Amino acid dipepetide frequency for Caulobacter phage RW

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
15.332AlaAla: 15.332 ± 1.086
1.04AlaCys: 1.04 ± 0.277
8.816AlaAsp: 8.816 ± 1.392
7.556AlaGlu: 7.556 ± 0.808
3.778AlaPhe: 3.778 ± 0.49
8.542AlaGly: 8.542 ± 0.903
2.026AlaHis: 2.026 ± 0.325
4.654AlaIle: 4.654 ± 0.491
5.804AlaLys: 5.804 ± 0.654
13.579AlaLeu: 13.579 ± 0.802
4.216AlaMet: 4.216 ± 0.498
4.545AlaAsn: 4.545 ± 1.304
5.421AlaPro: 5.421 ± 0.588
5.147AlaGln: 5.147 ± 0.489
7.666AlaArg: 7.666 ± 0.644
5.749AlaSer: 5.749 ± 0.712
6.68AlaThr: 6.68 ± 0.739
7.721AlaVal: 7.721 ± 0.705
2.245AlaTrp: 2.245 ± 0.399
3.45AlaTyr: 3.45 ± 0.434
0.0AlaXaa: 0.0 ± 0.0
Cys
1.259CysAla: 1.259 ± 0.33
0.11CysCys: 0.11 ± 0.074
0.657CysAsp: 0.657 ± 0.211
0.548CysGlu: 0.548 ± 0.182
0.219CysPhe: 0.219 ± 0.121
1.095CysGly: 1.095 ± 0.305
0.164CysHis: 0.164 ± 0.098
0.548CysIle: 0.548 ± 0.158
0.602CysLys: 0.602 ± 0.211
0.383CysLeu: 0.383 ± 0.165
0.329CysMet: 0.329 ± 0.126
0.329CysAsn: 0.329 ± 0.121
0.712CysPro: 0.712 ± 0.267
0.274CysGln: 0.274 ± 0.119
0.548CysArg: 0.548 ± 0.154
0.383CysSer: 0.383 ± 0.15
0.383CysThr: 0.383 ± 0.134
0.493CysVal: 0.493 ± 0.159
0.383CysTrp: 0.383 ± 0.178
0.329CysTyr: 0.329 ± 0.114
0.0CysXaa: 0.0 ± 0.0
Asp
7.392AspAla: 7.392 ± 0.718
0.602AspCys: 0.602 ± 0.169
4.216AspAsp: 4.216 ± 0.754
4.983AspGlu: 4.983 ± 0.531
2.026AspPhe: 2.026 ± 0.301
5.311AspGly: 5.311 ± 0.704
1.095AspHis: 1.095 ± 0.217
2.19AspIle: 2.19 ± 0.392
2.519AspLys: 2.519 ± 0.423
6.516AspLeu: 6.516 ± 1.398
1.588AspMet: 1.588 ± 0.248
1.643AspAsn: 1.643 ± 0.302
4.107AspPro: 4.107 ± 0.559
1.862AspGln: 1.862 ± 0.272
4.654AspArg: 4.654 ± 0.583
2.683AspSer: 2.683 ± 0.35
3.121AspThr: 3.121 ± 0.342
3.559AspVal: 3.559 ± 0.361
1.259AspTrp: 1.259 ± 0.266
1.752AspTyr: 1.752 ± 0.353
0.0AspXaa: 0.0 ± 0.0
Glu
9.254GluAla: 9.254 ± 0.991
0.383GluCys: 0.383 ± 0.157
3.395GluAsp: 3.395 ± 0.451
4.435GluGlu: 4.435 ± 0.551
2.026GluPhe: 2.026 ± 0.351
3.888GluGly: 3.888 ± 0.471
1.424GluHis: 1.424 ± 0.276
2.574GluIle: 2.574 ± 0.318
2.574GluLys: 2.574 ± 0.399
5.311GluLeu: 5.311 ± 0.679
1.424GluMet: 1.424 ± 0.287
1.752GluAsn: 1.752 ± 0.277
2.847GluPro: 2.847 ± 0.444
2.574GluGln: 2.574 ± 0.492
4.654GluArg: 4.654 ± 0.51
1.697GluSer: 1.697 ± 0.273
3.833GluThr: 3.833 ± 0.437
3.614GluVal: 3.614 ± 0.507
1.369GluTrp: 1.369 ± 0.285
1.424GluTyr: 1.424 ± 0.237
0.0GluXaa: 0.0 ± 0.0
Phe
3.45PheAla: 3.45 ± 0.494
0.329PheCys: 0.329 ± 0.114
2.409PheAsp: 2.409 ± 0.352
1.752PheGlu: 1.752 ± 0.287
1.15PhePhe: 1.15 ± 0.217
2.574PheGly: 2.574 ± 0.267
0.438PheHis: 0.438 ± 0.185
0.986PheIle: 0.986 ± 0.222
1.533PheLys: 1.533 ± 0.301
1.697PheLeu: 1.697 ± 0.209
0.438PheMet: 0.438 ± 0.138
1.15PheAsn: 1.15 ± 0.268
1.095PhePro: 1.095 ± 0.263
0.986PheGln: 0.986 ± 0.216
1.697PheArg: 1.697 ± 0.274
2.245PheSer: 2.245 ± 0.277
2.847PheThr: 2.847 ± 0.516
2.464PheVal: 2.464 ± 0.387
0.329PheTrp: 0.329 ± 0.153
1.314PheTyr: 1.314 ± 0.318
0.0PheXaa: 0.0 ± 0.0
Gly
11.718GlyAla: 11.718 ± 2.02
0.767GlyCys: 0.767 ± 0.225
5.749GlyAsp: 5.749 ± 0.758
5.038GlyGlu: 5.038 ± 0.576
2.245GlyPhe: 2.245 ± 0.353
6.735GlyGly: 6.735 ± 0.928
1.095GlyHis: 1.095 ± 0.327
2.081GlyIle: 2.081 ± 0.345
3.395GlyLys: 3.395 ± 0.533
6.023GlyLeu: 6.023 ± 0.599
2.026GlyMet: 2.026 ± 0.303
1.862GlyAsn: 1.862 ± 0.295
2.793GlyPro: 2.793 ± 0.318
3.231GlyGln: 3.231 ± 0.537
4.928GlyArg: 4.928 ± 0.661
4.764GlySer: 4.764 ± 0.774
5.092GlyThr: 5.092 ± 0.87
6.735GlyVal: 6.735 ± 0.838
1.15GlyTrp: 1.15 ± 0.243
2.464GlyTyr: 2.464 ± 0.359
0.0GlyXaa: 0.0 ± 0.0
His
1.971HisAla: 1.971 ± 0.395
0.164HisCys: 0.164 ± 0.098
0.986HisAsp: 0.986 ± 0.29
0.876HisGlu: 0.876 ± 0.192
0.493HisPhe: 0.493 ± 0.166
1.752HisGly: 1.752 ± 0.429
0.548HisHis: 0.548 ± 0.217
1.095HisIle: 1.095 ± 0.219
0.438HisLys: 0.438 ± 0.153
1.643HisLeu: 1.643 ± 0.328
0.383HisMet: 0.383 ± 0.166
0.712HisAsn: 0.712 ± 0.214
1.314HisPro: 1.314 ± 0.265
0.602HisGln: 0.602 ± 0.179
1.588HisArg: 1.588 ± 0.286
0.876HisSer: 0.876 ± 0.266
0.712HisThr: 0.712 ± 0.217
1.259HisVal: 1.259 ± 0.217
0.329HisTrp: 0.329 ± 0.16
0.438HisTyr: 0.438 ± 0.144
0.0HisXaa: 0.0 ± 0.0
Ile
5.366IleAla: 5.366 ± 0.626
0.438IleCys: 0.438 ± 0.131
3.176IleAsp: 3.176 ± 0.368
3.176IleGlu: 3.176 ± 0.424
0.767IlePhe: 0.767 ± 0.182
3.285IleGly: 3.285 ± 0.435
0.821IleHis: 0.821 ± 0.212
1.862IleIle: 1.862 ± 0.315
1.916IleLys: 1.916 ± 0.387
2.519IleLeu: 2.519 ± 0.368
0.931IleMet: 0.931 ± 0.217
0.931IleAsn: 0.931 ± 0.293
1.971IlePro: 1.971 ± 0.371
1.04IleGln: 1.04 ± 0.266
3.176IleArg: 3.176 ± 0.407
1.533IleSer: 1.533 ± 0.303
2.081IleThr: 2.081 ± 0.326
2.793IleVal: 2.793 ± 0.437
0.219IleTrp: 0.219 ± 0.103
0.876IleTyr: 0.876 ± 0.221
0.0IleXaa: 0.0 ± 0.0
Lys
5.038LysAla: 5.038 ± 0.816
0.274LysCys: 0.274 ± 0.134
3.066LysAsp: 3.066 ± 0.427
2.683LysGlu: 2.683 ± 0.422
0.657LysPhe: 0.657 ± 0.2
2.683LysGly: 2.683 ± 0.397
0.986LysHis: 0.986 ± 0.251
1.533LysIle: 1.533 ± 0.289
2.354LysLys: 2.354 ± 0.382
3.942LysLeu: 3.942 ± 0.42
0.821LysMet: 0.821 ± 0.179
0.931LysAsn: 0.931 ± 0.23
3.559LysPro: 3.559 ± 0.529
1.533LysGln: 1.533 ± 0.278
3.285LysArg: 3.285 ± 0.471
2.026LysSer: 2.026 ± 0.303
3.012LysThr: 3.012 ± 0.481
2.464LysVal: 2.464 ± 0.402
0.767LysTrp: 0.767 ± 0.2
1.04LysTyr: 1.04 ± 0.274
0.0LysXaa: 0.0 ± 0.0
Leu
10.623LeuAla: 10.623 ± 0.788
1.095LeuCys: 1.095 ± 0.321
5.202LeuAsp: 5.202 ± 0.529
5.202LeuGlu: 5.202 ± 0.565
2.847LeuPhe: 2.847 ± 0.446
6.406LeuGly: 6.406 ± 0.729
1.369LeuHis: 1.369 ± 0.284
3.888LeuIle: 3.888 ± 0.526
3.778LeuLys: 3.778 ± 0.504
6.625LeuLeu: 6.625 ± 0.528
1.478LeuMet: 1.478 ± 0.307
3.066LeuAsn: 3.066 ± 0.383
5.038LeuPro: 5.038 ± 0.576
2.409LeuGln: 2.409 ± 0.414
5.968LeuArg: 5.968 ± 0.718
7.228LeuSer: 7.228 ± 1.911
5.859LeuThr: 5.859 ± 0.531
4.545LeuVal: 4.545 ± 0.551
1.369LeuTrp: 1.369 ± 0.341
1.971LeuTyr: 1.971 ± 0.354
0.0LeuXaa: 0.0 ± 0.0
Met
2.793MetAla: 2.793 ± 0.457
0.055MetCys: 0.055 ± 0.059
0.767MetAsp: 0.767 ± 0.222
1.095MetGlu: 1.095 ± 0.27
0.931MetPhe: 0.931 ± 0.243
1.752MetGly: 1.752 ± 0.295
0.219MetHis: 0.219 ± 0.138
1.369MetIle: 1.369 ± 0.24
0.383MetLys: 0.383 ± 0.136
1.697MetLeu: 1.697 ± 0.279
0.329MetMet: 0.329 ± 0.102
0.767MetAsn: 0.767 ± 0.185
0.931MetPro: 0.931 ± 0.216
0.986MetGln: 0.986 ± 0.238
2.409MetArg: 2.409 ± 0.391
1.588MetSer: 1.588 ± 0.297
2.519MetThr: 2.519 ± 0.389
1.643MetVal: 1.643 ± 0.295
0.274MetTrp: 0.274 ± 0.114
0.329MetTyr: 0.329 ± 0.117
0.0MetXaa: 0.0 ± 0.0
Asn
4.161AsnAla: 4.161 ± 0.409
0.274AsnCys: 0.274 ± 0.141
1.752AsnAsp: 1.752 ± 0.299
1.314AsnGlu: 1.314 ± 0.247
0.876AsnPhe: 0.876 ± 0.243
2.628AsnGly: 2.628 ± 0.422
0.329AsnHis: 0.329 ± 0.124
1.314AsnIle: 1.314 ± 0.255
1.314AsnLys: 1.314 ± 0.244
3.34AsnLeu: 3.34 ± 1.291
0.493AsnMet: 0.493 ± 0.157
1.095AsnAsn: 1.095 ± 0.244
1.971AsnPro: 1.971 ± 0.307
0.931AsnGln: 0.931 ± 0.193
1.862AsnArg: 1.862 ± 0.216
2.081AsnSer: 2.081 ± 0.311
2.081AsnThr: 2.081 ± 0.384
1.971AsnVal: 1.971 ± 0.359
0.876AsnTrp: 0.876 ± 0.259
1.04AsnTyr: 1.04 ± 0.223
0.0AsnXaa: 0.0 ± 0.0
Pro
4.49ProAla: 4.49 ± 0.642
0.329ProCys: 0.329 ± 0.137
3.45ProAsp: 3.45 ± 0.367
3.285ProGlu: 3.285 ± 0.476
1.478ProPhe: 1.478 ± 0.304
4.326ProGly: 4.326 ± 0.493
1.259ProHis: 1.259 ± 0.286
1.697ProIle: 1.697 ± 0.275
2.574ProLys: 2.574 ± 0.551
3.723ProLeu: 3.723 ± 0.443
1.04ProMet: 1.04 ± 0.259
1.752ProAsn: 1.752 ± 0.329
3.614ProPro: 3.614 ± 0.437
2.409ProGln: 2.409 ± 0.327
3.395ProArg: 3.395 ± 0.44
3.559ProSer: 3.559 ± 0.462
4.326ProThr: 4.326 ± 0.496
4.216ProVal: 4.216 ± 0.557
0.986ProTrp: 0.986 ± 0.235
1.259ProTyr: 1.259 ± 0.249
0.0ProXaa: 0.0 ± 0.0
Gln
5.257GlnAla: 5.257 ± 0.559
0.329GlnCys: 0.329 ± 0.114
1.643GlnAsp: 1.643 ± 0.367
2.026GlnGlu: 2.026 ± 0.312
1.15GlnPhe: 1.15 ± 0.216
3.231GlnGly: 3.231 ± 0.414
0.767GlnHis: 0.767 ± 0.209
0.821GlnIle: 0.821 ± 0.198
0.876GlnLys: 0.876 ± 0.315
2.902GlnLeu: 2.902 ± 0.535
1.15GlnMet: 1.15 ± 0.285
1.095GlnAsn: 1.095 ± 0.249
2.574GlnPro: 2.574 ± 0.363
2.245GlnGln: 2.245 ± 0.588
2.738GlnArg: 2.738 ± 0.418
2.081GlnSer: 2.081 ± 0.298
2.738GlnThr: 2.738 ± 0.379
1.862GlnVal: 1.862 ± 0.314
0.657GlnTrp: 0.657 ± 0.171
1.15GlnTyr: 1.15 ± 0.22
0.0GlnXaa: 0.0 ± 0.0
Arg
8.432ArgAla: 8.432 ± 0.885
1.259ArgCys: 1.259 ± 0.27
4.654ArgAsp: 4.654 ± 0.594
4.107ArgGlu: 4.107 ± 0.466
2.026ArgPhe: 2.026 ± 0.457
3.888ArgGly: 3.888 ± 0.652
1.478ArgHis: 1.478 ± 0.27
3.285ArgIle: 3.285 ± 0.376
4.435ArgLys: 4.435 ± 0.551
6.242ArgLeu: 6.242 ± 0.765
1.643ArgMet: 1.643 ± 0.304
2.683ArgAsn: 2.683 ± 0.412
2.574ArgPro: 2.574 ± 0.414
2.19ArgGln: 2.19 ± 0.437
4.107ArgArg: 4.107 ± 0.547
3.45ArgSer: 3.45 ± 0.711
3.285ArgThr: 3.285 ± 0.441
5.092ArgVal: 5.092 ± 0.576
1.15ArgTrp: 1.15 ± 0.2
2.464ArgTyr: 2.464 ± 0.392
0.0ArgXaa: 0.0 ± 0.0
Ser
7.721SerAla: 7.721 ± 1.024
0.274SerCys: 0.274 ± 0.114
3.833SerAsp: 3.833 ± 0.38
2.847SerGlu: 2.847 ± 0.336
1.862SerPhe: 1.862 ± 0.296
7.228SerGly: 7.228 ± 1.893
1.095SerHis: 1.095 ± 0.274
1.971SerIle: 1.971 ± 0.271
1.533SerLys: 1.533 ± 0.303
4.38SerLeu: 4.38 ± 0.503
1.04SerMet: 1.04 ± 0.274
1.862SerAsn: 1.862 ± 0.334
2.793SerPro: 2.793 ± 0.424
1.916SerGln: 1.916 ± 0.32
3.285SerArg: 3.285 ± 0.364
2.354SerSer: 2.354 ± 0.457
4.271SerThr: 4.271 ± 0.684
3.778SerVal: 3.778 ± 0.475
0.821SerTrp: 0.821 ± 0.196
0.931SerTyr: 0.931 ± 0.198
0.0SerXaa: 0.0 ± 0.0
Thr
7.009ThrAla: 7.009 ± 0.999
0.438ThrCys: 0.438 ± 0.155
3.066ThrAsp: 3.066 ± 0.394
2.574ThrGlu: 2.574 ± 0.368
2.683ThrPhe: 2.683 ± 0.392
6.187ThrGly: 6.187 ± 0.884
0.931ThrHis: 0.931 ± 0.24
2.847ThrIle: 2.847 ± 0.564
2.245ThrLys: 2.245 ± 0.349
6.461ThrLeu: 6.461 ± 0.806
0.821ThrMet: 0.821 ± 0.202
2.409ThrAsn: 2.409 ± 0.366
4.271ThrPro: 4.271 ± 0.456
2.628ThrGln: 2.628 ± 0.438
3.121ThrArg: 3.121 ± 0.498
3.997ThrSer: 3.997 ± 0.672
4.599ThrThr: 4.599 ± 0.924
5.64ThrVal: 5.64 ± 0.711
0.986ThrTrp: 0.986 ± 0.236
1.643ThrTyr: 1.643 ± 0.268
0.0ThrXaa: 0.0 ± 0.0
Val
7.611ValAla: 7.611 ± 0.769
0.876ValCys: 0.876 ± 0.22
3.395ValAsp: 3.395 ± 0.372
4.654ValGlu: 4.654 ± 0.624
2.135ValPhe: 2.135 ± 0.364
5.147ValGly: 5.147 ± 0.766
1.259ValHis: 1.259 ± 0.239
3.285ValIle: 3.285 ± 0.437
2.628ValLys: 2.628 ± 0.302
5.585ValLeu: 5.585 ± 0.7
1.807ValMet: 1.807 ± 0.273
2.026ValAsn: 2.026 ± 0.32
3.559ValPro: 3.559 ± 0.372
2.847ValGln: 2.847 ± 0.476
5.421ValArg: 5.421 ± 0.712
4.38ValSer: 4.38 ± 0.527
4.107ValThr: 4.107 ± 0.564
5.257ValVal: 5.257 ± 0.5
0.876ValTrp: 0.876 ± 0.248
1.697ValTyr: 1.697 ± 0.371
0.0ValXaa: 0.0 ± 0.0
Trp
2.026TrpAla: 2.026 ± 0.347
0.274TrpCys: 0.274 ± 0.119
1.04TrpAsp: 1.04 ± 0.255
0.657TrpGlu: 0.657 ± 0.208
0.712TrpPhe: 0.712 ± 0.212
0.821TrpGly: 0.821 ± 0.235
0.274TrpHis: 0.274 ± 0.118
0.438TrpIle: 0.438 ± 0.145
0.602TrpLys: 0.602 ± 0.173
1.314TrpLeu: 1.314 ± 0.265
0.329TrpMet: 0.329 ± 0.133
0.219TrpAsn: 0.219 ± 0.134
1.04TrpPro: 1.04 ± 0.254
0.602TrpGln: 0.602 ± 0.164
1.862TrpArg: 1.862 ± 0.323
1.259TrpSer: 1.259 ± 0.246
0.712TrpThr: 0.712 ± 0.2
1.588TrpVal: 1.588 ± 0.281
0.493TrpTrp: 0.493 ± 0.169
0.767TrpTyr: 0.767 ± 0.157
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.121TyrAla: 3.121 ± 0.388
0.438TyrCys: 0.438 ± 0.139
1.807TyrAsp: 1.807 ± 0.323
1.643TyrGlu: 1.643 ± 0.317
0.821TyrPhe: 0.821 ± 0.229
2.409TyrGly: 2.409 ± 0.333
0.602TyrHis: 0.602 ± 0.206
0.438TyrIle: 0.438 ± 0.153
1.15TyrLys: 1.15 ± 0.238
2.081TyrLeu: 2.081 ± 0.321
0.493TyrMet: 0.493 ± 0.181
0.876TyrAsn: 0.876 ± 0.247
0.986TyrPro: 0.986 ± 0.212
0.876TyrGln: 0.876 ± 0.213
2.081TyrArg: 2.081 ± 0.389
1.862TyrSer: 1.862 ± 0.243
2.3TyrThr: 2.3 ± 0.343
1.862TyrVal: 1.862 ± 0.284
0.493TyrTrp: 0.493 ± 0.187
0.602TyrTyr: 0.602 ± 0.14
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 97 proteins (18264 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski