Methylobacterium sp. TER-1

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Methylobacteriaceae; Methylobacterium; unclassified Methylobacterium

Average proteome isoelectric point is 6.93

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5625 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3S2VK25|A0A3S2VK25_9RHIZ EAL domain-containing protein OS=Methylobacterium sp. TER-1 OX=2499852 GN=EOE48_22345 PE=4 SV=1
MM1 pKa = 7.5ATTSYY6 pKa = 11.18YY7 pKa = 10.49FGYY10 pKa = 10.64LNRR13 pKa = 11.84NEE15 pKa = 4.37PLNTYY20 pKa = 9.27TYY22 pKa = 10.44LSQYY26 pKa = 10.83ADD28 pKa = 3.9TITALNNGGFFTAYY42 pKa = 9.47EE43 pKa = 4.57GYY45 pKa = 10.82YY46 pKa = 10.6DD47 pKa = 4.1GGYY50 pKa = 10.79NYY52 pKa = 10.19ILARR56 pKa = 11.84GLDD59 pKa = 3.75ATGAPAPKK67 pKa = 10.64GEE69 pKa = 4.26FAATNYY75 pKa = 10.49GPGINVRR82 pKa = 11.84SAEE85 pKa = 3.95AATLKK90 pKa = 10.7NGNVVMTYY98 pKa = 7.04TTQSSTSTPITFRR111 pKa = 11.84IFSPDD116 pKa = 2.91GTLLTEE122 pKa = 4.96GSTASGSNYY131 pKa = 10.18SPDD134 pKa = 3.16VTALGDD140 pKa = 3.5GGFVISYY147 pKa = 10.05DD148 pKa = 3.64HH149 pKa = 7.03LFGSTDD155 pKa = 4.01LDD157 pKa = 3.69TQAVVFNADD166 pKa = 3.11GTVRR170 pKa = 11.84TSLAVNVGTYY180 pKa = 7.26YY181 pKa = 10.43TGQSAVAGLTNGNFMVSYY199 pKa = 10.48ARR201 pKa = 11.84EE202 pKa = 3.96RR203 pKa = 11.84QDD205 pKa = 3.85GSGATDD211 pKa = 3.63LAFKK215 pKa = 10.49IFNANGGVVRR225 pKa = 11.84AEE227 pKa = 4.26TLADD231 pKa = 3.34TYY233 pKa = 11.35GTMKK237 pKa = 10.53VPGEE241 pKa = 4.28AIGLKK246 pKa = 10.5DD247 pKa = 3.28GGFAIVYY254 pKa = 10.0FDD256 pKa = 5.27DD257 pKa = 3.8GWTGRR262 pKa = 11.84PAEE265 pKa = 4.2VTLGIWNADD274 pKa = 2.87GSLRR278 pKa = 11.84TIQRR282 pKa = 11.84ASEE285 pKa = 3.81PLTRR289 pKa = 11.84GGQRR293 pKa = 11.84NDD295 pKa = 3.2PTIAQLDD302 pKa = 3.8NGFLAVGWSNVTDD315 pKa = 3.48VTTEE319 pKa = 3.9YY320 pKa = 10.94AIWTPDD326 pKa = 2.92GTAVTSGLWRR336 pKa = 11.84GTSQNLNFAAAANGVLSGIVTDD358 pKa = 4.67TIGDD362 pKa = 3.63GSGYY366 pKa = 10.6GIASNTLAFTRR377 pKa = 11.84DD378 pKa = 3.22TRR380 pKa = 11.84GDD382 pKa = 3.49ASSEE386 pKa = 4.2AITGDD391 pKa = 3.56SLRR394 pKa = 11.84DD395 pKa = 3.57FMMGGGGNDD404 pKa = 3.16TLLGYY409 pKa = 10.39GADD412 pKa = 3.78DD413 pKa = 4.01TLDD416 pKa = 3.63GGIGNDD422 pKa = 3.86SVDD425 pKa = 3.38GGIGNDD431 pKa = 3.84LVSGGAGADD440 pKa = 3.26ILIGGTGNDD449 pKa = 3.65TLDD452 pKa = 3.84GGSEE456 pKa = 3.96NDD458 pKa = 3.87RR459 pKa = 11.84LDD461 pKa = 3.86GGSGDD466 pKa = 4.56DD467 pKa = 4.66LLTGGLGADD476 pKa = 3.57ALLGRR481 pKa = 11.84DD482 pKa = 3.77GFDD485 pKa = 2.99TASYY489 pKa = 9.95AGAVASLTADD499 pKa = 4.06LLTPANNTGEE509 pKa = 4.26AAGDD513 pKa = 3.71SYY515 pKa = 12.04AGIEE519 pKa = 4.11GLLGSRR525 pKa = 11.84YY526 pKa = 9.45SDD528 pKa = 3.06RR529 pKa = 11.84LTGNATANRR538 pKa = 11.84LEE540 pKa = 4.51GGAGNDD546 pKa = 3.93TLDD549 pKa = 3.94GGAGNDD555 pKa = 3.76TLVGGDD561 pKa = 3.6GADD564 pKa = 3.03WLYY567 pKa = 11.71ASAGYY572 pKa = 10.48DD573 pKa = 3.79LLTGGAGGDD582 pKa = 2.98RR583 pKa = 11.84FAFTSVSPQYY593 pKa = 10.21DD594 pKa = 3.66TIGDD598 pKa = 3.88FVHH601 pKa = 6.73AQGDD605 pKa = 4.34RR606 pKa = 11.84IVLTSSAFGGLTGLVAGKK624 pKa = 10.37SFVASGSPAAAQAGPTMLYY643 pKa = 8.68NTGNGVLSYY652 pKa = 10.98DD653 pKa = 4.09ADD655 pKa = 3.87GTGAGAATQIATLSGAPTLVFWDD678 pKa = 4.87FVFVV682 pKa = 3.6

Molecular weight: 69.37 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3S2V3S6|A0A3S2V3S6_9RHIZ ABC transporter ATP-binding protein OS=Methylobacterium sp. TER-1 OX=2499852 GN=EOE48_21900 PE=3 SV=1
RR1 pKa = 7.52RR2 pKa = 11.84RR3 pKa = 11.84PHH5 pKa = 6.35PGRR8 pKa = 11.84RR9 pKa = 11.84PAHH12 pKa = 6.09PRR14 pKa = 11.84PPAHH18 pKa = 6.47RR19 pKa = 11.84PHH21 pKa = 6.84RR22 pKa = 11.84RR23 pKa = 11.84RR24 pKa = 11.84PRR26 pKa = 11.84VVFPHH31 pKa = 6.06RR32 pKa = 11.84RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84HH36 pKa = 4.58RR37 pKa = 11.84PARR40 pKa = 11.84RR41 pKa = 11.84RR42 pKa = 11.84VRR44 pKa = 11.84PRR46 pKa = 11.84RR47 pKa = 11.84ARR49 pKa = 11.84RR50 pKa = 11.84PRR52 pKa = 11.84RR53 pKa = 11.84APARR57 pKa = 11.84PGLAPLSRR65 pKa = 11.84QGVRR69 pKa = 11.84PEE71 pKa = 3.92FQKK74 pKa = 11.32LEE76 pKa = 3.85AALAVTQPP84 pKa = 3.42

Molecular weight: 10.1 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5625

0

5625

1810136

29

8416

321.8

34.37

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.717 ± 0.061

0.776 ± 0.011

5.679 ± 0.03

5.28 ± 0.037

3.302 ± 0.019

9.591 ± 0.053

1.912 ± 0.019

4.042 ± 0.024

2.093 ± 0.027

10.62 ± 0.034

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.931 ± 0.017

1.971 ± 0.021

6.005 ± 0.031

2.602 ± 0.017

8.485 ± 0.046

4.64 ± 0.029

5.282 ± 0.048

7.851 ± 0.024

1.225 ± 0.013

1.997 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski