Trichobilharzia regenti (Nasal bird schistosome)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Spiralia; Lophotrochozoa; Platyhelminthes; Trematoda; Digenea; Strigeidida; Schistosomatoidea; Schistosomatidae; Trichobilharzia

Average proteome isoelectric point is 6.7

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 22165 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A3P8JUP6|A0A3P8JUP6_TRIRE Uncharacterized protein OS=Trichobilharzia regenti OX=157069 GN=TRE_LOCUS18309 PE=4 SV=1
MM1 pKa = 8.06DD2 pKa = 4.63EE3 pKa = 4.42SSSVSGTASVLGEE16 pKa = 4.14TQLTSTLLYY25 pKa = 10.66DD26 pKa = 4.15VNPLTNSNNDD36 pKa = 3.16NNNNDD41 pKa = 3.89NNHH44 pKa = 6.23HH45 pKa = 6.69FSLTCSDD52 pKa = 3.68STEE55 pKa = 4.23SPLLNSTNIITTSSDD70 pKa = 3.26QQQSLSVMCTVNQNDD85 pKa = 3.73LSNNSGDD92 pKa = 3.56GGGGGEE98 pKa = 3.85QSIFDD103 pKa = 4.11TVTVLNSTPSAIHH116 pKa = 6.35SEE118 pKa = 4.23VLQEE122 pKa = 4.08STISLEE128 pKa = 4.16LTTSDD133 pKa = 4.32SNDD136 pKa = 2.9ASFSSLNSEE145 pKa = 5.62RR146 pKa = 11.84IDD148 pKa = 3.95CLKK151 pKa = 10.78EE152 pKa = 3.51YY153 pKa = 11.17LNFNKK158 pKa = 9.86TPATEE163 pKa = 4.18TLNNNSIVYY172 pKa = 7.93TLKK175 pKa = 11.02DD176 pKa = 3.43SLHH179 pKa = 6.63SNNNNNNDD187 pKa = 3.51NTNNTDD193 pKa = 3.79LNNSPLTNDD202 pKa = 3.58LSDD205 pKa = 3.8LTSHH209 pKa = 6.33VNNSSTSSATTTVALTSPICQLPTSVV235 pKa = 4.0

Molecular weight:
25.0 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A3P8INS7|A0A3P8INS7_TRIRE Uncharacterized protein OS=Trichobilharzia regenti OX=157069 GN=TRE_LOCUS2059 PE=4 SV=1
MM1 pKa = 7.14MSRR4 pKa = 11.84RR5 pKa = 11.84TLLKK9 pKa = 10.05VILTVRR15 pKa = 11.84PQIVPMLNLRR25 pKa = 11.84RR26 pKa = 11.84NRR28 pKa = 11.84NRR30 pKa = 11.84SS31 pKa = 3.1

Molecular weight:
3.76 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

22165

0

22165

4891284

29

3889

220.7

24.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.948 ± 0.017

2.138 ± 0.012

5.177 ± 0.019

5.434 ± 0.02

3.74 ± 0.014

4.529 ± 0.02

2.826 ± 0.011

5.897 ± 0.015

5.415 ± 0.022

9.249 ± 0.025

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.23 ± 0.008

6.685 ± 0.026

4.771 ± 0.019

4.298 ± 0.017

4.917 ± 0.016

10.507 ± 0.035

6.457 ± 0.026

5.531 ± 0.015

0.924 ± 0.005

3.199 ± 0.012

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski