Synechococcus sp. RSCCF101

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Cyanobacteria/Melainabacteria group; Cyanobacteria; Synechococcales; Synechococcaceae; Synechococcus; unclassified Synechococcus

Average proteome isoelectric point is 6.67

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2660 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5J6Q531|A0A5J6Q531_9SYNE Peptidyl-tRNA hydrolase OS=Synechococcus sp. RSCCF101 OX=2511069 GN=pth PE=3 SV=1
MM1 pKa = 6.81QWEE4 pKa = 4.41FQEE7 pKa = 4.8DD8 pKa = 3.69AAFLALCDD16 pKa = 3.64AFKK19 pKa = 11.03EE20 pKa = 4.38SGEE23 pKa = 4.06SSAIEE28 pKa = 3.79FLAHH32 pKa = 6.67GEE34 pKa = 4.38GAFHH38 pKa = 6.71FQEE41 pKa = 4.32LTQNAAGEE49 pKa = 4.49GVDD52 pKa = 5.68LSDD55 pKa = 5.64SDD57 pKa = 5.03SLDD60 pKa = 4.09DD61 pKa = 4.2FQQEE65 pKa = 4.46VIDD68 pKa = 4.26TMEE71 pKa = 4.54ALCSS75 pKa = 3.6

Molecular weight: 8.22 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5J6PY12|A0A5J6PY12_9SYNE Site-specific integrase OS=Synechococcus sp. RSCCF101 OX=2511069 GN=EVJ50_02990 PE=4 SV=1
MM1 pKa = 7.51GSDD4 pKa = 3.24HH5 pKa = 6.97HH6 pKa = 8.08RR7 pKa = 11.84GEE9 pKa = 4.37PSVGTEE15 pKa = 3.54TWRR18 pKa = 11.84QRR20 pKa = 11.84TLLRR24 pKa = 11.84DD25 pKa = 4.04FDD27 pKa = 3.73RR28 pKa = 11.84TAWLLRR34 pKa = 11.84ADD36 pKa = 4.12LVQRR40 pKa = 11.84YY41 pKa = 7.78GEE43 pKa = 4.02RR44 pKa = 11.84DD45 pKa = 3.21AEE47 pKa = 3.92RR48 pKa = 11.84LARR51 pKa = 11.84HH52 pKa = 5.2ARR54 pKa = 11.84VRR56 pKa = 11.84CMEE59 pKa = 3.98ILGSVSWVKK68 pKa = 10.73GRR70 pKa = 11.84GAAVMNGFLWISAQEE85 pKa = 3.89LALFQAIEE93 pKa = 3.98ARR95 pKa = 11.84GGDD98 pKa = 3.78AAEE101 pKa = 4.25AWAICHH107 pKa = 5.7KK108 pKa = 10.48AIRR111 pKa = 11.84LRR113 pKa = 11.84VEE115 pKa = 5.62RR116 pKa = 11.84IPGWKK121 pKa = 8.48RR122 pKa = 11.84WLMRR126 pKa = 11.84RR127 pKa = 11.84LLFSALVRR135 pKa = 11.84RR136 pKa = 11.84IIRR139 pKa = 11.84RR140 pKa = 11.84RR141 pKa = 11.84AVRR144 pKa = 11.84GDD146 pKa = 3.11IVRR149 pKa = 11.84AGDD152 pKa = 3.58FKK154 pKa = 10.81TRR156 pKa = 11.84SLIGDD161 pKa = 4.63GIAFDD166 pKa = 5.02LGVDD170 pKa = 3.56YY171 pKa = 10.79LRR173 pKa = 11.84CGNLEE178 pKa = 3.94LARR181 pKa = 11.84TIGAEE186 pKa = 3.77TFAPYY191 pKa = 10.74LCLSDD196 pKa = 3.6IALSEE201 pKa = 4.01GLGWGLIRR209 pKa = 11.84TQTLADD215 pKa = 4.01GCSHH219 pKa = 7.09CDD221 pKa = 3.51FRR223 pKa = 11.84FKK225 pKa = 10.81QGGPTRR231 pKa = 11.84IKK233 pKa = 10.54SRR235 pKa = 11.84TPEE238 pKa = 3.76VQATLEE244 pKa = 4.35RR245 pKa = 11.84IARR248 pKa = 11.84TEE250 pKa = 4.01AEE252 pKa = 4.25LAATAQSLRR261 pKa = 3.77

Molecular weight: 29.43 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2660

0

2660

856344

30

11376

321.9

34.87

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.741 ± 0.063

1.163 ± 0.025

5.452 ± 0.115

5.849 ± 0.052

2.851 ± 0.039

8.963 ± 0.065

2.077 ± 0.033

3.779 ± 0.041

1.613 ± 0.029

12.578 ± 0.094

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.831 ± 0.033

2.06 ± 0.042

6.208 ± 0.056

4.003 ± 0.04

8.457 ± 0.115

6.377 ± 0.075

4.761 ± 0.101

6.894 ± 0.05

1.695 ± 0.031

1.649 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski