spirochete symbiont of Stewartia floridana

Taxonomy: cellular organisms; Bacteria; Spirochaetes; Spirochaetia; Spirochaetales; unclassified Spirochaetales

Average proteome isoelectric point is 6.57

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 817 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A421K8W2|A0A421K8W2_9SPIR Uncharacterized protein OS=spirochete symbiont of Stewartia floridana OX=1968597 GN=B6D68_03135 PE=4 SV=1
MM1 pKa = 7.66KK2 pKa = 10.15NYY4 pKa = 10.45QNITLSLLVFTLFWGCQASLPDD26 pKa = 3.6NLEE29 pKa = 4.01TLGEE33 pKa = 4.35SGLAWEE39 pKa = 4.78MSTLTSEE46 pKa = 5.38DD47 pKa = 3.81DD48 pKa = 4.39LNVLLARR55 pKa = 11.84SGEE58 pKa = 4.41GIVDD62 pKa = 3.45WQLSRR67 pKa = 11.84EE68 pKa = 4.14FAAMALEE75 pKa = 4.03EE76 pKa = 5.25FIEE79 pKa = 4.3EE80 pKa = 4.05DD81 pKa = 3.38NYY83 pKa = 10.98PPEE86 pKa = 5.16SEE88 pKa = 4.77LWDD91 pKa = 3.61IPIAIYY97 pKa = 10.56DD98 pKa = 3.77DD99 pKa = 3.91EE100 pKa = 6.46GEE102 pKa = 3.95VRR104 pKa = 11.84YY105 pKa = 10.27YY106 pKa = 10.42EE107 pKa = 3.95FRR109 pKa = 11.84IVDD112 pKa = 3.92GEE114 pKa = 4.24QTLGAIAGNARR125 pKa = 11.84EE126 pKa = 4.33DD127 pKa = 3.13RR128 pKa = 11.84GAPVSHH134 pKa = 6.2VFEE137 pKa = 4.18MDD139 pKa = 3.57GYY141 pKa = 11.19ADD143 pKa = 4.12EE144 pKa = 5.72LSALYY149 pKa = 10.58ASGAMSADD157 pKa = 4.43DD158 pKa = 3.72IPRR161 pKa = 11.84IVDD164 pKa = 3.24NDD166 pKa = 3.71YY167 pKa = 10.79PSYY170 pKa = 11.26AVGSVTLTRR179 pKa = 11.84SGNVDD184 pKa = 3.3LKK186 pKa = 11.11EE187 pKa = 4.06IVDD190 pKa = 4.0PSTGEE195 pKa = 3.65QLEE198 pKa = 4.29NLVVLLDD205 pKa = 3.83MEE207 pKa = 4.49EE208 pKa = 4.66SIAAYY213 pKa = 9.57PDD215 pKa = 3.41TT216 pKa = 4.74

Molecular weight: 23.91 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A421K9E7|A0A421K9E7_9SPIR Uncharacterized protein OS=spirochete symbiont of Stewartia floridana OX=1968597 GN=B6D68_02115 PE=4 SV=1
MM1 pKa = 7.08SQTRR5 pKa = 11.84SLAGLLISIVFMAILCSPQALHH27 pKa = 7.01PVLQTLFPQAGEE39 pKa = 4.13WVFPRR44 pKa = 11.84ATLIRR49 pKa = 11.84LTIQHH54 pKa = 6.48LTLSLTAAGIAALIGILLAITATRR78 pKa = 11.84RR79 pKa = 11.84HH80 pKa = 5.12GRR82 pKa = 11.84AFLRR86 pKa = 11.84MIRR89 pKa = 11.84DD90 pKa = 3.69VSSLAQTIPPTAVLALAIPFLGFGYY115 pKa = 10.4KK116 pKa = 9.64PVLLALVLYY125 pKa = 10.67SILPVLNNTLTALEE139 pKa = 4.81CLPKK143 pKa = 10.31PILEE147 pKa = 4.56AAQAMGMKK155 pKa = 9.88SLQVLFLIEE164 pKa = 4.8LPLALPAIGTGIRR177 pKa = 11.84ISAVINIGTATIAALAGAGGLGSPIVAGLVRR208 pKa = 11.84DD209 pKa = 3.76NPAWVFQGAASSALLALILNAILLRR234 pKa = 11.84VEE236 pKa = 4.78VKK238 pKa = 10.79LNPAKK243 pKa = 10.71

Molecular weight: 25.45 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

817

0

817

230933

27

1508

282.7

31.24

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.175 ± 0.093

1.121 ± 0.037

5.492 ± 0.065

6.437 ± 0.068

3.978 ± 0.06

8.227 ± 0.068

2.131 ± 0.039

6.909 ± 0.067

4.527 ± 0.067

10.049 ± 0.097

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.724 ± 0.041

3.432 ± 0.046

4.64 ± 0.058

2.901 ± 0.046

6.537 ± 0.075

6.479 ± 0.055

4.694 ± 0.054

6.462 ± 0.071

1.287 ± 0.034

2.796 ± 0.051
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski