Escherichia phage phi G17

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Schitoviridae; Enquatrovirinae; Gamaleyavirus; unclassified Gamaleyavirus

Average proteome isoelectric point is 6.27

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 78 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2Z4PZX7|A0A2Z4PZX7_9CAUD Uncharacterized protein OS=Escherichia phage phi G17 OX=2234086 PE=4 SV=1
MM1 pKa = 6.64PTVYY5 pKa = 10.34VSAQQSTKK13 pKa = 10.07KK14 pKa = 10.13INYY17 pKa = 6.42STSPSMEE24 pKa = 4.66DD25 pKa = 3.08GFVAIGSFEE34 pKa = 4.71HH35 pKa = 7.56PDD37 pKa = 2.93ATYY40 pKa = 10.4PDD42 pKa = 4.99SYY44 pKa = 11.05VIWHH48 pKa = 7.52GIRR51 pKa = 11.84KK52 pKa = 8.63IAYY55 pKa = 7.59FRR57 pKa = 11.84NGGNVAQTAFFPDD70 pKa = 3.51NLTNLNDD77 pKa = 3.35WDD79 pKa = 3.73IYY81 pKa = 10.51EE82 pKa = 4.49VVPGSDD88 pKa = 3.55PVPVGFDD95 pKa = 2.97VYY97 pKa = 10.66RR98 pKa = 11.84YY99 pKa = 10.22DD100 pKa = 4.21PEE102 pKa = 4.11TEE104 pKa = 4.25EE105 pKa = 5.05ISRR108 pKa = 11.84LYY110 pKa = 10.55QDD112 pKa = 5.09GEE114 pKa = 4.26TLDD117 pKa = 3.61VYY119 pKa = 11.13EE120 pKa = 4.29GVRR123 pKa = 11.84YY124 pKa = 9.84LRR126 pKa = 11.84VVPKK130 pKa = 8.9PTGSTGTITLAVEE143 pKa = 4.49GEE145 pKa = 3.97AAASYY150 pKa = 9.97DD151 pKa = 3.33IKK153 pKa = 11.21LEE155 pKa = 3.86PTLGYY160 pKa = 10.86GVISYY165 pKa = 9.83KK166 pKa = 10.19WGTEE170 pKa = 3.88PSPGVYY176 pKa = 9.91DD177 pKa = 4.37AEE179 pKa = 4.18LTITKK184 pKa = 10.22DD185 pKa = 3.6GVSTTLQLAYY195 pKa = 10.5SALL198 pKa = 3.77

Molecular weight: 21.82 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2Z4PZY8|A0A2Z4PZY8_9CAUD Uncharacterized protein OS=Escherichia phage phi G17 OX=2234086 PE=4 SV=1
MM1 pKa = 7.08NLKK4 pKa = 10.4LFMMFEE10 pKa = 4.81GGVSTQEE17 pKa = 3.49IFNPRR22 pKa = 11.84WGLMSRR28 pKa = 11.84NKK30 pKa = 7.89VTVYY34 pKa = 9.57FRR36 pKa = 11.84PVRR39 pKa = 3.72

Molecular weight: 4.68 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

78

0

78

20545

37

3450

263.4

29.37

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.542 ± 0.682

0.862 ± 0.17

5.807 ± 0.163

6.663 ± 0.257

3.66 ± 0.197

6.659 ± 0.241

1.665 ± 0.156

5.505 ± 0.265

6.323 ± 0.257

8.304 ± 0.221

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.11 ± 0.148

5.578 ± 0.191

4.079 ± 0.172

4.347 ± 0.307

4.332 ± 0.169

6.225 ± 0.193

6.483 ± 0.289

6.8 ± 0.258

1.197 ± 0.117

3.86 ± 0.313
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski