Thermus phage phiYS40

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.46

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 170 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0MN21|A0MN21_9CAUD Portal protein OS=Thermus phage phiYS40 OX=407392 GN=YS40_003 PE=4 SV=1
MM1 pKa = 7.34IQLKK5 pKa = 10.62LLDD8 pKa = 3.41QDD10 pKa = 4.63FEE12 pKa = 5.03VEE14 pKa = 3.74VDD16 pKa = 3.49DD17 pKa = 6.31RR18 pKa = 11.84YY19 pKa = 11.24LILYY23 pKa = 10.33SNDD26 pKa = 3.84DD27 pKa = 3.71FCSSPKK33 pKa = 9.68TMRR36 pKa = 11.84FVGFEE41 pKa = 3.71IYY43 pKa = 10.41TFDD46 pKa = 5.14NVFCGIKK53 pKa = 9.68IRR55 pKa = 11.84LEE57 pKa = 3.96KK58 pKa = 10.75LDD60 pKa = 3.75DD61 pKa = 4.19RR62 pKa = 11.84KK63 pKa = 10.81LEE65 pKa = 4.32SIILVDD71 pKa = 4.08EE72 pKa = 4.3YY73 pKa = 11.4NYY75 pKa = 10.63EE76 pKa = 3.82IDD78 pKa = 3.48LSSFVSSYY86 pKa = 10.21II87 pKa = 3.51

Molecular weight: 10.38 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0MNG7|A0MNG7_9CAUD Uncharacterized protein OS=Thermus phage phiYS40 OX=407392 GN=YS40_149 PE=4 SV=1
MM1 pKa = 6.72QTLKK5 pKa = 9.31TWYY8 pKa = 7.85QNKK11 pKa = 8.3MSVYY15 pKa = 10.18RR16 pKa = 11.84EE17 pKa = 3.96TNKK20 pKa = 10.09TIRR23 pKa = 11.84DD24 pKa = 3.63IQNLRR29 pKa = 11.84TFYY32 pKa = 10.88YY33 pKa = 10.6LMKK36 pKa = 10.39AEE38 pKa = 4.33HH39 pKa = 6.76GEE41 pKa = 4.02YY42 pKa = 10.15AIKK45 pKa = 10.42DD46 pKa = 3.45WNTLFRR52 pKa = 11.84KK53 pKa = 9.22FLKK56 pKa = 10.53EE57 pKa = 3.68QGLLL61 pKa = 3.63

Molecular weight: 7.6 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

170

0

170

47943

42

1744

282.0

32.66

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.376 ± 0.19

0.538 ± 0.058

5.336 ± 0.11

8.558 ± 0.342

6.681 ± 0.182

4.422 ± 0.181

1.197 ± 0.071

7.953 ± 0.149

9.077 ± 0.281

9.883 ± 0.151

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.55 ± 0.097

6.401 ± 0.208

3.173 ± 0.108

2.58 ± 0.129

3.75 ± 0.136

7.49 ± 0.209

4.783 ± 0.167

6.241 ± 0.144

0.526 ± 0.04

5.486 ± 0.208
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski