Psychrosphaera haliotis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales; Pseudoalteromonadaceae; Psychrosphaera

Average proteome isoelectric point is 6.14

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2692 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6N8F4U3|A0A6N8F4U3_9GAMM Pyridoxine/pyridoxamine 5'-phosphate oxidase OS=Psychrosphaera haliotis OX=555083 GN=pdxH PE=3 SV=1
MM1 pKa = 7.42KK2 pKa = 10.36KK3 pKa = 10.19IITALGLALASANVSAAPLVDD24 pKa = 3.53VWAGAYY30 pKa = 8.95AWNTTYY36 pKa = 10.96EE37 pKa = 4.62GVVSANPSSLDD48 pKa = 3.66LQDD51 pKa = 5.72DD52 pKa = 4.55LNFDD56 pKa = 4.7DD57 pKa = 4.52NTNNVIWAAFEE68 pKa = 4.3HH69 pKa = 7.13PIPLIPNIQIKK80 pKa = 7.64QTSLDD85 pKa = 3.62TTGFAQLDD93 pKa = 3.53NNYY96 pKa = 9.82NFGGDD101 pKa = 3.26TTGNGRR107 pKa = 11.84QDD109 pKa = 3.03TAADD113 pKa = 4.01LTHH116 pKa = 7.05TDD118 pKa = 3.3YY119 pKa = 11.14TLYY122 pKa = 10.11WGLPLPIATVDD133 pKa = 3.82FGLNVRR139 pKa = 11.84KK140 pKa = 9.58FDD142 pKa = 3.8GFVQIGDD149 pKa = 4.86AIAEE153 pKa = 4.1LDD155 pKa = 3.96APVPMLFARR164 pKa = 11.84VGAEE168 pKa = 4.07LPFTGLALMGEE179 pKa = 4.52ANYY182 pKa = 10.49VGYY185 pKa = 11.07GDD187 pKa = 4.4TNHH190 pKa = 6.83TDD192 pKa = 3.31YY193 pKa = 11.35QIVLRR198 pKa = 11.84YY199 pKa = 6.71TLPMIPLLDD208 pKa = 3.81VNLEE212 pKa = 3.62AGYY215 pKa = 10.76RR216 pKa = 11.84SFQLDD221 pKa = 3.31IDD223 pKa = 4.01PTDD226 pKa = 3.68FDD228 pKa = 4.79GDD230 pKa = 4.08EE231 pKa = 4.97DD232 pKa = 6.04DD233 pKa = 5.99LMADD237 pKa = 3.47IDD239 pKa = 3.79MSGAFVGVSLHH250 pKa = 6.05FF251 pKa = 4.29

Molecular weight: 27.27 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6N8FBS3|A0A6N8FBS3_9GAMM Uncharacterized protein OS=Psychrosphaera haliotis OX=555083 GN=GNP35_08110 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.49RR12 pKa = 11.84KK13 pKa = 7.97RR14 pKa = 11.84THH16 pKa = 5.89GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.3NGRR28 pKa = 11.84AVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.96GRR39 pKa = 11.84KK40 pKa = 8.63VLSKK44 pKa = 11.07

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2692

0

2692

866941

35

3376

322.0

35.82

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.959 ± 0.049

0.828 ± 0.014

5.772 ± 0.065

6.488 ± 0.052

4.423 ± 0.035

6.413 ± 0.055

1.974 ± 0.025

6.732 ± 0.039

6.174 ± 0.061

10.062 ± 0.059

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.39 ± 0.028

5.135 ± 0.04

3.601 ± 0.025

4.232 ± 0.037

3.845 ± 0.035

7.239 ± 0.047

5.503 ± 0.055

7.063 ± 0.041

1.107 ± 0.017

3.06 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski