Tortoise microvirus 101

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 6.19

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4V1FW24|A0A4V1FW24_9VIRU Uncharacterized protein OS=Tortoise microvirus 101 OX=2583101 PE=4 SV=1
MM1 pKa = 7.41ARR3 pKa = 11.84VINNSNPDD11 pKa = 3.44KK12 pKa = 10.63VHH14 pKa = 5.61TPIRR18 pKa = 11.84QPFTVPNQTYY28 pKa = 8.39TIRR31 pKa = 11.84QLFEE35 pKa = 4.09RR36 pKa = 11.84MRR38 pKa = 11.84EE39 pKa = 4.14GLPMPQARR47 pKa = 11.84SQTYY51 pKa = 9.37YY52 pKa = 10.42EE53 pKa = 3.75SDD55 pKa = 3.9YY56 pKa = 11.4YY57 pKa = 11.03DD58 pKa = 3.66VEE60 pKa = 4.52SVEE63 pKa = 4.33PLSTPIDD70 pKa = 3.8LSDD73 pKa = 3.32ITDD76 pKa = 3.33MNFANLAAQRR86 pKa = 11.84AKK88 pKa = 10.72DD89 pKa = 3.82SEE91 pKa = 4.52SEE93 pKa = 4.12RR94 pKa = 11.84SSVADD99 pKa = 3.6EE100 pKa = 3.85PVEE103 pKa = 4.25ANLAVEE109 pKa = 4.97EE110 pKa = 4.25PPKK113 pKa = 10.64DD114 pKa = 3.44KK115 pKa = 11.07KK116 pKa = 10.8KK117 pKa = 11.02

Molecular weight: 13.36 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4P8W7R2|A0A4P8W7R2_9VIRU DNA pilot protein OS=Tortoise microvirus 101 OX=2583101 PE=4 SV=1
MM1 pKa = 6.8STPRR5 pKa = 11.84GSRR8 pKa = 11.84FITLTYY14 pKa = 11.04DD15 pKa = 3.75DD16 pKa = 5.01DD17 pKa = 4.45HH18 pKa = 7.88LVYY21 pKa = 10.38YY22 pKa = 10.18RR23 pKa = 11.84KK24 pKa = 9.72RR25 pKa = 11.84AANGEE30 pKa = 4.15WLSLQSDD37 pKa = 4.77GEE39 pKa = 4.21WQEE42 pKa = 3.86YY43 pKa = 8.23LCNYY47 pKa = 9.5VEE49 pKa = 5.0ALPGLKK55 pKa = 10.2KK56 pKa = 10.48SDD58 pKa = 2.94IQNYY62 pKa = 6.55MKK64 pKa = 10.8RR65 pKa = 11.84LRR67 pKa = 11.84KK68 pKa = 9.51KK69 pKa = 9.86LSVYY73 pKa = 9.22NRR75 pKa = 11.84KK76 pKa = 8.77IRR78 pKa = 11.84YY79 pKa = 8.31YY80 pKa = 10.61VVGEE84 pKa = 4.18YY85 pKa = 8.42GTKK88 pKa = 9.05TNRR91 pKa = 11.84PHH93 pKa = 5.17YY94 pKa = 8.87HH95 pKa = 6.74LIVFGVNQDD104 pKa = 3.84DD105 pKa = 4.05FNLFNSEE112 pKa = 4.13WYY114 pKa = 9.85HH115 pKa = 6.41GSIRR119 pKa = 11.84YY120 pKa = 9.7DD121 pKa = 3.41EE122 pKa = 4.43VTDD125 pKa = 4.24ASINYY130 pKa = 8.05ILKK133 pKa = 9.36YY134 pKa = 9.84HH135 pKa = 6.18VDD137 pKa = 3.81RR138 pKa = 11.84NEE140 pKa = 4.14HH141 pKa = 7.06LDD143 pKa = 3.55IQPVKK148 pKa = 10.4PFALMSKK155 pKa = 10.54GIGRR159 pKa = 11.84VYY161 pKa = 9.45IDD163 pKa = 3.71KK164 pKa = 10.18MKK166 pKa = 10.46SYY168 pKa = 10.31HH169 pKa = 6.52RR170 pKa = 11.84NSGDD174 pKa = 3.28MLVTVYY180 pKa = 10.7GSKK183 pKa = 10.59KK184 pKa = 10.25RR185 pKa = 11.84MPRR188 pKa = 11.84YY189 pKa = 8.3YY190 pKa = 10.31KK191 pKa = 10.48EE192 pKa = 4.33RR193 pKa = 11.84MFSALEE199 pKa = 3.83RR200 pKa = 11.84EE201 pKa = 4.44IQCRR205 pKa = 11.84EE206 pKa = 3.6LQARR210 pKa = 11.84LDD212 pKa = 3.98AKK214 pKa = 10.82PIGTPAQMRR223 pKa = 11.84AKK225 pKa = 10.42FSKK228 pKa = 10.06NAYY231 pKa = 9.81NSVTYY236 pKa = 9.91RR237 pKa = 11.84DD238 pKa = 4.04KK239 pKa = 11.59VNHH242 pKa = 4.89TRR244 pKa = 11.84KK245 pKa = 10.0LL246 pKa = 3.5

Molecular weight: 29.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

1313

117

624

328.3

38.04

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.864 ± 1.115

0.838 ± 0.357

6.626 ± 0.706

6.398 ± 1.05

5.255 ± 1.726

5.56 ± 0.982

1.98 ± 0.486

5.407 ± 0.298

6.093 ± 0.696

7.997 ± 0.832

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.58 ± 0.239

6.017 ± 0.826

4.341 ± 0.857

4.341 ± 0.409

6.626 ± 0.711

6.702 ± 0.326

4.798 ± 0.29

4.95 ± 0.545

0.99 ± 0.164

5.636 ± 0.818
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski