Zostera marina (Eelgrass)

Taxonomy: cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliopsida; Mesangiospermae; Liliopsida; Alismatales; Zosteraceae; Zostera

Average proteome isoelectric point is 6.8

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 20559 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0K9PTQ4|A0A0K9PTQ4_ZOSMR Uncharacterized protein OS=Zostera marina OX=29655 GN=ZOSMA_166G00610 PE=3 SV=1
MM1 pKa = 7.96DD2 pKa = 5.29KK3 pKa = 10.33EE4 pKa = 4.21QKK6 pKa = 10.55VYY8 pKa = 11.11AGNSGKK14 pKa = 10.26AVSAGNFEE22 pKa = 4.63TNQKK26 pKa = 9.8HH27 pKa = 3.56VTYY30 pKa = 10.06WEE32 pKa = 4.35DD33 pKa = 3.59KK34 pKa = 9.66NTGEE38 pKa = 4.46YY39 pKa = 9.86IRR41 pKa = 11.84NTVTEE46 pKa = 4.01KK47 pKa = 11.07SNPNEE52 pKa = 3.6SRR54 pKa = 11.84QYY56 pKa = 9.6RR57 pKa = 11.84TTTTTVGNKK66 pKa = 7.89STGDD70 pKa = 3.61YY71 pKa = 10.34VVKK74 pKa = 10.66QSQQVCKK81 pKa = 11.02NNDD84 pKa = 3.07CRR86 pKa = 11.84TLNDD90 pKa = 3.88VIDD93 pKa = 5.01DD94 pKa = 4.29GSDD97 pKa = 3.29EE98 pKa = 4.32EE99 pKa = 5.34GVDD102 pKa = 4.84NGGCDD107 pKa = 3.07EE108 pKa = 4.4GVDD111 pKa = 4.4YY112 pKa = 11.52VRR114 pKa = 11.84DD115 pKa = 3.95DD116 pKa = 3.45QGSEE120 pKa = 4.0DD121 pKa = 4.75GDD123 pKa = 4.01GVDD126 pKa = 4.56CGGGGEE132 pKa = 4.54DD133 pKa = 5.4DD134 pKa = 4.98GYY136 pKa = 11.1DD137 pKa = 3.67YY138 pKa = 11.81SDD140 pKa = 4.51GYY142 pKa = 11.18SDD144 pKa = 5.68GVDD147 pKa = 3.6CGGGDD152 pKa = 3.8DD153 pKa = 5.93GSDD156 pKa = 2.89FDD158 pKa = 5.82YY159 pKa = 11.53YY160 pKa = 11.24DD161 pKa = 4.1GYY163 pKa = 11.89

Molecular weight: 17.66 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0K9PAK4|A0A0K9PAK4_ZOSMR Uncharacterized protein OS=Zostera marina OX=29655 GN=ZOSMA_2G00130 PE=4 SV=1
MM1 pKa = 7.1RR2 pKa = 11.84AKK4 pKa = 9.27WKK6 pKa = 9.53KK7 pKa = 9.88KK8 pKa = 8.64RR9 pKa = 11.84MRR11 pKa = 11.84RR12 pKa = 11.84LKK14 pKa = 10.08RR15 pKa = 11.84KK16 pKa = 8.21RR17 pKa = 11.84RR18 pKa = 11.84KK19 pKa = 8.46MRR21 pKa = 11.84QRR23 pKa = 11.84SKK25 pKa = 11.41

Molecular weight: 3.43 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

20358

201

20559

7987506

8

5302

388.5

43.55

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.869 ± 0.018

1.87 ± 0.009

5.575 ± 0.014

6.201 ± 0.018

4.387 ± 0.011

6.273 ± 0.019

2.446 ± 0.007

6.096 ± 0.011

6.333 ± 0.018

9.356 ± 0.017

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.607 ± 0.008

4.849 ± 0.013

4.559 ± 0.016

3.451 ± 0.013

5.243 ± 0.013

9.185 ± 0.02

5.176 ± 0.011

6.466 ± 0.012

1.216 ± 0.006

2.843 ± 0.009
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski