Edwardsiella phage PEi21

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Yokohamavirus; Edwardsiella virus PEi21

Average proteome isoelectric point is 6.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 71 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|N0DP77|N0DP77_9CAUD Uncharacterized protein OS=Edwardsiella phage PEi21 OX=1325372 PE=4 SV=1
MM1 pKa = 7.1ITIIDD6 pKa = 3.81EE7 pKa = 4.34NNIVTEE13 pKa = 4.06NGVEE17 pKa = 4.02LVAEE21 pKa = 4.97EE22 pKa = 5.26GYY24 pKa = 10.14LWCGEE29 pKa = 4.72CYY31 pKa = 10.44FSPLNADD38 pKa = 3.55GCSLTDD44 pKa = 3.55GLCIRR49 pKa = 11.84AYY51 pKa = 10.59RR52 pKa = 11.84DD53 pKa = 3.24DD54 pKa = 4.24DD55 pKa = 4.47RR56 pKa = 11.84DD57 pKa = 3.79VIFVEE62 pKa = 5.18KK63 pKa = 10.74NPCC66 pKa = 3.36

Molecular weight: 7.42 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|N0DQP3|N0DQP3_9CAUD Uncharacterized protein OS=Edwardsiella phage PEi21 OX=1325372 PE=4 SV=1
MM1 pKa = 7.53SARR4 pKa = 11.84TPTYY8 pKa = 10.38NGTVCLRR15 pKa = 11.84GHH17 pKa = 5.27TEE19 pKa = 3.98RR20 pKa = 11.84YY21 pKa = 8.68VATGKK26 pKa = 10.44CVEE29 pKa = 4.39CDD31 pKa = 3.14RR32 pKa = 11.84MRR34 pKa = 11.84KK35 pKa = 7.91RR36 pKa = 11.84ASYY39 pKa = 10.59ARR41 pKa = 11.84NAKK44 pKa = 9.47NFNHH48 pKa = 6.56PVMVFGNPNRR58 pKa = 11.84TGVV61 pKa = 3.16

Molecular weight: 6.89 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

71

0

71

13609

37

782

191.7

21.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.589 ± 0.375

1.565 ± 0.15

6.371 ± 0.217

5.416 ± 0.262

3.358 ± 0.182

7.855 ± 0.295

1.903 ± 0.186

5.254 ± 0.203

5.026 ± 0.218

7.289 ± 0.303

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.947 ± 0.157

4.534 ± 0.234

4.203 ± 0.31

3.365 ± 0.203

6.018 ± 0.282

5.959 ± 0.218

6.716 ± 0.331

7.524 ± 0.296

1.639 ± 0.105

3.468 ± 0.155
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski