Apis mellifera associated microvirus 4

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 5.88

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3Q8U5M0|A0A3Q8U5M0_9VIRU Major capsid protein OS=Apis mellifera associated microvirus 4 OX=2494769 PE=3 SV=1
MM1 pKa = 7.73RR2 pKa = 11.84VQLFSVFDD10 pKa = 3.79TKK12 pKa = 11.27SRR14 pKa = 11.84IYY16 pKa = 10.31LAPFVARR23 pKa = 11.84SQVDD27 pKa = 3.26ATRR30 pKa = 11.84QIASSFRR37 pKa = 11.84DD38 pKa = 3.61PQMKK42 pKa = 8.37DD43 pKa = 2.91TPVGQNPEE51 pKa = 4.18DD52 pKa = 3.9FEE54 pKa = 5.0LFLVGSFDD62 pKa = 5.03DD63 pKa = 4.17EE64 pKa = 4.88SGDD67 pKa = 3.58MSVCKK72 pKa = 8.92PTFVANLGNLRR83 pKa = 11.84DD84 pKa = 4.09DD85 pKa = 3.99SRR87 pKa = 11.84GSTVSSS93 pKa = 3.56

Molecular weight: 10.33 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3Q8U5P7|A0A3Q8U5P7_9VIRU Uncharacterized protein OS=Apis mellifera associated microvirus 4 OX=2494769 PE=4 SV=1
MM1 pKa = 7.46AALMCSATWVHH12 pKa = 6.14PTHH15 pKa = 6.78GVTKK19 pKa = 10.28CGQCMEE25 pKa = 4.47CRR27 pKa = 11.84LAYY30 pKa = 9.99SRR32 pKa = 11.84EE33 pKa = 3.38WAIRR37 pKa = 11.84ITHH40 pKa = 6.32EE41 pKa = 4.56AQMHH45 pKa = 5.73DD46 pKa = 3.96HH47 pKa = 6.65NCFLTLTYY55 pKa = 11.22DD56 pKa = 3.6NDD58 pKa = 3.56NLPLHH63 pKa = 5.95GQLVKK68 pKa = 10.61KK69 pKa = 10.41DD70 pKa = 3.47LQDD73 pKa = 3.16FFKK76 pKa = 10.75RR77 pKa = 11.84LRR79 pKa = 11.84HH80 pKa = 4.0VTGPFRR86 pKa = 11.84YY87 pKa = 9.17VASGEE92 pKa = 3.97YY93 pKa = 10.78GEE95 pKa = 4.81LKK97 pKa = 10.18RR98 pKa = 11.84RR99 pKa = 11.84PHH101 pKa = 5.28FHH103 pKa = 5.77VALFGVDD110 pKa = 4.07FHH112 pKa = 7.31EE113 pKa = 5.14DD114 pKa = 3.54RR115 pKa = 11.84IDD117 pKa = 3.6FGAGIRR123 pKa = 11.84GDD125 pKa = 3.55KK126 pKa = 10.45TFVSPTVASVWQKK139 pKa = 10.7SVFPMGHH146 pKa = 6.57TIGSLTFEE154 pKa = 4.08SAAYY158 pKa = 7.99IARR161 pKa = 11.84YY162 pKa = 6.79ITKK165 pKa = 10.06KK166 pKa = 9.24VTGKK170 pKa = 10.16NICPVPLWSDD180 pKa = 3.33PDD182 pKa = 3.66TGEE185 pKa = 3.89MVLPNPEE192 pKa = 4.2FLVCSRR198 pKa = 11.84GIGKK202 pKa = 9.47AWFRR206 pKa = 11.84DD207 pKa = 3.66YY208 pKa = 11.39FMSDD212 pKa = 3.17VFPHH216 pKa = 6.09ARR218 pKa = 11.84VITAQGSPAPVPRR231 pKa = 11.84YY232 pKa = 9.6YY233 pKa = 11.07KK234 pKa = 10.71NLLKK238 pKa = 10.7EE239 pKa = 4.12VGADD243 pKa = 3.18LAMEE247 pKa = 4.25MSYY250 pKa = 10.38RR251 pKa = 11.84ASVNMDD257 pKa = 3.26RR258 pKa = 11.84DD259 pKa = 3.33LDD261 pKa = 3.78RR262 pKa = 11.84RR263 pKa = 11.84KK264 pKa = 10.47VEE266 pKa = 4.31DD267 pKa = 3.64LPSRR271 pKa = 11.84RR272 pKa = 11.84SARR275 pKa = 11.84AVYY278 pKa = 10.03AKK280 pKa = 10.7ARR282 pKa = 11.84TGLFKK287 pKa = 10.62RR288 pKa = 11.84DD289 pKa = 3.29VKK291 pKa = 10.99EE292 pKa = 3.7II293 pKa = 3.7

Molecular weight: 33.39 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1412

93

532

235.3

25.87

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.773 ± 1.395

1.062 ± 0.492

5.878 ± 0.939

4.391 ± 0.392

5.595 ± 0.736

8.357 ± 1.39

1.841 ± 0.68

4.391 ± 0.78

3.399 ± 0.846

8.003 ± 0.281

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.187 ± 0.27

3.824 ± 0.623

5.241 ± 0.705

4.816 ± 1.023

6.586 ± 0.611

6.87 ± 1.024

5.878 ± 1.017

7.153 ± 0.669

0.992 ± 0.287

2.762 ± 0.547
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski