Amino acid dipepetide frequency for Microcella sp. HL-107

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
19.221AlaAla: 19.221 ± 0.284
0.554AlaCys: 0.554 ± 0.031
7.684AlaAsp: 7.684 ± 0.117
8.793AlaGlu: 8.793 ± 0.142
3.517AlaPhe: 3.517 ± 0.07
11.313AlaGly: 11.313 ± 0.164
2.717AlaHis: 2.717 ± 0.07
6.105AlaIle: 6.105 ± 0.097
2.254AlaLys: 2.254 ± 0.076
15.145AlaLeu: 15.145 ± 0.213
2.46AlaMet: 2.46 ± 0.057
2.064AlaAsn: 2.064 ± 0.06
6.593AlaPro: 6.593 ± 0.118
4.041AlaGln: 4.041 ± 0.083
9.771AlaArg: 9.771 ± 0.151
7.407AlaSer: 7.407 ± 0.138
7.513AlaThr: 7.513 ± 0.119
11.506AlaVal: 11.506 ± 0.161
1.72AlaTrp: 1.72 ± 0.054
2.263AlaTyr: 2.263 ± 0.071
0.0AlaXaa: 0.0 ± 0.0
Cys
0.608CysAla: 0.608 ± 0.032
0.044CysCys: 0.044 ± 0.008
0.279CysAsp: 0.279 ± 0.019
0.292CysGlu: 0.292 ± 0.029
0.123CysPhe: 0.123 ± 0.013
0.53CysGly: 0.53 ± 0.028
0.102CysHis: 0.102 ± 0.012
0.18CysIle: 0.18 ± 0.017
0.048CysLys: 0.048 ± 0.008
0.421CysLeu: 0.421 ± 0.026
0.071CysMet: 0.071 ± 0.01
0.099CysAsn: 0.099 ± 0.013
0.276CysPro: 0.276 ± 0.019
0.118CysGln: 0.118 ± 0.015
0.347CysArg: 0.347 ± 0.023
0.299CysSer: 0.299 ± 0.02
0.315CysThr: 0.315 ± 0.022
0.422CysVal: 0.422 ± 0.03
0.055CysTrp: 0.055 ± 0.009
0.094CysTyr: 0.094 ± 0.013
0.0CysXaa: 0.0 ± 0.0
Asp
8.774AspAla: 8.774 ± 0.118
0.289AspCys: 0.289 ± 0.029
4.873AspAsp: 4.873 ± 0.103
4.475AspGlu: 4.475 ± 0.084
1.676AspPhe: 1.676 ± 0.053
5.548AspGly: 5.548 ± 0.095
1.263AspHis: 1.263 ± 0.046
2.347AspIle: 2.347 ± 0.058
0.864AspLys: 0.864 ± 0.043
6.297AspLeu: 6.297 ± 0.108
0.755AspMet: 0.755 ± 0.033
0.996AspAsn: 0.996 ± 0.042
3.992AspPro: 3.992 ± 0.076
1.582AspGln: 1.582 ± 0.048
4.593AspArg: 4.593 ± 0.087
2.883AspSer: 2.883 ± 0.074
3.042AspThr: 3.042 ± 0.076
5.118AspVal: 5.118 ± 0.083
0.868AspTrp: 0.868 ± 0.037
1.306AspTyr: 1.306 ± 0.05
0.0AspXaa: 0.0 ± 0.0
Glu
7.332GluAla: 7.332 ± 0.116
0.277GluCys: 0.277 ± 0.023
2.238GluAsp: 2.238 ± 0.067
2.973GluGlu: 2.973 ± 0.078
1.811GluPhe: 1.811 ± 0.049
4.831GluGly: 4.831 ± 0.085
1.676GluHis: 1.676 ± 0.06
2.666GluIle: 2.666 ± 0.076
1.422GluLys: 1.422 ± 0.062
7.356GluLeu: 7.356 ± 0.119
0.824GluMet: 0.824 ± 0.039
1.238GluAsn: 1.238 ± 0.048
3.516GluPro: 3.516 ± 0.077
2.478GluGln: 2.478 ± 0.052
5.568GluArg: 5.568 ± 0.113
3.383GluSer: 3.383 ± 0.068
3.082GluThr: 3.082 ± 0.073
5.003GluVal: 5.003 ± 0.096
0.926GluTrp: 0.926 ± 0.039
1.132GluTyr: 1.132 ± 0.04
0.0GluXaa: 0.0 ± 0.0
Phe
3.993PheAla: 3.993 ± 0.07
0.184PheCys: 0.184 ± 0.016
2.276PheAsp: 2.276 ± 0.051
1.735PheGlu: 1.735 ± 0.054
1.004PhePhe: 1.004 ± 0.044
3.259PheGly: 3.259 ± 0.076
0.511PheHis: 0.511 ± 0.028
1.131PheIle: 1.131 ± 0.044
0.373PheLys: 0.373 ± 0.023
2.727PheLeu: 2.727 ± 0.072
0.399PheMet: 0.399 ± 0.023
0.524PheAsn: 0.524 ± 0.033
1.305PhePro: 1.305 ± 0.048
0.591PheGln: 0.591 ± 0.032
1.713PheArg: 1.713 ± 0.048
1.656PheSer: 1.656 ± 0.048
2.126PheThr: 2.126 ± 0.074
2.739PheVal: 2.739 ± 0.068
0.415PheTrp: 0.415 ± 0.024
0.62PheTyr: 0.62 ± 0.03
0.0PheXaa: 0.0 ± 0.0
Gly
10.83GlyAla: 10.83 ± 0.151
0.508GlyCys: 0.508 ± 0.028
5.148GlyAsp: 5.148 ± 0.109
5.22GlyGlu: 5.22 ± 0.096
3.108GlyPhe: 3.108 ± 0.073
7.646GlyGly: 7.646 ± 0.144
1.854GlyHis: 1.854 ± 0.067
4.774GlyIle: 4.774 ± 0.1
1.875GlyLys: 1.875 ± 0.058
9.009GlyLeu: 9.009 ± 0.136
1.875GlyMet: 1.875 ± 0.053
1.598GlyAsn: 1.598 ± 0.052
3.73GlyPro: 3.73 ± 0.07
2.562GlyGln: 2.562 ± 0.068
6.125GlyArg: 6.125 ± 0.1
5.394GlySer: 5.394 ± 0.101
5.269GlyThr: 5.269 ± 0.088
8.021GlyVal: 8.021 ± 0.127
1.408GlyTrp: 1.408 ± 0.052
2.084GlyTyr: 2.084 ± 0.054
0.0GlyXaa: 0.0 ± 0.0
His
2.588HisAla: 2.588 ± 0.068
0.113HisCys: 0.113 ± 0.012
1.43HisAsp: 1.43 ± 0.046
1.364HisGlu: 1.364 ± 0.046
0.514HisPhe: 0.514 ± 0.028
2.039HisGly: 2.039 ± 0.065
0.624HisHis: 0.624 ± 0.032
0.739HisIle: 0.739 ± 0.038
0.269HisLys: 0.269 ± 0.021
1.999HisLeu: 1.999 ± 0.061
0.298HisMet: 0.298 ± 0.022
0.347HisAsn: 0.347 ± 0.024
1.507HisPro: 1.507 ± 0.049
0.517HisGln: 0.517 ± 0.029
1.729HisArg: 1.729 ± 0.056
1.035HisSer: 1.035 ± 0.044
0.986HisThr: 0.986 ± 0.038
1.6HisVal: 1.6 ± 0.049
0.279HisTrp: 0.279 ± 0.02
0.48HisTyr: 0.48 ± 0.027
0.0HisXaa: 0.0 ± 0.0
Ile
7.072IleAla: 7.072 ± 0.107
0.202IleCys: 0.202 ± 0.018
3.53IleAsp: 3.53 ± 0.073
3.039IleGlu: 3.039 ± 0.066
1.115IlePhe: 1.115 ± 0.047
4.442IleGly: 4.442 ± 0.09
0.675IleHis: 0.675 ± 0.033
2.167IleIle: 2.167 ± 0.07
0.72IleLys: 0.72 ± 0.039
3.839IleLeu: 3.839 ± 0.08
0.695IleMet: 0.695 ± 0.036
0.926IleAsn: 0.926 ± 0.04
2.349IlePro: 2.349 ± 0.06
0.88IleGln: 0.88 ± 0.037
2.592IleArg: 2.592 ± 0.068
2.237IleSer: 2.237 ± 0.056
3.08IleThr: 3.08 ± 0.072
5.021IleVal: 5.021 ± 0.085
0.396IleTrp: 0.396 ± 0.025
0.742IleTyr: 0.742 ± 0.031
0.0IleXaa: 0.0 ± 0.0
Lys
2.074LysAla: 2.074 ± 0.085
0.055LysCys: 0.055 ± 0.009
0.92LysAsp: 0.92 ± 0.038
0.832LysGlu: 0.832 ± 0.044
0.435LysPhe: 0.435 ± 0.027
1.357LysGly: 1.357 ± 0.052
0.424LysHis: 0.424 ± 0.028
0.771LysIle: 0.771 ± 0.037
0.787LysLys: 0.787 ± 0.057
1.729LysLeu: 1.729 ± 0.062
0.329LysMet: 0.329 ± 0.024
0.504LysAsn: 0.504 ± 0.032
1.096LysPro: 1.096 ± 0.052
0.617LysGln: 0.617 ± 0.034
1.549LysArg: 1.549 ± 0.058
0.996LysSer: 0.996 ± 0.045
1.199LysThr: 1.199 ± 0.054
1.457LysVal: 1.457 ± 0.056
0.192LysTrp: 0.192 ± 0.016
0.389LysTyr: 0.389 ± 0.026
0.0LysXaa: 0.0 ± 0.0
Leu
15.48LeuAla: 15.48 ± 0.2
0.469LeuCys: 0.469 ± 0.025
7.39LeuAsp: 7.39 ± 0.114
5.987LeuGlu: 5.987 ± 0.109
2.781LeuPhe: 2.781 ± 0.07
9.562LeuGly: 9.562 ± 0.157
2.002LeuHis: 2.002 ± 0.053
4.478LeuIle: 4.478 ± 0.097
1.55LeuLys: 1.55 ± 0.064
10.95LeuLeu: 10.95 ± 0.173
1.62LeuMet: 1.62 ± 0.053
1.816LeuAsn: 1.816 ± 0.061
5.577LeuPro: 5.577 ± 0.085
2.726LeuGln: 2.726 ± 0.061
7.559LeuArg: 7.559 ± 0.116
6.035LeuSer: 6.035 ± 0.102
6.802LeuThr: 6.802 ± 0.11
9.789LeuVal: 9.789 ± 0.151
1.315LeuTrp: 1.315 ± 0.044
1.623LeuTyr: 1.623 ± 0.047
0.0LeuXaa: 0.0 ± 0.0
Met
2.084MetAla: 2.084 ± 0.055
0.081MetCys: 0.081 ± 0.011
0.72MetAsp: 0.72 ± 0.031
0.66MetGlu: 0.66 ± 0.031
0.437MetPhe: 0.437 ± 0.024
1.272MetGly: 1.272 ± 0.044
0.322MetHis: 0.322 ± 0.023
0.91MetIle: 0.91 ± 0.038
0.379MetLys: 0.379 ± 0.023
1.912MetLeu: 1.912 ± 0.045
0.263MetMet: 0.263 ± 0.022
0.396MetAsn: 0.396 ± 0.027
1.064MetPro: 1.064 ± 0.037
0.48MetGln: 0.48 ± 0.028
1.346MetArg: 1.346 ± 0.038
1.346MetSer: 1.346 ± 0.04
1.769MetThr: 1.769 ± 0.045
1.366MetVal: 1.366 ± 0.044
0.174MetTrp: 0.174 ± 0.017
0.251MetTyr: 0.251 ± 0.02
0.0MetXaa: 0.0 ± 0.0
Asn
2.218AsnAla: 2.218 ± 0.063
0.134AsnCys: 0.134 ± 0.014
1.2AsnAsp: 1.2 ± 0.064
0.973AsnGlu: 0.973 ± 0.038
0.546AsnPhe: 0.546 ± 0.031
1.761AsnGly: 1.761 ± 0.055
0.318AsnHis: 0.318 ± 0.021
0.777AsnIle: 0.777 ± 0.037
0.331AsnLys: 0.331 ± 0.027
1.888AsnLeu: 1.888 ± 0.052
0.289AsnMet: 0.289 ± 0.021
0.476AsnAsn: 0.476 ± 0.031
1.573AsnPro: 1.573 ± 0.049
0.553AsnGln: 0.553 ± 0.031
1.379AsnArg: 1.379 ± 0.047
0.954AsnSer: 0.954 ± 0.039
1.013AsnThr: 1.013 ± 0.051
1.549AsnVal: 1.549 ± 0.047
0.279AsnTrp: 0.279 ± 0.019
0.45AsnTyr: 0.45 ± 0.026
0.0AsnXaa: 0.0 ± 0.0
Pro
7.191ProAla: 7.191 ± 0.126
0.179ProCys: 0.179 ± 0.015
3.889ProAsp: 3.889 ± 0.077
3.881ProGlu: 3.881 ± 0.089
1.581ProPhe: 1.581 ± 0.045
4.995ProGly: 4.995 ± 0.086
1.18ProHis: 1.18 ± 0.044
2.108ProIle: 2.108 ± 0.058
0.917ProLys: 0.917 ± 0.048
5.228ProLeu: 5.228 ± 0.099
0.885ProMet: 0.885 ± 0.036
1.004ProAsn: 1.004 ± 0.042
2.571ProPro: 2.571 ± 0.083
1.575ProGln: 1.575 ± 0.051
3.526ProArg: 3.526 ± 0.084
3.266ProSer: 3.266 ± 0.078
3.353ProThr: 3.353 ± 0.085
4.8ProVal: 4.8 ± 0.089
0.833ProTrp: 0.833 ± 0.032
1.007ProTyr: 1.007 ± 0.039
0.0ProXaa: 0.0 ± 0.0
Gln
3.25GlnAla: 3.25 ± 0.073
0.15GlnCys: 0.15 ± 0.017
1.029GlnAsp: 1.029 ± 0.038
1.301GlnGlu: 1.301 ± 0.048
0.858GlnPhe: 0.858 ± 0.037
2.093GlnGly: 2.093 ± 0.054
0.647GlnHis: 0.647 ± 0.032
1.299GlnIle: 1.299 ± 0.044
0.681GlnLys: 0.681 ± 0.033
3.472GlnLeu: 3.472 ± 0.078
0.505GlnMet: 0.505 ± 0.025
0.691GlnAsn: 0.691 ± 0.032
1.613GlnPro: 1.613 ± 0.05
1.308GlnGln: 1.308 ± 0.048
2.674GlnArg: 2.674 ± 0.078
1.662GlnSer: 1.662 ± 0.05
1.518GlnThr: 1.518 ± 0.045
2.475GlnVal: 2.475 ± 0.064
0.536GlnTrp: 0.536 ± 0.028
0.684GlnTyr: 0.684 ± 0.029
0.0GlnXaa: 0.0 ± 0.0
Arg
9.319ArgAla: 9.319 ± 0.148
0.332ArgCys: 0.332 ± 0.027
4.54ArgAsp: 4.54 ± 0.095
4.78ArgGlu: 4.78 ± 0.096
2.315ArgPhe: 2.315 ± 0.061
5.67ArgGly: 5.67 ± 0.094
1.579ArgHis: 1.579 ± 0.056
3.575ArgIle: 3.575 ± 0.073
1.177ArgLys: 1.177 ± 0.05
7.767ArgLeu: 7.767 ± 0.122
1.655ArgMet: 1.655 ± 0.051
1.253ArgAsn: 1.253 ± 0.046
3.675ArgPro: 3.675 ± 0.079
2.155ArgGln: 2.155 ± 0.057
6.546ArgArg: 6.546 ± 0.136
4.251ArgSer: 4.251 ± 0.102
4.244ArgThr: 4.244 ± 0.084
6.273ArgVal: 6.273 ± 0.1
1.241ArgTrp: 1.241 ± 0.045
1.571ArgTyr: 1.571 ± 0.052
0.0ArgXaa: 0.0 ± 0.0
Ser
7.385SerAla: 7.385 ± 0.131
0.263SerCys: 0.263 ± 0.023
3.14SerAsp: 3.14 ± 0.068
2.907SerGlu: 2.907 ± 0.069
1.79SerPhe: 1.79 ± 0.055
5.732SerGly: 5.732 ± 0.111
0.984SerHis: 0.984 ± 0.036
2.753SerIle: 2.753 ± 0.068
1.019SerLys: 1.019 ± 0.047
5.787SerLeu: 5.787 ± 0.11
1.212SerMet: 1.212 ± 0.04
1.044SerAsn: 1.044 ± 0.042
3.228SerPro: 3.228 ± 0.073
1.382SerGln: 1.382 ± 0.042
3.957SerArg: 3.957 ± 0.081
3.665SerSer: 3.665 ± 0.076
4.128SerThr: 4.128 ± 0.084
5.06SerVal: 5.06 ± 0.122
0.833SerTrp: 0.833 ± 0.038
1.107SerTyr: 1.107 ± 0.044
0.0SerXaa: 0.0 ± 0.0
Thr
7.686ThrAla: 7.686 ± 0.113
0.232ThrCys: 0.232 ± 0.02
3.462ThrAsp: 3.462 ± 0.084
3.414ThrGlu: 3.414 ± 0.076
1.713ThrPhe: 1.713 ± 0.056
5.729ThrGly: 5.729 ± 0.101
1.118ThrHis: 1.118 ± 0.046
3.006ThrIle: 3.006 ± 0.07
1.064ThrLys: 1.064 ± 0.042
6.514ThrLeu: 6.514 ± 0.103
1.036ThrMet: 1.036 ± 0.043
1.205ThrAsn: 1.205 ± 0.063
4.028ThrPro: 4.028 ± 0.086
1.536ThrGln: 1.536 ± 0.048
3.894ThrArg: 3.894 ± 0.092
3.402ThrSer: 3.402 ± 0.073
4.159ThrThr: 4.159 ± 0.097
6.5ThrVal: 6.5 ± 0.163
0.775ThrTrp: 0.775 ± 0.034
1.184ThrTyr: 1.184 ± 0.079
0.0ThrXaa: 0.0 ± 0.0
Val
11.698ValAla: 11.698 ± 0.156
0.435ValCys: 0.435 ± 0.024
5.961ValAsp: 5.961 ± 0.096
5.36ValGlu: 5.36 ± 0.089
2.7ValPhe: 2.7 ± 0.062
7.423ValGly: 7.423 ± 0.117
1.859ValHis: 1.859 ± 0.053
4.609ValIle: 4.609 ± 0.093
1.43ValLys: 1.43 ± 0.052
9.668ValLeu: 9.668 ± 0.156
1.508ValMet: 1.508 ± 0.05
1.649ValAsn: 1.649 ± 0.063
4.635ValPro: 4.635 ± 0.088
2.238ValGln: 2.238 ± 0.054
6.189ValArg: 6.189 ± 0.108
5.324ValSer: 5.324 ± 0.101
6.218ValThr: 6.218 ± 0.15
8.967ValVal: 8.967 ± 0.16
1.007ValTrp: 1.007 ± 0.04
1.483ValTyr: 1.483 ± 0.052
0.0ValXaa: 0.0 ± 0.0
Trp
1.528TrpAla: 1.528 ± 0.048
0.083TrpCys: 0.083 ± 0.011
0.585TrpAsp: 0.585 ± 0.03
0.599TrpGlu: 0.599 ± 0.025
0.544TrpPhe: 0.544 ± 0.028
0.99TrpGly: 0.99 ± 0.035
0.329TrpHis: 0.329 ± 0.02
0.639TrpIle: 0.639 ± 0.031
0.264TrpLys: 0.264 ± 0.022
1.624TrpLeu: 1.624 ± 0.052
0.343TrpMet: 0.343 ± 0.023
0.405TrpAsn: 0.405 ± 0.023
0.691TrpPro: 0.691 ± 0.029
0.585TrpGln: 0.585 ± 0.03
1.19TrpArg: 1.19 ± 0.045
1.019TrpSer: 1.019 ± 0.041
0.698TrpThr: 0.698 ± 0.031
1.135TrpVal: 1.135 ± 0.045
0.334TrpTrp: 0.334 ± 0.024
0.277TrpTyr: 0.277 ± 0.018
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.312TyrAla: 2.312 ± 0.065
0.138TyrCys: 0.138 ± 0.014
1.245TyrAsp: 1.245 ± 0.05
1.148TyrGlu: 1.148 ± 0.041
0.713TyrPhe: 0.713 ± 0.034
1.849TyrGly: 1.849 ± 0.048
0.27TyrHis: 0.27 ± 0.02
0.685TyrIle: 0.685 ± 0.031
0.287TyrLys: 0.287 ± 0.025
2.177TyrLeu: 2.177 ± 0.05
0.208TyrMet: 0.208 ± 0.02
0.425TyrAsn: 0.425 ± 0.033
0.954TyrPro: 0.954 ± 0.039
0.521TyrGln: 0.521 ± 0.027
1.672TyrArg: 1.672 ± 0.06
1.163TyrSer: 1.163 ± 0.054
1.142TyrThr: 1.142 ± 0.068
1.562TyrVal: 1.562 ± 0.051
0.277TyrTrp: 0.277 ± 0.018
0.419TyrTyr: 0.419 ± 0.027
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2131 proteins (688945 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski