Cacao virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina; Ellioviricetes; Bunyavirales; Phenuiviridae; Phlebovirus; Cacao phlebovirus

Average proteome isoelectric point is 6.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4P8D7U2|A0A4P8D7U2_9VIRU Glycoprotein OS=Cacao virus OX=629730 PE=4 SV=1
MM1 pKa = 7.58NYY3 pKa = 10.75YY4 pKa = 9.46MLDD7 pKa = 3.6MPLVSYY13 pKa = 8.97STLNIRR19 pKa = 11.84RR20 pKa = 11.84IQVDD24 pKa = 3.44YY25 pKa = 11.66VPFNKK30 pKa = 9.62MSVCPVSKK38 pKa = 10.91YY39 pKa = 10.35EE40 pKa = 4.04DD41 pKa = 3.31MEE43 pKa = 4.71FEE45 pKa = 4.42VTSYY49 pKa = 10.09SQKK52 pKa = 10.23PGSKK56 pKa = 8.26NTLYY60 pKa = 10.89DD61 pKa = 4.49FYY63 pKa = 11.32SVGEE67 pKa = 4.33IPVSWGVNHH76 pKa = 6.91PGVRR80 pKa = 11.84LEE82 pKa = 4.18SSTKK86 pKa = 9.47FLSLIHH92 pKa = 6.45EE93 pKa = 4.66ISRR96 pKa = 11.84LEE98 pKa = 3.86INDD101 pKa = 3.53VLKK104 pKa = 11.12HH105 pKa = 5.77NEE107 pKa = 4.05PNLKK111 pKa = 10.0RR112 pKa = 11.84ALCWPFSYY120 pKa = 8.14PTLQFFSLIRR130 pKa = 11.84LKK132 pKa = 11.11DD133 pKa = 3.55EE134 pKa = 4.83LGPWSYY140 pKa = 11.27KK141 pKa = 10.31GIVMTQIMRR150 pKa = 11.84ASKK153 pKa = 8.87CTQIDD158 pKa = 3.45RR159 pKa = 11.84CVVNFHH165 pKa = 6.76KK166 pKa = 10.72RR167 pKa = 11.84IQRR170 pKa = 11.84EE171 pKa = 3.98ALEE174 pKa = 4.72LGDD177 pKa = 6.49DD178 pKa = 3.76IGCYY182 pKa = 9.45PGRR185 pKa = 11.84NLVLEE190 pKa = 4.56ACSVICLRR198 pKa = 11.84LLQSYY203 pKa = 9.8RR204 pKa = 11.84YY205 pKa = 9.32DD206 pKa = 3.61LEE208 pKa = 5.16YY209 pKa = 10.86DD210 pKa = 3.67APHH213 pKa = 6.45SKK215 pKa = 10.5LMKK218 pKa = 10.19LVQDD222 pKa = 4.0FDD224 pKa = 4.08EE225 pKa = 5.04SFEE228 pKa = 4.29LVSDD232 pKa = 3.78ADD234 pKa = 3.49LRR236 pKa = 11.84MEE238 pKa = 4.23VARR241 pKa = 11.84DD242 pKa = 3.44EE243 pKa = 4.97FDD245 pKa = 3.8TIMKK249 pKa = 10.02RR250 pKa = 11.84RR251 pKa = 11.84EE252 pKa = 3.92AEE254 pKa = 3.89EE255 pKa = 4.36TFWGSDD261 pKa = 3.28MTDD264 pKa = 2.69SDD266 pKa = 3.92

Molecular weight: 31.09 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4P8D7S5|A0A4P8D7S5_9VIRU NSs OS=Cacao virus OX=629730 PE=4 SV=1
MM1 pKa = 7.97SDD3 pKa = 3.59YY4 pKa = 10.9QQLAVEE10 pKa = 5.11FASEE14 pKa = 4.43VPSEE18 pKa = 4.03AVIATWVEE26 pKa = 3.72DD27 pKa = 3.7FAYY30 pKa = 10.38QGFDD34 pKa = 2.79AGEE37 pKa = 4.1VIKK40 pKa = 11.03ALVEE44 pKa = 3.97KK45 pKa = 10.61GKK47 pKa = 10.33NDD49 pKa = 3.22WKK51 pKa = 10.65QDD53 pKa = 3.21ARR55 pKa = 11.84KK56 pKa = 8.99MIILSLTRR64 pKa = 11.84GNKK67 pKa = 7.45PSKK70 pKa = 7.64MTEE73 pKa = 3.73RR74 pKa = 11.84MSDD77 pKa = 3.11AGKK80 pKa = 8.58STVNALVKK88 pKa = 10.26RR89 pKa = 11.84YY90 pKa = 8.35NLKK93 pKa = 10.47SGNPGRR99 pKa = 11.84KK100 pKa = 9.28DD101 pKa = 3.21LTLSRR106 pKa = 11.84IATALAGWTCQAAPIVQDD124 pKa = 3.62YY125 pKa = 11.15LPVTGKK131 pKa = 11.16SMDD134 pKa = 4.11LLSNNYY140 pKa = 8.09PRR142 pKa = 11.84QMMHH146 pKa = 6.72PCFSGLIDD154 pKa = 3.5PTLPKK159 pKa = 9.94NTVDD163 pKa = 3.5NLSFAHH169 pKa = 6.49CLYY172 pKa = 7.95MTQFSKK178 pKa = 9.97TINPSLRR185 pKa = 11.84GLSKK189 pKa = 10.98SEE191 pKa = 4.04VVSSFSQPMNAAINSSFLSSDD212 pKa = 3.01QRR214 pKa = 11.84RR215 pKa = 11.84SFLDD219 pKa = 3.23TLGLLNKK226 pKa = 10.06NLEE229 pKa = 4.44CTSQVIDD236 pKa = 3.39AAKK239 pKa = 10.43AFKK242 pKa = 10.86SLTNN246 pKa = 3.71

Molecular weight: 27.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

3911

246

2090

977.8

110.65

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.5 ± 0.556

3.094 ± 0.662

5.702 ± 0.402

6.52 ± 0.656

4.296 ± 0.287

5.574 ± 0.451

2.199 ± 0.246

7.006 ± 0.579

6.622 ± 0.241

9.0 ± 0.283

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.324 ± 0.191

4.96 ± 0.587

3.554 ± 0.259

3.298 ± 0.15

4.986 ± 0.27

9.946 ± 0.166

5.318 ± 0.206

5.651 ± 0.226

1.176 ± 0.127

3.273 ± 0.272
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski