Clostridium phage phiCDKH01

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.65

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 66 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6B9J3Z6|A0A6B9J3Z6_9CAUD Uncharacterized protein OS=Clostridium phage phiCDKH01 OX=2686203 GN=phiCDKH01_09 PE=4 SV=1
MM1 pKa = 7.69ADD3 pKa = 3.27MAVEE7 pKa = 4.28LLAMADD13 pKa = 3.51MAVEE17 pKa = 4.31LLAMADD23 pKa = 3.51MAVEE27 pKa = 4.31LLAMADD33 pKa = 3.51MAVEE37 pKa = 4.31LLAMADD43 pKa = 3.37MAVEE47 pKa = 3.95LEE49 pKa = 3.76ITIFKK54 pKa = 10.64NPEE57 pKa = 3.42LFIIVFTAICEE68 pKa = 3.98LATAFIAICEE78 pKa = 4.05LATAFIAICEE88 pKa = 4.05LATAFIAICEE98 pKa = 4.05LATAFIAICEE108 pKa = 4.05LATAFIAICEE118 pKa = 4.05LATAFIAICEE128 pKa = 4.05LATAFIAICEE138 pKa = 4.05LATAFIAICEE148 pKa = 4.05LATAFIAICEE158 pKa = 4.01LATAFMYY165 pKa = 10.91LSLLANASEE174 pKa = 4.11VLIFILSIPASFAAVFAIAKK194 pKa = 9.28SISLIEE200 pKa = 4.18LKK202 pKa = 9.95ATSSLDD208 pKa = 3.31KK209 pKa = 11.19AVITLKK215 pKa = 10.46LFSMHH220 pKa = 6.74

Molecular weight: 23.28 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6B9J074|A0A6B9J074_9CAUD Uncharacterized protein OS=Clostridium phage phiCDKH01 OX=2686203 GN=phiCDKH01_21 PE=4 SV=1
MM1 pKa = 7.51GKK3 pKa = 9.98LEE5 pKa = 3.84RR6 pKa = 11.84RR7 pKa = 11.84KK8 pKa = 10.35NKK10 pKa = 10.19RR11 pKa = 11.84EE12 pKa = 3.74NKK14 pKa = 9.32FNKK17 pKa = 9.32IKK19 pKa = 10.77NAFSFTLVLINLVLAILRR37 pKa = 11.84LIKK40 pKa = 10.35EE41 pKa = 4.16LL42 pKa = 3.81

Molecular weight: 5.01 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

66

0

66

12999

37

1842

197.0

22.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.154 ± 0.576

0.892 ± 0.153

5.916 ± 0.225

9.239 ± 0.627

3.593 ± 0.261

5.139 ± 0.299

1.031 ± 0.148

9.047 ± 0.377

10.385 ± 0.456

8.185 ± 0.38

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.431 ± 0.252

7.139 ± 0.467

2.069 ± 0.227

3.2 ± 0.277

3.546 ± 0.299

6.662 ± 0.387

6.024 ± 0.345

5.147 ± 0.256

0.977 ± 0.132

4.223 ± 0.386
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski