Amino acid dipepetide frequency for Mycobacterium phage Melissauren88

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
14.882AlaAla: 14.882 ± 2.074
0.698AlaCys: 0.698 ± 0.174
7.732AlaAsp: 7.732 ± 0.783
7.15AlaGlu: 7.15 ± 0.689
2.732AlaPhe: 2.732 ± 0.466
10.348AlaGly: 10.348 ± 1.539
2.558AlaHis: 2.558 ± 0.432
4.418AlaIle: 4.418 ± 0.529
3.488AlaLys: 3.488 ± 0.485
7.848AlaLeu: 7.848 ± 0.848
2.383AlaMet: 2.383 ± 0.392
2.616AlaAsn: 2.616 ± 0.374
5.348AlaPro: 5.348 ± 0.536
3.314AlaGln: 3.314 ± 0.445
7.208AlaArg: 7.208 ± 0.669
5.29AlaSer: 5.29 ± 0.613
6.453AlaThr: 6.453 ± 0.663
6.743AlaVal: 6.743 ± 0.624
3.023AlaTrp: 3.023 ± 0.65
2.035AlaTyr: 2.035 ± 0.319
0.0AlaXaa: 0.0 ± 0.0
Cys
0.988CysAla: 0.988 ± 0.256
0.0CysCys: 0.0 ± 0.0
1.744CysAsp: 1.744 ± 0.371
0.581CysGlu: 0.581 ± 0.175
0.174CysPhe: 0.174 ± 0.109
1.337CysGly: 1.337 ± 0.276
0.233CysHis: 0.233 ± 0.111
0.233CysIle: 0.233 ± 0.111
0.523CysLys: 0.523 ± 0.169
0.814CysLeu: 0.814 ± 0.281
0.233CysMet: 0.233 ± 0.12
0.291CysAsn: 0.291 ± 0.135
1.279CysPro: 1.279 ± 0.322
0.291CysGln: 0.291 ± 0.117
0.581CysArg: 0.581 ± 0.212
0.698CysSer: 0.698 ± 0.209
0.639CysThr: 0.639 ± 0.184
0.698CysVal: 0.698 ± 0.163
0.349CysTrp: 0.349 ± 0.147
0.174CysTyr: 0.174 ± 0.097
0.0CysXaa: 0.0 ± 0.0
Asp
7.15AspAla: 7.15 ± 0.639
0.756AspCys: 0.756 ± 0.195
4.534AspAsp: 4.534 ± 0.657
3.139AspGlu: 3.139 ± 0.461
2.035AspPhe: 2.035 ± 0.299
7.208AspGly: 7.208 ± 0.706
1.628AspHis: 1.628 ± 0.399
2.5AspIle: 2.5 ± 0.362
1.686AspLys: 1.686 ± 0.299
6.104AspLeu: 6.104 ± 0.636
0.988AspMet: 0.988 ± 0.259
1.57AspAsn: 1.57 ± 0.41
5.232AspPro: 5.232 ± 0.662
2.093AspGln: 2.093 ± 0.322
5.29AspArg: 5.29 ± 0.703
3.72AspSer: 3.72 ± 0.582
4.011AspThr: 4.011 ± 0.503
4.186AspVal: 4.186 ± 0.618
1.744AspTrp: 1.744 ± 0.341
2.035AspTyr: 2.035 ± 0.34
0.0AspXaa: 0.0 ± 0.0
Glu
5.93GluAla: 5.93 ± 0.659
0.93GluCys: 0.93 ± 0.265
3.023GluAsp: 3.023 ± 0.407
2.616GluGlu: 2.616 ± 0.485
2.325GluPhe: 2.325 ± 0.352
2.849GluGly: 2.849 ± 0.381
1.744GluHis: 1.744 ± 0.424
2.267GluIle: 2.267 ± 0.357
1.686GluLys: 1.686 ± 0.246
5.523GluLeu: 5.523 ± 0.65
1.686GluMet: 1.686 ± 0.322
2.151GluAsn: 2.151 ± 0.345
2.849GluPro: 2.849 ± 0.492
2.79GluGln: 2.79 ± 0.432
4.592GluArg: 4.592 ± 0.627
2.616GluSer: 2.616 ± 0.406
4.127GluThr: 4.127 ± 0.665
3.662GluVal: 3.662 ± 0.472
1.279GluTrp: 1.279 ± 0.25
1.977GluTyr: 1.977 ± 0.384
0.0GluXaa: 0.0 ± 0.0
Phe
3.546PheAla: 3.546 ± 0.405
0.349PheCys: 0.349 ± 0.146
2.151PheAsp: 2.151 ± 0.432
1.453PheGlu: 1.453 ± 0.286
0.872PhePhe: 0.872 ± 0.295
2.965PheGly: 2.965 ± 0.684
0.407PheHis: 0.407 ± 0.143
1.744PheIle: 1.744 ± 0.344
1.046PheLys: 1.046 ± 0.219
1.802PheLeu: 1.802 ± 0.224
0.698PheMet: 0.698 ± 0.225
1.163PheAsn: 1.163 ± 0.341
1.511PhePro: 1.511 ± 0.328
1.221PheGln: 1.221 ± 0.297
1.337PheArg: 1.337 ± 0.256
1.511PheSer: 1.511 ± 0.307
2.442PheThr: 2.442 ± 0.379
2.325PheVal: 2.325 ± 0.345
0.581PheTrp: 0.581 ± 0.166
1.046PheTyr: 1.046 ± 0.287
0.0PheXaa: 0.0 ± 0.0
Gly
8.72GlyAla: 8.72 ± 1.108
1.163GlyCys: 1.163 ± 0.293
6.395GlyAsp: 6.395 ± 0.66
3.895GlyGlu: 3.895 ± 0.673
2.965GlyPhe: 2.965 ± 0.479
11.743GlyGly: 11.743 ± 2.742
1.744GlyHis: 1.744 ± 0.297
4.418GlyIle: 4.418 ± 0.628
2.383GlyLys: 2.383 ± 0.393
5.93GlyLeu: 5.93 ± 0.57
2.209GlyMet: 2.209 ± 0.538
3.023GlyAsn: 3.023 ± 0.373
5.058GlyPro: 5.058 ± 0.702
2.383GlyGln: 2.383 ± 0.545
4.999GlyArg: 4.999 ± 0.675
6.278GlySer: 6.278 ± 1.173
6.22GlyThr: 6.22 ± 0.753
5.755GlyVal: 5.755 ± 0.606
2.325GlyTrp: 2.325 ± 0.356
2.209GlyTyr: 2.209 ± 0.343
0.0GlyXaa: 0.0 ± 0.0
His
1.918HisAla: 1.918 ± 0.388
0.233HisCys: 0.233 ± 0.127
1.279HisAsp: 1.279 ± 0.282
1.279HisGlu: 1.279 ± 0.315
0.465HisPhe: 0.465 ± 0.158
2.035HisGly: 2.035 ± 0.364
1.105HisHis: 1.105 ± 0.288
1.453HisIle: 1.453 ± 0.296
0.814HisLys: 0.814 ± 0.274
1.221HisLeu: 1.221 ± 0.304
0.407HisMet: 0.407 ± 0.131
0.756HisAsn: 0.756 ± 0.172
1.744HisPro: 1.744 ± 0.346
0.93HisGln: 0.93 ± 0.22
2.151HisArg: 2.151 ± 0.366
0.756HisSer: 0.756 ± 0.199
1.511HisThr: 1.511 ± 0.353
1.395HisVal: 1.395 ± 0.32
0.523HisTrp: 0.523 ± 0.157
0.756HisTyr: 0.756 ± 0.156
0.0HisXaa: 0.0 ± 0.0
Ile
5.058IleAla: 5.058 ± 0.599
0.639IleCys: 0.639 ± 0.222
4.302IleAsp: 4.302 ± 0.573
3.662IleGlu: 3.662 ± 0.403
0.814IlePhe: 0.814 ± 0.257
3.662IleGly: 3.662 ± 0.528
1.511IleHis: 1.511 ± 0.296
1.221IleIle: 1.221 ± 0.269
1.105IleLys: 1.105 ± 0.258
2.035IleLeu: 2.035 ± 0.412
0.291IleMet: 0.291 ± 0.103
1.977IleAsn: 1.977 ± 0.324
3.314IlePro: 3.314 ± 0.382
1.163IleGln: 1.163 ± 0.227
3.139IleArg: 3.139 ± 0.557
2.442IleSer: 2.442 ± 0.404
3.372IleThr: 3.372 ± 0.446
2.732IleVal: 2.732 ± 0.338
0.988IleTrp: 0.988 ± 0.298
0.698IleTyr: 0.698 ± 0.242
0.0IleXaa: 0.0 ± 0.0
Lys
3.081LysAla: 3.081 ± 0.457
0.639LysCys: 0.639 ± 0.215
1.395LysAsp: 1.395 ± 0.296
1.279LysGlu: 1.279 ± 0.268
1.163LysPhe: 1.163 ± 0.237
2.79LysGly: 2.79 ± 0.31
0.872LysHis: 0.872 ± 0.225
0.93LysIle: 0.93 ± 0.243
1.453LysLys: 1.453 ± 0.421
2.558LysLeu: 2.558 ± 0.516
0.639LysMet: 0.639 ± 0.185
0.756LysAsn: 0.756 ± 0.177
2.442LysPro: 2.442 ± 0.535
1.744LysGln: 1.744 ± 0.245
2.093LysArg: 2.093 ± 0.357
1.977LysSer: 1.977 ± 0.334
1.918LysThr: 1.918 ± 0.361
2.267LysVal: 2.267 ± 0.393
0.698LysTrp: 0.698 ± 0.199
0.988LysTyr: 0.988 ± 0.276
0.0LysXaa: 0.0 ± 0.0
Leu
7.557LeuAla: 7.557 ± 0.87
0.698LeuCys: 0.698 ± 0.232
5.058LeuAsp: 5.058 ± 0.693
3.837LeuGlu: 3.837 ± 0.493
2.616LeuPhe: 2.616 ± 0.31
5.058LeuGly: 5.058 ± 0.501
0.814LeuHis: 0.814 ± 0.196
3.314LeuIle: 3.314 ± 0.469
1.744LeuLys: 1.744 ± 0.313
4.709LeuLeu: 4.709 ± 0.547
1.628LeuMet: 1.628 ± 0.336
2.674LeuAsn: 2.674 ± 0.376
4.941LeuPro: 4.941 ± 0.693
2.558LeuGln: 2.558 ± 0.392
5.406LeuArg: 5.406 ± 0.72
5.464LeuSer: 5.464 ± 0.551
5.523LeuThr: 5.523 ± 0.559
5.058LeuVal: 5.058 ± 0.533
1.337LeuTrp: 1.337 ± 0.315
2.093LeuTyr: 2.093 ± 0.356
0.0LeuXaa: 0.0 ± 0.0
Met
2.442MetAla: 2.442 ± 0.424
0.174MetCys: 0.174 ± 0.112
1.279MetAsp: 1.279 ± 0.247
0.698MetGlu: 0.698 ± 0.162
0.639MetPhe: 0.639 ± 0.185
1.686MetGly: 1.686 ± 0.322
0.291MetHis: 0.291 ± 0.122
0.93MetIle: 0.93 ± 0.247
0.93MetLys: 0.93 ± 0.232
1.802MetLeu: 1.802 ± 0.281
0.581MetMet: 0.581 ± 0.221
1.046MetAsn: 1.046 ± 0.225
1.57MetPro: 1.57 ± 0.329
0.639MetGln: 0.639 ± 0.15
1.453MetArg: 1.453 ± 0.318
2.79MetSer: 2.79 ± 0.441
2.151MetThr: 2.151 ± 0.353
1.453MetVal: 1.453 ± 0.346
0.291MetTrp: 0.291 ± 0.123
0.291MetTyr: 0.291 ± 0.118
0.0MetXaa: 0.0 ± 0.0
Asn
3.779AsnAla: 3.779 ± 0.414
0.233AsnCys: 0.233 ± 0.115
1.628AsnAsp: 1.628 ± 0.335
1.628AsnGlu: 1.628 ± 0.29
0.814AsnPhe: 0.814 ± 0.295
4.069AsnGly: 4.069 ± 0.663
0.872AsnHis: 0.872 ± 0.202
1.686AsnIle: 1.686 ± 0.431
1.046AsnLys: 1.046 ± 0.294
2.151AsnLeu: 2.151 ± 0.376
0.523AsnMet: 0.523 ± 0.148
1.977AsnAsn: 1.977 ± 0.346
2.558AsnPro: 2.558 ± 0.373
1.163AsnGln: 1.163 ± 0.31
2.267AsnArg: 2.267 ± 0.389
1.511AsnSer: 1.511 ± 0.323
2.267AsnThr: 2.267 ± 0.38
2.093AsnVal: 2.093 ± 0.374
0.872AsnTrp: 0.872 ± 0.167
0.523AsnTyr: 0.523 ± 0.148
0.0AsnXaa: 0.0 ± 0.0
Pro
5.464ProAla: 5.464 ± 0.642
0.814ProCys: 0.814 ± 0.239
4.767ProAsp: 4.767 ± 0.651
4.127ProGlu: 4.127 ± 0.444
1.918ProPhe: 1.918 ± 0.342
6.685ProGly: 6.685 ± 0.624
1.686ProHis: 1.686 ± 0.368
2.558ProIle: 2.558 ± 0.291
2.383ProLys: 2.383 ± 0.468
4.534ProLeu: 4.534 ± 0.554
1.977ProMet: 1.977 ± 0.392
2.383ProAsn: 2.383 ± 0.383
3.837ProPro: 3.837 ± 0.663
2.5ProGln: 2.5 ± 0.428
3.662ProArg: 3.662 ± 0.574
3.081ProSer: 3.081 ± 0.471
3.139ProThr: 3.139 ± 0.448
4.767ProVal: 4.767 ± 0.593
1.046ProTrp: 1.046 ± 0.231
1.744ProTyr: 1.744 ± 0.267
0.0ProXaa: 0.0 ± 0.0
Gln
4.941GlnAla: 4.941 ± 0.647
0.291GlnCys: 0.291 ± 0.13
1.744GlnAsp: 1.744 ± 0.275
1.744GlnGlu: 1.744 ± 0.344
1.163GlnPhe: 1.163 ± 0.231
2.151GlnGly: 2.151 ± 0.551
0.814GlnHis: 0.814 ± 0.239
1.686GlnIle: 1.686 ± 0.307
1.105GlnLys: 1.105 ± 0.233
3.139GlnLeu: 3.139 ± 0.368
0.407GlnMet: 0.407 ± 0.165
0.872GlnAsn: 0.872 ± 0.255
2.907GlnPro: 2.907 ± 0.464
1.105GlnGln: 1.105 ± 0.262
2.383GlnArg: 2.383 ± 0.362
2.558GlnSer: 2.558 ± 0.354
1.686GlnThr: 1.686 ± 0.315
2.325GlnVal: 2.325 ± 0.373
0.639GlnTrp: 0.639 ± 0.215
0.988GlnTyr: 0.988 ± 0.298
0.0GlnXaa: 0.0 ± 0.0
Arg
6.104ArgAla: 6.104 ± 0.601
1.221ArgCys: 1.221 ± 0.33
4.011ArgAsp: 4.011 ± 0.52
4.825ArgGlu: 4.825 ± 0.724
2.267ArgPhe: 2.267 ± 0.41
4.011ArgGly: 4.011 ± 0.483
1.221ArgHis: 1.221 ± 0.295
3.895ArgIle: 3.895 ± 0.56
2.209ArgLys: 2.209 ± 0.444
4.767ArgLeu: 4.767 ± 0.538
2.558ArgMet: 2.558 ± 0.391
2.383ArgAsn: 2.383 ± 0.511
4.011ArgPro: 4.011 ± 0.504
2.209ArgGln: 2.209 ± 0.344
5.813ArgArg: 5.813 ± 0.801
3.546ArgSer: 3.546 ± 0.414
3.255ArgThr: 3.255 ± 0.456
4.534ArgVal: 4.534 ± 0.59
2.209ArgTrp: 2.209 ± 0.348
1.918ArgTyr: 1.918 ± 0.308
0.0ArgXaa: 0.0 ± 0.0
Ser
6.86SerAla: 6.86 ± 1.395
0.639SerCys: 0.639 ± 0.213
4.127SerAsp: 4.127 ± 0.524
3.314SerGlu: 3.314 ± 0.439
2.035SerPhe: 2.035 ± 0.432
6.336SerGly: 6.336 ± 0.923
1.279SerHis: 1.279 ± 0.271
2.79SerIle: 2.79 ± 0.468
2.558SerLys: 2.558 ± 0.453
3.779SerLeu: 3.779 ± 0.572
1.453SerMet: 1.453 ± 0.263
2.093SerAsn: 2.093 ± 0.378
3.023SerPro: 3.023 ± 0.341
1.86SerGln: 1.86 ± 0.332
3.255SerArg: 3.255 ± 0.432
3.779SerSer: 3.779 ± 0.772
3.197SerThr: 3.197 ± 0.511
4.709SerVal: 4.709 ± 0.512
1.57SerTrp: 1.57 ± 0.293
1.337SerTyr: 1.337 ± 0.227
0.0SerXaa: 0.0 ± 0.0
Thr
6.395ThrAla: 6.395 ± 0.696
0.698ThrCys: 0.698 ± 0.248
4.069ThrAsp: 4.069 ± 0.592
3.546ThrGlu: 3.546 ± 0.426
1.802ThrPhe: 1.802 ± 0.358
5.93ThrGly: 5.93 ± 0.654
1.57ThrHis: 1.57 ± 0.321
3.372ThrIle: 3.372 ± 0.454
2.093ThrLys: 2.093 ± 0.308
4.709ThrLeu: 4.709 ± 0.532
1.511ThrMet: 1.511 ± 0.307
2.267ThrAsn: 2.267 ± 0.428
4.534ThrPro: 4.534 ± 0.581
2.151ThrGln: 2.151 ± 0.34
3.255ThrArg: 3.255 ± 0.358
4.011ThrSer: 4.011 ± 0.56
4.534ThrThr: 4.534 ± 0.653
5.871ThrVal: 5.871 ± 0.648
1.105ThrTrp: 1.105 ± 0.279
1.802ThrTyr: 1.802 ± 0.277
0.0ThrXaa: 0.0 ± 0.0
Val
6.86ValAla: 6.86 ± 0.547
1.046ValCys: 1.046 ± 0.244
5.232ValAsp: 5.232 ± 0.677
4.767ValGlu: 4.767 ± 0.684
1.86ValPhe: 1.86 ± 0.346
5.813ValGly: 5.813 ± 0.641
1.337ValHis: 1.337 ± 0.303
2.674ValIle: 2.674 ± 0.458
2.035ValLys: 2.035 ± 0.391
4.883ValLeu: 4.883 ± 0.653
1.86ValMet: 1.86 ± 0.31
2.151ValAsn: 2.151 ± 0.358
4.069ValPro: 4.069 ± 0.386
2.849ValGln: 2.849 ± 0.339
4.127ValArg: 4.127 ± 0.58
5.174ValSer: 5.174 ± 0.604
4.941ValThr: 4.941 ± 0.489
6.22ValVal: 6.22 ± 0.867
1.86ValTrp: 1.86 ± 0.382
1.453ValTyr: 1.453 ± 0.266
0.0ValXaa: 0.0 ± 0.0
Trp
1.918TrpAla: 1.918 ± 0.273
0.233TrpCys: 0.233 ± 0.11
1.511TrpAsp: 1.511 ± 0.277
1.279TrpGlu: 1.279 ± 0.323
0.872TrpPhe: 0.872 ± 0.223
1.279TrpGly: 1.279 ± 0.33
0.581TrpHis: 0.581 ± 0.188
1.046TrpIle: 1.046 ± 0.211
0.698TrpLys: 0.698 ± 0.182
1.57TrpLeu: 1.57 ± 0.323
0.872TrpMet: 0.872 ± 0.284
0.639TrpAsn: 0.639 ± 0.238
1.279TrpPro: 1.279 ± 0.334
1.046TrpGln: 1.046 ± 0.306
2.267TrpArg: 2.267 ± 0.447
1.918TrpSer: 1.918 ± 0.592
1.86TrpThr: 1.86 ± 0.35
1.918TrpVal: 1.918 ± 0.418
1.046TrpTrp: 1.046 ± 0.214
0.291TrpTyr: 0.291 ± 0.127
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.674TyrAla: 2.674 ± 0.424
0.407TyrCys: 0.407 ± 0.151
1.628TyrAsp: 1.628 ± 0.375
1.802TyrGlu: 1.802 ± 0.354
0.639TyrPhe: 0.639 ± 0.173
1.86TyrGly: 1.86 ± 0.343
0.465TyrHis: 0.465 ± 0.134
0.988TyrIle: 0.988 ± 0.204
0.698TyrLys: 0.698 ± 0.186
2.209TyrLeu: 2.209 ± 0.36
0.174TyrMet: 0.174 ± 0.09
0.872TyrAsn: 0.872 ± 0.235
1.57TyrPro: 1.57 ± 0.212
0.698TyrGln: 0.698 ± 0.21
1.686TyrArg: 1.686 ± 0.302
0.872TyrSer: 0.872 ± 0.202
1.977TyrThr: 1.977 ± 0.405
2.5TyrVal: 2.5 ± 0.359
0.639TyrTrp: 0.639 ± 0.193
0.639TyrTyr: 0.639 ± 0.169
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 95 proteins (17203 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski