Mycobacterium phage Traaww1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Kostyavirus; unclassified Kostyavirus

Average proteome isoelectric point is 6.08

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 142 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A649VHV1|A0A649VHV1_9CAUD Uncharacterized protein OS=Mycobacterium phage Traaww1 OX=2656610 GN=114 PE=4 SV=1
MM1 pKa = 7.27TEE3 pKa = 4.02STKK6 pKa = 10.99GMLTEE11 pKa = 4.13YY12 pKa = 10.73VEE14 pKa = 4.39NAAEE18 pKa = 4.41TIRR21 pKa = 11.84EE22 pKa = 4.02YY23 pKa = 11.36ARR25 pKa = 11.84QIEE28 pKa = 4.33SAEE31 pKa = 3.92YY32 pKa = 9.22GNRR35 pKa = 11.84WTITDD40 pKa = 3.78DD41 pKa = 5.05DD42 pKa = 4.97GNQHH46 pKa = 4.8VTIADD51 pKa = 4.05SEE53 pKa = 4.55SSAYY57 pKa = 10.11EE58 pKa = 3.62IVSEE62 pKa = 4.16NTTALTGEE70 pKa = 4.34PDD72 pKa = 2.94TWVIEE77 pKa = 3.89LDD79 pKa = 3.74EE80 pKa = 4.9FDD82 pKa = 4.41EE83 pKa = 4.24PTIVDD88 pKa = 3.76EE89 pKa = 4.89YY90 pKa = 11.15GNEE93 pKa = 4.11SPISEE98 pKa = 4.22FPISVEE104 pKa = 4.16VKK106 pKa = 9.67IGRR109 pKa = 11.84PLAVVIGTGGPHH121 pKa = 6.97IEE123 pKa = 4.11IVQDD127 pKa = 4.33LSDD130 pKa = 4.58GPAKK134 pKa = 10.51LAGYY138 pKa = 8.34WGSEE142 pKa = 3.39NVYY145 pKa = 10.25RR146 pKa = 11.84YY147 pKa = 10.12GDD149 pKa = 3.54EE150 pKa = 4.12FQTVLDD156 pKa = 4.1YY157 pKa = 10.88LTDD160 pKa = 3.53SLYY163 pKa = 11.27DD164 pKa = 3.64EE165 pKa = 5.96APEE168 pKa = 3.83EE169 pKa = 4.33WKK171 pKa = 10.92

Molecular weight: 19.11 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A649VIM1|A0A649VIM1_9CAUD Endonuclease VII OS=Mycobacterium phage Traaww1 OX=2656610 GN=95 PE=4 SV=1
MM1 pKa = 7.43PRR3 pKa = 11.84SAPKK7 pKa = 10.14PCTKK11 pKa = 10.41CGARR15 pKa = 11.84ANRR18 pKa = 11.84RR19 pKa = 11.84GLCPRR24 pKa = 11.84HH25 pKa = 6.56DD26 pKa = 3.93KK27 pKa = 11.23QGDD30 pKa = 3.69SHH32 pKa = 7.16RR33 pKa = 11.84GSARR37 pKa = 11.84DD38 pKa = 3.15RR39 pKa = 11.84GYY41 pKa = 11.14DD42 pKa = 3.55SEE44 pKa = 5.42HH45 pKa = 5.17EE46 pKa = 4.44TEE48 pKa = 4.79FRR50 pKa = 11.84AAVLAAQPTCPCGQPSTVADD70 pKa = 4.48HH71 pKa = 6.54YY72 pKa = 10.19PLSRR76 pKa = 11.84RR77 pKa = 11.84EE78 pKa = 4.46LIRR81 pKa = 11.84QRR83 pKa = 11.84LNPNDD88 pKa = 3.68PRR90 pKa = 11.84HH91 pKa = 5.63GRR93 pKa = 11.84ALCAACHH100 pKa = 5.17NTHH103 pKa = 5.97TARR106 pKa = 11.84TQSHH110 pKa = 7.03LGRR113 pKa = 11.84RR114 pKa = 11.84SS115 pKa = 2.94

Molecular weight: 12.8 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

142

0

142

23703

32

1577

166.9

18.33

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.294 ± 0.42

1.337 ± 0.169

6.337 ± 0.208

6.691 ± 0.317

2.911 ± 0.152

8.691 ± 0.522

2.578 ± 0.174

4.383 ± 0.143

4.405 ± 0.229

8.096 ± 0.251

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.396 ± 0.115

3.367 ± 0.13

5.286 ± 0.175

3.375 ± 0.202

6.008 ± 0.309

5.535 ± 0.184

6.168 ± 0.262

7.164 ± 0.284

1.962 ± 0.133

3.016 ± 0.141
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski