Cuiaba virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Rhabdoviridae; Alpharhabdovirinae; Sripuvirus; Cuiaba sripuvirus

Average proteome isoelectric point is 7.02

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3S8TMP8|A0A3S8TMP8_9RHAB U1 OS=Cuiaba virus OX=2495751 GN=U1 PE=4 SV=1
MM1 pKa = 7.38MSSQQSFPKK10 pKa = 10.7DD11 pKa = 3.33FDD13 pKa = 3.63PTKK16 pKa = 9.98TLEE19 pKa = 3.83AAKK22 pKa = 10.37RR23 pKa = 11.84GLKK26 pKa = 10.3KK27 pKa = 10.77LEE29 pKa = 4.6SDD31 pKa = 3.9DD32 pKa = 4.48KK33 pKa = 11.48MMFGVEE39 pKa = 4.12SLDD42 pKa = 4.44DD43 pKa = 4.78EE44 pKa = 4.66IEE46 pKa = 4.02KK47 pKa = 10.23HH48 pKa = 5.3QEE50 pKa = 3.74SSSDD54 pKa = 3.47EE55 pKa = 4.24EE56 pKa = 4.38EE57 pKa = 5.11EE58 pKa = 4.67EE59 pKa = 4.14EE60 pKa = 5.42CDD62 pKa = 4.47DD63 pKa = 4.1NVSKK67 pKa = 10.78EE68 pKa = 4.02AVPDD72 pKa = 3.97KK73 pKa = 10.74EE74 pKa = 4.13QLEE77 pKa = 4.22ADD79 pKa = 3.97DD80 pKa = 5.45FLEE83 pKa = 5.07QEE85 pKa = 4.58SSWTFNDD92 pKa = 2.91SKK94 pKa = 11.53LYY96 pKa = 7.45ITKK99 pKa = 10.08HH100 pKa = 5.0FSVNKK105 pKa = 9.31DD106 pKa = 2.94TMDD109 pKa = 3.81LVNAHH114 pKa = 6.32IRR116 pKa = 11.84NSVEE120 pKa = 3.49KK121 pKa = 9.64TLNFLGAKK129 pKa = 8.1ATYY132 pKa = 9.37EE133 pKa = 4.24GEE135 pKa = 4.54DD136 pKa = 3.56GSSFAWRR143 pKa = 11.84VSSDD147 pKa = 3.74AIHH150 pKa = 6.69PMEE153 pKa = 4.63KK154 pKa = 9.72HH155 pKa = 5.59YY156 pKa = 10.96VWQEE160 pKa = 3.47EE161 pKa = 4.34TQRR164 pKa = 11.84PIPSAPPSYY173 pKa = 10.86EE174 pKa = 4.77DD175 pKa = 4.56LDD177 pKa = 4.05LPSRR181 pKa = 11.84GKK183 pKa = 9.03TSPPLRR189 pKa = 11.84TSEE192 pKa = 4.16DD193 pKa = 3.39TTKK196 pKa = 10.7PDD198 pKa = 3.33YY199 pKa = 10.55QKK201 pKa = 10.8PSTSSEE207 pKa = 3.83CRR209 pKa = 11.84KK210 pKa = 10.24QSDD213 pKa = 3.48LSINRR218 pKa = 11.84EE219 pKa = 3.82FLDD222 pKa = 3.67SFKK225 pKa = 11.04KK226 pKa = 10.85GILFPSIDD234 pKa = 3.68GGGDD238 pKa = 3.18ILVKK242 pKa = 10.7LADD245 pKa = 3.76TDD247 pKa = 3.69LTEE250 pKa = 5.17AIIRR254 pKa = 11.84DD255 pKa = 4.04FVYY258 pKa = 10.61EE259 pKa = 4.33PSTSYY264 pKa = 11.39QDD266 pKa = 2.91MVYY269 pKa = 10.3QIFCIHH275 pKa = 6.95PQTKK279 pKa = 9.23PDD281 pKa = 3.43YY282 pKa = 11.1EE283 pKa = 4.54FIRR286 pKa = 11.84WW287 pKa = 3.37

Molecular weight: 32.92 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3S8TMQ4|A0A3S8TMQ4_9RHAB RNA-directed RNA polymerase L OS=Cuiaba virus OX=2495751 GN=L PE=3 SV=1
MM1 pKa = 7.75EE2 pKa = 4.53KK3 pKa = 9.68TGQKK7 pKa = 9.45PVSGQLSVQKK17 pKa = 10.87LIDD20 pKa = 3.4QGKK23 pKa = 7.59WEE25 pKa = 4.89PRR27 pKa = 11.84PPLNPAKK34 pKa = 10.31RR35 pKa = 11.84CTKK38 pKa = 10.33VEE40 pKa = 4.61DD41 pKa = 3.7LSSWPDD47 pKa = 3.14ITSKK51 pKa = 9.21TCSLFKK57 pKa = 10.78HH58 pKa = 6.18PLSFDD63 pKa = 3.16QGPDD67 pKa = 2.84AKK69 pKa = 10.22RR70 pKa = 11.84GKK72 pKa = 10.13KK73 pKa = 9.72ADD75 pKa = 4.45CIQIRR80 pKa = 11.84SPVFVAKK87 pKa = 9.92TIAQGDD93 pKa = 3.92NQLCMIPRR101 pKa = 4.12

Molecular weight: 11.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7

0

7

3742

101

2092

534.6

61.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.581 ± 0.444

2.218 ± 0.26

5.879 ± 0.816

6.708 ± 0.672

4.516 ± 0.203

5.692 ± 0.411

1.978 ± 0.334

7.803 ± 0.904

7.723 ± 0.584

9.246 ± 0.773

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.859 ± 0.258

4.784 ± 0.903

4.409 ± 0.559

3.18 ± 0.379

5.184 ± 0.37

8.311 ± 0.55

5.184 ± 0.542

5.158 ± 0.264

1.737 ± 0.204

3.848 ± 0.289
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski