Capybara microvirus Cap1_SP_223

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 5.6

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A4P8W5P8|A0A4P8W5P8_9VIRU Minor capsid protein OS=Capybara microvirus Cap1_SP_223 OX=2585414 PE=4 SV=1
MM1 pKa = 7.59ASIFRR6 pKa = 11.84TGPIVGQTFRR16 pKa = 11.84RR17 pKa = 11.84FYY19 pKa = 10.53PKK21 pKa = 10.58YY22 pKa = 10.14GLGKK26 pKa = 10.17DD27 pKa = 3.73GYY29 pKa = 10.46LVEE32 pKa = 4.61LPEE35 pKa = 5.39VIDD38 pKa = 3.65NQQIIDD44 pKa = 3.93DD45 pKa = 4.97AYY47 pKa = 10.99DD48 pKa = 3.97DD49 pKa = 4.24SLNVLLDD56 pKa = 3.44KK57 pKa = 11.12YY58 pKa = 10.45IYY60 pKa = 10.56EE61 pKa = 4.68PVLEE65 pKa = 4.59DD66 pKa = 3.69QNDD69 pKa = 3.52YY70 pKa = 11.47SDD72 pKa = 5.8DD73 pKa = 4.3YY74 pKa = 11.45IDD76 pKa = 3.73YY77 pKa = 10.84RR78 pKa = 11.84RR79 pKa = 11.84DD80 pKa = 3.3ANALEE85 pKa = 4.56SFLKK89 pKa = 10.5ADD91 pKa = 3.69EE92 pKa = 4.55LAAEE96 pKa = 4.26LRR98 pKa = 11.84VAYY101 pKa = 9.55GMPRR105 pKa = 11.84ASISDD110 pKa = 3.54LRR112 pKa = 11.84KK113 pKa = 10.41EE114 pKa = 3.57MDD116 pKa = 3.2KK117 pKa = 10.99RR118 pKa = 11.84FSKK121 pKa = 11.16YY122 pKa = 8.18EE123 pKa = 4.14SKK125 pKa = 10.84EE126 pKa = 3.69VTFDD130 pKa = 3.21EE131 pKa = 4.77KK132 pKa = 11.29AQNEE136 pKa = 4.35TVEE139 pKa = 4.19VGKK142 pKa = 10.53QEE144 pKa = 4.24VVV146 pKa = 2.95

Molecular weight:
16.93 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A4P8W874|A0A4P8W874_9VIRU Replication initiator protein OS=Capybara microvirus Cap1_SP_223 OX=2585414 PE=4 SV=1
MM1 pKa = 7.49KK2 pKa = 10.11CLSPFFTSLGPVPCGRR18 pKa = 11.84CINCQINRR26 pKa = 11.84SQQWSIRR33 pKa = 11.84CLDD36 pKa = 3.46EE37 pKa = 5.19FKK39 pKa = 10.12ITGIGFFLTLTFKK52 pKa = 10.27KK53 pKa = 9.19TDD55 pKa = 2.94GKK57 pKa = 10.7LRR59 pKa = 11.84RR60 pKa = 11.84KK61 pKa = 9.29PLQDD65 pKa = 3.06FLKK68 pKa = 10.35RR69 pKa = 11.84LRR71 pKa = 11.84KK72 pKa = 9.65EE73 pKa = 3.45ISPVKK78 pKa = 9.93IRR80 pKa = 11.84YY81 pKa = 7.7FGCGEE86 pKa = 4.06YY87 pKa = 10.56GSKK90 pKa = 10.44GLRR93 pKa = 11.84PHH95 pKa = 5.7YY96 pKa = 10.11HH97 pKa = 6.37LLVFGWRR104 pKa = 11.84PDD106 pKa = 3.52DD107 pKa = 3.71LVSFRR112 pKa = 11.84VDD114 pKa = 2.73RR115 pKa = 11.84KK116 pKa = 10.11GQILYY121 pKa = 10.26RR122 pKa = 11.84SALIEE127 pKa = 4.73RR128 pKa = 11.84IWHH131 pKa = 5.0SHH133 pKa = 4.34TDD135 pKa = 3.16NSYY138 pKa = 8.62VAGFISIGDD147 pKa = 3.72INEE150 pKa = 3.99VTCRR154 pKa = 11.84YY155 pKa = 9.36ASKK158 pKa = 10.44YY159 pKa = 9.49CSKK162 pKa = 10.72LGSFDD167 pKa = 3.8SEE169 pKa = 4.45YY170 pKa = 10.54PPFSAMSTHH179 pKa = 6.74PGIGASSVSLARR191 pKa = 11.84CWQGTIVYY199 pKa = 9.06QNKK202 pKa = 8.78VVPAPRR208 pKa = 11.84YY209 pKa = 10.17YY210 pKa = 9.09ITCLSRR216 pKa = 11.84QGVSVDD222 pKa = 4.22GILADD227 pKa = 3.83RR228 pKa = 11.84RR229 pKa = 11.84SRR231 pKa = 11.84SCLLNGDD238 pKa = 4.28YY239 pKa = 10.64PVLDD243 pKa = 3.63YY244 pKa = 11.9NEE246 pKa = 4.17IEE248 pKa = 4.34RR249 pKa = 11.84ACFEE253 pKa = 3.63ARR255 pKa = 11.84QRR257 pKa = 11.84LEE259 pKa = 3.8RR260 pKa = 11.84KK261 pKa = 9.01IPKK264 pKa = 10.05NGG266 pKa = 3.18

Molecular weight:
30.45 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

1292

75

527

258.4

28.9

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.43 ± 2.419

1.935 ± 0.878

6.192 ± 0.909

4.876 ± 0.983

5.031 ± 0.47

6.579 ± 0.852

1.625 ± 0.434

5.263 ± 0.694

3.87 ± 0.793

6.966 ± 0.744

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.858 ± 0.485

5.341 ± 1.018

4.412 ± 0.914

4.954 ± 0.654

5.805 ± 0.946

10.372 ± 1.322

4.954 ± 0.598

6.502 ± 0.751

1.471 ± 0.244

4.567 ± 0.515

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski