Amino acid dipepetide frequency for Azoarcus sp. M9-3-2

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	16.93AlaAla: 16.93 ± 0.165	1.44AlaCys: 1.44 ± 0.033	7.579AlaAsp: 7.579 ± 0.103	7.124AlaGlu: 7.124 ± 0.083	4.21AlaPhe: 4.21 ± 0.054	10.873AlaGly: 10.873 ± 0.129	3.164AlaHis: 3.164 ± 0.053	5.675AlaIle: 5.675 ± 0.082	3.087AlaLys: 3.087 ± 0.058	14.807AlaLeu: 14.807 ± 0.152	3.384AlaMet: 3.384 ± 0.057	2.77AlaAsn: 2.77 ± 0.064	6.443AlaPro: 6.443 ± 0.097	4.713AlaGln: 4.713 ± 0.069	10.351AlaArg: 10.351 ± 0.136	5.93AlaSer: 5.93 ± 0.089	6.069AlaThr: 6.069 ± 0.113	8.879AlaVal: 8.879 ± 0.086	1.842AlaTrp: 1.842 ± 0.044	2.54AlaTyr: 2.54 ± 0.047	0.0AlaXaa: 0.0 ± 0.0
Cys	1.29CysAla: 1.29 ± 0.036	0.143CysCys: 0.143 ± 0.014	0.631CysAsp: 0.631 ± 0.028	0.579CysGlu: 0.579 ± 0.027	0.371CysPhe: 0.371 ± 0.018	0.987CysGly: 0.987 ± 0.031	0.399CysHis: 0.399 ± 0.043	0.401CysIle: 0.401 ± 0.019	0.212CysLys: 0.212 ± 0.014	1.018CysLeu: 1.018 ± 0.03	0.192CysMet: 0.192 ± 0.012	0.241CysAsn: 0.241 ± 0.013	0.578CysPro: 0.578 ± 0.022	0.268CysGln: 0.268 ± 0.014	0.733CysArg: 0.733 ± 0.026	0.506CysSer: 0.506 ± 0.024	0.472CysThr: 0.472 ± 0.02	0.745CysVal: 0.745 ± 0.026	0.122CysTrp: 0.122 ± 0.01	0.229CysTyr: 0.229 ± 0.014	0.0CysXaa: 0.0 ± 0.0
Asp	8.32AspAla: 8.32 ± 0.109	0.54AspCys: 0.54 ± 0.02	3.521AspAsp: 3.521 ± 0.073	3.696AspGlu: 3.696 ± 0.057	2.196AspPhe: 2.196 ± 0.043	5.412AspGly: 5.412 ± 0.127	1.349AspHis: 1.349 ± 0.033	2.739AspIle: 2.739 ± 0.054	1.726AspLys: 1.726 ± 0.04	5.744AspLeu: 5.744 ± 0.065	1.43AspMet: 1.43 ± 0.034	1.293AspAsn: 1.293 ± 0.038	3.45AspPro: 3.45 ± 0.058	1.681AspGln: 1.681 ± 0.036	4.053AspArg: 4.053 ± 0.056	2.245AspSer: 2.245 ± 0.054	3.435AspThr: 3.435 ± 0.068	4.222AspVal: 4.222 ± 0.063	1.046AspTrp: 1.046 ± 0.031	1.539AspTyr: 1.539 ± 0.037	0.0AspXaa: 0.0 ± 0.0
Glu	8.35GluAla: 8.35 ± 0.104	0.452GluCys: 0.452 ± 0.022	2.72GluAsp: 2.72 ± 0.05	2.57GluGlu: 2.57 ± 0.064	1.753GluPhe: 1.753 ± 0.041	3.998GluGly: 3.998 ± 0.062	1.572GluHis: 1.572 ± 0.036	2.777GluIle: 2.777 ± 0.051	1.647GluLys: 1.647 ± 0.049	5.675GluLeu: 5.675 ± 0.079	1.311GluMet: 1.311 ± 0.039	1.316GluAsn: 1.316 ± 0.032	2.485GluPro: 2.485 ± 0.048	2.396GluGln: 2.396 ± 0.047	5.193GluArg: 5.193 ± 0.075	2.564GluSer: 2.564 ± 0.046	2.958GluThr: 2.958 ± 0.054	4.309GluVal: 4.309 ± 0.073	0.725GluTrp: 0.725 ± 0.024	1.109GluTyr: 1.109 ± 0.034	0.0GluXaa: 0.0 ± 0.0
Phe	4.335PheAla: 4.335 ± 0.056	0.405PheCys: 0.405 ± 0.017	2.757PheAsp: 2.757 ± 0.04	2.236PheGlu: 2.236 ± 0.038	1.419PhePhe: 1.419 ± 0.039	3.44PheGly: 3.44 ± 0.057	0.788PheHis: 0.788 ± 0.026	1.568PheIle: 1.568 ± 0.038	1.028PheLys: 1.028 ± 0.032	3.134PheLeu: 3.134 ± 0.056	0.838PheMet: 0.838 ± 0.028	1.06PheAsn: 1.06 ± 0.032	1.44PhePro: 1.44 ± 0.033	0.915PheGln: 0.915 ± 0.033	2.108PheArg: 2.108 ± 0.043	2.121PheSer: 2.121 ± 0.046	1.794PheThr: 1.794 ± 0.038	2.915PheVal: 2.915 ± 0.054	0.465PheTrp: 0.465 ± 0.021	0.914PheTyr: 0.914 ± 0.028	0.0PheXaa: 0.0 ± 0.0
Gly	9.581GlyAla: 9.581 ± 0.152	0.985GlyCys: 0.985 ± 0.029	4.609GlyAsp: 4.609 ± 0.076	4.939GlyGlu: 4.939 ± 0.068	3.44GlyPhe: 3.44 ± 0.062	6.928GlyGly: 6.928 ± 0.14	2.198GlyHis: 2.198 ± 0.047	4.115GlyIle: 4.115 ± 0.065	2.988GlyLys: 2.988 ± 0.059	8.626GlyLeu: 8.626 ± 0.096	2.278GlyMet: 2.278 ± 0.049	2.117GlyAsn: 2.117 ± 0.07	2.965GlyPro: 2.965 ± 0.052	3.086GlyGln: 3.086 ± 0.05	6.146GlyArg: 6.146 ± 0.076	4.029GlySer: 4.029 ± 0.097	4.434GlyThr: 4.434 ± 0.088	6.628GlyVal: 6.628 ± 0.075	1.34GlyTrp: 1.34 ± 0.037	2.21GlyTyr: 2.21 ± 0.045	0.0GlyXaa: 0.0 ± 0.0
His	2.972HisAla: 2.972 ± 0.052	0.267HisCys: 0.267 ± 0.015	1.447HisAsp: 1.447 ± 0.035	1.29HisGlu: 1.29 ± 0.035	1.04HisPhe: 1.04 ± 0.028	2.358HisGly: 2.358 ± 0.046	0.736HisHis: 0.736 ± 0.025	0.995HisIle: 0.995 ± 0.03	0.571HisLys: 0.571 ± 0.024	2.713HisLeu: 2.713 ± 0.046	0.559HisMet: 0.559 ± 0.02	0.526HisAsn: 0.526 ± 0.023	1.722HisPro: 1.722 ± 0.04	0.745HisGln: 0.745 ± 0.029	1.809HisArg: 1.809 ± 0.04	0.954HisSer: 0.954 ± 0.027	1.239HisThr: 1.239 ± 0.034	1.728HisVal: 1.728 ± 0.038	0.455HisTrp: 0.455 ± 0.021	0.659HisTyr: 0.659 ± 0.023	0.0HisXaa: 0.0 ± 0.0
Ile	6.033IleAla: 6.033 ± 0.071	0.461IleCys: 0.461 ± 0.019	3.716IleAsp: 3.716 ± 0.055	3.414IleGlu: 3.414 ± 0.057	1.387IlePhe: 1.387 ± 0.037	4.443IleGly: 4.443 ± 0.061	1.063IleHis: 1.063 ± 0.028	1.729IleIle: 1.729 ± 0.043	1.377IleLys: 1.377 ± 0.034	3.701IleLeu: 3.701 ± 0.069	0.8IleMet: 0.8 ± 0.031	1.432IleAsn: 1.432 ± 0.035	2.194IlePro: 2.194 ± 0.046	1.244IleGln: 1.244 ± 0.034	3.195IleArg: 3.195 ± 0.05	2.313IleSer: 2.313 ± 0.053	2.475IleThr: 2.475 ± 0.072	3.585IleVal: 3.585 ± 0.059	0.426IleTrp: 0.426 ± 0.019	1.014IleTyr: 1.014 ± 0.033	0.0IleXaa: 0.0 ± 0.0
Lys	3.479LysAla: 3.479 ± 0.063	0.186LysCys: 0.186 ± 0.014	1.496LysAsp: 1.496 ± 0.033	1.36LysGlu: 1.36 ± 0.045	0.774LysPhe: 0.774 ± 0.028	2.223LysGly: 2.223 ± 0.046	0.702LysHis: 0.702 ± 0.026	1.397LysIle: 1.397 ± 0.041	1.055LysLys: 1.055 ± 0.038	2.891LysLeu: 2.891 ± 0.064	0.698LysMet: 0.698 ± 0.025	0.744LysAsn: 0.744 ± 0.025	1.68LysPro: 1.68 ± 0.041	1.11LysGln: 1.11 ± 0.032	2.164LysArg: 2.164 ± 0.047	1.469LysSer: 1.469 ± 0.039	1.605LysThr: 1.605 ± 0.041	2.204LysVal: 2.204 ± 0.046	0.337LysTrp: 0.337 ± 0.016	0.655LysTyr: 0.655 ± 0.025	0.0LysXaa: 0.0 ± 0.0
Leu	14.619LeuAla: 14.619 ± 0.148	1.032LeuCys: 1.032 ± 0.031	6.728LeuAsp: 6.728 ± 0.086	5.785LeuGlu: 5.785 ± 0.079	3.676LeuPhe: 3.676 ± 0.067	8.661LeuGly: 8.661 ± 0.111	2.237LeuHis: 2.237 ± 0.043	4.816LeuIle: 4.816 ± 0.082	3.248LeuLys: 3.248 ± 0.054	10.223LeuLeu: 10.223 ± 0.152	2.548LeuMet: 2.548 ± 0.048	2.62LeuAsn: 2.62 ± 0.063	5.738LeuPro: 5.738 ± 0.073	3.115LeuGln: 3.115 ± 0.047	7.681LeuArg: 7.681 ± 0.102	5.838LeuSer: 5.838 ± 0.079	5.578LeuThr: 5.578 ± 0.076	7.906LeuVal: 7.906 ± 0.115	1.301LeuTrp: 1.301 ± 0.042	2.082LeuTyr: 2.082 ± 0.042	0.0LeuXaa: 0.0 ± 0.0
Met	3.145MetAla: 3.145 ± 0.055	0.194MetCys: 0.194 ± 0.012	1.302MetAsp: 1.302 ± 0.03	1.086MetGlu: 1.086 ± 0.032	0.682MetPhe: 0.682 ± 0.024	1.76MetGly: 1.76 ± 0.041	0.59MetHis: 0.59 ± 0.021	1.109MetIle: 1.109 ± 0.032	0.925MetLys: 0.925 ± 0.03	2.562MetLeu: 2.562 ± 0.053	0.597MetMet: 0.597 ± 0.024	0.808MetAsn: 0.808 ± 0.028	1.467MetPro: 1.467 ± 0.036	0.898MetGln: 0.898 ± 0.03	1.814MetArg: 1.814 ± 0.037	1.605MetSer: 1.605 ± 0.034	1.52MetThr: 1.52 ± 0.033	1.703MetVal: 1.703 ± 0.038	0.204MetTrp: 0.204 ± 0.013	0.357MetTyr: 0.357 ± 0.02	0.0MetXaa: 0.0 ± 0.0
Asn	3.179AsnAla: 3.179 ± 0.074	0.283AsnCys: 0.283 ± 0.017	1.391AsnAsp: 1.391 ± 0.045	1.127AsnGlu: 1.127 ± 0.032	0.863AsnPhe: 0.863 ± 0.026	2.145AsnGly: 2.145 ± 0.06	0.564AsnHis: 0.564 ± 0.025	1.133AsnIle: 1.133 ± 0.031	0.678AsnLys: 0.678 ± 0.028	2.483AsnLeu: 2.483 ± 0.052	0.506AsnMet: 0.506 ± 0.019	0.635AsnAsn: 0.635 ± 0.023	1.718AsnPro: 1.718 ± 0.042	0.776AsnGln: 0.776 ± 0.026	1.775AsnArg: 1.775 ± 0.037	1.007AsnSer: 1.007 ± 0.042	1.397AsnThr: 1.397 ± 0.053	1.792AsnVal: 1.792 ± 0.042	0.344AsnTrp: 0.344 ± 0.018	0.597AsnTyr: 0.597 ± 0.023	0.0AsnXaa: 0.0 ± 0.0
Pro	6.947ProAla: 6.947 ± 0.087	0.435ProCys: 0.435 ± 0.02	3.715ProAsp: 3.715 ± 0.053	3.604ProGlu: 3.604 ± 0.059	1.715ProPhe: 1.715 ± 0.035	4.591ProGly: 4.591 ± 0.068	1.148ProHis: 1.148 ± 0.03	2.106ProIle: 2.106 ± 0.049	1.334ProLys: 1.334 ± 0.037	5.061ProLeu: 5.061 ± 0.069	1.329ProMet: 1.329 ± 0.032	1.206ProAsn: 1.206 ± 0.038	2.833ProPro: 2.833 ± 0.072	1.517ProGln: 1.517 ± 0.038	3.056ProArg: 3.056 ± 0.056	2.339ProSer: 2.339 ± 0.045	2.543ProThr: 2.543 ± 0.048	4.593ProVal: 4.593 ± 0.06	0.763ProTrp: 0.763 ± 0.029	1.105ProTyr: 1.105 ± 0.027	0.0ProXaa: 0.0 ± 0.0
Gln	4.609GlnAla: 4.609 ± 0.069	0.28GlnCys: 0.28 ± 0.016	1.443GlnAsp: 1.443 ± 0.033	1.359GlnGlu: 1.359 ± 0.038	1.116GlnPhe: 1.116 ± 0.031	2.465GlnGly: 2.465 ± 0.047	0.813GlnHis: 0.813 ± 0.027	1.696GlnIle: 1.696 ± 0.041	0.848GlnLys: 0.848 ± 0.029	3.71GlnLeu: 3.71 ± 0.051	0.999GlnMet: 0.999 ± 0.029	0.686GlnAsn: 0.686 ± 0.024	1.76GlnPro: 1.76 ± 0.04	1.347GlnGln: 1.347 ± 0.038	2.83GlnArg: 2.83 ± 0.05	1.557GlnSer: 1.557 ± 0.035	1.749GlnThr: 1.749 ± 0.035	2.747GlnVal: 2.747 ± 0.048	0.574GlnTrp: 0.574 ± 0.02	0.698GlnTyr: 0.698 ± 0.018	0.0GlnXaa: 0.0 ± 0.0
Arg	8.475ArgAla: 8.475 ± 0.105	0.746ArgCys: 0.746 ± 0.027	4.235ArgAsp: 4.235 ± 0.059	4.38ArgGlu: 4.38 ± 0.079	3.308ArgPhe: 3.308 ± 0.054	4.779ArgGly: 4.779 ± 0.065	2.316ArgHis: 2.316 ± 0.057	4.195ArgIle: 4.195 ± 0.063	2.066ArgLys: 2.066 ± 0.047	8.95ArgLeu: 8.95 ± 0.106	1.979ArgMet: 1.979 ± 0.042	1.787ArgAsn: 1.787 ± 0.042	3.515ArgPro: 3.515 ± 0.059	2.915ArgGln: 2.915 ± 0.054	6.085ArgArg: 6.085 ± 0.104	3.222ArgSer: 3.222 ± 0.064	3.503ArgThr: 3.503 ± 0.054	5.494ArgVal: 5.494 ± 0.074	1.195ArgTrp: 1.195 ± 0.035	2.038ArgTyr: 2.038 ± 0.046	0.0ArgXaa: 0.0 ± 0.0
Ser	6.086SerAla: 6.086 ± 0.093	0.486SerCys: 0.486 ± 0.038	2.612SerAsp: 2.612 ± 0.049	2.513SerGlu: 2.513 ± 0.05	1.771SerPhe: 1.771 ± 0.042	4.929SerGly: 4.929 ± 0.094	1.151SerHis: 1.151 ± 0.032	2.244SerIle: 2.244 ± 0.045	1.22SerLys: 1.22 ± 0.036	5.073SerLeu: 5.073 ± 0.072	1.222SerMet: 1.222 ± 0.033	1.231SerAsn: 1.231 ± 0.035	2.612SerPro: 2.612 ± 0.044	1.364SerGln: 1.364 ± 0.034	3.516SerArg: 3.516 ± 0.054	2.446SerSer: 2.446 ± 0.057	2.617SerThr: 2.617 ± 0.072	3.773SerVal: 3.773 ± 0.064	0.618SerTrp: 0.618 ± 0.023	1.066SerTyr: 1.066 ± 0.045	0.0SerXaa: 0.0 ± 0.0
Thr	5.91ThrAla: 5.91 ± 0.092	0.511ThrCys: 0.511 ± 0.032	3.035ThrAsp: 3.035 ± 0.098	2.708ThrGlu: 2.708 ± 0.047	1.598ThrPhe: 1.598 ± 0.038	4.963ThrGly: 4.963 ± 0.088	1.305ThrHis: 1.305 ± 0.034	2.163ThrIle: 2.163 ± 0.059	1.057ThrLys: 1.057 ± 0.03	6.557ThrLeu: 6.557 ± 0.111	0.973ThrMet: 0.973 ± 0.024	1.14ThrAsn: 1.14 ± 0.035	3.577ThrPro: 3.577 ± 0.051	1.572ThrGln: 1.572 ± 0.039	3.913ThrArg: 3.913 ± 0.06	2.249ThrSer: 2.249 ± 0.058	2.69ThrThr: 2.69 ± 0.071	4.386ThrVal: 4.386 ± 0.085	0.673ThrTrp: 0.673 ± 0.026	1.135ThrTyr: 1.135 ± 0.043	0.0ThrXaa: 0.0 ± 0.0
Val	9.713ValAla: 9.713 ± 0.113	0.84ValCys: 0.84 ± 0.026	4.764ValAsp: 4.764 ± 0.063	4.413ValGlu: 4.413 ± 0.059	2.911ValPhe: 2.911 ± 0.056	5.907ValGly: 5.907 ± 0.067	1.663ValHis: 1.663 ± 0.035	3.667ValIle: 3.667 ± 0.058	2.157ValLys: 2.157 ± 0.051	8.119ValLeu: 8.119 ± 0.099	1.879ValMet: 1.879 ± 0.043	1.876ValAsn: 1.876 ± 0.042	3.872ValPro: 3.872 ± 0.054	2.125ValGln: 2.125 ± 0.039	5.498ValArg: 5.498 ± 0.084	4.38ValSer: 4.38 ± 0.08	4.16ValThr: 4.16 ± 0.059	6.402ValVal: 6.402 ± 0.081	0.998ValTrp: 0.998 ± 0.033	1.407ValTyr: 1.407 ± 0.03	0.0ValXaa: 0.0 ± 0.0
Trp	1.299TrpAla: 1.299 ± 0.038	0.189TrpCys: 0.189 ± 0.013	0.6TrpAsp: 0.6 ± 0.021	0.582TrpGlu: 0.582 ± 0.023	0.543TrpPhe: 0.543 ± 0.023	0.874TrpGly: 0.874 ± 0.031	0.458TrpHis: 0.458 ± 0.019	0.732TrpIle: 0.732 ± 0.024	0.438TrpLys: 0.438 ± 0.022	1.948TrpLeu: 1.948 ± 0.047	0.39TrpMet: 0.39 ± 0.018	0.34TrpAsn: 0.34 ± 0.016	0.695TrpPro: 0.695 ± 0.025	0.708TrpGln: 0.708 ± 0.025	1.283TrpArg: 1.283 ± 0.039	0.722TrpSer: 0.722 ± 0.025	0.661TrpThr: 0.661 ± 0.024	1.014TrpVal: 1.014 ± 0.029	0.243TrpTrp: 0.243 ± 0.015	0.278TrpTyr: 0.278 ± 0.016	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.538TyrAla: 2.538 ± 0.045	0.243TyrCys: 0.243 ± 0.014	1.339TyrAsp: 1.339 ± 0.038	1.114TyrGlu: 1.114 ± 0.033	0.906TyrPhe: 0.906 ± 0.027	1.908TyrGly: 1.908 ± 0.039	0.57TyrHis: 0.57 ± 0.022	0.747TyrIle: 0.747 ± 0.024	0.567TyrLys: 0.567 ± 0.022	2.437TyrLeu: 2.437 ± 0.044	0.413TyrMet: 0.413 ± 0.02	0.562TyrAsn: 0.562 ± 0.024	1.195TyrPro: 1.195 ± 0.032	0.792TyrGln: 0.792 ± 0.025	1.982TyrArg: 1.982 ± 0.047	1.056TyrSer: 1.056 ± 0.033	1.214TyrThr: 1.214 ± 0.068	1.724TyrVal: 1.724 ± 0.036	0.326TyrTrp: 0.326 ± 0.018	0.615TyrTyr: 0.615 ± 0.023	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3806 proteins (1265655 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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