Amino acid dipepetide frequency for Sphingomonas wittichii (strain RW1 / DSM 6014 / JCM 10273)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	20.647AlaAla: 20.647 ± 0.167	1.068AlaCys: 1.068 ± 0.026	8.216AlaAsp: 8.216 ± 0.075	7.857AlaGlu: 7.857 ± 0.076	4.472AlaPhe: 4.472 ± 0.049	12.268AlaGly: 12.268 ± 0.103	2.415AlaHis: 2.415 ± 0.041	7.285AlaIle: 7.285 ± 0.074	3.5AlaLys: 3.5 ± 0.052	13.754AlaLeu: 13.754 ± 0.113	3.974AlaMet: 3.974 ± 0.055	2.858AlaAsn: 2.858 ± 0.039	6.637AlaPro: 6.637 ± 0.068	4.041AlaGln: 4.041 ± 0.054	10.803AlaArg: 10.803 ± 0.089	6.323AlaSer: 6.323 ± 0.062	6.505AlaThr: 6.505 ± 0.055	8.748AlaVal: 8.748 ± 0.072	1.694AlaTrp: 1.694 ± 0.035	2.785AlaTyr: 2.785 ± 0.042	0.0AlaXaa: 0.0 ± 0.0
Cys	1.035CysAla: 1.035 ± 0.022	0.109CysCys: 0.109 ± 0.008	0.565CysAsp: 0.565 ± 0.015	0.381CysGlu: 0.381 ± 0.015	0.328CysPhe: 0.328 ± 0.012	0.869CysGly: 0.869 ± 0.022	0.243CysHis: 0.243 ± 0.015	0.362CysIle: 0.362 ± 0.015	0.136CysLys: 0.136 ± 0.009	0.722CysLeu: 0.722 ± 0.02	0.161CysMet: 0.161 ± 0.009	0.197CysAsn: 0.197 ± 0.011	0.496CysPro: 0.496 ± 0.016	0.19CysGln: 0.19 ± 0.011	0.63CysArg: 0.63 ± 0.019	0.446CysSer: 0.446 ± 0.015	0.363CysThr: 0.363 ± 0.014	0.556CysVal: 0.556 ± 0.015	0.127CysTrp: 0.127 ± 0.01	0.186CysTyr: 0.186 ± 0.01	0.0CysXaa: 0.0 ± 0.0
Asp	8.024AspAla: 8.024 ± 0.074	0.46AspCys: 0.46 ± 0.017	3.601AspAsp: 3.601 ± 0.053	3.394AspGlu: 3.394 ± 0.056	2.201AspPhe: 2.201 ± 0.041	6.189AspGly: 6.189 ± 0.058	1.511AspHis: 1.511 ± 0.029	3.095AspIle: 3.095 ± 0.046	1.563AspLys: 1.563 ± 0.032	5.831AspLeu: 5.831 ± 0.064	1.408AspMet: 1.408 ± 0.028	1.23AspAsn: 1.23 ± 0.026	4.194AspPro: 4.194 ± 0.045	1.786AspGln: 1.786 ± 0.04	5.898AspArg: 5.898 ± 0.056	2.25AspSer: 2.25 ± 0.035	2.272AspThr: 2.272 ± 0.039	4.009AspVal: 4.009 ± 0.044	1.086AspTrp: 1.086 ± 0.026	1.847AspTyr: 1.847 ± 0.036	0.0AspXaa: 0.0 ± 0.0
Glu	7.958GluAla: 7.958 ± 0.081	0.308GluCys: 0.308 ± 0.013	2.597GluAsp: 2.597 ± 0.04	2.931GluGlu: 2.931 ± 0.053	1.372GluPhe: 1.372 ± 0.029	4.466GluGly: 4.466 ± 0.062	1.111GluHis: 1.111 ± 0.028	2.982GluIle: 2.982 ± 0.044	1.7GluLys: 1.7 ± 0.035	5.1GluLeu: 5.1 ± 0.063	1.349GluMet: 1.349 ± 0.026	1.144GluAsn: 1.144 ± 0.028	2.564GluPro: 2.564 ± 0.043	1.996GluGln: 1.996 ± 0.029	5.107GluArg: 5.107 ± 0.061	1.948GluSer: 1.948 ± 0.029	2.98GluThr: 2.98 ± 0.047	3.458GluVal: 3.458 ± 0.058	0.685GluTrp: 0.685 ± 0.02	0.831GluTyr: 0.831 ± 0.022	0.0GluXaa: 0.0 ± 0.0
Phe	4.701PheAla: 4.701 ± 0.055	0.328PheCys: 0.328 ± 0.015	2.959PheAsp: 2.959 ± 0.045	2.023PheGlu: 2.023 ± 0.033	1.229PhePhe: 1.229 ± 0.027	3.799PheGly: 3.799 ± 0.046	0.801PheHis: 0.801 ± 0.023	1.333PheIle: 1.333 ± 0.027	0.847PheLys: 0.847 ± 0.025	3.033PheLeu: 3.033 ± 0.049	0.716PheMet: 0.716 ± 0.02	1.011PheAsn: 1.011 ± 0.026	1.612PhePro: 1.612 ± 0.035	0.888PheGln: 0.888 ± 0.023	2.371PheArg: 2.371 ± 0.035	2.016PheSer: 2.016 ± 0.033	1.951PheThr: 1.951 ± 0.036	2.619PheVal: 2.619 ± 0.039	0.46PheTrp: 0.46 ± 0.017	0.893PheTyr: 0.893 ± 0.024	0.0PheXaa: 0.0 ± 0.0
Gly	10.559GlyAla: 10.559 ± 0.097	0.879GlyCys: 0.879 ± 0.022	5.231GlyAsp: 5.231 ± 0.063	4.835GlyGlu: 4.835 ± 0.058	3.854GlyPhe: 3.854 ± 0.053	8.758GlyGly: 8.758 ± 0.119	2.06GlyHis: 2.06 ± 0.036	4.569GlyIle: 4.569 ± 0.05	2.959GlyLys: 2.959 ± 0.041	9.129GlyLeu: 9.129 ± 0.077	2.265GlyMet: 2.265 ± 0.038	2.126GlyAsn: 2.126 ± 0.041	4.146GlyPro: 4.146 ± 0.068	2.925GlyGln: 2.925 ± 0.046	7.538GlyArg: 7.538 ± 0.066	4.882GlySer: 4.882 ± 0.058	4.558GlyThr: 4.558 ± 0.053	6.26GlyVal: 6.26 ± 0.065	1.654GlyTrp: 1.654 ± 0.034	2.518GlyTyr: 2.518 ± 0.041	0.0GlyXaa: 0.0 ± 0.0
His	2.471HisAla: 2.471 ± 0.039	0.237HisCys: 0.237 ± 0.012	1.335HisAsp: 1.335 ± 0.029	0.944HisGlu: 0.944 ± 0.022	0.847HisPhe: 0.847 ± 0.023	2.234HisGly: 2.234 ± 0.036	0.581HisHis: 0.581 ± 0.017	0.906HisIle: 0.906 ± 0.021	0.412HisLys: 0.412 ± 0.016	1.974HisLeu: 1.974 ± 0.035	0.504HisMet: 0.504 ± 0.016	0.444HisAsn: 0.444 ± 0.015	1.405HisPro: 1.405 ± 0.026	0.543HisGln: 0.543 ± 0.018	1.728HisArg: 1.728 ± 0.035	0.94HisSer: 0.94 ± 0.024	0.598HisThr: 0.598 ± 0.022	1.657HisVal: 1.657 ± 0.032	0.361HisTrp: 0.361 ± 0.013	0.613HisTyr: 0.613 ± 0.018	0.0HisXaa: 0.0 ± 0.0
Ile	8.026IleAla: 8.026 ± 0.074	0.436IleCys: 0.436 ± 0.018	4.212IleAsp: 4.212 ± 0.059	3.643IleGlu: 3.643 ± 0.052	1.547IlePhe: 1.547 ± 0.029	5.368IleGly: 5.368 ± 0.061	0.939IleHis: 0.939 ± 0.019	1.858IleIle: 1.858 ± 0.038	1.103IleLys: 1.103 ± 0.026	3.963IleLeu: 3.963 ± 0.051	0.847IleMet: 0.847 ± 0.023	1.197IleAsn: 1.197 ± 0.026	2.218IlePro: 2.218 ± 0.038	1.119IleGln: 1.119 ± 0.026	3.433IleArg: 3.433 ± 0.04	2.55IleSer: 2.55 ± 0.04	2.301IleThr: 2.301 ± 0.04	4.299IleVal: 4.299 ± 0.052	0.565IleTrp: 0.565 ± 0.019	0.964IleTyr: 0.964 ± 0.026	0.0IleXaa: 0.0 ± 0.0
Lys	3.838LysAla: 3.838 ± 0.051	0.123LysCys: 0.123 ± 0.008	1.409LysAsp: 1.409 ± 0.029	1.061LysGlu: 1.061 ± 0.026	0.704LysPhe: 0.704 ± 0.02	2.488LysGly: 2.488 ± 0.042	0.426LysHis: 0.426 ± 0.016	1.346LysIle: 1.346 ± 0.028	0.832LysLys: 0.832 ± 0.025	2.892LysLeu: 2.892 ± 0.042	0.613LysMet: 0.613 ± 0.02	0.583LysAsn: 0.583 ± 0.019	1.845LysPro: 1.845 ± 0.033	0.774LysGln: 0.774 ± 0.021	2.082LysArg: 2.082 ± 0.034	1.329LysSer: 1.329 ± 0.034	1.404LysThr: 1.404 ± 0.029	1.988LysVal: 1.988 ± 0.036	0.331LysTrp: 0.331 ± 0.012	0.537LysTyr: 0.537 ± 0.019	0.0LysXaa: 0.0 ± 0.0
Leu	14.024LeuAla: 14.024 ± 0.123	0.869LeuCys: 0.869 ± 0.023	6.666LeuAsp: 6.666 ± 0.065	4.332LeuGlu: 4.332 ± 0.059	3.722LeuPhe: 3.722 ± 0.056	8.604LeuGly: 8.604 ± 0.073	1.895LeuHis: 1.895 ± 0.032	4.734LeuIle: 4.734 ± 0.059	2.69LeuLys: 2.69 ± 0.043	9.715LeuLeu: 9.715 ± 0.125	2.037LeuMet: 2.037 ± 0.037	2.171LeuAsn: 2.171 ± 0.04	5.749LeuPro: 5.749 ± 0.066	2.38LeuGln: 2.38 ± 0.045	7.315LeuArg: 7.315 ± 0.069	5.988LeuSer: 5.988 ± 0.062	5.091LeuThr: 5.091 ± 0.058	6.99LeuVal: 6.99 ± 0.069	1.242LeuTrp: 1.242 ± 0.029	1.916LeuTyr: 1.916 ± 0.032	0.0LeuXaa: 0.0 ± 0.0
Met	3.348MetAla: 3.348 ± 0.047	0.159MetCys: 0.159 ± 0.009	1.069MetAsp: 1.069 ± 0.026	1.023MetGlu: 1.023 ± 0.028	0.639MetPhe: 0.639 ± 0.019	1.776MetGly: 1.776 ± 0.036	0.451MetHis: 0.451 ± 0.015	1.416MetIle: 1.416 ± 0.029	0.809MetLys: 0.809 ± 0.021	2.565MetLeu: 2.565 ± 0.043	0.652MetMet: 0.652 ± 0.022	0.596MetAsn: 0.596 ± 0.017	1.48MetPro: 1.48 ± 0.03	0.629MetGln: 0.629 ± 0.019	1.84MetArg: 1.84 ± 0.034	1.436MetSer: 1.436 ± 0.028	1.77MetThr: 1.77 ± 0.03	1.58MetVal: 1.58 ± 0.033	0.206MetTrp: 0.206 ± 0.012	0.28MetTyr: 0.28 ± 0.014	0.0MetXaa: 0.0 ± 0.0
Asn	2.973AsnAla: 2.973 ± 0.043	0.224AsnCys: 0.224 ± 0.013	1.302AsnAsp: 1.302 ± 0.032	0.97AsnGlu: 0.97 ± 0.025	0.87AsnPhe: 0.87 ± 0.026	2.278AsnGly: 2.278 ± 0.041	0.456AsnHis: 0.456 ± 0.015	1.257AsnIle: 1.257 ± 0.032	0.535AsnLys: 0.535 ± 0.019	2.365AsnLeu: 2.365 ± 0.035	0.522AsnMet: 0.522 ± 0.016	0.61AsnAsn: 0.61 ± 0.019	1.616AsnPro: 1.616 ± 0.036	0.661AsnGln: 0.661 ± 0.019	1.818AsnArg: 1.818 ± 0.035	1.15AsnSer: 1.15 ± 0.031	0.944AsnThr: 0.944 ± 0.026	1.738AsnVal: 1.738 ± 0.036	0.405AsnTrp: 0.405 ± 0.017	0.699AsnTyr: 0.699 ± 0.02	0.0AsnXaa: 0.0 ± 0.0
Pro	7.866ProAla: 7.866 ± 0.085	0.362ProCys: 0.362 ± 0.015	3.948ProAsp: 3.948 ± 0.049	3.324ProGlu: 3.324 ± 0.048	2.032ProPhe: 2.032 ± 0.031	5.186ProGly: 5.186 ± 0.059	1.097ProHis: 1.097 ± 0.027	2.617ProIle: 2.617 ± 0.039	1.466ProLys: 1.466 ± 0.03	5.046ProLeu: 5.046 ± 0.06	1.245ProMet: 1.245 ± 0.028	1.27ProAsn: 1.27 ± 0.03	3.113ProPro: 3.113 ± 0.068	1.584ProGln: 1.584 ± 0.03	3.463ProArg: 3.463 ± 0.047	2.874ProSer: 2.874 ± 0.042	2.582ProThr: 2.582 ± 0.039	4.41ProVal: 4.41 ± 0.053	0.703ProTrp: 0.703 ± 0.019	1.108ProTyr: 1.108 ± 0.025	0.0ProXaa: 0.0 ± 0.0
Gln	4.165GlnAla: 4.165 ± 0.048	0.221GlnCys: 0.221 ± 0.01	1.364GlnAsp: 1.364 ± 0.025	1.231GlnGlu: 1.231 ± 0.029	1.018GlnPhe: 1.018 ± 0.023	2.549GlnGly: 2.549 ± 0.032	0.566GlnHis: 0.566 ± 0.016	1.561GlnIle: 1.561 ± 0.034	0.801GlnLys: 0.801 ± 0.021	2.844GlnLeu: 2.844 ± 0.045	0.73GlnMet: 0.73 ± 0.02	0.683GlnAsn: 0.683 ± 0.026	1.801GlnPro: 1.801 ± 0.038	1.046GlnGln: 1.046 ± 0.026	2.417GlnArg: 2.417 ± 0.038	1.508GlnSer: 1.508 ± 0.035	1.393GlnThr: 1.393 ± 0.034	2.108GlnVal: 2.108 ± 0.035	0.391GlnTrp: 0.391 ± 0.015	0.601GlnTyr: 0.601 ± 0.018	0.0GlnXaa: 0.0 ± 0.0
Arg	9.572ArgAla: 9.572 ± 0.088	0.621ArgCys: 0.621 ± 0.02	4.668ArgAsp: 4.668 ± 0.048	3.994ArgGlu: 3.994 ± 0.06	3.391ArgPhe: 3.391 ± 0.049	5.547ArgGly: 5.547 ± 0.061	1.998ArgHis: 1.998 ± 0.035	4.546ArgIle: 4.546 ± 0.052	2.059ArgLys: 2.059 ± 0.036	8.922ArgLeu: 8.922 ± 0.068	2.054ArgMet: 2.054 ± 0.034	1.905ArgAsn: 1.905 ± 0.036	4.413ArgPro: 4.413 ± 0.056	2.521ArgGln: 2.521 ± 0.039	6.927ArgArg: 6.927 ± 0.085	4.078ArgSer: 4.078 ± 0.041	3.823ArgThr: 3.823 ± 0.049	4.789ArgVal: 4.789 ± 0.059	1.33ArgTrp: 1.33 ± 0.03	2.044ArgTyr: 2.044 ± 0.036	0.0ArgXaa: 0.0 ± 0.0
Ser	6.177SerAla: 6.177 ± 0.058	0.423SerCys: 0.423 ± 0.017	2.965SerAsp: 2.965 ± 0.046	2.285SerGlu: 2.285 ± 0.041	2.329SerPhe: 2.329 ± 0.039	5.237SerGly: 5.237 ± 0.067	0.997SerHis: 0.997 ± 0.025	2.783SerIle: 2.783 ± 0.044	1.241SerLys: 1.241 ± 0.027	4.976SerLeu: 4.976 ± 0.065	1.124SerMet: 1.124 ± 0.026	1.264SerAsn: 1.264 ± 0.031	2.946SerPro: 2.946 ± 0.039	1.42SerGln: 1.42 ± 0.031	3.537SerArg: 3.537 ± 0.049	2.734SerSer: 2.734 ± 0.045	2.569SerThr: 2.569 ± 0.041	3.541SerVal: 3.541 ± 0.044	0.744SerTrp: 0.744 ± 0.024	1.507SerTyr: 1.507 ± 0.033	0.0SerXaa: 0.0 ± 0.0
Thr	6.3ThrAla: 6.3 ± 0.065	0.358ThrCys: 0.358 ± 0.014	2.74ThrAsp: 2.74 ± 0.039	2.125ThrGlu: 2.125 ± 0.038	1.599ThrPhe: 1.599 ± 0.03	5.194ThrGly: 5.194 ± 0.068	0.91ThrHis: 0.91 ± 0.022	2.995ThrIle: 2.995 ± 0.046	1.086ThrLys: 1.086 ± 0.027	5.342ThrLeu: 5.342 ± 0.058	1.153ThrMet: 1.153 ± 0.029	1.184ThrAsn: 1.184 ± 0.03	3.33ThrPro: 3.33 ± 0.047	1.304ThrGln: 1.304 ± 0.03	3.352ThrArg: 3.352 ± 0.044	2.498ThrSer: 2.498 ± 0.044	2.535ThrThr: 2.535 ± 0.047	3.936ThrVal: 3.936 ± 0.055	0.539ThrTrp: 0.539 ± 0.019	1.145ThrTyr: 1.145 ± 0.029	0.0ThrXaa: 0.0 ± 0.0
Val	10.008ValAla: 10.008 ± 0.09	0.551ValCys: 0.551 ± 0.019	4.68ValAsp: 4.68 ± 0.06	4.447ValGlu: 4.447 ± 0.05	2.062ValPhe: 2.062 ± 0.035	5.739ValGly: 5.739 ± 0.067	1.388ValHis: 1.388 ± 0.03	3.39ValIle: 3.39 ± 0.049	1.799ValLys: 1.799 ± 0.038	6.186ValLeu: 6.186 ± 0.066	1.525ValMet: 1.525 ± 0.031	1.875ValAsn: 1.875 ± 0.037	3.998ValPro: 3.998 ± 0.049	1.949ValGln: 1.949 ± 0.034	5.481ValArg: 5.481 ± 0.062	3.784ValSer: 3.784 ± 0.038	4.133ValThr: 4.133 ± 0.056	5.299ValVal: 5.299 ± 0.06	0.811ValTrp: 0.811 ± 0.024	1.397ValTyr: 1.397 ± 0.026	0.0ValXaa: 0.0 ± 0.0
Trp	1.341TrpAla: 1.341 ± 0.028	0.116TrpCys: 0.116 ± 0.009	0.74TrpAsp: 0.74 ± 0.021	0.593TrpGlu: 0.593 ± 0.021	0.506TrpPhe: 0.506 ± 0.019	0.921TrpGly: 0.921 ± 0.023	0.357TrpHis: 0.357 ± 0.013	0.719TrpIle: 0.719 ± 0.02	0.481TrpLys: 0.481 ± 0.017	1.634TrpLeu: 1.634 ± 0.034	0.4TrpMet: 0.4 ± 0.015	0.445TrpAsn: 0.445 ± 0.015	0.716TrpPro: 0.716 ± 0.021	0.517TrpGln: 0.517 ± 0.02	1.353TrpArg: 1.353 ± 0.032	0.875TrpSer: 0.875 ± 0.024	0.867TrpThr: 0.867 ± 0.021	0.804TrpVal: 0.804 ± 0.023	0.216TrpTrp: 0.216 ± 0.011	0.298TrpTyr: 0.298 ± 0.014	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.821TyrAla: 2.821 ± 0.039	0.249TyrCys: 0.249 ± 0.011	1.58TyrAsp: 1.58 ± 0.035	1.194TyrGlu: 1.194 ± 0.027	0.869TyrPhe: 0.869 ± 0.022	2.234TyrGly: 2.234 ± 0.036	0.534TyrHis: 0.534 ± 0.019	0.834TyrIle: 0.834 ± 0.019	0.587TyrLys: 0.587 ± 0.019	2.138TyrLeu: 2.138 ± 0.034	0.448TyrMet: 0.448 ± 0.015	0.602TyrAsn: 0.602 ± 0.019	1.016TyrPro: 1.016 ± 0.026	0.723TyrGln: 0.723 ± 0.023	2.106TyrArg: 2.106 ± 0.039	1.19TyrSer: 1.19 ± 0.027	0.983TyrThr: 0.983 ± 0.029	1.712TyrVal: 1.712 ± 0.032	0.348TyrTrp: 0.348 ± 0.013	0.662TyrTyr: 0.662 ± 0.024	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 5313 proteins (1783296 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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