Sanxia water strider virus 20

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 7.12

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A1L3KF97|A0A1L3KF97_9VIRU RNA-directed RNA polymerase OS=Sanxia water strider virus 20 OX=1923404 PE=4 SV=1
MM1 pKa = 8.0ADD3 pKa = 3.78PHH5 pKa = 6.71PLPPDD10 pKa = 3.44LRR12 pKa = 11.84SKK14 pKa = 10.1GTVLRR19 pKa = 11.84EE20 pKa = 3.64EE21 pKa = 4.61TSCHH25 pKa = 5.58NGKK28 pKa = 7.64PTPAQQKK35 pKa = 7.41TVNQLNSLITNVVAAPSSPSVSTSPKK61 pKa = 9.49ASPVPGPLPASRR73 pKa = 11.84PVPQVYY79 pKa = 9.63IPKK82 pKa = 8.02TTQPRR87 pKa = 11.84PMTTADD93 pKa = 3.33YY94 pKa = 10.47MNFEE98 pKa = 4.28TSITIDD104 pKa = 4.24DD105 pKa = 5.02LPPLLPRR112 pKa = 11.84PPAGKK117 pKa = 10.04EE118 pKa = 3.52PAKK121 pKa = 10.55QPEE124 pKa = 4.56FVPAVPDD131 pKa = 4.3FSKK134 pKa = 11.0FSTEE138 pKa = 3.66MSKK141 pKa = 9.93MDD143 pKa = 3.04IVQRR147 pKa = 11.84KK148 pKa = 7.53VFMAQKK154 pKa = 10.18NKK156 pKa = 8.77EE157 pKa = 4.25HH158 pKa = 6.91NDD160 pKa = 3.12LLRR163 pKa = 11.84IYY165 pKa = 8.9NQQYY169 pKa = 8.69AQYY172 pKa = 10.32LAEE175 pKa = 5.06HH176 pKa = 6.98PNATDD181 pKa = 3.6FRR183 pKa = 11.84GRR185 pKa = 11.84NPQPILPQGIPQPISSPALTPGLNSTLLAKK215 pKa = 10.35DD216 pKa = 3.54PPPRR220 pKa = 11.84ASAGHH225 pKa = 6.73ALYY228 pKa = 10.2IPKK231 pKa = 10.29DD232 pKa = 3.12ILARR236 pKa = 11.84EE237 pKa = 4.52GSTTSGPSALTSIAPAAPEE256 pKa = 4.21PMSDD260 pKa = 3.58DD261 pKa = 3.88KK262 pKa = 11.56IFTGRR267 pKa = 11.84VNEE270 pKa = 4.97SITTLYY276 pKa = 10.73KK277 pKa = 10.47HH278 pKa = 6.12VFTDD282 pKa = 5.98GIHH285 pKa = 5.96EE286 pKa = 4.25RR287 pKa = 11.84LNIQGCEE294 pKa = 3.95DD295 pKa = 3.96GATKK299 pKa = 10.48LARR302 pKa = 11.84AILLAVDD309 pKa = 3.79SPVARR314 pKa = 11.84DD315 pKa = 3.49CGSRR319 pKa = 11.84QQPDD323 pKa = 3.24AAVDD327 pKa = 3.47ALYY330 pKa = 11.55AMAPTSADD338 pKa = 3.11SYY340 pKa = 9.78GTRR343 pKa = 11.84TTYY346 pKa = 10.53RR347 pKa = 11.84RR348 pKa = 11.84VDD350 pKa = 3.34NGVDD354 pKa = 2.99ANGRR358 pKa = 11.84QKK360 pKa = 9.51TRR362 pKa = 11.84EE363 pKa = 3.9VTEE366 pKa = 3.85TRR368 pKa = 11.84TVRR371 pKa = 11.84KK372 pKa = 10.13GSLTKK377 pKa = 10.48SANDD381 pKa = 3.32LAARR385 pKa = 11.84LIDD388 pKa = 3.97GNNNGYY394 pKa = 10.41RR395 pKa = 11.84IANFFSRR402 pKa = 11.84SSSNRR407 pKa = 11.84LFAVAVARR415 pKa = 11.84ALLRR419 pKa = 11.84KK420 pKa = 9.31KK421 pKa = 10.49ISVALYY427 pKa = 10.65SKK429 pKa = 10.74GLNSASKK436 pKa = 10.63ARR438 pKa = 11.84ALISAIQAYY447 pKa = 9.72AADD450 pKa = 4.14AVEE453 pKa = 4.62TTSPTLDD460 pKa = 3.01TSTRR464 pKa = 11.84DD465 pKa = 3.24IPDD468 pKa = 4.02AEE470 pKa = 4.24TLPRR474 pKa = 11.84SILPEE479 pKa = 4.78PIEE482 pKa = 4.41SMSASLAYY490 pKa = 10.24SRR492 pKa = 11.84LIAAKK497 pKa = 10.45LIDD500 pKa = 4.43LRR502 pKa = 11.84ACPVCVACAILGEE515 pKa = 4.27DD516 pKa = 3.43VPAEE520 pKa = 4.26VEE522 pKa = 4.27TPTATKK528 pKa = 10.48QNLRR532 pKa = 11.84EE533 pKa = 3.98QPPTSISKK541 pKa = 10.31LRR543 pKa = 11.84TLLSNGNSSRR553 pKa = 11.84FLEE556 pKa = 4.65AVANAHH562 pKa = 5.09NRR564 pKa = 11.84EE565 pKa = 3.96MHH567 pKa = 6.22SLNGNISFIVGLEE580 pKa = 3.87EE581 pKa = 4.69PRR583 pKa = 11.84PQVIQRR589 pKa = 11.84CIIEE593 pKa = 4.53TVQPPVKK600 pKa = 10.75VPVTSFRR607 pKa = 11.84DD608 pKa = 3.31DD609 pKa = 3.62AYY611 pKa = 11.48NILQQKK617 pKa = 6.04TTDD620 pKa = 3.49EE621 pKa = 4.01LTLRR625 pKa = 11.84VGDD628 pKa = 3.77GRR630 pKa = 11.84STITLQPAPTLHH642 pKa = 6.37QLVLNLDD649 pKa = 3.77PAARR653 pKa = 11.84GPMLDD658 pKa = 4.0AGNNMTIPPRR668 pKa = 11.84MRR670 pKa = 11.84DD671 pKa = 3.72NQPSTSSSDD680 pKa = 3.52APPPAQAGKK689 pKa = 10.89GKK691 pKa = 9.19ATVRR695 pKa = 11.84NASKK699 pKa = 9.55TRR701 pKa = 11.84AQTDD705 pKa = 3.35KK706 pKa = 10.9APEE709 pKa = 4.21VFSEE713 pKa = 4.72PPTQTSSSNSTPDD726 pKa = 3.25PRR728 pKa = 11.84KK729 pKa = 7.88TSVLGRR735 pKa = 11.84IRR737 pKa = 11.84KK738 pKa = 7.44VFKK741 pKa = 11.09VIDD744 pKa = 4.1KK745 pKa = 10.99VPATCKK751 pKa = 9.34TNRR754 pKa = 11.84PSTILGDD761 pKa = 4.03APDD764 pKa = 3.98LSGLGTSSSLGDD776 pKa = 3.31SDD778 pKa = 5.83SFISYY783 pKa = 9.47PDD785 pKa = 2.92IMAAAGPAYY794 pKa = 10.19VPPLYY799 pKa = 10.3IGVGQSADD807 pKa = 3.22VGGGHH812 pKa = 7.05FFLNPVATDD821 pKa = 3.39FLARR825 pKa = 11.84VRR827 pKa = 11.84DD828 pKa = 3.8ADD830 pKa = 3.8TKK832 pKa = 11.08EE833 pKa = 3.75PGIACRR839 pKa = 11.84LPEE842 pKa = 4.75NILGRR847 pKa = 11.84LANAPYY853 pKa = 10.66LLSALANTLPSDD865 pKa = 3.73NSDD868 pKa = 3.18FTARR872 pKa = 11.84TLKK875 pKa = 10.22VSLYY879 pKa = 9.82EE880 pKa = 4.05GLRR883 pKa = 11.84EE884 pKa = 3.95MRR886 pKa = 11.84NTNPLGVAFAAPLNAYY902 pKa = 7.93PIRR905 pKa = 11.84EE906 pKa = 4.19NIALNQQQDD915 pKa = 2.74WQYY918 pKa = 9.06RR919 pKa = 11.84HH920 pKa = 6.67IIVSYY925 pKa = 10.44SYY927 pKa = 11.25LEE929 pKa = 3.69EE930 pKa = 4.33TIRR933 pKa = 11.84LGAEE937 pKa = 3.74IPVAGQDD944 pKa = 3.37PWSLKK949 pKa = 10.43AQDD952 pKa = 3.9TVVVCLDD959 pKa = 3.48YY960 pKa = 11.48DD961 pKa = 3.75AVDD964 pKa = 4.0TNVVWALQMLTALPYY979 pKa = 9.11PLLWVAEE986 pKa = 4.05WQAYY990 pKa = 8.46LRR992 pKa = 11.84QRR994 pKa = 11.84NDD996 pKa = 3.15DD997 pKa = 3.69PTDD1000 pKa = 3.57VFWTLGQFLRR1010 pKa = 11.84NDD1012 pKa = 3.8GLVSLNDD1019 pKa = 3.46MVKK1022 pKa = 10.35KK1023 pKa = 10.7VIFVTSTMLPQITISGHH1040 pKa = 6.5LYY1042 pKa = 9.7PVTVSLGPRR1051 pKa = 11.84VPLPPAQVYY1060 pKa = 10.48DD1061 pKa = 3.93SEE1063 pKa = 4.82ALILSSLTLILASVTPLRR1081 pKa = 11.84KK1082 pKa = 8.87IWEE1085 pKa = 4.52SLWSQRR1091 pKa = 11.84LGGSALNWTRR1101 pKa = 11.84IEE1103 pKa = 4.21NYY1105 pKa = 9.33VAYY1108 pKa = 8.86LTTRR1112 pKa = 11.84WHH1114 pKa = 6.59PQLEE1118 pKa = 4.47MATGGDD1124 pKa = 4.21GVPHH1128 pKa = 5.99SFWWQGPLPDD1138 pKa = 4.15NSPILFEE1145 pKa = 4.76MGDD1148 pKa = 3.86ADD1150 pKa = 4.51TLFATDD1156 pKa = 4.63AVTAGDD1162 pKa = 4.3NIFLPRR1168 pKa = 11.84PDD1170 pKa = 4.9ADD1172 pKa = 3.47INPAMRR1178 pKa = 11.84LGRR1181 pKa = 11.84WTNWAEE1187 pKa = 3.79LAVGLRR1193 pKa = 11.84VASYY1197 pKa = 10.61RR1198 pKa = 11.84SEE1200 pKa = 4.03SLNLQQLDD1208 pKa = 3.63PHH1210 pKa = 7.65IDD1212 pKa = 3.09SSGYY1216 pKa = 10.5SRR1218 pKa = 11.84LTVAKK1223 pKa = 8.49FQRR1226 pKa = 11.84RR1227 pKa = 11.84AIEE1230 pKa = 3.78EE1231 pKa = 4.13WKK1233 pKa = 10.73YY1234 pKa = 11.55SSLTSDD1240 pKa = 4.35EE1241 pKa = 3.98IVAPHH1246 pKa = 7.4AYY1248 pKa = 10.25NLLGGNWNKK1257 pKa = 10.08LVYY1260 pKa = 10.69GDD1262 pKa = 4.93GSTKK1266 pKa = 10.25QLFFSLLVARR1276 pKa = 11.84ILGLTPSDD1284 pKa = 3.41GTMNWSGNWMRR1295 pKa = 11.84AVNQTFTGIRR1305 pKa = 11.84TSNAMWLDD1313 pKa = 3.57EE1314 pKa = 4.2RR1315 pKa = 11.84SGDD1318 pKa = 3.85LTCTITDD1325 pKa = 3.36TSLTYY1330 pKa = 9.18ITNLNDD1336 pKa = 3.64PLYY1339 pKa = 9.91QFWVQAQNPKK1349 pKa = 10.93DD1350 pKa = 3.95DD1351 pKa = 3.87VTLSRR1356 pKa = 11.84LLNRR1360 pKa = 11.84LDD1362 pKa = 5.11LLQQHH1367 pKa = 6.23TALQSKK1373 pKa = 8.12VWPQAALLPINMVRR1387 pKa = 11.84LEE1389 pKa = 4.38TTTFTALNILAGNNLLLNHH1408 pKa = 6.77PTTPLEE1414 pKa = 4.09MPIGWSYY1421 pKa = 11.46GSPILQKK1428 pKa = 10.77DD1429 pKa = 3.11WTQFRR1434 pKa = 11.84FLSVAVSAAAQRR1446 pKa = 11.84SLEE1449 pKa = 4.18TRR1451 pKa = 11.84TSSPGTTLRR1460 pKa = 11.84WPGVLIKK1467 pKa = 10.64SEE1469 pKa = 4.25GSAATYY1475 pKa = 10.55LEE1477 pKa = 4.63HH1478 pKa = 7.96DD1479 pKa = 3.59WTLLADD1485 pKa = 4.03EE1486 pKa = 4.46VTAMGGTPAGVTSSSDD1502 pKa = 3.08SSVFRR1507 pKa = 11.84LGPSSTDD1514 pKa = 2.83RR1515 pKa = 11.84GPPPDD1520 pKa = 3.95GVTSSGQEE1528 pKa = 3.76NPMVQPPGSDD1538 pKa = 3.44DD1539 pKa = 3.43ARR1541 pKa = 11.84LGAEE1545 pKa = 4.28GLSNHH1550 pKa = 7.13PDD1552 pKa = 3.33HH1553 pKa = 6.85NQDD1556 pKa = 4.03RR1557 pKa = 11.84PQIDD1561 pKa = 2.98

Molecular weight:
170.13 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A1L3KF97|A0A1L3KF97_9VIRU RNA-directed RNA polymerase OS=Sanxia water strider virus 20 OX=1923404 PE=4 SV=1
MM1 pKa = 7.27TKK3 pKa = 10.32VNAIIDD9 pKa = 3.97DD10 pKa = 4.09SPVCKK15 pKa = 10.47EE16 pKa = 3.88SDD18 pKa = 3.92CVGGSVWTQPTSSFLLNIRR37 pKa = 11.84RR38 pKa = 11.84YY39 pKa = 9.73RR40 pKa = 11.84FHH42 pKa = 7.48EE43 pKa = 3.84PHH45 pKa = 6.44SCQACPLFKK54 pKa = 10.52INNAVEE60 pKa = 4.59SFFHH64 pKa = 7.63LMFFTLAPNIVSTIMPFFQFIRR86 pKa = 11.84KK87 pKa = 7.25RR88 pKa = 11.84TQGFCQHH95 pKa = 5.79VFRR98 pKa = 11.84KK99 pKa = 9.4VLKK102 pKa = 10.5AVTGLVKK109 pKa = 10.82LGGPYY114 pKa = 8.22ITKK117 pKa = 9.33SWRR120 pKa = 11.84YY121 pKa = 7.63LCYY124 pKa = 9.61WEE126 pKa = 5.68ASLGYY131 pKa = 8.56TPYY134 pKa = 10.82RR135 pKa = 11.84PLSDD139 pKa = 4.54LKK141 pKa = 10.81PDD143 pKa = 3.46VEE145 pKa = 4.15AWLVNPHH152 pKa = 6.42INGEE156 pKa = 4.26GMGTDD161 pKa = 3.46VFHH164 pKa = 7.3HH165 pKa = 6.63KK166 pKa = 10.61LYY168 pKa = 10.96DD169 pKa = 3.54ATCEE173 pKa = 3.98FLRR176 pKa = 11.84KK177 pKa = 8.15EE178 pKa = 4.4WKK180 pKa = 10.13RR181 pKa = 11.84PSNPPTLKK189 pKa = 10.04QWLEE193 pKa = 3.5KK194 pKa = 9.64AAWMRR199 pKa = 11.84GRR201 pKa = 11.84GGTGNKK207 pKa = 8.18STILIDD213 pKa = 3.44NKK215 pKa = 10.3RR216 pKa = 11.84VKK218 pKa = 9.43TRR220 pKa = 11.84SNKK223 pKa = 9.74GVDD226 pKa = 3.66AIFLNDD232 pKa = 3.15EE233 pKa = 3.91ALARR237 pKa = 11.84EE238 pKa = 4.55MFTVIPQKK246 pKa = 10.16MVIMQKK252 pKa = 9.88SEE254 pKa = 3.76PAKK257 pKa = 9.93IRR259 pKa = 11.84PVAKK263 pKa = 10.26ADD265 pKa = 3.44NQLFRR270 pKa = 11.84KK271 pKa = 9.01MDD273 pKa = 3.8FLSEE277 pKa = 3.96IVEE280 pKa = 4.34FGLMGSKK287 pKa = 10.21RR288 pKa = 11.84SSLFLGPVGNEE299 pKa = 3.42RR300 pKa = 11.84LDD302 pKa = 3.72LHH304 pKa = 7.96ILDD307 pKa = 5.66HH308 pKa = 6.3IQHH311 pKa = 7.32GINVPLDD318 pKa = 3.19QGSFDD323 pKa = 4.13NNQGKK328 pKa = 9.33PSILVVLLAIYY339 pKa = 8.72DD340 pKa = 3.52VCLAQEE346 pKa = 4.35EE347 pKa = 4.21NHH349 pKa = 6.65EE350 pKa = 4.2YY351 pKa = 9.85QKK353 pKa = 10.96VWAAMWDD360 pKa = 4.08SFVHH364 pKa = 6.62PDD366 pKa = 3.04SKK368 pKa = 11.43VILGEE373 pKa = 5.72DD374 pKa = 3.38SFPWHH379 pKa = 6.61NGVASGWRR387 pKa = 11.84WTAFIDD393 pKa = 4.24TILNIATYY401 pKa = 10.6DD402 pKa = 3.54VLTKK406 pKa = 10.69YY407 pKa = 10.52VSQYY411 pKa = 10.33TGATFYY417 pKa = 11.42DD418 pKa = 4.44SVHH421 pKa = 6.55QGDD424 pKa = 5.27DD425 pKa = 3.0IHH427 pKa = 6.74FKK429 pKa = 10.84CDD431 pKa = 3.22TLGGVQTLINAYY443 pKa = 9.18KK444 pKa = 9.77DD445 pKa = 3.11NGYY448 pKa = 9.55EE449 pKa = 3.85VHH451 pKa = 7.04ASKK454 pKa = 9.67TYY456 pKa = 10.74ASTTRR461 pKa = 11.84TEE463 pKa = 4.04FLRR466 pKa = 11.84RR467 pKa = 11.84SYY469 pKa = 10.21EE470 pKa = 3.78KK471 pKa = 10.84CGVLGYY477 pKa = 9.06YY478 pKa = 10.01CRR480 pKa = 11.84SLQGLLYY487 pKa = 10.16RR488 pKa = 11.84NPITEE493 pKa = 4.0QTILPASRR501 pKa = 11.84IYY503 pKa = 11.12DD504 pKa = 4.53RR505 pKa = 11.84ITVALLMVLRR515 pKa = 11.84GGSPRR520 pKa = 11.84GIAQWIRR527 pKa = 11.84EE528 pKa = 4.39DD529 pKa = 3.68ASQLHH534 pKa = 5.94VSDD537 pKa = 5.25PEE539 pKa = 4.07LSDD542 pKa = 5.8FILTPSAVGGVGMSGASGPLASEE565 pKa = 4.6FLSTATGMWTIPRR578 pKa = 11.84SEE580 pKa = 4.02EE581 pKa = 3.69TSRR584 pKa = 11.84PRR586 pKa = 11.84CIVDD590 pKa = 3.59LGQWNGRR597 pKa = 11.84LQRR600 pKa = 11.84VGVILDD606 pKa = 3.26QTNRR610 pKa = 11.84NNFLNDD616 pKa = 3.71LVRR619 pKa = 11.84TWGIRR624 pKa = 11.84TTDD627 pKa = 3.19VSGDD631 pKa = 3.47FSFFWEE637 pKa = 4.67KK638 pKa = 10.42IKK640 pKa = 10.8PFAPLPITGGMDD652 pKa = 4.09LPPPSDD658 pKa = 2.32IWADD662 pKa = 3.48EE663 pKa = 4.17KK664 pKa = 11.41VPTMLRR670 pKa = 11.84QHH672 pKa = 7.16WKK674 pKa = 9.18NQLIKK679 pKa = 10.94DD680 pKa = 4.21GTWTNHH686 pKa = 5.0VKK688 pKa = 10.41PEE690 pKa = 4.04HH691 pKa = 6.57LEE693 pKa = 3.61QLKK696 pKa = 10.41RR697 pKa = 11.84LRR699 pKa = 11.84KK700 pKa = 9.61RR701 pKa = 11.84ISAGLFEE708 pKa = 6.46DD709 pKa = 4.24YY710 pKa = 11.33LLGTVRR716 pKa = 11.84EE717 pKa = 4.23PVPVVDD723 pKa = 6.2GVALKK728 pKa = 10.7YY729 pKa = 10.02GQGWKK734 pKa = 9.11QQAKK738 pKa = 7.51QWMLRR743 pKa = 11.84ALSSNLCDD751 pKa = 3.84RR752 pKa = 11.84KK753 pKa = 10.31IFEE756 pKa = 5.65KK757 pKa = 10.82KK758 pKa = 9.71MLWLEE763 pKa = 4.04SLAIRR768 pKa = 11.84HH769 pKa = 6.01ISRR772 pKa = 11.84LRR774 pKa = 11.84LFGTLALL781 pKa = 4.43

Molecular weight:
88.9 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

2342

781

1561

1171.0

129.51

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.07 ± 1.434

1.025 ± 0.358

5.892 ± 0.216

4.056 ± 0.309

3.202 ± 0.858

6.191 ± 0.547

1.836 ± 0.477

5.124 ± 0.428

4.184 ± 0.954

10.205 ± 0.122

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.964 ± 0.19

4.611 ± 0.359

7.43 ± 1.291

4.184 ± 0.12

6.02 ± 0.07

7.857 ± 0.742

7.301 ± 0.789

6.106 ± 0.237

2.05 ± 0.501

2.69 ± 0.143

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski