Amino acid dipepetide frequency for Salmonella phage FSL SP-031

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
11.288AlaAla: 11.288 ± 1.425
1.033AlaCys: 1.033 ± 0.352
7.313AlaAsp: 7.313 ± 0.697
6.598AlaGlu: 6.598 ± 0.791
3.657AlaPhe: 3.657 ± 0.546
8.426AlaGly: 8.426 ± 0.74
1.59AlaHis: 1.59 ± 0.42
4.69AlaIle: 4.69 ± 0.641
5.803AlaLys: 5.803 ± 0.768
7.472AlaLeu: 7.472 ± 0.796
2.146AlaMet: 2.146 ± 0.509
4.134AlaAsn: 4.134 ± 0.663
3.259AlaPro: 3.259 ± 0.574
3.577AlaGln: 3.577 ± 0.703
5.087AlaArg: 5.087 ± 0.69
5.723AlaSer: 5.723 ± 0.741
6.518AlaThr: 6.518 ± 0.802
6.677AlaVal: 6.677 ± 0.828
1.272AlaTrp: 1.272 ± 0.244
3.259AlaTyr: 3.259 ± 0.629
0.0AlaXaa: 0.0 ± 0.0
Cys
0.954CysAla: 0.954 ± 0.321
0.238CysCys: 0.238 ± 0.137
0.715CysAsp: 0.715 ± 0.27
0.954CysGlu: 0.954 ± 0.287
0.0CysPhe: 0.0 ± 0.0
1.351CysGly: 1.351 ± 0.363
0.238CysHis: 0.238 ± 0.141
0.238CysIle: 0.238 ± 0.138
0.636CysLys: 0.636 ± 0.215
0.715CysLeu: 0.715 ± 0.276
0.238CysMet: 0.238 ± 0.147
0.636CysAsn: 0.636 ± 0.208
0.477CysPro: 0.477 ± 0.196
0.715CysGln: 0.715 ± 0.296
0.874CysArg: 0.874 ± 0.293
0.159CysSer: 0.159 ± 0.117
0.556CysThr: 0.556 ± 0.185
0.954CysVal: 0.954 ± 0.312
0.318CysTrp: 0.318 ± 0.153
0.715CysTyr: 0.715 ± 0.192
0.0CysXaa: 0.0 ± 0.0
Asp
6.916AspAla: 6.916 ± 0.673
0.477AspCys: 0.477 ± 0.172
4.372AspAsp: 4.372 ± 0.877
5.485AspGlu: 5.485 ± 1.007
2.385AspPhe: 2.385 ± 0.364
5.167AspGly: 5.167 ± 0.811
0.874AspHis: 0.874 ± 0.217
3.498AspIle: 3.498 ± 0.451
3.895AspLys: 3.895 ± 0.724
5.326AspLeu: 5.326 ± 0.881
1.51AspMet: 1.51 ± 0.397
2.544AspAsn: 2.544 ± 0.449
1.51AspPro: 1.51 ± 0.354
1.351AspGln: 1.351 ± 0.365
2.226AspArg: 2.226 ± 0.477
4.054AspSer: 4.054 ± 0.514
3.975AspThr: 3.975 ± 0.472
3.816AspVal: 3.816 ± 0.426
1.431AspTrp: 1.431 ± 0.353
1.987AspTyr: 1.987 ± 0.387
0.0AspXaa: 0.0 ± 0.0
Glu
6.439GluAla: 6.439 ± 0.899
0.477GluCys: 0.477 ± 0.181
4.134GluAsp: 4.134 ± 0.558
3.895GluGlu: 3.895 ± 0.667
2.226GluPhe: 2.226 ± 0.355
4.293GluGly: 4.293 ± 0.607
1.431GluHis: 1.431 ± 0.424
3.1GluIle: 3.1 ± 0.424
4.054GluLys: 4.054 ± 0.574
5.803GluLeu: 5.803 ± 0.794
2.941GluMet: 2.941 ± 0.4
2.862GluAsn: 2.862 ± 0.577
2.146GluPro: 2.146 ± 0.419
2.623GluGln: 2.623 ± 0.586
3.418GluArg: 3.418 ± 0.576
3.339GluSer: 3.339 ± 0.365
3.895GluThr: 3.895 ± 0.575
3.657GluVal: 3.657 ± 0.516
1.033GluTrp: 1.033 ± 0.254
2.067GluTyr: 2.067 ± 0.403
0.0GluXaa: 0.0 ± 0.0
Phe
3.1PheAla: 3.1 ± 0.424
0.477PheCys: 0.477 ± 0.194
2.782PheAsp: 2.782 ± 0.479
2.226PheGlu: 2.226 ± 0.351
0.636PhePhe: 0.636 ± 0.257
2.941PheGly: 2.941 ± 0.416
0.477PheHis: 0.477 ± 0.201
2.226PheIle: 2.226 ± 0.452
1.749PheLys: 1.749 ± 0.304
2.385PheLeu: 2.385 ± 0.494
0.318PheMet: 0.318 ± 0.134
1.987PheAsn: 1.987 ± 0.423
1.351PhePro: 1.351 ± 0.318
0.954PheGln: 0.954 ± 0.257
1.51PheArg: 1.51 ± 0.321
2.544PheSer: 2.544 ± 0.668
2.623PheThr: 2.623 ± 0.618
2.464PheVal: 2.464 ± 0.424
1.113PheTrp: 1.113 ± 0.324
1.272PheTyr: 1.272 ± 0.247
0.0PheXaa: 0.0 ± 0.0
Gly
7.154GlyAla: 7.154 ± 0.669
1.033GlyCys: 1.033 ± 0.313
4.293GlyAsp: 4.293 ± 0.712
4.213GlyGlu: 4.213 ± 0.537
3.339GlyPhe: 3.339 ± 0.433
6.041GlyGly: 6.041 ± 0.772
1.272GlyHis: 1.272 ± 0.312
3.657GlyIle: 3.657 ± 0.538
5.246GlyLys: 5.246 ± 0.704
6.359GlyLeu: 6.359 ± 0.659
1.987GlyMet: 1.987 ± 0.387
3.895GlyAsn: 3.895 ± 0.533
2.067GlyPro: 2.067 ± 0.345
2.941GlyGln: 2.941 ± 0.407
4.293GlyArg: 4.293 ± 0.599
5.167GlySer: 5.167 ± 0.665
4.69GlyThr: 4.69 ± 0.608
5.644GlyVal: 5.644 ± 0.592
1.113GlyTrp: 1.113 ± 0.319
2.782GlyTyr: 2.782 ± 0.434
0.0GlyXaa: 0.0 ± 0.0
His
1.272HisAla: 1.272 ± 0.273
0.318HisCys: 0.318 ± 0.161
0.715HisAsp: 0.715 ± 0.225
0.715HisGlu: 0.715 ± 0.236
1.033HisPhe: 1.033 ± 0.322
0.715HisGly: 0.715 ± 0.272
0.556HisHis: 0.556 ± 0.166
1.192HisIle: 1.192 ± 0.372
0.795HisLys: 0.795 ± 0.257
1.272HisLeu: 1.272 ± 0.296
0.318HisMet: 0.318 ± 0.241
1.192HisAsn: 1.192 ± 0.293
1.351HisPro: 1.351 ± 0.386
0.954HisGln: 0.954 ± 0.267
0.795HisArg: 0.795 ± 0.235
1.113HisSer: 1.113 ± 0.246
0.636HisThr: 0.636 ± 0.234
1.192HisVal: 1.192 ± 0.305
0.238HisTrp: 0.238 ± 0.134
0.477HisTyr: 0.477 ± 0.2
0.0HisXaa: 0.0 ± 0.0
Ile
5.326IleAla: 5.326 ± 0.842
1.113IleCys: 1.113 ± 0.298
3.657IleAsp: 3.657 ± 0.557
3.736IleGlu: 3.736 ± 0.428
1.351IlePhe: 1.351 ± 0.475
3.657IleGly: 3.657 ± 0.521
0.318IleHis: 0.318 ± 0.183
3.021IleIle: 3.021 ± 0.372
3.577IleLys: 3.577 ± 0.506
3.021IleLeu: 3.021 ± 0.51
1.351IleMet: 1.351 ± 0.261
2.703IleAsn: 2.703 ± 0.561
2.703IlePro: 2.703 ± 0.417
1.828IleGln: 1.828 ± 0.445
2.385IleArg: 2.385 ± 0.406
3.259IleSer: 3.259 ± 0.623
3.577IleThr: 3.577 ± 0.471
2.782IleVal: 2.782 ± 0.461
0.636IleTrp: 0.636 ± 0.248
1.51IleTyr: 1.51 ± 0.347
0.0IleXaa: 0.0 ± 0.0
Lys
5.405LysAla: 5.405 ± 0.812
0.954LysCys: 0.954 ± 0.293
2.464LysAsp: 2.464 ± 0.43
3.816LysGlu: 3.816 ± 0.514
1.669LysPhe: 1.669 ± 0.314
4.213LysGly: 4.213 ± 0.636
2.146LysHis: 2.146 ± 0.448
2.703LysIle: 2.703 ± 0.459
2.782LysLys: 2.782 ± 0.407
4.531LysLeu: 4.531 ± 0.691
2.067LysMet: 2.067 ± 0.489
2.623LysAsn: 2.623 ± 0.536
3.259LysPro: 3.259 ± 0.492
2.623LysGln: 2.623 ± 0.394
3.498LysArg: 3.498 ± 0.527
3.339LysSer: 3.339 ± 0.579
4.054LysThr: 4.054 ± 0.536
3.18LysVal: 3.18 ± 0.462
1.113LysTrp: 1.113 ± 0.258
2.782LysTyr: 2.782 ± 0.413
0.0LysXaa: 0.0 ± 0.0
Leu
8.108LeuAla: 8.108 ± 0.786
0.715LeuCys: 0.715 ± 0.264
4.372LeuAsp: 4.372 ± 0.465
4.372LeuGlu: 4.372 ± 0.551
2.305LeuPhe: 2.305 ± 0.365
4.372LeuGly: 4.372 ± 0.529
1.033LeuHis: 1.033 ± 0.336
2.862LeuIle: 2.862 ± 0.462
5.564LeuLys: 5.564 ± 0.812
6.041LeuLeu: 6.041 ± 0.813
1.908LeuMet: 1.908 ± 0.366
5.087LeuAsn: 5.087 ± 0.56
3.339LeuPro: 3.339 ± 0.6
3.18LeuGln: 3.18 ± 0.484
5.087LeuArg: 5.087 ± 0.624
4.372LeuSer: 4.372 ± 0.585
4.372LeuThr: 4.372 ± 0.707
5.405LeuVal: 5.405 ± 0.779
0.715LeuTrp: 0.715 ± 0.232
2.305LeuTyr: 2.305 ± 0.379
0.0LeuXaa: 0.0 ± 0.0
Met
2.782MetAla: 2.782 ± 0.58
0.318MetCys: 0.318 ± 0.151
1.351MetAsp: 1.351 ± 0.466
1.59MetGlu: 1.59 ± 0.369
1.113MetPhe: 1.113 ± 0.252
1.59MetGly: 1.59 ± 0.314
0.556MetHis: 0.556 ± 0.2
1.033MetIle: 1.033 ± 0.272
1.351MetLys: 1.351 ± 0.276
1.828MetLeu: 1.828 ± 0.365
1.113MetMet: 1.113 ± 0.343
1.113MetAsn: 1.113 ± 0.362
1.113MetPro: 1.113 ± 0.301
1.113MetGln: 1.113 ± 0.251
1.908MetArg: 1.908 ± 0.539
1.351MetSer: 1.351 ± 0.307
2.305MetThr: 2.305 ± 0.439
1.351MetVal: 1.351 ± 0.342
0.318MetTrp: 0.318 ± 0.148
0.715MetTyr: 0.715 ± 0.245
0.0MetXaa: 0.0 ± 0.0
Asn
4.213AsnAla: 4.213 ± 0.575
0.397AsnCys: 0.397 ± 0.162
3.736AsnAsp: 3.736 ± 0.408
2.464AsnGlu: 2.464 ± 0.405
1.431AsnPhe: 1.431 ± 0.325
5.485AsnGly: 5.485 ± 0.638
0.715AsnHis: 0.715 ± 0.23
3.418AsnIle: 3.418 ± 0.47
2.623AsnLys: 2.623 ± 0.524
3.021AsnLeu: 3.021 ± 0.355
0.795AsnMet: 0.795 ± 0.217
2.862AsnAsn: 2.862 ± 0.559
1.669AsnPro: 1.669 ± 0.428
1.59AsnGln: 1.59 ± 0.4
2.226AsnArg: 2.226 ± 0.443
2.782AsnSer: 2.782 ± 0.513
3.259AsnThr: 3.259 ± 0.469
3.339AsnVal: 3.339 ± 0.522
0.715AsnTrp: 0.715 ± 0.275
1.59AsnTyr: 1.59 ± 0.321
0.0AsnXaa: 0.0 ± 0.0
Pro
3.021ProAla: 3.021 ± 0.533
0.477ProCys: 0.477 ± 0.223
2.941ProAsp: 2.941 ± 0.423
4.372ProGlu: 4.372 ± 0.488
1.828ProPhe: 1.828 ± 0.372
2.067ProGly: 2.067 ± 0.716
0.477ProHis: 0.477 ± 0.205
1.828ProIle: 1.828 ± 0.44
1.987ProLys: 1.987 ± 0.391
3.1ProLeu: 3.1 ± 0.507
1.113ProMet: 1.113 ± 0.293
1.669ProAsn: 1.669 ± 0.557
0.795ProPro: 0.795 ± 0.241
1.351ProGln: 1.351 ± 0.395
1.669ProArg: 1.669 ± 0.401
1.987ProSer: 1.987 ± 0.334
2.146ProThr: 2.146 ± 0.334
3.657ProVal: 3.657 ± 0.438
0.159ProTrp: 0.159 ± 0.116
1.351ProTyr: 1.351 ± 0.369
0.0ProXaa: 0.0 ± 0.0
Gln
4.61GlnAla: 4.61 ± 0.513
0.477GlnCys: 0.477 ± 0.174
2.146GlnAsp: 2.146 ± 0.474
2.385GlnGlu: 2.385 ± 0.471
1.351GlnPhe: 1.351 ± 0.32
1.828GlnGly: 1.828 ± 0.411
0.954GlnHis: 0.954 ± 0.302
2.544GlnIle: 2.544 ± 0.439
1.987GlnLys: 1.987 ± 0.472
2.623GlnLeu: 2.623 ± 0.466
1.351GlnMet: 1.351 ± 0.309
1.669GlnAsn: 1.669 ± 0.372
1.987GlnPro: 1.987 ± 0.487
2.544GlnGln: 2.544 ± 0.674
2.941GlnArg: 2.941 ± 0.499
1.669GlnSer: 1.669 ± 0.359
2.385GlnThr: 2.385 ± 0.425
2.782GlnVal: 2.782 ± 0.442
0.556GlnTrp: 0.556 ± 0.245
1.59GlnTyr: 1.59 ± 0.273
0.0GlnXaa: 0.0 ± 0.0
Arg
4.61ArgAla: 4.61 ± 0.57
0.397ArgCys: 0.397 ± 0.17
3.1ArgAsp: 3.1 ± 0.583
2.623ArgGlu: 2.623 ± 0.469
1.987ArgPhe: 1.987 ± 0.322
4.849ArgGly: 4.849 ± 0.666
0.795ArgHis: 0.795 ± 0.199
2.862ArgIle: 2.862 ± 0.375
3.895ArgLys: 3.895 ± 0.521
3.816ArgLeu: 3.816 ± 0.469
1.192ArgMet: 1.192 ± 0.366
3.498ArgAsn: 3.498 ± 0.513
2.067ArgPro: 2.067 ± 0.459
3.1ArgGln: 3.1 ± 0.455
3.577ArgArg: 3.577 ± 0.631
2.464ArgSer: 2.464 ± 0.442
2.941ArgThr: 2.941 ± 0.417
4.054ArgVal: 4.054 ± 0.525
0.636ArgTrp: 0.636 ± 0.271
1.113ArgTyr: 1.113 ± 0.368
0.0ArgXaa: 0.0 ± 0.0
Ser
5.405SerAla: 5.405 ± 0.866
0.397SerCys: 0.397 ± 0.204
4.213SerAsp: 4.213 ± 0.642
2.623SerGlu: 2.623 ± 0.552
2.623SerPhe: 2.623 ± 0.392
4.769SerGly: 4.769 ± 0.641
1.351SerHis: 1.351 ± 0.378
3.418SerIle: 3.418 ± 0.447
2.703SerLys: 2.703 ± 0.418
4.134SerLeu: 4.134 ± 0.503
1.351SerMet: 1.351 ± 0.266
2.305SerAsn: 2.305 ± 0.355
2.305SerPro: 2.305 ± 0.535
2.146SerGln: 2.146 ± 0.567
3.259SerArg: 3.259 ± 0.395
3.498SerSer: 3.498 ± 0.61
4.213SerThr: 4.213 ± 0.592
3.895SerVal: 3.895 ± 0.82
0.874SerTrp: 0.874 ± 0.196
1.908SerTyr: 1.908 ± 0.335
0.0SerXaa: 0.0 ± 0.0
Thr
7.631ThrAla: 7.631 ± 0.819
0.715ThrCys: 0.715 ± 0.241
2.862ThrAsp: 2.862 ± 0.418
4.054ThrGlu: 4.054 ± 0.592
2.623ThrPhe: 2.623 ± 0.521
7.234ThrGly: 7.234 ± 0.944
0.477ThrHis: 0.477 ± 0.181
3.577ThrIle: 3.577 ± 0.425
3.1ThrLys: 3.1 ± 0.493
5.008ThrLeu: 5.008 ± 0.626
1.351ThrMet: 1.351 ± 0.319
2.146ThrAsn: 2.146 ± 0.504
3.18ThrPro: 3.18 ± 0.598
2.226ThrGln: 2.226 ± 0.386
2.464ThrArg: 2.464 ± 0.33
3.975ThrSer: 3.975 ± 0.69
4.69ThrThr: 4.69 ± 0.807
5.405ThrVal: 5.405 ± 0.809
1.113ThrTrp: 1.113 ± 0.314
1.987ThrTyr: 1.987 ± 0.444
0.0ThrXaa: 0.0 ± 0.0
Val
6.836ValAla: 6.836 ± 0.748
0.795ValCys: 0.795 ± 0.262
4.213ValAsp: 4.213 ± 0.441
4.69ValGlu: 4.69 ± 0.66
1.59ValPhe: 1.59 ± 0.405
4.054ValGly: 4.054 ± 0.579
0.636ValHis: 0.636 ± 0.212
4.054ValIle: 4.054 ± 0.458
4.452ValLys: 4.452 ± 0.592
4.769ValLeu: 4.769 ± 0.58
1.749ValMet: 1.749 ± 0.411
3.18ValAsn: 3.18 ± 0.535
1.908ValPro: 1.908 ± 0.398
2.941ValGln: 2.941 ± 0.482
3.975ValArg: 3.975 ± 0.549
3.975ValSer: 3.975 ± 0.628
5.803ValThr: 5.803 ± 0.847
5.246ValVal: 5.246 ± 0.838
1.033ValTrp: 1.033 ± 0.323
2.544ValTyr: 2.544 ± 0.387
0.0ValXaa: 0.0 ± 0.0
Trp
0.954TrpAla: 0.954 ± 0.461
0.238TrpCys: 0.238 ± 0.138
0.795TrpAsp: 0.795 ± 0.217
0.556TrpGlu: 0.556 ± 0.197
0.715TrpPhe: 0.715 ± 0.202
1.113TrpGly: 1.113 ± 0.4
0.477TrpHis: 0.477 ± 0.203
0.556TrpIle: 0.556 ± 0.198
0.715TrpLys: 0.715 ± 0.235
1.908TrpLeu: 1.908 ± 0.442
0.238TrpMet: 0.238 ± 0.137
0.795TrpAsn: 0.795 ± 0.235
0.397TrpPro: 0.397 ± 0.21
0.715TrpGln: 0.715 ± 0.268
1.033TrpArg: 1.033 ± 0.283
1.192TrpSer: 1.192 ± 0.292
0.874TrpThr: 0.874 ± 0.248
1.113TrpVal: 1.113 ± 0.278
0.238TrpTrp: 0.238 ± 0.13
0.556TrpTyr: 0.556 ± 0.211
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.498TyrAla: 3.498 ± 0.594
0.636TyrCys: 0.636 ± 0.206
2.623TyrAsp: 2.623 ± 0.47
2.464TyrGlu: 2.464 ± 0.484
1.272TyrPhe: 1.272 ± 0.313
3.021TyrGly: 3.021 ± 0.475
0.477TyrHis: 0.477 ± 0.175
1.033TyrIle: 1.033 ± 0.237
2.305TyrLys: 2.305 ± 0.355
2.385TyrLeu: 2.385 ± 0.376
0.795TyrMet: 0.795 ± 0.216
1.431TyrAsn: 1.431 ± 0.302
1.113TyrPro: 1.113 ± 0.344
1.908TyrGln: 1.908 ± 0.392
1.351TyrArg: 1.351 ± 0.307
1.51TyrSer: 1.51 ± 0.365
2.305TyrThr: 2.305 ± 0.461
1.828TyrVal: 1.828 ± 0.397
0.556TyrTrp: 0.556 ± 0.179
1.192TyrTyr: 1.192 ± 0.29
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 59 proteins (12581 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski