Microbacterium oryzae

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; Microbacterium

Average proteome isoelectric point is 5.99

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2715 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6I6DS87|A0A6I6DS87_9MICO Glycosyl transferase OS=Microbacterium oryzae OX=743009 GN=D7D94_04085 PE=4 SV=1
MM1 pKa = 7.73LLTVLTGCAPQDD13 pKa = 3.64GVAAEE18 pKa = 4.15YY19 pKa = 10.92DD20 pKa = 3.81VGNQTYY26 pKa = 9.92EE27 pKa = 3.98SGDD30 pKa = 3.44FRR32 pKa = 11.84VEE34 pKa = 4.03SIAAEE39 pKa = 4.02EE40 pKa = 4.31RR41 pKa = 11.84GDD43 pKa = 3.54PVVFDD48 pKa = 4.29GVTEE52 pKa = 4.08YY53 pKa = 11.59GDD55 pKa = 3.37AVSDD59 pKa = 3.7VEE61 pKa = 4.28FADD64 pKa = 3.33RR65 pKa = 11.84VYY67 pKa = 11.3VVNFWYY73 pKa = 10.11AACGPCIAEE82 pKa = 3.9APMLEE87 pKa = 4.49EE88 pKa = 4.05VWQKK92 pKa = 10.48YY93 pKa = 6.52QAEE96 pKa = 4.26DD97 pKa = 3.5VGFLGINIYY106 pKa = 9.63DD107 pKa = 3.69QPATARR113 pKa = 11.84AFAADD118 pKa = 3.6NGITYY123 pKa = 9.41PSVIDD128 pKa = 3.74ADD130 pKa = 3.95GGQVKK135 pKa = 9.97LAFAAATPIQATPTTLVMDD154 pKa = 4.3RR155 pKa = 11.84QGRR158 pKa = 11.84VAARR162 pKa = 11.84IIGQLQSASILSTLVSDD179 pKa = 4.86VLAEE183 pKa = 4.49DD184 pKa = 4.13GG185 pKa = 4.26

Molecular weight: 19.68 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6I6DUH6|A0A6I6DUH6_9MICO Aldo/keto reductase OS=Microbacterium oryzae OX=743009 GN=D7D94_00860 PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.87KK16 pKa = 9.7HH17 pKa = 5.84RR18 pKa = 11.84KK19 pKa = 8.56LLRR22 pKa = 11.84KK23 pKa = 7.78TRR25 pKa = 11.84HH26 pKa = 3.65QRR28 pKa = 11.84RR29 pKa = 11.84NKK31 pKa = 9.78KK32 pKa = 9.85

Molecular weight: 4.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2715

0

2715

912640

32

1730

336.1

36.01

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.032 ± 0.07

0.477 ± 0.009

6.373 ± 0.041

6.114 ± 0.045

3.043 ± 0.029

8.93 ± 0.042

2.012 ± 0.02

4.326 ± 0.034

1.804 ± 0.03

10.082 ± 0.059

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.769 ± 0.019

1.758 ± 0.023

5.375 ± 0.033

2.78 ± 0.024

7.744 ± 0.05

5.28 ± 0.03

5.674 ± 0.033

8.982 ± 0.04

1.517 ± 0.017

1.929 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski