Tuber magnatum (white Piedmont truffle)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; Pezizomycetes; Pezizales; Tuberaceae; Tuber

Average proteome isoelectric point is 6.83

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9390 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A317SDW5|A0A317SDW5_9PEZI Uncharacterized protein OS=Tuber magnatum OX=42249 GN=C7212DRAFT_348210 PE=4 SV=1
MM1 pKa = 8.08DD2 pKa = 5.95LPTTNSISKK11 pKa = 10.25SSSPDD16 pKa = 2.74TDD18 pKa = 5.98ANTTWLAQDD27 pKa = 3.78ISTLPDD33 pKa = 3.35SLEE36 pKa = 4.11LPEE39 pKa = 5.44LCDD42 pKa = 4.52FGIDD46 pKa = 3.73TVLEE50 pKa = 4.23LCDD53 pKa = 5.86FEE55 pKa = 6.51LCDD58 pKa = 4.73FDD60 pKa = 6.33IDD62 pKa = 4.94ILPEE66 pKa = 4.33LCDD69 pKa = 4.27FDD71 pKa = 6.1IDD73 pKa = 4.28TLPEE77 pKa = 4.3LCDD80 pKa = 4.13FDD82 pKa = 6.08IDD84 pKa = 4.22TLPEE88 pKa = 4.06SCDD91 pKa = 3.46ANTNTLSEE99 pKa = 4.41YY100 pKa = 11.2CNIDD104 pKa = 4.01PNSLPPLQDD113 pKa = 3.38FLEE116 pKa = 4.48LLSEE120 pKa = 4.23PMDD123 pKa = 3.83VEE125 pKa = 4.49SLCASWNLGMGSDD138 pKa = 3.69EE139 pKa = 4.75TICKK143 pKa = 10.31VGEE146 pKa = 4.47DD147 pKa = 3.98DD148 pKa = 5.43DD149 pKa = 6.56AGILEE154 pKa = 4.21WTGDD158 pKa = 3.65EE159 pKa = 4.21EE160 pKa = 5.32HH161 pKa = 6.97FGRR164 pKa = 11.84PNCTSEE170 pKa = 4.34GSVSGSHH177 pKa = 7.04RR178 pKa = 11.84LTNQEE183 pKa = 3.95LAEE186 pKa = 3.94QLKK189 pKa = 9.25QLKK192 pKa = 10.31GDD194 pKa = 4.19LEE196 pKa = 4.33QVTRR200 pKa = 11.84ALVVLDD206 pKa = 4.01SYY208 pKa = 10.31IAQMIPWSFSIHH220 pKa = 5.59NAVHH224 pKa = 5.81KK225 pKa = 9.78TNTLPAGVLGSPNPTPRR242 pKa = 4.4

Molecular weight: 26.58 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A317SVA2|A0A317SVA2_9PEZI Probable vacuolar protein sorting-associated protein 16 homolog OS=Tuber magnatum OX=42249 GN=C7212DRAFT_296902 PE=3 SV=1
MM1 pKa = 7.94PSQKK5 pKa = 10.41SFRR8 pKa = 11.84TKK10 pKa = 10.38QKK12 pKa = 9.84LAKK15 pKa = 9.55AQKK18 pKa = 8.59QNRR21 pKa = 11.84PIPQWIRR28 pKa = 11.84LRR30 pKa = 11.84TGNTIRR36 pKa = 11.84YY37 pKa = 5.79NAKK40 pKa = 8.89RR41 pKa = 11.84RR42 pKa = 11.84HH43 pKa = 4.15WRR45 pKa = 11.84KK46 pKa = 7.38TRR48 pKa = 11.84IGII51 pKa = 4.0

Molecular weight: 6.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9390

0

9390

3818676

49

4889

406.7

44.97

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.951 ± 0.021

1.269 ± 0.01

5.294 ± 0.017

6.715 ± 0.026

3.598 ± 0.015

7.604 ± 0.023

2.32 ± 0.014

4.975 ± 0.015

5.218 ± 0.023

8.885 ± 0.03

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.133 ± 0.009

3.612 ± 0.015

6.198 ± 0.03

3.527 ± 0.022

6.435 ± 0.022

8.092 ± 0.027

5.838 ± 0.018

6.269 ± 0.02

1.357 ± 0.008

2.708 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski