Japanese macaque simian foamy virus

Taxonomy: Viruses; Riboviria; Pararnavirae; Artverviricota; Revtraviricetes; Ortervirales; Retroviridae; Spumaretrovirinae; Simiispumavirus

Average proteome isoelectric point is 6.95

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A077JM51|A0A077JM51_9RETR Bet protein OS=Japanese macaque simian foamy virus OX=2170198 GN=bet PE=4 SV=1
MM1 pKa = 7.61ASWEE5 pKa = 4.14DD6 pKa = 3.63QEE8 pKa = 4.3EE9 pKa = 4.23LRR11 pKa = 11.84EE12 pKa = 4.16LLHH15 pKa = 6.6HH16 pKa = 6.8LPEE19 pKa = 5.62DD20 pKa = 4.48DD21 pKa = 4.43PPADD25 pKa = 4.28LSHH28 pKa = 7.1LLNLDD33 pKa = 3.2EE34 pKa = 5.16MEE36 pKa = 4.49PKK38 pKa = 10.59VLGGQNPGDD47 pKa = 3.54EE48 pKa = 4.71KK49 pKa = 11.08LRR51 pKa = 11.84QQVIKK56 pKa = 9.64PPSIHH61 pKa = 6.98PSTVTWHH68 pKa = 6.54FGYY71 pKa = 10.18KK72 pKa = 10.11DD73 pKa = 3.91KK74 pKa = 11.11EE75 pKa = 4.2DD76 pKa = 3.58QQPEE80 pKa = 3.66IKK82 pKa = 9.91MRR84 pKa = 11.84DD85 pKa = 3.46WVPDD89 pKa = 3.82PSKK92 pKa = 10.33MSKK95 pKa = 7.51STCMRR100 pKa = 11.84LILSSKK106 pKa = 9.82EE107 pKa = 3.97LGSQSVALILAEE119 pKa = 4.08DD120 pKa = 3.97MKK122 pKa = 10.9HH123 pKa = 6.3SEE125 pKa = 5.48SILQLGNGAPRR136 pKa = 11.84KK137 pKa = 9.29GFPLTSSPLLPVVTPWPLSQDD158 pKa = 3.1HH159 pKa = 6.15VAPTLYY165 pKa = 10.66SLLVAYY171 pKa = 8.95YY172 pKa = 10.75KK173 pKa = 10.68SFQNQKK179 pKa = 10.51LEE181 pKa = 4.07PPKK184 pKa = 10.24WLWQCLEE191 pKa = 4.38DD192 pKa = 4.09PSGRR196 pKa = 11.84KK197 pKa = 9.64CMVTQFLVPPLGQVMISCYY216 pKa = 10.45RR217 pKa = 11.84NLTSIIICQAVDD229 pKa = 2.85PWEE232 pKa = 4.37NNNEE236 pKa = 4.03IDD238 pKa = 3.72WKK240 pKa = 9.15KK241 pKa = 11.25DD242 pKa = 2.8PMARR246 pKa = 11.84PRR248 pKa = 11.84IRR250 pKa = 11.84CDD252 pKa = 2.8HH253 pKa = 6.34ALCFKK258 pKa = 10.44VVYY261 pKa = 10.01EE262 pKa = 4.1GTPWRR267 pKa = 11.84PHH269 pKa = 6.0DD270 pKa = 3.74QKK272 pKa = 11.36CWFIRR277 pKa = 11.84LTEE280 pKa = 4.14GHH282 pKa = 5.5KK283 pKa = 10.26HH284 pKa = 5.16GMEE287 pKa = 4.15EE288 pKa = 5.02LSSGDD293 pKa = 3.0WKK295 pKa = 9.72TLQEE299 pKa = 4.25SRR301 pKa = 11.84PYY303 pKa = 9.98PYY305 pKa = 10.74GPVGEE310 pKa = 5.04DD311 pKa = 3.29PNLQYY316 pKa = 11.25AVSVKK321 pKa = 10.03MKK323 pKa = 9.59VTGGPLTSTVLALKK337 pKa = 9.78ALCFHH342 pKa = 7.25RR343 pKa = 11.84VNICNMDD350 pKa = 3.53NPGLGEE356 pKa = 3.8GHH358 pKa = 7.03PPLGYY363 pKa = 10.08SHH365 pKa = 7.23ALKK368 pKa = 10.41AYY370 pKa = 7.68GPQYY374 pKa = 10.2GSCEE378 pKa = 3.91EE379 pKa = 4.53RR380 pKa = 11.84VWQAATKK387 pKa = 10.41CIGPGEE393 pKa = 4.09GDD395 pKa = 3.42YY396 pKa = 10.4WCEE399 pKa = 3.59YY400 pKa = 9.35DD401 pKa = 3.32HH402 pKa = 7.31RR403 pKa = 11.84GYY405 pKa = 11.0FPIIPNKK412 pKa = 10.34LSPTWVRR419 pKa = 11.84HH420 pKa = 4.42AAPYY424 pKa = 9.86GIQRR428 pKa = 11.84LATPYY433 pKa = 10.32DD434 pKa = 3.41LQMFANEE441 pKa = 4.28LLPPGFSINTPSGACYY457 pKa = 9.23ISSRR461 pKa = 11.84RR462 pKa = 11.84LHH464 pKa = 6.19YY465 pKa = 10.94GNEE468 pKa = 4.16GTLQEE473 pKa = 4.2YY474 pKa = 9.94QEE476 pKa = 4.17NCDD479 pKa = 3.82RR480 pKa = 11.84IKK482 pKa = 10.67RR483 pKa = 11.84GYY485 pKa = 10.36EE486 pKa = 4.22DD487 pKa = 3.75ISSSDD492 pKa = 3.61SSDD495 pKa = 3.12EE496 pKa = 4.1DD497 pKa = 3.47

Molecular weight: 56.39 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A077JI63|A0A077JI63_9RETR Env leader protein OS=Japanese macaque simian foamy virus OX=2170198 GN=env PE=4 SV=1
MM1 pKa = 7.41AAIEE5 pKa = 4.57GDD7 pKa = 4.09LDD9 pKa = 3.89VQALTDD15 pKa = 4.0LFNNLGINRR24 pKa = 11.84DD25 pKa = 3.37PRR27 pKa = 11.84HH28 pKa = 6.25RR29 pKa = 11.84EE30 pKa = 3.84VIALRR35 pKa = 11.84MTGGWWGPATRR46 pKa = 11.84YY47 pKa = 10.39NLVTILLQDD56 pKa = 3.94DD57 pKa = 3.72QGQPLQQPRR66 pKa = 11.84WRR68 pKa = 11.84AEE70 pKa = 3.42GRR72 pKa = 11.84AANPAVMFTLEE83 pKa = 5.27APWQDD88 pKa = 3.25LRR90 pKa = 11.84MAFDD94 pKa = 5.12NIDD97 pKa = 3.69LADD100 pKa = 3.81DD101 pKa = 3.75TLRR104 pKa = 11.84FGPLANGNYY113 pKa = 9.57IPGDD117 pKa = 3.71EE118 pKa = 4.48YY119 pKa = 11.41SLEE122 pKa = 4.55FIPPAMQEE130 pKa = 3.74IAQMQRR136 pKa = 11.84DD137 pKa = 3.86EE138 pKa = 4.99LEE140 pKa = 4.03NVLDD144 pKa = 3.67IVGQITMQMSDD155 pKa = 4.6LIGMQDD161 pKa = 3.02AQIRR165 pKa = 11.84GLEE168 pKa = 4.03GQLRR172 pKa = 11.84GLRR175 pKa = 11.84GNLPVAGTPPPPPPSLDD192 pKa = 3.3IQPAAASSPYY202 pKa = 10.13VVPASSAPAAPAAPADD218 pKa = 4.28LGWFAGGPGPGSVDD232 pKa = 2.99PRR234 pKa = 11.84LARR237 pKa = 11.84VAYY240 pKa = 10.38NPFLPGPSDD249 pKa = 3.65GSGVAPVQPSAPPVASPLLPLPPAQPVQPIIQYY282 pKa = 7.09VHH284 pKa = 7.15PPPINPAQQVIPIQHH299 pKa = 5.77IRR301 pKa = 11.84AVTGNAPTNPRR312 pKa = 11.84EE313 pKa = 3.71IPMWIGRR320 pKa = 11.84NASAIEE326 pKa = 4.38GVFPMTTPDD335 pKa = 3.22LRR337 pKa = 11.84CRR339 pKa = 11.84VINALLGGNLGLNLEE354 pKa = 4.46PQHH357 pKa = 6.62CITWASAIATLYY369 pKa = 10.37VRR371 pKa = 11.84THH373 pKa = 5.85GSYY376 pKa = 10.45PIHH379 pKa = 6.36QLAEE383 pKa = 4.1VLRR386 pKa = 11.84GVANSEE392 pKa = 4.06GVAAAYY398 pKa = 9.55QLGMMLTNRR407 pKa = 11.84DD408 pKa = 3.54YY409 pKa = 11.76NLIWGIIRR417 pKa = 11.84PLLPGQAVVTAMQHH431 pKa = 6.74RR432 pKa = 11.84LDD434 pKa = 4.41QEE436 pKa = 4.08VSDD439 pKa = 4.28AARR442 pKa = 11.84IASFINHH449 pKa = 6.58LNGVYY454 pKa = 10.36EE455 pKa = 4.13LLGLNARR462 pKa = 11.84GQSLRR467 pKa = 11.84IPASGGQTTAGTSAGRR483 pKa = 11.84GARR486 pKa = 11.84GRR488 pKa = 11.84RR489 pKa = 11.84SQQGAPGRR497 pKa = 11.84QSSGQSQQQGRR508 pKa = 11.84RR509 pKa = 11.84STQGQSRR516 pKa = 11.84QSDD519 pKa = 3.32NSDD522 pKa = 3.1QNVQRR527 pKa = 11.84QSQGGNGRR535 pKa = 11.84GGYY538 pKa = 7.51NLRR541 pKa = 11.84PRR543 pKa = 11.84TYY545 pKa = 9.41QPQRR549 pKa = 11.84YY550 pKa = 8.66GGGRR554 pKa = 11.84GRR556 pKa = 11.84RR557 pKa = 11.84WNDD560 pKa = 2.85QPARR564 pKa = 11.84SDD566 pKa = 3.33NQQRR570 pKa = 11.84SQSQQPQSEE579 pKa = 4.15ARR581 pKa = 11.84GEE583 pKa = 3.9QSRR586 pKa = 11.84TSGAGRR592 pKa = 11.84GQGGRR597 pKa = 11.84GNQNRR602 pKa = 11.84NQRR605 pKa = 11.84LAGGNADD612 pKa = 3.5RR613 pKa = 11.84TVNTVTTASASTSASGQDD631 pKa = 3.74GSSPAPPASGSGNN644 pKa = 3.32

Molecular weight: 68.67 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

3579

308

1149

715.8

80.65

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.259 ± 0.999

1.565 ± 0.523

4.75 ± 0.348

5.644 ± 0.708

2.459 ± 0.284

6.007 ± 1.299

2.626 ± 0.317

5.979 ± 0.629

5.56 ± 1.3

9.835 ± 0.476

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.151 ± 0.261

4.554 ± 0.536

7.516 ± 0.992

6.147 ± 0.666

4.973 ± 0.781

7.097 ± 0.189

5.337 ± 0.512

5.7 ± 0.406

1.928 ± 0.178

3.912 ± 0.514
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski