Amino acid dipepetide frequency for Klebsiella phage KN1-1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.172AlaAla: 8.172 ± 1.248
0.591AlaCys: 0.591 ± 0.221
6.203AlaAsp: 6.203 ± 0.859
6.104AlaGlu: 6.104 ± 1.001
3.446AlaPhe: 3.446 ± 0.446
7.581AlaGly: 7.581 ± 1.569
1.378AlaHis: 1.378 ± 0.287
3.938AlaIle: 3.938 ± 0.584
5.612AlaLys: 5.612 ± 0.774
8.27AlaLeu: 8.27 ± 1.187
3.151AlaMet: 3.151 ± 0.618
4.726AlaAsn: 4.726 ± 0.5
2.757AlaPro: 2.757 ± 0.428
3.544AlaGln: 3.544 ± 0.674
5.021AlaArg: 5.021 ± 0.696
5.021AlaSer: 5.021 ± 0.726
3.741AlaThr: 3.741 ± 0.567
5.612AlaVal: 5.612 ± 0.782
1.181AlaTrp: 1.181 ± 0.386
3.249AlaTyr: 3.249 ± 0.62
0.0AlaXaa: 0.0 ± 0.0
Cys
0.492CysAla: 0.492 ± 0.21
0.197CysCys: 0.197 ± 0.158
0.886CysAsp: 0.886 ± 0.412
0.689CysGlu: 0.689 ± 0.261
0.394CysPhe: 0.394 ± 0.226
0.591CysGly: 0.591 ± 0.179
0.295CysHis: 0.295 ± 0.165
0.492CysIle: 0.492 ± 0.255
0.492CysLys: 0.492 ± 0.225
0.788CysLeu: 0.788 ± 0.345
0.0CysMet: 0.0 ± 0.0
0.295CysAsn: 0.295 ± 0.166
0.394CysPro: 0.394 ± 0.176
0.689CysGln: 0.689 ± 0.313
0.689CysArg: 0.689 ± 0.318
0.394CysSer: 0.394 ± 0.231
0.394CysThr: 0.394 ± 0.216
0.689CysVal: 0.689 ± 0.229
0.295CysTrp: 0.295 ± 0.194
0.197CysTyr: 0.197 ± 0.151
0.0CysXaa: 0.0 ± 0.0
Asp
5.218AspAla: 5.218 ± 0.7
0.591AspCys: 0.591 ± 0.343
4.135AspAsp: 4.135 ± 0.561
4.135AspGlu: 4.135 ± 0.586
2.757AspPhe: 2.757 ± 0.584
6.301AspGly: 6.301 ± 0.584
1.28AspHis: 1.28 ± 0.333
3.151AspIle: 3.151 ± 0.589
4.627AspLys: 4.627 ± 0.743
3.544AspLeu: 3.544 ± 0.67
2.068AspMet: 2.068 ± 0.509
1.871AspAsn: 1.871 ± 0.43
3.544AspPro: 3.544 ± 0.704
2.166AspGln: 2.166 ± 0.52
3.052AspArg: 3.052 ± 0.666
3.938AspSer: 3.938 ± 0.445
3.938AspThr: 3.938 ± 0.605
4.529AspVal: 4.529 ± 0.517
0.985AspTrp: 0.985 ± 0.381
2.363AspTyr: 2.363 ± 0.52
0.0AspXaa: 0.0 ± 0.0
Glu
6.892GluAla: 6.892 ± 1.064
0.492GluCys: 0.492 ± 0.23
4.529GluAsp: 4.529 ± 0.91
6.104GluGlu: 6.104 ± 1.152
2.954GluPhe: 2.954 ± 0.47
5.907GluGly: 5.907 ± 0.921
1.378GluHis: 1.378 ± 0.536
2.56GluIle: 2.56 ± 0.357
2.658GluLys: 2.658 ± 0.702
6.006GluLeu: 6.006 ± 1.021
1.575GluMet: 1.575 ± 0.675
2.264GluAsn: 2.264 ± 0.353
2.56GluPro: 2.56 ± 0.758
3.938GluGln: 3.938 ± 0.848
4.135GluArg: 4.135 ± 0.816
4.43GluSer: 4.43 ± 0.688
2.757GluThr: 2.757 ± 0.522
4.726GluVal: 4.726 ± 0.636
0.788GluTrp: 0.788 ± 0.277
2.757GluTyr: 2.757 ± 0.418
0.0GluXaa: 0.0 ± 0.0
Phe
2.757PheAla: 2.757 ± 0.459
0.492PheCys: 0.492 ± 0.232
2.56PheAsp: 2.56 ± 0.458
1.674PheGlu: 1.674 ± 0.346
0.689PhePhe: 0.689 ± 0.249
3.347PheGly: 3.347 ± 0.704
0.788PheHis: 0.788 ± 0.34
1.772PheIle: 1.772 ± 0.475
2.068PheLys: 2.068 ± 0.312
2.855PheLeu: 2.855 ± 0.564
1.181PheMet: 1.181 ± 0.279
2.068PheAsn: 2.068 ± 0.39
1.477PhePro: 1.477 ± 0.463
1.181PheGln: 1.181 ± 0.333
1.674PheArg: 1.674 ± 0.469
2.363PheSer: 2.363 ± 0.517
1.871PheThr: 1.871 ± 0.329
1.575PheVal: 1.575 ± 0.338
0.295PheTrp: 0.295 ± 0.157
0.985PheTyr: 0.985 ± 0.265
0.0PheXaa: 0.0 ± 0.0
Gly
6.99GlyAla: 6.99 ± 1.257
0.591GlyCys: 0.591 ± 0.242
6.006GlyAsp: 6.006 ± 0.544
5.809GlyGlu: 5.809 ± 0.821
2.855GlyPhe: 2.855 ± 0.358
5.612GlyGly: 5.612 ± 0.861
1.575GlyHis: 1.575 ± 0.556
5.12GlyIle: 5.12 ± 1.025
5.907GlyLys: 5.907 ± 0.834
6.006GlyLeu: 6.006 ± 0.769
1.871GlyMet: 1.871 ± 0.4
3.151GlyAsn: 3.151 ± 0.44
1.28GlyPro: 1.28 ± 0.359
3.249GlyGln: 3.249 ± 0.447
4.332GlyArg: 4.332 ± 0.416
5.612GlySer: 5.612 ± 0.723
4.824GlyThr: 4.824 ± 0.613
4.824GlyVal: 4.824 ± 0.729
1.575GlyTrp: 1.575 ± 0.43
3.249GlyTyr: 3.249 ± 0.644
0.0GlyXaa: 0.0 ± 0.0
His
1.674HisAla: 1.674 ± 0.446
0.394HisCys: 0.394 ± 0.176
1.083HisAsp: 1.083 ± 0.246
1.28HisGlu: 1.28 ± 0.373
0.591HisPhe: 0.591 ± 0.231
1.575HisGly: 1.575 ± 0.474
0.394HisHis: 0.394 ± 0.222
0.591HisIle: 0.591 ± 0.191
0.788HisLys: 0.788 ± 0.244
1.772HisLeu: 1.772 ± 0.599
0.492HisMet: 0.492 ± 0.178
0.295HisAsn: 0.295 ± 0.158
0.985HisPro: 0.985 ± 0.298
0.689HisGln: 0.689 ± 0.246
0.985HisArg: 0.985 ± 0.306
0.985HisSer: 0.985 ± 0.292
0.788HisThr: 0.788 ± 0.238
1.575HisVal: 1.575 ± 0.345
0.197HisTrp: 0.197 ± 0.138
0.985HisTyr: 0.985 ± 0.21
0.0HisXaa: 0.0 ± 0.0
Ile
4.726IleAla: 4.726 ± 0.521
0.689IleCys: 0.689 ± 0.24
3.741IleAsp: 3.741 ± 0.526
3.151IleGlu: 3.151 ± 0.507
0.788IlePhe: 0.788 ± 0.271
3.741IleGly: 3.741 ± 0.586
1.083IleHis: 1.083 ± 0.391
2.757IleIle: 2.757 ± 0.562
3.741IleLys: 3.741 ± 0.53
3.446IleLeu: 3.446 ± 0.453
1.083IleMet: 1.083 ± 0.338
2.068IleAsn: 2.068 ± 0.634
2.264IlePro: 2.264 ± 0.389
1.772IleGln: 1.772 ± 0.411
3.249IleArg: 3.249 ± 0.606
3.249IleSer: 3.249 ± 0.507
2.461IleThr: 2.461 ± 0.606
2.461IleVal: 2.461 ± 0.483
0.591IleTrp: 0.591 ± 0.253
1.772IleTyr: 1.772 ± 0.439
0.0IleXaa: 0.0 ± 0.0
Lys
7.286LysAla: 7.286 ± 1.022
0.492LysCys: 0.492 ± 0.269
3.544LysAsp: 3.544 ± 0.725
4.726LysGlu: 4.726 ± 0.688
1.871LysPhe: 1.871 ± 0.415
5.612LysGly: 5.612 ± 0.873
1.772LysHis: 1.772 ± 0.378
2.166LysIle: 2.166 ± 0.55
3.249LysLys: 3.249 ± 0.793
5.71LysLeu: 5.71 ± 0.737
1.674LysMet: 1.674 ± 0.448
2.757LysAsn: 2.757 ± 0.37
2.166LysPro: 2.166 ± 0.689
2.264LysGln: 2.264 ± 0.379
3.052LysArg: 3.052 ± 0.632
2.658LysSer: 2.658 ± 0.558
3.347LysThr: 3.347 ± 0.564
4.923LysVal: 4.923 ± 0.726
0.788LysTrp: 0.788 ± 0.343
1.772LysTyr: 1.772 ± 0.401
0.0LysXaa: 0.0 ± 0.0
Leu
7.975LeuAla: 7.975 ± 1.281
0.295LeuCys: 0.295 ± 0.155
4.332LeuAsp: 4.332 ± 0.691
6.596LeuGlu: 6.596 ± 1.075
2.461LeuPhe: 2.461 ± 0.48
5.218LeuGly: 5.218 ± 0.71
1.28LeuHis: 1.28 ± 0.339
3.544LeuIle: 3.544 ± 0.61
5.71LeuLys: 5.71 ± 0.528
6.203LeuLeu: 6.203 ± 0.996
2.068LeuMet: 2.068 ± 0.33
4.332LeuAsn: 4.332 ± 0.545
3.84LeuPro: 3.84 ± 0.653
3.741LeuGln: 3.741 ± 0.604
5.809LeuArg: 5.809 ± 0.672
6.006LeuSer: 6.006 ± 0.759
5.021LeuThr: 5.021 ± 0.817
5.021LeuVal: 5.021 ± 0.882
1.083LeuTrp: 1.083 ± 0.401
2.068LeuTyr: 2.068 ± 0.473
0.0LeuXaa: 0.0 ± 0.0
Met
3.151MetAla: 3.151 ± 0.506
0.197MetCys: 0.197 ± 0.129
2.068MetAsp: 2.068 ± 0.365
1.181MetGlu: 1.181 ± 0.358
0.689MetPhe: 0.689 ± 0.27
1.477MetGly: 1.477 ± 0.354
0.492MetHis: 0.492 ± 0.229
1.083MetIle: 1.083 ± 0.266
1.378MetLys: 1.378 ± 0.391
2.855MetLeu: 2.855 ± 0.535
1.181MetMet: 1.181 ± 0.338
0.985MetAsn: 0.985 ± 0.243
1.181MetPro: 1.181 ± 0.414
2.264MetGln: 2.264 ± 0.551
1.477MetArg: 1.477 ± 0.363
0.985MetSer: 0.985 ± 0.335
2.166MetThr: 2.166 ± 0.515
1.575MetVal: 1.575 ± 0.457
0.098MetTrp: 0.098 ± 0.095
0.492MetTyr: 0.492 ± 0.228
0.0MetXaa: 0.0 ± 0.0
Asn
4.037AsnAla: 4.037 ± 0.759
0.591AsnCys: 0.591 ± 0.265
1.575AsnAsp: 1.575 ± 0.381
2.658AsnGlu: 2.658 ± 0.648
1.477AsnPhe: 1.477 ± 0.322
4.135AsnGly: 4.135 ± 0.658
0.492AsnHis: 0.492 ± 0.198
2.757AsnIle: 2.757 ± 0.481
1.575AsnLys: 1.575 ± 0.378
3.249AsnLeu: 3.249 ± 0.588
0.985AsnMet: 0.985 ± 0.291
1.477AsnAsn: 1.477 ± 0.382
2.461AsnPro: 2.461 ± 0.318
1.674AsnGln: 1.674 ± 0.338
1.871AsnArg: 1.871 ± 0.555
3.052AsnSer: 3.052 ± 0.546
1.871AsnThr: 1.871 ± 0.32
2.855AsnVal: 2.855 ± 0.569
0.788AsnTrp: 0.788 ± 0.298
1.969AsnTyr: 1.969 ± 0.441
0.0AsnXaa: 0.0 ± 0.0
Pro
2.855ProAla: 2.855 ± 0.519
0.788ProCys: 0.788 ± 0.239
1.969ProAsp: 1.969 ± 0.479
4.726ProGlu: 4.726 ± 0.765
1.575ProPhe: 1.575 ± 0.307
2.855ProGly: 2.855 ± 0.491
0.295ProHis: 0.295 ± 0.13
1.181ProIle: 1.181 ± 0.338
2.166ProLys: 2.166 ± 0.492
3.446ProLeu: 3.446 ± 0.685
0.886ProMet: 0.886 ± 0.342
2.166ProAsn: 2.166 ± 0.504
1.181ProPro: 1.181 ± 0.427
1.378ProGln: 1.378 ± 0.327
1.871ProArg: 1.871 ± 0.487
2.068ProSer: 2.068 ± 0.337
2.068ProThr: 2.068 ± 0.495
3.544ProVal: 3.544 ± 0.583
0.886ProTrp: 0.886 ± 0.249
2.068ProTyr: 2.068 ± 0.461
0.0ProXaa: 0.0 ± 0.0
Gln
3.84GlnAla: 3.84 ± 0.732
0.098GlnCys: 0.098 ± 0.105
2.658GlnAsp: 2.658 ± 0.414
2.954GlnGlu: 2.954 ± 0.458
1.772GlnPhe: 1.772 ± 0.374
3.249GlnGly: 3.249 ± 0.526
0.492GlnHis: 0.492 ± 0.233
1.969GlnIle: 1.969 ± 0.443
3.544GlnLys: 3.544 ± 0.548
4.529GlnLeu: 4.529 ± 0.474
1.28GlnMet: 1.28 ± 0.386
1.378GlnAsn: 1.378 ± 0.352
1.969GlnPro: 1.969 ± 0.327
3.643GlnGln: 3.643 ± 0.686
2.363GlnArg: 2.363 ± 0.71
2.56GlnSer: 2.56 ± 0.537
1.575GlnThr: 1.575 ± 0.45
2.855GlnVal: 2.855 ± 0.402
0.788GlnTrp: 0.788 ± 0.278
1.575GlnTyr: 1.575 ± 0.571
0.0GlnXaa: 0.0 ± 0.0
Arg
5.218ArgAla: 5.218 ± 0.753
0.886ArgCys: 0.886 ± 0.271
3.741ArgAsp: 3.741 ± 0.581
3.544ArgGlu: 3.544 ± 0.526
1.477ArgPhe: 1.477 ± 0.36
4.037ArgGly: 4.037 ± 0.737
0.689ArgHis: 0.689 ± 0.263
2.855ArgIle: 2.855 ± 0.431
4.037ArgLys: 4.037 ± 0.682
4.135ArgLeu: 4.135 ± 0.628
1.28ArgMet: 1.28 ± 0.315
2.166ArgAsn: 2.166 ± 0.388
2.363ArgPro: 2.363 ± 0.432
2.954ArgGln: 2.954 ± 0.523
3.249ArgArg: 3.249 ± 0.452
3.741ArgSer: 3.741 ± 0.464
3.643ArgThr: 3.643 ± 0.715
3.151ArgVal: 3.151 ± 0.381
0.689ArgTrp: 0.689 ± 0.328
1.772ArgTyr: 1.772 ± 0.519
0.0ArgXaa: 0.0 ± 0.0
Ser
4.726SerAla: 4.726 ± 0.675
0.492SerCys: 0.492 ± 0.197
4.037SerAsp: 4.037 ± 0.586
3.84SerGlu: 3.84 ± 0.547
3.052SerPhe: 3.052 ± 0.755
4.923SerGly: 4.923 ± 0.636
1.575SerHis: 1.575 ± 0.444
3.151SerIle: 3.151 ± 0.483
3.84SerLys: 3.84 ± 0.533
4.234SerLeu: 4.234 ± 1.048
1.772SerMet: 1.772 ± 0.332
1.28SerAsn: 1.28 ± 0.416
2.461SerPro: 2.461 ± 0.548
2.954SerGln: 2.954 ± 0.553
3.643SerArg: 3.643 ± 0.592
3.643SerSer: 3.643 ± 0.9
4.234SerThr: 4.234 ± 0.854
3.643SerVal: 3.643 ± 0.586
1.083SerTrp: 1.083 ± 0.263
2.658SerTyr: 2.658 ± 0.531
0.0SerXaa: 0.0 ± 0.0
Thr
4.43ThrAla: 4.43 ± 0.714
0.591ThrCys: 0.591 ± 0.281
3.84ThrAsp: 3.84 ± 0.505
2.855ThrGlu: 2.855 ± 0.47
1.772ThrPhe: 1.772 ± 0.429
5.612ThrGly: 5.612 ± 0.625
1.181ThrHis: 1.181 ± 0.266
3.544ThrIle: 3.544 ± 0.694
3.446ThrLys: 3.446 ± 0.458
5.809ThrLeu: 5.809 ± 0.765
1.378ThrMet: 1.378 ± 0.376
1.969ThrAsn: 1.969 ± 0.57
2.757ThrPro: 2.757 ± 0.481
2.264ThrGln: 2.264 ± 0.417
2.166ThrArg: 2.166 ± 0.389
3.741ThrSer: 3.741 ± 0.621
2.954ThrThr: 2.954 ± 0.748
2.855ThrVal: 2.855 ± 0.725
0.689ThrTrp: 0.689 ± 0.273
1.871ThrTyr: 1.871 ± 0.509
0.0ThrXaa: 0.0 ± 0.0
Val
5.71ValAla: 5.71 ± 0.799
0.394ValCys: 0.394 ± 0.211
3.347ValAsp: 3.347 ± 0.494
4.135ValGlu: 4.135 ± 0.602
1.772ValPhe: 1.772 ± 0.628
4.824ValGly: 4.824 ± 0.461
0.886ValHis: 0.886 ± 0.296
3.84ValIle: 3.84 ± 0.702
3.84ValLys: 3.84 ± 0.515
5.907ValLeu: 5.907 ± 0.978
1.181ValMet: 1.181 ± 0.319
3.84ValAsn: 3.84 ± 0.917
2.757ValPro: 2.757 ± 0.612
2.461ValGln: 2.461 ± 0.382
3.84ValArg: 3.84 ± 0.491
4.234ValSer: 4.234 ± 0.966
4.923ValThr: 4.923 ± 0.599
4.824ValVal: 4.824 ± 0.892
0.788ValTrp: 0.788 ± 0.337
2.56ValTyr: 2.56 ± 0.539
0.0ValXaa: 0.0 ± 0.0
Trp
0.591TrpAla: 0.591 ± 0.234
0.492TrpCys: 0.492 ± 0.27
0.689TrpAsp: 0.689 ± 0.279
0.886TrpGlu: 0.886 ± 0.228
0.394TrpPhe: 0.394 ± 0.197
0.689TrpGly: 0.689 ± 0.3
0.295TrpHis: 0.295 ± 0.242
0.788TrpIle: 0.788 ± 0.362
1.28TrpLys: 1.28 ± 0.439
1.575TrpLeu: 1.575 ± 0.441
0.295TrpMet: 0.295 ± 0.15
0.788TrpAsn: 0.788 ± 0.253
0.197TrpPro: 0.197 ± 0.135
0.689TrpGln: 0.689 ± 0.219
0.788TrpArg: 0.788 ± 0.239
0.985TrpSer: 0.985 ± 0.385
0.886TrpThr: 0.886 ± 0.29
1.477TrpVal: 1.477 ± 0.361
0.295TrpTrp: 0.295 ± 0.151
0.295TrpTyr: 0.295 ± 0.201
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.56TyrAla: 2.56 ± 0.497
0.0TyrCys: 0.0 ± 0.0
3.249TyrAsp: 3.249 ± 0.434
2.363TyrGlu: 2.363 ± 0.576
0.985TyrPhe: 0.985 ± 0.228
3.151TyrGly: 3.151 ± 0.465
0.492TyrHis: 0.492 ± 0.308
1.575TyrIle: 1.575 ± 0.511
1.871TyrLys: 1.871 ± 0.309
2.264TyrLeu: 2.264 ± 0.425
1.477TyrMet: 1.477 ± 0.319
1.674TyrAsn: 1.674 ± 0.452
1.378TyrPro: 1.378 ± 0.356
1.575TyrGln: 1.575 ± 0.523
2.363TyrArg: 2.363 ± 0.425
1.575TyrSer: 1.575 ± 0.351
2.264TyrThr: 2.264 ± 0.557
3.347TyrVal: 3.347 ± 0.71
0.492TyrTrp: 0.492 ± 0.222
0.985TyrTyr: 0.985 ± 0.4
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 22 proteins (10158 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski