bacterium AM6

Taxonomy: cellular organisms; Bacteria; unclassified Bacteria

Average proteome isoelectric point is 6.69

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2443 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A244C4N4|A0A244C4N4_9BACT Carboxymuconolactone decarboxylase family protein OS=bacterium AM6 OX=1954209 GN=B0X78_02875 PE=4 SV=1
MM1 pKa = 7.41LAVTLVLIALVLGLNLVASFVVLGRR26 pKa = 11.84DD27 pKa = 3.63DD28 pKa = 5.25LPASSRR34 pKa = 11.84AGQLLVVWLLPLVGAIVCMAMATADD59 pKa = 3.88GSPARR64 pKa = 11.84ADD66 pKa = 3.61GSGGGPADD74 pKa = 3.68FSGGYY79 pKa = 9.87SGDD82 pKa = 4.04SNSHH86 pKa = 6.05HH87 pKa = 7.19SYY89 pKa = 10.86SSSDD93 pKa = 3.58CSSGDD98 pKa = 3.28SGGGGDD104 pKa = 4.71GGSCGGGDD112 pKa = 3.22

Molecular weight: 10.76 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A244C399|A0A244C399_9BACT LysR family transcriptional regulator OS=bacterium AM6 OX=1954209 GN=B0X78_07990 PE=3 SV=1
RR1 pKa = 7.66KK2 pKa = 9.88VVGKK6 pKa = 10.23KK7 pKa = 9.83AATAKK12 pKa = 10.43KK13 pKa = 10.32VVGKK17 pKa = 8.64KK18 pKa = 7.51TAVAKK23 pKa = 10.36KK24 pKa = 9.51VAGKK28 pKa = 10.06KK29 pKa = 8.87VAATRR34 pKa = 11.84KK35 pKa = 7.28TVAKK39 pKa = 10.07KK40 pKa = 9.95AAPLKK45 pKa = 10.6KK46 pKa = 10.11AVAKK50 pKa = 9.98KK51 pKa = 10.51APAKK55 pKa = 10.07KK56 pKa = 9.98AVRR59 pKa = 11.84KK60 pKa = 8.38AAKK63 pKa = 9.9RR64 pKa = 11.84KK65 pKa = 9.33

Molecular weight: 6.74 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2443

0

2443

601141

30

1228

246.1

26.73

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.886 ± 0.081

0.908 ± 0.016

5.705 ± 0.039

5.056 ± 0.054

3.317 ± 0.033

8.498 ± 0.067

2.279 ± 0.027

4.042 ± 0.037

2.818 ± 0.049

11.002 ± 0.069

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.268 ± 0.027

2.604 ± 0.038

5.264 ± 0.037

4.377 ± 0.044

7.377 ± 0.047

5.392 ± 0.04

4.93 ± 0.046

7.457 ± 0.04

1.58 ± 0.025

2.24 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski