Salipiger thiooxidans

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Roseobacteraceae; Salipiger

Average proteome isoelectric point is 6.16

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5563 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1G7H6U2|A0A1G7H6U2_9RHOB Sec translocon accessory complex subunit YajC OS=Salipiger thiooxidans OX=282683 GN=yajC PE=3 SV=1
MM1 pKa = 8.22DD2 pKa = 5.84DD3 pKa = 4.5APLEE7 pKa = 4.39GAPLIAPSSTDD18 pKa = 3.2HH19 pKa = 7.06PLHH22 pKa = 6.31EE23 pKa = 4.89PVVEE27 pKa = 4.38ACRR30 pKa = 11.84SVYY33 pKa = 10.64DD34 pKa = 3.59PEE36 pKa = 5.2IPVNIYY42 pKa = 10.8DD43 pKa = 4.42LGLIYY48 pKa = 10.22TIAIDD53 pKa = 3.55PEE55 pKa = 4.2NAVKK59 pKa = 9.45VTMTLTAPGCPVAGEE74 pKa = 4.13MPGWVQDD81 pKa = 3.68AVSAVPGVRR90 pKa = 11.84DD91 pKa = 3.28VDD93 pKa = 3.22VDD95 pKa = 4.0MVFDD99 pKa = 4.94PPWGMDD105 pKa = 3.22MMSDD109 pKa = 3.68EE110 pKa = 4.89ARR112 pKa = 11.84LEE114 pKa = 4.14LGFMM118 pKa = 4.54

Molecular weight: 12.7 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1G7M1Y3|A0A1G7M1Y3_9RHOB Transport permease protein OS=Salipiger thiooxidans OX=282683 GN=SAMN04488105_1294 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84KK13 pKa = 9.18RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.36GGRR28 pKa = 11.84AVLNARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.03RR41 pKa = 11.84LSAA44 pKa = 4.03

Molecular weight: 5.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5563

0

5563

1713591

39

2874

308.0

33.35

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.642 ± 0.042

0.933 ± 0.01

5.837 ± 0.031

6.127 ± 0.03

3.628 ± 0.021

8.947 ± 0.032

2.038 ± 0.017

4.862 ± 0.025

2.669 ± 0.027

10.33 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.75 ± 0.017

2.315 ± 0.018

5.328 ± 0.028

2.973 ± 0.017

7.16 ± 0.038

5.144 ± 0.023

5.409 ± 0.025

7.34 ± 0.026

1.416 ± 0.013

2.154 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski