Staphylococcus phage phiSP44-1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Coventryvirus; Staphylococcus virus phiSP44-1

Average proteome isoelectric point is 6.29

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 66 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A499SJ85|A0A499SJ85_9CAUD Holliday junction resolvase OS=Staphylococcus phage phiSP44-1 OX=2491321 PE=4 SV=1
MM1 pKa = 7.28YY2 pKa = 10.07FPNGEE7 pKa = 4.22EE8 pKa = 3.69YY9 pKa = 10.84SGIIEE14 pKa = 4.0VEE16 pKa = 3.89GFKK19 pKa = 10.39FRR21 pKa = 11.84KK22 pKa = 9.48YY23 pKa = 7.14VTRR26 pKa = 11.84QDD28 pKa = 3.3DD29 pKa = 4.19HH30 pKa = 8.96ILIEE34 pKa = 4.46VTNMTYY40 pKa = 10.95GVIAEE45 pKa = 4.28TNVYY49 pKa = 10.09NLSDD53 pKa = 3.27VDD55 pKa = 3.43IAQEE59 pKa = 4.0VLSAAVYY66 pKa = 10.19DD67 pKa = 4.33YY68 pKa = 11.31IEE70 pKa = 4.41NQTDD74 pKa = 3.61EE75 pKa = 4.58LDD77 pKa = 3.78KK78 pKa = 11.5IMAHH82 pKa = 7.19LIKK85 pKa = 10.87NN86 pKa = 3.75

Molecular weight: 9.96 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A499SQM0|A0A499SQM0_9CAUD Capsid and scaffold protein OS=Staphylococcus phage phiSP44-1 OX=2491321 PE=4 SV=1
MM1 pKa = 7.87DD2 pKa = 5.12LSEE5 pKa = 5.14SIKK8 pKa = 10.32QRR10 pKa = 11.84YY11 pKa = 6.72RR12 pKa = 11.84TDD14 pKa = 3.22TAGKK18 pKa = 10.19SPTEE22 pKa = 3.87LQRR25 pKa = 11.84EE26 pKa = 4.08LRR28 pKa = 11.84KK29 pKa = 10.09RR30 pKa = 11.84GVRR33 pKa = 11.84GFVVNVSHH41 pKa = 6.88NRR43 pKa = 11.84VTMLVDD49 pKa = 3.12RR50 pKa = 11.84RR51 pKa = 11.84DD52 pKa = 3.27VKK54 pKa = 10.88RR55 pKa = 11.84NKK57 pKa = 9.93EE58 pKa = 4.02CMRR61 pKa = 4.66

Molecular weight: 7.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

66

0

66

13058

53

1039

197.8

22.63

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.981 ± 0.366

0.398 ± 0.064

6.525 ± 0.35

7.513 ± 0.472

4.35 ± 0.197

5.33 ± 0.272

1.892 ± 0.17

7.367 ± 0.281

9.075 ± 0.334

8.087 ± 0.272

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.428 ± 0.139

6.494 ± 0.235

2.711 ± 0.206

3.806 ± 0.252

4.12 ± 0.191

6.035 ± 0.291

5.95 ± 0.233

6.663 ± 0.255

1.003 ± 0.141

4.273 ± 0.303
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski