Pectobacterium phage MA6

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Studiervirinae; Pektosvirus; unclassified Pektosvirus

Average proteome isoelectric point is 6.84

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 38 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5Q2F6U8|A0A5Q2F6U8_9CAUD N-acetylmuramoyl-L-alanine amidase OS=Pectobacterium phage MA6 OX=2608323 GN=MA6_gp27 PE=3 SV=1
MM1 pKa = 8.04AIQDD5 pKa = 4.18LNVDD9 pKa = 4.0SVAEE13 pKa = 3.94LDD15 pKa = 3.68AVNDD19 pKa = 3.33ILAAIGEE26 pKa = 4.51APVNTLMDD34 pKa = 4.69DD35 pKa = 3.87SNADD39 pKa = 3.1VANARR44 pKa = 11.84RR45 pKa = 11.84ILNTTNRR52 pKa = 11.84KK53 pKa = 7.17IQSRR57 pKa = 11.84GWTFNIEE64 pKa = 3.95EE65 pKa = 4.73GATLQPDD72 pKa = 3.97VFSNLIDD79 pKa = 3.58YY80 pKa = 11.22SNDD83 pKa = 3.1YY84 pKa = 10.56LSVMSTGSTSAYY96 pKa = 9.38VNRR99 pKa = 11.84GGYY102 pKa = 10.37LYY104 pKa = 10.76DD105 pKa = 3.41RR106 pKa = 11.84TALTDD111 pKa = 3.29QFTTAVSVNLIRR123 pKa = 11.84LRR125 pKa = 11.84AFDD128 pKa = 5.57DD129 pKa = 3.47MPEE132 pKa = 4.16CFRR135 pKa = 11.84TLIVTEE141 pKa = 4.15AARR144 pKa = 11.84MFNSRR149 pKa = 11.84YY150 pKa = 9.38FGAPEE155 pKa = 3.54IEE157 pKa = 3.84AVLRR161 pKa = 11.84SEE163 pKa = 3.99ILEE166 pKa = 4.17AKK168 pKa = 8.66AACMEE173 pKa = 3.97YY174 pKa = 10.86EE175 pKa = 3.95MDD177 pKa = 3.51YY178 pKa = 11.53GKK180 pKa = 11.19YY181 pKa = 10.96NMLDD185 pKa = 3.14GDD187 pKa = 3.96AHH189 pKa = 5.44VQGLLSRR196 pKa = 4.51

Molecular weight: 21.79 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5Q2EZM8|A0A5Q2EZM8_9CAUD S-adenosyl-L-methionine hydrolase OS=Pectobacterium phage MA6 OX=2608323 GN=MA6_gp41 PE=4 SV=1
MM1 pKa = 7.65SLRR4 pKa = 11.84KK5 pKa = 9.98VEE7 pKa = 3.71VRR9 pKa = 11.84VIVEE13 pKa = 3.91THH15 pKa = 4.48IHH17 pKa = 6.19GGTAKK22 pKa = 10.74AADD25 pKa = 3.75KK26 pKa = 10.92LGNVYY31 pKa = 9.48QWIRR35 pKa = 11.84QRR37 pKa = 11.84GVWEE41 pKa = 3.86YY42 pKa = 7.32WTNEE46 pKa = 3.11TDD48 pKa = 3.29AFRR51 pKa = 11.84VEE53 pKa = 4.04HH54 pKa = 6.27LTRR57 pKa = 11.84SWVNIPRR64 pKa = 11.84RR65 pKa = 11.84IFHH68 pKa = 5.32GTFYY72 pKa = 10.78IKK74 pKa = 10.47VAAA77 pKa = 4.41

Molecular weight: 9.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

38

0

38

11393

77

1340

299.8

33.5

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.777 ± 0.537

1.053 ± 0.218

6.144 ± 0.212

6.434 ± 0.266

3.844 ± 0.235

7.496 ± 0.398

1.966 ± 0.228

4.977 ± 0.3

6.53 ± 0.34

7.821 ± 0.333

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.861 ± 0.242

4.836 ± 0.212

3.643 ± 0.194

4.143 ± 0.312

5.442 ± 0.236

6.091 ± 0.371

5.679 ± 0.319

7.092 ± 0.311

1.554 ± 0.155

3.616 ± 0.223
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski