: 0.0 ± 0.0
Ile
7.005IleAla: 7.005 ± 0.855
0.237IleCys: 0.237 ± 0.147
4.512IleAsp: 4.512 ± 0.691
3.562IleGlu: 3.562 ± 0.535
2.019IlePhe: 2.019 ± 0.478
4.512IleGly: 4.512 ± 0.71
1.187IleHis: 1.187 ± 0.289
4.393IleIle: 4.393 ± 0.589
6.412IleLys: 6.412 ± 1.119
4.749IleLeu: 4.749 ± 0.778
1.425IleMet: 1.425 ± 0.419
4.037IleAsn: 4.037 ± 0.847
2.256IlePro: 2.256 ± 0.477
2.493IleGln: 2.493 ± 0.649
1.9IleArg: 1.9 ± 0.381
4.275IleSer: 4.275 ± 0.99
4.631IleThr: 4.631 ± 0.762
3.681IleVal: 3.681 ± 0.604
0.475IleTrp: 0.475 ± 0.234
2.256IleTyr: 2.256 ± 0.584
0.0IleXaa: 0.0 ± 0.0
Lys
7.48LysAla: 7.48 ± 0.766
0.356LysCys: 0.356 ± 0.246
3.681LysAsp: 3.681 ± 0.65
4.037LysGlu: 4.037 ± 0.863
2.612LysPhe: 2.612 ± 0.545
4.631LysGly: 4.631 ± 0.712
1.306LysHis: 1.306 ± 0.354
4.987LysIle: 4.987 ± 0.712
6.649LysLys: 6.649 ± 1.28
6.887LysLeu: 6.887 ± 1.138
2.493LysMet: 2.493 ± 0.511
3.918LysAsn: 3.918 ± 0.75
2.612LysPro: 2.612 ± 0.551
4.156LysGln: 4.156 ± 0.668
4.156LysArg: 4.156 ± 0.976
5.699LysSer: 5.699 ± 0.97
4.037LysThr: 4.037 ± 0.877
4.037LysVal: 4.037 ± 0.677
0.712LysTrp: 0.712 ± 0.337
2.019LysTyr: 2.019 ± 0.576
0.0LysXaa: 0.0 ± 0.0
Leu
8.549LeuAla: 8.549 ± 1.119
0.712LeuCys: 0.712 ± 0.453
5.343LeuAsp: 5.343 ± 0.627
6.056LeuGlu: 6.056 ± 1.026
1.544LeuPhe: 1.544 ± 0.404
6.293LeuGly: 6.293 ± 0.879
1.425LeuHis: 1.425 ± 0.414
4.393LeuIle: 4.393 ± 0.66
8.549LeuLys: 8.549 ± 0.993
5.581LeuLeu: 5.581 ± 0.923
2.137LeuMet: 2.137 ± 0.646
3.8LeuAsn: 3.8 ± 0.765
4.275LeuPro: 4.275 ± 0.597
3.918LeuGln: 3.918 ± 0.525
4.037LeuArg: 4.037 ± 0.612
5.699LeuSer: 5.699 ± 0.626
4.868LeuThr: 4.868 ± 0.742
4.512LeuVal: 4.512 ± 0.668
1.069LeuTrp: 1.069 ± 0.3
3.325LeuTyr: 3.325 ± 0.423
0.0LeuXaa: 0.0 ± 0.0
Met
2.968MetAla: 2.968 ± 0.72
0.119MetCys: 0.119 ± 0.131
0.831MetAsp: 0.831 ± 0.355
0.594MetGlu: 0.594 ± 0.26
0.95MetPhe: 0.95 ± 0.343
2.019MetGly: 2.019 ± 0.384
0.712MetHis: 0.712 ± 0.324
1.781MetIle: 1.781 ± 0.378
1.425MetLys: 1.425 ± 0.347
1.544MetLeu: 1.544 ± 0.436
0.831MetMet: 0.831 ± 0.342
1.544MetAsn: 1.544 ± 0.369
1.069MetPro: 1.069 ± 0.372
1.544MetGln: 1.544 ± 0.377
1.069MetArg: 1.069 ± 0.381
2.731MetSer: 2.731 ± 0.531
1.9MetThr: 1.9 ± 0.59
2.968MetVal: 2.968 ± 0.853
0.237MetTrp: 0.237 ± 0.164
0.594MetTyr: 0.594 ± 0.298
0.0MetXaa: 0.0 ± 0.0
Asn
6.174AsnAla: 6.174 ± 1.084
0.0AsnCys: 0.0 ± 0.0
3.087AsnAsp: 3.087 ± 0.592
2.612AsnGlu: 2.612 ± 0.433
2.137AsnPhe: 2.137 ± 0.443
2.85AsnGly: 2.85 ± 0.535
0.95AsnHis: 0.95 ± 0.378
3.443AsnIle: 3.443 ± 0.611
3.681AsnLys: 3.681 ± 0.833
4.393AsnLeu: 4.393 ± 0.601
1.9AsnMet: 1.9 ± 0.558
3.087AsnAsn: 3.087 ± 0.724
2.968AsnPro: 2.968 ± 0.521
2.019AsnGln: 2.019 ± 0.448
2.256AsnArg: 2.256 ± 0.38
2.968AsnSer: 2.968 ± 0.468
2.375AsnThr: 2.375 ± 0.489
1.9AsnVal: 1.9 ± 0.454
0.594AsnTrp: 0.594 ± 0.201
1.544AsnTyr: 1.544 ± 0.338
0.0AsnXaa: 0.0 ± 0.0
Pro
2.256ProAla: 2.256 ± 0.422
0.831ProCys: 0.831 ± 0.333
1.662ProAsp: 1.662 ± 0.464
1.781ProGlu: 1.781 ± 0.35
1.069ProPhe: 1.069 ± 0.317
0.475ProGly: 0.475 ± 0.223
0.712ProHis: 0.712 ± 0.327
2.731ProIle: 2.731 ± 0.388
2.256ProLys: 2.256 ± 0.495
2.256ProLeu: 2.256 ± 0.526
1.187ProMet: 1.187 ± 0.42
3.443ProAsn: 3.443 ± 0.844
1.662ProPro: 1.662 ± 0.4
1.187ProGln: 1.187 ± 0.363
1.187ProArg: 1.187 ± 0.387
2.731ProSer: 2.731 ± 0.671
2.493ProThr: 2.493 ± 0.65
2.137ProVal: 2.137 ± 0.443
0.356ProTrp: 0.356 ± 0.204
0.95ProTyr: 0.95 ± 0.285
0.0ProXaa: 0.0 ± 0.0
Gln
5.818GlnAla: 5.818 ± 0.8
0.237GlnCys: 0.237 ± 0.154
2.493GlnAsp: 2.493 ± 0.745
2.256GlnGlu: 2.256 ± 0.582
0.712GlnPhe: 0.712 ± 0.256
2.731GlnGly: 2.731 ± 0.617
0.712GlnHis: 0.712 ± 0.292
3.325GlnIle: 3.325 ± 0.454
3.8GlnLys: 3.8 ± 0.522
4.868GlnLeu: 4.868 ± 0.743
1.187GlnMet: 1.187 ± 0.29
1.9GlnAsn: 1.9 ± 0.385
1.187GlnPro: 1.187 ± 0.386
1.9GlnGln: 1.9 ± 0.499
1.781GlnArg: 1.781 ± 0.489
2.731GlnSer: 2.731 ± 0.59
2.85GlnThr: 2.85 ± 0.551
2.137GlnVal: 2.137 ± 0.463
0.475GlnTrp: 0.475 ± 0.251
1.425GlnTyr: 1.425 ± 0.33
0.0GlnXaa: 0.0 ± 0.0
Arg
4.512ArgAla: 4.512 ± 0.878
0.356ArgCys: 0.356 ± 0.181
2.256ArgAsp: 2.256 ± 0.512
4.037ArgGlu: 4.037 ± 0.556
2.137ArgPhe: 2.137 ± 0.584
2.612ArgGly: 2.612 ± 0.517
1.425ArgHis: 1.425 ± 0.41
2.256ArgIle: 2.256 ± 0.606
1.781ArgLys: 1.781 ± 0.382
5.937ArgLeu: 5.937 ± 1.002
0.475ArgMet: 0.475 ± 0.198
1.781ArgAsn: 1.781 ± 0.402
1.306ArgPro: 1.306 ± 0.307
2.375ArgGln: 2.375 ± 0.519
2.137ArgArg: 2.137 ± 0.491
2.612ArgSer: 2.612 ± 0.453
2.612ArgThr: 2.612 ± 0.488
2.375ArgVal: 2.375 ± 0.39
0.712ArgTrp: 0.712 ± 0.265
2.612ArgTyr: 2.612 ± 0.533
0.0ArgXaa: 0.0 ± 0.0
Ser
5.699SerAla: 5.699 ± 0.699
0.475SerCys: 0.475 ± 0.23
3.087SerAsp: 3.087 ± 0.573
4.275SerGlu: 4.275 ± 0.659
2.493SerPhe: 2.493 ± 0.588
3.206SerGly: 3.206 ± 0.605
2.137SerHis: 2.137 ± 0.539
3.325SerIle: 3.325 ± 0.716
3.206SerLys: 3.206 ± 0.608
5.224SerLeu: 5.224 ± 0.774
1.187SerMet: 1.187 ± 0.381
3.087SerAsn: 3.087 ± 0.739
1.781SerPro: 1.781 ± 0.509
3.087SerGln: 3.087 ± 0.543
3.443SerArg: 3.443 ± 0.581
3.443SerSer: 3.443 ± 0.621
2.375SerThr: 2.375 ± 0.518
3.8SerVal: 3.8 ± 0.774
0.831SerTrp: 0.831 ± 0.261
2.137SerTyr: 2.137 ± 0.367
0.0SerXaa: 0.0 ± 0.0
Thr
6.768ThrAla: 6.768 ± 1.188
0.119ThrCys: 0.119 ± 0.113
4.393ThrAsp: 4.393 ± 0.636
4.156ThrGlu: 4.156 ± 0.663
1.425ThrPhe: 1.425 ± 0.414
4.393ThrGly: 4.393 ± 0.694
1.306ThrHis: 1.306 ± 0.442
3.325ThrIle: 3.325 ± 0.743
3.8ThrLys: 3.8 ± 0.572
4.393ThrLeu: 4.393 ± 0.751
1.544ThrMet: 1.544 ± 0.39
3.562ThrAsn: 3.562 ± 0.648
2.731ThrPro: 2.731 ± 0.705
2.256ThrGln: 2.256 ± 0.45
1.187ThrArg: 1.187 ± 0.437
2.375ThrSer: 2.375 ± 0.615
3.325ThrThr: 3.325 ± 0.76
3.8ThrVal: 3.8 ± 0.706
0.712ThrTrp: 0.712 ± 0.247
1.187ThrTyr: 1.187 ± 0.382
0.0ThrXaa: 0.0 ± 0.0
Val
4.512ValAla: 4.512 ± 0.795
1.069ValCys: 1.069 ± 0.456
2.612ValAsp: 2.612 ± 0.541
3.443ValGlu: 3.443 ± 0.614
2.137ValPhe: 2.137 ± 0.556
3.325ValGly: 3.325 ± 0.622
1.069ValHis: 1.069 ± 0.373
3.8ValIle: 3.8 ± 0.553
4.631ValLys: 4.631 ± 0.774
5.937ValLeu: 5.937 ± 0.896
1.544ValMet: 1.544 ± 0.406
2.968ValAsn: 2.968 ± 0.454
1.781ValPro: 1.781 ± 0.381
3.443ValGln: 3.443 ± 0.737
3.8ValArg: 3.8 ± 0.641
4.037ValSer: 4.037 ± 0.761
3.087ValThr: 3.087 ± 0.559
4.156ValVal: 4.156 ± 0.772
1.187ValTrp: 1.187 ± 0.332
1.9ValTyr: 1.9 ± 0.509
0.0ValXaa: 0.0 ± 0.0
Trp
1.306TrpAla: 1.306 ± 0.397
0.356TrpCys: 0.356 ± 0.191
0.594TrpAsp: 0.594 ± 0.354
0.831TrpGlu: 0.831 ± 0.269
0.356TrpPhe: 0.356 ± 0.225
0.594TrpGly: 0.594 ± 0.227
0.237TrpHis: 0.237 ± 0.138
0.594TrpIle: 0.594 ± 0.216
0.356TrpLys: 0.356 ± 0.223
1.306TrpLeu: 1.306 ± 0.35
0.237TrpMet: 0.237 ± 0.169
0.119TrpAsn: 0.119 ± 0.097
0.475TrpPro: 0.475 ± 0.257
1.662TrpGln: 1.662 ± 0.498
0.475TrpArg: 0.475 ± 0.222
0.831TrpSer: 0.831 ± 0.318
0.475TrpThr: 0.475 ± 0.215
0.95TrpVal: 0.95 ± 0.362
0.237TrpTrp: 0.237 ± 0.159
0.712TrpTyr: 0.712 ± 0.26
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.256TyrAla: 2.256 ± 0.476
0.712TyrCys: 0.712 ± 0.312
2.137TyrAsp: 2.137 ± 0.626
1.9TyrGlu: 1.9 ± 0.485
1.306TyrPhe: 1.306 ± 0.451
3.562TyrGly: 3.562 ± 0.647
1.069TyrHis: 1.069 ± 0.424
1.544TyrIle: 1.544 ± 0.416
2.493TyrLys: 2.493 ± 0.529
2.375TyrLeu: 2.375 ± 0.498
0.712TyrMet: 0.712 ± 0.307
0.237TyrAsn: 0.237 ± 0.19
1.544TyrPro: 1.544 ± 0.424
1.544TyrGln: 1.544 ± 0.337
2.85TyrArg: 2.85 ± 0.454
1.662TyrSer: 1.662 ± 0.431
1.781TyrThr: 1.781 ± 0.371
2.375TyrVal: 2.375 ± 0.592
0.237TyrTrp: 0.237 ± 0.15
2.019TyrTyr: 2.019 ± 0.519
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 44 proteins (8423 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski