Pseudomonas phage vB_PaeS_SCH_Ab26

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Septimatrevirus; Pseudomonas virus Ab26

Average proteome isoelectric point is 6.55

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 52 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A060RFK4|A0A060RFK4_9CAUD Tail chaperonin OS=Pseudomonas phage vB_PaeS_SCH_Ab26 OX=1476390 GN=ORF17 PE=4 SV=1
MM1 pKa = 7.02ATKK4 pKa = 10.28KK5 pKa = 10.42VVYY8 pKa = 8.13FTAGINATSGEE19 pKa = 4.13LADD22 pKa = 3.75IAKK25 pKa = 10.19LNAAAVPQYY34 pKa = 10.65EE35 pKa = 4.21VLVANGAANAEE46 pKa = 4.2YY47 pKa = 10.98GEE49 pKa = 4.33TDD51 pKa = 4.27RR52 pKa = 11.84IIPTDD57 pKa = 4.19FVAGTVPSAYY67 pKa = 10.42SEE69 pKa = 4.23VDD71 pKa = 3.7VIDD74 pKa = 4.82PDD76 pKa = 5.09AIPNQALTPTQAIVNDD92 pKa = 4.06AEE94 pKa = 4.27ALTVPVTGTYY104 pKa = 7.23TTTATVSVANGVVTGIVLSS123 pKa = 3.99

Molecular weight: 12.52 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A060RJ55|A0A060RJ55_9CAUD Uncharacterized protein OS=Pseudomonas phage vB_PaeS_SCH_Ab26 OX=1476390 GN=ORF50 PE=4 SV=1
MM1 pKa = 7.68AWPLYY6 pKa = 10.24RR7 pKa = 11.84NEE9 pKa = 4.08EE10 pKa = 3.93ADD12 pKa = 3.76RR13 pKa = 11.84TSEE16 pKa = 3.74VGNRR20 pKa = 11.84KK21 pKa = 8.52GRR23 pKa = 11.84RR24 pKa = 11.84IGRR27 pKa = 11.84TTKK30 pKa = 10.08LRR32 pKa = 11.84RR33 pKa = 11.84IRR35 pKa = 11.84KK36 pKa = 8.78ARR38 pKa = 11.84RR39 pKa = 11.84LWLGRR44 pKa = 11.84LLWLGTRR51 pKa = 11.84RR52 pKa = 11.84NDD54 pKa = 3.1VAKK57 pKa = 9.96LYY59 pKa = 11.1RR60 pKa = 11.84MGFVMGLSCLACEE73 pKa = 4.52LTRR76 pKa = 11.84ARR78 pKa = 11.84LKK80 pKa = 10.69AAALALVGWPLDD92 pKa = 4.14RR93 pKa = 11.84IAGHH97 pKa = 7.19LSVCYY102 pKa = 9.93GEE104 pKa = 4.61RR105 pKa = 11.84YY106 pKa = 9.3YY107 pKa = 11.4VEE109 pKa = 4.14GGKK112 pKa = 10.28LYY114 pKa = 10.35RR115 pKa = 11.84ASKK118 pKa = 9.92LPPYY122 pKa = 9.67QPHH125 pKa = 7.56LIRR128 pKa = 11.84GEE130 pKa = 4.04EE131 pKa = 3.85

Molecular weight: 15.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

52

0

52

13451

67

1204

258.7

28.33

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.256 ± 0.795

1.137 ± 0.188

4.996 ± 0.247

6.215 ± 0.356

3.955 ± 0.174

7.91 ± 0.32

1.308 ± 0.191

4.892 ± 0.236

5.308 ± 0.366

8.051 ± 0.31

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.074 ± 0.117

4.899 ± 0.227

5.033 ± 0.438

4.104 ± 0.598

5.479 ± 0.286

5.933 ± 0.252

5.695 ± 0.3

7.048 ± 0.25

1.427 ± 0.139

3.264 ± 0.264
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski