Lactobacillus virus phiJL1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Coetzeevirus

Average proteome isoelectric point is 6.24

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 46 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q597W9|Q597W9_9CAUD Putative HNH homing endonuclease OS=Lactobacillus virus phiJL1 OX=235201 PE=4 SV=1
MM1 pKa = 7.58SEE3 pKa = 3.8LTIEE7 pKa = 4.13EE8 pKa = 4.34RR9 pKa = 11.84EE10 pKa = 4.01LLKK13 pKa = 10.52WVLQEE18 pKa = 4.27VNSQNSVQPLEE29 pKa = 4.33SLADD33 pKa = 3.51VCEE36 pKa = 4.91SIDD39 pKa = 5.19LGNAPAGLEE48 pKa = 3.78RR49 pKa = 11.84PYY51 pKa = 11.24YY52 pKa = 9.69GMSYY56 pKa = 9.79KK57 pKa = 10.39HH58 pKa = 5.88QLIFLTEE65 pKa = 3.95YY66 pKa = 10.72IMALEE71 pKa = 4.19EE72 pKa = 4.02

Molecular weight: 8.28 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q597W7|Q597W7_9CAUD Putative DNA binding protein OS=Lactobacillus virus phiJL1 OX=235201 PE=4 SV=1
MM1 pKa = 7.57ACLSRR6 pKa = 11.84KK7 pKa = 7.72ATNGSSIISVWAKK20 pKa = 9.72TNSNMADD27 pKa = 3.19NRR29 pKa = 11.84GVGG32 pKa = 3.22

Molecular weight: 3.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

46

0

46

10472

32

1133

227.7

25.21

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.403 ± 1.069

0.497 ± 0.099

6.828 ± 0.425

5.529 ± 0.506

3.657 ± 0.166

6.981 ± 0.49

1.385 ± 0.18

6.694 ± 0.261

7.238 ± 0.354

7.267 ± 0.271

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.702 ± 0.203

6.035 ± 0.364

2.884 ± 0.202

3.571 ± 0.161

3.457 ± 0.353

7.429 ± 0.516

6.885 ± 0.377

7.076 ± 0.25

1.003 ± 0.103

4.479 ± 0.456
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski