Amino acid dipepetide frequency for Streptomyces phage Araceli

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
15.406AlaAla: 15.406 ± 1.231
0.885AlaCys: 0.885 ± 0.282
7.615AlaAsp: 7.615 ± 0.789
8.559AlaGlu: 8.559 ± 1.087
3.188AlaPhe: 3.188 ± 0.602
9.799AlaGly: 9.799 ± 0.868
1.889AlaHis: 1.889 ± 0.385
4.132AlaIle: 4.132 ± 0.703
8.677AlaLys: 8.677 ± 1.66
9.208AlaLeu: 9.208 ± 0.823
3.424AlaMet: 3.424 ± 0.407
2.833AlaAsn: 2.833 ± 0.488
4.604AlaPro: 4.604 ± 0.681
4.309AlaGln: 4.309 ± 0.801
8.146AlaArg: 8.146 ± 0.608
4.781AlaSer: 4.781 ± 0.58
6.375AlaThr: 6.375 ± 0.654
7.556AlaVal: 7.556 ± 0.723
2.243AlaTrp: 2.243 ± 0.39
3.306AlaTyr: 3.306 ± 0.464
0.0AlaXaa: 0.0 ± 0.0
Cys
0.885CysAla: 0.885 ± 0.247
0.0CysCys: 0.0 ± 0.0
0.767CysAsp: 0.767 ± 0.22
0.826CysGlu: 0.826 ± 0.31
0.118CysPhe: 0.118 ± 0.083
1.181CysGly: 1.181 ± 0.363
0.295CysHis: 0.295 ± 0.123
0.59CysIle: 0.59 ± 0.215
0.472CysLys: 0.472 ± 0.16
0.59CysLeu: 0.59 ± 0.178
0.177CysMet: 0.177 ± 0.101
0.236CysAsn: 0.236 ± 0.119
0.885CysPro: 0.885 ± 0.317
0.472CysGln: 0.472 ± 0.203
0.649CysArg: 0.649 ± 0.255
0.767CysSer: 0.767 ± 0.279
0.708CysThr: 0.708 ± 0.21
0.708CysVal: 0.708 ± 0.188
0.118CysTrp: 0.118 ± 0.081
0.236CysTyr: 0.236 ± 0.119
0.0CysXaa: 0.0 ± 0.0
Asp
7.379AspAla: 7.379 ± 0.679
0.59AspCys: 0.59 ± 0.217
4.073AspAsp: 4.073 ± 0.576
4.899AspGlu: 4.899 ± 0.79
2.479AspPhe: 2.479 ± 0.385
6.139AspGly: 6.139 ± 0.655
1.003AspHis: 1.003 ± 0.271
2.184AspIle: 2.184 ± 0.346
2.715AspLys: 2.715 ± 0.414
5.076AspLeu: 5.076 ± 0.727
1.653AspMet: 1.653 ± 0.297
1.594AspAsn: 1.594 ± 0.316
3.365AspPro: 3.365 ± 0.544
1.712AspGln: 1.712 ± 0.273
4.014AspArg: 4.014 ± 0.459
2.184AspSer: 2.184 ± 0.355
2.892AspThr: 2.892 ± 0.451
4.309AspVal: 4.309 ± 0.527
1.476AspTrp: 1.476 ± 0.276
1.535AspTyr: 1.535 ± 0.289
0.0AspXaa: 0.0 ± 0.0
Glu
7.024GluAla: 7.024 ± 0.723
1.122GluCys: 1.122 ± 0.308
4.132GluAsp: 4.132 ± 0.731
3.424GluGlu: 3.424 ± 0.608
2.184GluPhe: 2.184 ± 0.333
4.781GluGly: 4.781 ± 0.649
1.535GluHis: 1.535 ± 0.312
2.833GluIle: 2.833 ± 0.485
3.542GluLys: 3.542 ± 0.722
5.135GluLeu: 5.135 ± 0.698
1.358GluMet: 1.358 ± 0.254
1.712GluAsn: 1.712 ± 0.346
3.483GluPro: 3.483 ± 0.573
3.069GluGln: 3.069 ± 0.483
5.194GluArg: 5.194 ± 0.729
2.774GluSer: 2.774 ± 0.41
3.601GluThr: 3.601 ± 0.551
3.66GluVal: 3.66 ± 0.471
1.535GluTrp: 1.535 ± 0.335
0.944GluTyr: 0.944 ± 0.284
0.0GluXaa: 0.0 ± 0.0
Phe
2.302PheAla: 2.302 ± 0.339
0.295PheCys: 0.295 ± 0.14
2.066PheAsp: 2.066 ± 0.313
1.653PheGlu: 1.653 ± 0.481
0.944PhePhe: 0.944 ± 0.186
2.597PheGly: 2.597 ± 0.353
0.767PheHis: 0.767 ± 0.195
0.826PheIle: 0.826 ± 0.238
1.181PheLys: 1.181 ± 0.329
2.361PheLeu: 2.361 ± 0.363
0.59PheMet: 0.59 ± 0.202
1.122PheAsn: 1.122 ± 0.282
1.476PhePro: 1.476 ± 0.306
1.358PheGln: 1.358 ± 0.271
2.42PheArg: 2.42 ± 0.419
1.594PheSer: 1.594 ± 0.27
2.184PheThr: 2.184 ± 0.329
2.774PheVal: 2.774 ± 0.411
0.708PheTrp: 0.708 ± 0.167
0.649PheTyr: 0.649 ± 0.179
0.0PheXaa: 0.0 ± 0.0
Gly
9.445GlyAla: 9.445 ± 0.718
0.944GlyCys: 0.944 ± 0.243
5.549GlyAsp: 5.549 ± 0.669
5.431GlyGlu: 5.431 ± 0.543
3.01GlyPhe: 3.01 ± 0.286
9.326GlyGly: 9.326 ± 1.04
1.948GlyHis: 1.948 ± 0.346
3.01GlyIle: 3.01 ± 0.718
5.372GlyLys: 5.372 ± 0.756
6.08GlyLeu: 6.08 ± 0.483
2.479GlyMet: 2.479 ± 0.361
3.01GlyAsn: 3.01 ± 0.499
3.719GlyPro: 3.719 ± 0.439
3.483GlyGln: 3.483 ± 0.429
4.722GlyArg: 4.722 ± 0.372
5.135GlySer: 5.135 ± 0.631
5.608GlyThr: 5.608 ± 0.684
7.733GlyVal: 7.733 ± 0.792
2.715GlyTrp: 2.715 ± 0.457
3.188GlyTyr: 3.188 ± 0.461
0.0GlyXaa: 0.0 ± 0.0
His
2.243HisAla: 2.243 ± 0.338
0.236HisCys: 0.236 ± 0.114
0.826HisAsp: 0.826 ± 0.266
1.122HisGlu: 1.122 ± 0.266
0.767HisPhe: 0.767 ± 0.216
2.361HisGly: 2.361 ± 0.362
0.354HisHis: 0.354 ± 0.139
0.472HisIle: 0.472 ± 0.135
0.708HisLys: 0.708 ± 0.253
1.003HisLeu: 1.003 ± 0.219
0.531HisMet: 0.531 ± 0.175
0.59HisAsn: 0.59 ± 0.193
1.771HisPro: 1.771 ± 0.32
0.531HisGln: 0.531 ± 0.155
1.122HisArg: 1.122 ± 0.249
0.767HisSer: 0.767 ± 0.201
0.944HisThr: 0.944 ± 0.24
1.003HisVal: 1.003 ± 0.236
0.354HisTrp: 0.354 ± 0.14
0.708HisTyr: 0.708 ± 0.225
0.0HisXaa: 0.0 ± 0.0
Ile
4.663IleAla: 4.663 ± 0.627
0.649IleCys: 0.649 ± 0.216
2.479IleAsp: 2.479 ± 0.412
2.42IleGlu: 2.42 ± 0.359
1.535IlePhe: 1.535 ± 0.404
3.365IleGly: 3.365 ± 0.567
0.944IleHis: 0.944 ± 0.224
1.358IleIle: 1.358 ± 0.285
2.656IleLys: 2.656 ± 0.499
2.597IleLeu: 2.597 ± 0.341
1.063IleMet: 1.063 ± 0.237
1.712IleAsn: 1.712 ± 0.295
2.243IlePro: 2.243 ± 0.391
1.417IleGln: 1.417 ± 0.296
2.774IleArg: 2.774 ± 0.418
1.712IleSer: 1.712 ± 0.415
2.479IleThr: 2.479 ± 0.374
3.01IleVal: 3.01 ± 0.414
0.767IleTrp: 0.767 ± 0.152
0.885IleTyr: 0.885 ± 0.194
0.0IleXaa: 0.0 ± 0.0
Lys
7.851LysAla: 7.851 ± 1.752
0.649LysCys: 0.649 ± 0.205
2.951LysAsp: 2.951 ± 0.479
3.01LysGlu: 3.01 ± 0.501
1.299LysPhe: 1.299 ± 0.282
4.25LysGly: 4.25 ± 0.495
1.063LysHis: 1.063 ± 0.33
2.715LysIle: 2.715 ± 0.378
3.896LysLys: 3.896 ± 1.206
5.017LysLeu: 5.017 ± 0.442
1.948LysMet: 1.948 ± 0.377
1.358LysAsn: 1.358 ± 0.225
3.247LysPro: 3.247 ± 0.555
1.712LysGln: 1.712 ± 0.383
3.01LysArg: 3.01 ± 0.447
2.243LysSer: 2.243 ± 0.414
3.955LysThr: 3.955 ± 0.666
3.719LysVal: 3.719 ± 0.485
0.767LysTrp: 0.767 ± 0.261
1.358LysTyr: 1.358 ± 0.273
0.0LysXaa: 0.0 ± 0.0
Leu
9.563LeuAla: 9.563 ± 0.727
0.708LeuCys: 0.708 ± 0.207
4.545LeuAsp: 4.545 ± 0.66
4.958LeuGlu: 4.958 ± 0.654
1.24LeuPhe: 1.24 ± 0.284
6.552LeuGly: 6.552 ± 0.786
1.594LeuHis: 1.594 ± 0.321
3.01LeuIle: 3.01 ± 0.442
4.191LeuLys: 4.191 ± 0.44
4.486LeuLeu: 4.486 ± 0.637
1.594LeuMet: 1.594 ± 0.305
2.243LeuAsn: 2.243 ± 0.372
4.25LeuPro: 4.25 ± 0.443
1.594LeuGln: 1.594 ± 0.307
5.667LeuArg: 5.667 ± 0.59
4.073LeuSer: 4.073 ± 0.551
4.958LeuThr: 4.958 ± 0.578
4.25LeuVal: 4.25 ± 0.533
1.771LeuTrp: 1.771 ± 0.444
1.594LeuTyr: 1.594 ± 0.303
0.0LeuXaa: 0.0 ± 0.0
Met
2.42MetAla: 2.42 ± 0.379
0.177MetCys: 0.177 ± 0.096
1.712MetAsp: 1.712 ± 0.286
1.122MetGlu: 1.122 ± 0.29
0.767MetPhe: 0.767 ± 0.204
2.007MetGly: 2.007 ± 0.3
0.295MetHis: 0.295 ± 0.113
1.063MetIle: 1.063 ± 0.253
1.771MetLys: 1.771 ± 0.314
1.889MetLeu: 1.889 ± 0.335
0.177MetMet: 0.177 ± 0.081
0.531MetAsn: 0.531 ± 0.136
1.889MetPro: 1.889 ± 0.412
0.472MetGln: 0.472 ± 0.16
1.417MetArg: 1.417 ± 0.277
1.948MetSer: 1.948 ± 0.338
2.066MetThr: 2.066 ± 0.354
1.24MetVal: 1.24 ± 0.303
0.236MetTrp: 0.236 ± 0.128
0.708MetTyr: 0.708 ± 0.18
0.0MetXaa: 0.0 ± 0.0
Asn
4.014AsnAla: 4.014 ± 0.705
0.413AsnCys: 0.413 ± 0.214
2.125AsnAsp: 2.125 ± 0.35
1.83AsnGlu: 1.83 ± 0.298
1.358AsnPhe: 1.358 ± 0.425
3.188AsnGly: 3.188 ± 0.422
0.472AsnHis: 0.472 ± 0.112
1.003AsnIle: 1.003 ± 0.275
1.003AsnLys: 1.003 ± 0.231
2.42AsnLeu: 2.42 ± 0.484
0.531AsnMet: 0.531 ± 0.181
0.944AsnAsn: 0.944 ± 0.254
2.597AsnPro: 2.597 ± 0.412
0.826AsnGln: 0.826 ± 0.213
1.417AsnArg: 1.417 ± 0.276
1.299AsnSer: 1.299 ± 0.309
1.889AsnThr: 1.889 ± 0.426
1.712AsnVal: 1.712 ± 0.383
0.413AsnTrp: 0.413 ± 0.145
0.472AsnTyr: 0.472 ± 0.193
0.0AsnXaa: 0.0 ± 0.0
Pro
5.726ProAla: 5.726 ± 0.757
0.708ProCys: 0.708 ± 0.21
3.601ProAsp: 3.601 ± 0.492
4.545ProGlu: 4.545 ± 0.489
2.007ProPhe: 2.007 ± 0.323
7.083ProGly: 7.083 ± 0.654
1.063ProHis: 1.063 ± 0.285
1.594ProIle: 1.594 ± 0.29
2.951ProLys: 2.951 ± 0.459
3.483ProLeu: 3.483 ± 0.511
0.885ProMet: 0.885 ± 0.211
1.535ProAsn: 1.535 ± 0.275
3.306ProPro: 3.306 ± 0.534
1.594ProGln: 1.594 ± 0.298
2.951ProArg: 2.951 ± 0.499
2.774ProSer: 2.774 ± 0.527
2.538ProThr: 2.538 ± 0.37
4.545ProVal: 4.545 ± 0.664
1.358ProTrp: 1.358 ± 0.341
1.712ProTyr: 1.712 ± 0.296
0.0ProXaa: 0.0 ± 0.0
Gln
4.427GlnAla: 4.427 ± 0.63
0.413GlnCys: 0.413 ± 0.194
1.889GlnAsp: 1.889 ± 0.283
1.417GlnGlu: 1.417 ± 0.285
0.944GlnPhe: 0.944 ± 0.234
2.951GlnGly: 2.951 ± 0.56
1.003GlnHis: 1.003 ± 0.275
1.83GlnIle: 1.83 ± 0.349
2.361GlnLys: 2.361 ± 0.535
2.302GlnLeu: 2.302 ± 0.398
1.181GlnMet: 1.181 ± 0.238
1.063GlnAsn: 1.063 ± 0.289
1.535GlnPro: 1.535 ± 0.351
2.007GlnGln: 2.007 ± 0.385
2.479GlnArg: 2.479 ± 0.409
1.594GlnSer: 1.594 ± 0.305
2.066GlnThr: 2.066 ± 0.385
1.889GlnVal: 1.889 ± 0.309
0.59GlnTrp: 0.59 ± 0.193
0.708GlnTyr: 0.708 ± 0.179
0.0GlnXaa: 0.0 ± 0.0
Arg
7.142ArgAla: 7.142 ± 0.724
0.885ArgCys: 0.885 ± 0.27
3.483ArgAsp: 3.483 ± 0.466
5.431ArgGlu: 5.431 ± 0.699
1.771ArgPhe: 1.771 ± 0.443
4.545ArgGly: 4.545 ± 0.552
0.767ArgHis: 0.767 ± 0.18
3.069ArgIle: 3.069 ± 0.399
3.896ArgLys: 3.896 ± 0.605
3.837ArgLeu: 3.837 ± 0.483
1.594ArgMet: 1.594 ± 0.348
1.535ArgAsn: 1.535 ± 0.274
3.837ArgPro: 3.837 ± 0.569
3.01ArgGln: 3.01 ± 0.331
5.135ArgArg: 5.135 ± 0.762
3.188ArgSer: 3.188 ± 0.432
3.719ArgThr: 3.719 ± 0.516
4.545ArgVal: 4.545 ± 0.539
1.653ArgTrp: 1.653 ± 0.359
2.184ArgTyr: 2.184 ± 0.429
0.0ArgXaa: 0.0 ± 0.0
Ser
5.254SerAla: 5.254 ± 0.764
0.236SerCys: 0.236 ± 0.122
2.892SerAsp: 2.892 ± 0.449
2.715SerGlu: 2.715 ± 0.443
1.653SerPhe: 1.653 ± 0.286
6.316SerGly: 6.316 ± 0.676
0.413SerHis: 0.413 ± 0.161
2.42SerIle: 2.42 ± 0.394
2.479SerLys: 2.479 ± 0.46
3.247SerLeu: 3.247 ± 0.365
1.003SerMet: 1.003 ± 0.222
2.066SerAsn: 2.066 ± 0.379
2.951SerPro: 2.951 ± 0.406
1.889SerGln: 1.889 ± 0.312
2.125SerArg: 2.125 ± 0.339
1.653SerSer: 1.653 ± 0.35
2.42SerThr: 2.42 ± 0.383
3.778SerVal: 3.778 ± 0.448
1.417SerTrp: 1.417 ± 0.275
1.535SerTyr: 1.535 ± 0.345
0.0SerXaa: 0.0 ± 0.0
Thr
7.674ThrAla: 7.674 ± 0.784
0.826ThrCys: 0.826 ± 0.288
2.951ThrAsp: 2.951 ± 0.465
3.069ThrGlu: 3.069 ± 0.45
1.476ThrPhe: 1.476 ± 0.277
5.962ThrGly: 5.962 ± 0.562
0.944ThrHis: 0.944 ± 0.173
2.774ThrIle: 2.774 ± 0.389
2.479ThrLys: 2.479 ± 0.37
5.017ThrLeu: 5.017 ± 0.573
0.767ThrMet: 0.767 ± 0.145
1.535ThrAsn: 1.535 ± 0.269
4.604ThrPro: 4.604 ± 0.536
1.476ThrGln: 1.476 ± 0.291
3.365ThrArg: 3.365 ± 0.406
3.483ThrSer: 3.483 ± 0.454
3.719ThrThr: 3.719 ± 0.46
4.486ThrVal: 4.486 ± 0.597
1.594ThrTrp: 1.594 ± 0.38
2.302ThrTyr: 2.302 ± 0.525
0.0ThrXaa: 0.0 ± 0.0
Val
7.91ValAla: 7.91 ± 0.544
0.354ValCys: 0.354 ± 0.164
3.837ValAsp: 3.837 ± 0.604
4.014ValGlu: 4.014 ± 0.474
1.653ValPhe: 1.653 ± 0.29
5.313ValGly: 5.313 ± 0.65
1.181ValHis: 1.181 ± 0.276
3.896ValIle: 3.896 ± 0.456
3.247ValLys: 3.247 ± 0.421
5.313ValLeu: 5.313 ± 0.619
2.007ValMet: 2.007 ± 0.293
2.42ValAsn: 2.42 ± 0.51
4.073ValPro: 4.073 ± 0.513
1.83ValGln: 1.83 ± 0.318
4.781ValArg: 4.781 ± 0.562
3.069ValSer: 3.069 ± 0.425
4.486ValThr: 4.486 ± 0.727
5.135ValVal: 5.135 ± 0.659
1.948ValTrp: 1.948 ± 0.358
2.715ValTyr: 2.715 ± 0.365
0.0ValXaa: 0.0 ± 0.0
Trp
2.833TrpAla: 2.833 ± 0.464
0.236TrpCys: 0.236 ± 0.111
2.125TrpAsp: 2.125 ± 0.361
0.944TrpGlu: 0.944 ± 0.2
0.708TrpPhe: 0.708 ± 0.226
1.358TrpGly: 1.358 ± 0.269
0.531TrpHis: 0.531 ± 0.182
1.417TrpIle: 1.417 ± 0.263
1.181TrpLys: 1.181 ± 0.202
1.948TrpLeu: 1.948 ± 0.444
0.354TrpMet: 0.354 ± 0.177
0.885TrpAsn: 0.885 ± 0.328
0.826TrpPro: 0.826 ± 0.257
0.885TrpGln: 0.885 ± 0.203
1.417TrpArg: 1.417 ± 0.266
1.417TrpSer: 1.417 ± 0.357
1.476TrpThr: 1.476 ± 0.285
1.417TrpVal: 1.417 ± 0.306
0.59TrpTrp: 0.59 ± 0.219
0.354TrpTyr: 0.354 ± 0.155
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.774TyrAla: 2.774 ± 0.343
0.354TyrCys: 0.354 ± 0.183
1.653TyrAsp: 1.653 ± 0.258
1.653TyrGlu: 1.653 ± 0.31
0.767TyrPhe: 0.767 ± 0.185
2.833TyrGly: 2.833 ± 0.422
0.354TyrHis: 0.354 ± 0.148
0.59TyrIle: 0.59 ± 0.194
1.24TyrLys: 1.24 ± 0.285
1.83TyrLeu: 1.83 ± 0.31
0.531TyrMet: 0.531 ± 0.158
1.003TyrAsn: 1.003 ± 0.216
1.299TyrPro: 1.299 ± 0.332
1.003TyrGln: 1.003 ± 0.256
2.42TyrArg: 2.42 ± 0.371
2.007TyrSer: 2.007 ± 0.341
2.42TyrThr: 2.42 ± 0.503
1.712TyrVal: 1.712 ± 0.272
0.649TyrTrp: 0.649 ± 0.185
0.472TyrTyr: 0.472 ± 0.227
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 81 proteins (16942 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski