Guangxi orbivirus

Taxonomy: Viruses; Riboviria; Orthornavirae; Duplornaviricota; Resentoviricetes; Reovirales; Reoviridae; Sedoreovirinae; Orbivirus; unclassified Orbivirus

Average proteome isoelectric point is 6.92

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A346RV23|A0A346RV23_9REOV NTPase OS=Guangxi orbivirus OX=2306813 GN=VP6 PE=4 SV=1
MM1 pKa = 7.34EE2 pKa = 6.05GIYY5 pKa = 10.76ARR7 pKa = 11.84TFCYY11 pKa = 9.82MEE13 pKa = 4.34CLGSNRR19 pKa = 11.84DD20 pKa = 3.13PRR22 pKa = 11.84ARR24 pKa = 11.84RR25 pKa = 11.84TYY27 pKa = 9.97IPSDD31 pKa = 3.57GFPLFVTRR39 pKa = 11.84FNSTTDD45 pKa = 3.25RR46 pKa = 11.84PITQTPSTIEE56 pKa = 3.55EE57 pKa = 4.22HH58 pKa = 6.83RR59 pKa = 11.84NCFYY63 pKa = 11.35AGLDD67 pKa = 3.69LLIAALGVNFNYY79 pKa = 10.45NLPDD83 pKa = 3.43YY84 pKa = 9.18TPNVQVLSLLARR96 pKa = 11.84EE97 pKa = 4.27DD98 pKa = 3.93LPYY101 pKa = 9.88STHH104 pKa = 7.13AYY106 pKa = 9.12IRR108 pKa = 11.84AQRR111 pKa = 11.84IVNEE115 pKa = 3.99PAAGQSTRR123 pKa = 11.84RR124 pKa = 11.84FYY126 pKa = 10.91TPWPDD131 pKa = 3.15VNFIYY136 pKa = 10.35PPATPYY142 pKa = 9.43RR143 pKa = 11.84TGADD147 pKa = 3.96EE148 pKa = 4.45PQPMCTGPQSMEE160 pKa = 3.62VTLNANEE167 pKa = 4.47DD168 pKa = 3.86LEE170 pKa = 4.45ITNTVMPNYY179 pKa = 9.17PDD181 pKa = 4.51VVDD184 pKa = 4.6TCITWYY190 pKa = 9.29TLSHH194 pKa = 6.02FRR196 pKa = 11.84GAGGALIEE204 pKa = 5.03GSQLCNLMIGEE215 pKa = 4.93DD216 pKa = 3.73EE217 pKa = 4.5VEE219 pKa = 4.24AGTEE223 pKa = 4.01IVVSADD229 pKa = 3.04AQIRR233 pKa = 11.84VANQSAVNAGMVHH246 pKa = 6.94IDD248 pKa = 3.33VRR250 pKa = 11.84WFTRR254 pKa = 11.84AQPSEE259 pKa = 4.81DD260 pKa = 3.4IYY262 pKa = 11.35DD263 pKa = 3.75TMRR266 pKa = 11.84VDD268 pKa = 3.18IASSYY273 pKa = 10.9SFHH276 pKa = 6.08SQQWYY281 pKa = 9.91DD282 pKa = 3.18LRR284 pKa = 11.84TYY286 pKa = 10.76LLRR289 pKa = 11.84VMGLPAHH296 pKa = 6.45MPTTEE301 pKa = 4.97PIRR304 pKa = 11.84NPQRR308 pKa = 11.84ILTLALISRR317 pKa = 11.84LLDD320 pKa = 3.02IYY322 pKa = 10.56VAHH325 pKa = 6.74SPIDD329 pKa = 3.41LTAIQGANGQAGIPDD344 pKa = 3.95AALDD348 pKa = 3.57ALRR351 pKa = 11.84RR352 pKa = 11.84VV353 pKa = 3.7

Molecular weight: 39.52 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A346RV24|A0A346RV24_9REOV Non-structural protein NS3 OS=Guangxi orbivirus OX=2306813 GN=NS3 PE=3 SV=1
MM1 pKa = 7.27HH2 pKa = 7.44AAISSAKK9 pKa = 10.04RR10 pKa = 11.84EE11 pKa = 4.02NDD13 pKa = 3.18PTSLVVVPATAPRR26 pKa = 11.84LEE28 pKa = 4.38EE29 pKa = 4.1VGEE32 pKa = 4.24VPLLDD37 pKa = 3.32KK38 pKa = 10.59RR39 pKa = 11.84VRR41 pKa = 11.84FALPDD46 pKa = 3.77VNVSTAKK53 pKa = 10.38SVALDD58 pKa = 3.41VLANALGGGTGTDD71 pKa = 3.15EE72 pKa = 4.02ITRR75 pKa = 11.84QEE77 pKa = 3.78RR78 pKa = 11.84SAYY81 pKa = 9.34GAAAQALNEE90 pKa = 4.19DD91 pKa = 4.35ANTKK95 pKa = 7.86VLRR98 pKa = 11.84VYY100 pKa = 10.34MNAQIMPRR108 pKa = 11.84LKK110 pKa = 10.84GKK112 pKa = 10.07LRR114 pKa = 11.84TANVKK119 pKa = 9.8YY120 pKa = 10.31RR121 pKa = 11.84FWLALEE127 pKa = 4.03ITLALISTAIFSIMAVTEE145 pKa = 3.95AEE147 pKa = 4.1KK148 pKa = 10.01TVNSTIEE155 pKa = 3.92RR156 pKa = 11.84VTGLKK161 pKa = 10.37VGLSAFSLICTGALLFASRR180 pKa = 11.84HH181 pKa = 5.18AGSLNEE187 pKa = 3.57FRR189 pKa = 11.84RR190 pKa = 11.84RR191 pKa = 11.84LRR193 pKa = 11.84RR194 pKa = 11.84EE195 pKa = 3.66VVKK198 pKa = 10.81RR199 pKa = 11.84EE200 pKa = 3.97TYY202 pKa = 10.65NFVANNHH209 pKa = 6.15EE210 pKa = 4.27IGRR213 pKa = 11.84LQIGTEE219 pKa = 3.99TRR221 pKa = 11.84GDD223 pKa = 3.48AGQSRR228 pKa = 11.84MNVVGNPAWISAPGNAAHH246 pKa = 7.35AEE248 pKa = 4.3GWQLSFVV255 pKa = 3.83

Molecular weight: 27.66 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10

0

10

6265

255

1316

626.5

71.06

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.496 ± 0.553

1.468 ± 0.258

5.443 ± 0.377

6.864 ± 0.408

3.847 ± 0.278

5.379 ± 0.297

2.203 ± 0.293

6.736 ± 0.357

5.698 ± 0.541

9.21 ± 0.315

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.384 ± 0.207

4.964 ± 0.286

3.911 ± 0.386

3.272 ± 0.193

6.672 ± 0.202

7.39 ± 0.427

5.666 ± 0.331

6.991 ± 0.578

1.038 ± 0.218

3.368 ± 0.293
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski