Amino acid dipepetide frequency for Microbacterium phage Min1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
19.676AlaAla: 19.676 ± 1.674
1.141AlaCys: 1.141 ± 0.333
7.699AlaAsp: 7.699 ± 0.891
10.765AlaGlu: 10.765 ± 1.352
3.066AlaPhe: 3.066 ± 0.423
12.476AlaGly: 12.476 ± 1.152
1.854AlaHis: 1.854 ± 0.456
4.919AlaIle: 4.919 ± 0.543
3.208AlaLys: 3.208 ± 0.521
13.046AlaLeu: 13.046 ± 1.19
2.638AlaMet: 2.638 ± 0.475
3.137AlaAsn: 3.137 ± 0.5
6.202AlaPro: 6.202 ± 0.911
4.135AlaGln: 4.135 ± 0.634
11.549AlaArg: 11.549 ± 1.124
6.915AlaSer: 6.915 ± 1.092
7.343AlaThr: 7.343 ± 0.759
10.195AlaVal: 10.195 ± 0.894
2.78AlaTrp: 2.78 ± 0.373
1.854AlaTyr: 1.854 ± 0.326
0.0AlaXaa: 0.0 ± 0.0
Cys
0.784CysAla: 0.784 ± 0.241
0.285CysCys: 0.285 ± 0.137
0.285CysAsp: 0.285 ± 0.123
0.57CysGlu: 0.57 ± 0.237
0.071CysPhe: 0.071 ± 0.084
1.355CysGly: 1.355 ± 0.321
0.214CysHis: 0.214 ± 0.135
0.143CysIle: 0.143 ± 0.083
0.0CysLys: 0.0 ± 0.0
0.499CysLeu: 0.499 ± 0.246
0.0CysMet: 0.0 ± 0.0
0.285CysAsn: 0.285 ± 0.154
0.855CysPro: 0.855 ± 0.265
0.143CysGln: 0.143 ± 0.093
0.927CysArg: 0.927 ± 0.359
0.713CysSer: 0.713 ± 0.23
0.428CysThr: 0.428 ± 0.172
0.356CysVal: 0.356 ± 0.157
0.285CysTrp: 0.285 ± 0.153
0.143CysTyr: 0.143 ± 0.107
0.0CysXaa: 0.0 ± 0.0
Asp
9.553AspAla: 9.553 ± 1.033
0.356AspCys: 0.356 ± 0.154
4.206AspAsp: 4.206 ± 0.645
5.133AspGlu: 5.133 ± 0.63
0.855AspPhe: 0.855 ± 0.273
7.985AspGly: 7.985 ± 0.957
1.426AspHis: 1.426 ± 0.342
1.996AspIle: 1.996 ± 0.437
0.855AspLys: 0.855 ± 0.231
5.133AspLeu: 5.133 ± 0.822
0.784AspMet: 0.784 ± 0.218
0.855AspAsn: 0.855 ± 0.238
4.491AspPro: 4.491 ± 0.61
1.497AspGln: 1.497 ± 0.332
5.489AspArg: 5.489 ± 0.776
2.566AspSer: 2.566 ± 0.502
2.852AspThr: 2.852 ± 0.548
5.204AspVal: 5.204 ± 0.704
1.212AspTrp: 1.212 ± 0.24
1.069AspTyr: 1.069 ± 0.27
0.0AspXaa: 0.0 ± 0.0
Glu
7.699GluAla: 7.699 ± 0.949
0.356GluCys: 0.356 ± 0.154
3.778GluAsp: 3.778 ± 0.472
3.351GluGlu: 3.351 ± 0.486
1.283GluPhe: 1.283 ± 0.332
5.204GluGly: 5.204 ± 0.619
2.709GluHis: 2.709 ± 0.541
2.067GluIle: 2.067 ± 0.428
2.353GluLys: 2.353 ± 0.44
5.418GluLeu: 5.418 ± 0.64
1.069GluMet: 1.069 ± 0.22
1.283GluAsn: 1.283 ± 0.255
3.493GluPro: 3.493 ± 0.554
2.994GluGln: 2.994 ± 0.599
6.131GluArg: 6.131 ± 0.78
2.424GluSer: 2.424 ± 0.434
3.493GluThr: 3.493 ± 0.525
5.133GluVal: 5.133 ± 0.511
1.212GluTrp: 1.212 ± 0.349
1.64GluTyr: 1.64 ± 0.43
0.0GluXaa: 0.0 ± 0.0
Phe
2.353PheAla: 2.353 ± 0.429
0.071PheCys: 0.071 ± 0.073
2.067PheAsp: 2.067 ± 0.395
1.355PheGlu: 1.355 ± 0.298
0.713PhePhe: 0.713 ± 0.221
1.782PheGly: 1.782 ± 0.449
0.143PheHis: 0.143 ± 0.1
0.713PheIle: 0.713 ± 0.303
0.642PheLys: 0.642 ± 0.22
1.711PheLeu: 1.711 ± 0.336
0.214PheMet: 0.214 ± 0.126
0.356PheAsn: 0.356 ± 0.139
1.283PhePro: 1.283 ± 0.413
0.57PheGln: 0.57 ± 0.147
1.426PheArg: 1.426 ± 0.296
1.283PheSer: 1.283 ± 0.31
2.495PheThr: 2.495 ± 0.393
2.139PheVal: 2.139 ± 0.445
0.428PheTrp: 0.428 ± 0.17
0.713PheTyr: 0.713 ± 0.237
0.0PheXaa: 0.0 ± 0.0
Gly
11.62GlyAla: 11.62 ± 1.125
0.784GlyCys: 0.784 ± 0.326
5.489GlyAsp: 5.489 ± 0.772
6.559GlyGlu: 6.559 ± 0.759
2.067GlyPhe: 2.067 ± 0.361
7.2GlyGly: 7.2 ± 1.021
1.996GlyHis: 1.996 ± 0.379
4.064GlyIle: 4.064 ± 0.606
2.067GlyLys: 2.067 ± 0.395
6.844GlyLeu: 6.844 ± 0.92
1.854GlyMet: 1.854 ± 0.396
2.067GlyAsn: 2.067 ± 0.37
3.992GlyPro: 3.992 ± 0.6
2.566GlyGln: 2.566 ± 0.486
7.699GlyArg: 7.699 ± 0.716
4.705GlySer: 4.705 ± 0.725
5.133GlyThr: 5.133 ± 0.645
8.911GlyVal: 8.911 ± 0.882
2.424GlyTrp: 2.424 ± 0.481
1.711GlyTyr: 1.711 ± 0.399
0.0GlyXaa: 0.0 ± 0.0
His
2.566HisAla: 2.566 ± 0.564
0.428HisCys: 0.428 ± 0.186
1.426HisAsp: 1.426 ± 0.273
0.998HisGlu: 0.998 ± 0.255
0.356HisPhe: 0.356 ± 0.162
1.64HisGly: 1.64 ± 0.326
0.713HisHis: 0.713 ± 0.304
0.998HisIle: 0.998 ± 0.338
0.642HisLys: 0.642 ± 0.313
1.996HisLeu: 1.996 ± 0.427
0.428HisMet: 0.428 ± 0.213
0.57HisAsn: 0.57 ± 0.156
1.497HisPro: 1.497 ± 0.341
0.499HisGln: 0.499 ± 0.18
2.424HisArg: 2.424 ± 0.424
1.069HisSer: 1.069 ± 0.287
0.784HisThr: 0.784 ± 0.221
1.711HisVal: 1.711 ± 0.335
0.143HisTrp: 0.143 ± 0.121
0.071HisTyr: 0.071 ± 0.073
0.0HisXaa: 0.0 ± 0.0
Ile
5.489IleAla: 5.489 ± 0.679
0.071IleCys: 0.071 ± 0.075
3.565IleAsp: 3.565 ± 0.506
3.493IleGlu: 3.493 ± 0.626
0.499IlePhe: 0.499 ± 0.198
2.78IleGly: 2.78 ± 0.529
0.855IleHis: 0.855 ± 0.218
1.497IleIle: 1.497 ± 0.313
0.499IleLys: 0.499 ± 0.161
3.565IleLeu: 3.565 ± 0.515
0.499IleMet: 0.499 ± 0.164
0.713IleAsn: 0.713 ± 0.231
2.21IlePro: 2.21 ± 0.473
1.283IleGln: 1.283 ± 0.413
2.923IleArg: 2.923 ± 0.485
1.996IleSer: 1.996 ± 0.367
3.493IleThr: 3.493 ± 0.6
3.636IleVal: 3.636 ± 0.63
0.214IleTrp: 0.214 ± 0.114
0.713IleTyr: 0.713 ± 0.179
0.0IleXaa: 0.0 ± 0.0
Lys
3.565LysAla: 3.565 ± 0.554
0.0LysCys: 0.0 ± 0.0
1.711LysAsp: 1.711 ± 0.357
1.497LysGlu: 1.497 ± 0.287
0.499LysPhe: 0.499 ± 0.17
1.497LysGly: 1.497 ± 0.4
0.428LysHis: 0.428 ± 0.185
0.784LysIle: 0.784 ± 0.228
0.642LysLys: 0.642 ± 0.25
2.495LysLeu: 2.495 ± 0.367
0.214LysMet: 0.214 ± 0.119
0.57LysAsn: 0.57 ± 0.167
0.855LysPro: 0.855 ± 0.307
0.713LysGln: 0.713 ± 0.267
2.067LysArg: 2.067 ± 0.434
1.64LysSer: 1.64 ± 0.372
1.426LysThr: 1.426 ± 0.358
2.566LysVal: 2.566 ± 0.406
0.285LysTrp: 0.285 ± 0.121
0.499LysTyr: 0.499 ± 0.21
0.0LysXaa: 0.0 ± 0.0
Leu
12.975LeuAla: 12.975 ± 1.05
0.855LeuCys: 0.855 ± 0.231
5.133LeuAsp: 5.133 ± 0.511
3.565LeuGlu: 3.565 ± 0.517
2.21LeuPhe: 2.21 ± 0.354
8.698LeuGly: 8.698 ± 1.114
1.283LeuHis: 1.283 ± 0.314
4.919LeuIle: 4.919 ± 0.804
1.711LeuLys: 1.711 ± 0.393
6.844LeuLeu: 6.844 ± 0.853
1.497LeuMet: 1.497 ± 0.333
1.141LeuAsn: 1.141 ± 0.341
5.489LeuPro: 5.489 ± 0.73
2.21LeuGln: 2.21 ± 0.377
7.913LeuArg: 7.913 ± 0.727
4.777LeuSer: 4.777 ± 0.635
4.919LeuThr: 4.919 ± 0.659
6.987LeuVal: 6.987 ± 0.806
0.713LeuTrp: 0.713 ± 0.289
1.497LeuTyr: 1.497 ± 0.321
0.0LeuXaa: 0.0 ± 0.0
Met
2.495MetAla: 2.495 ± 0.363
0.071MetCys: 0.071 ± 0.089
0.855MetAsp: 0.855 ± 0.208
0.855MetGlu: 0.855 ± 0.248
0.143MetPhe: 0.143 ± 0.095
1.64MetGly: 1.64 ± 0.309
0.285MetHis: 0.285 ± 0.141
0.713MetIle: 0.713 ± 0.201
0.642MetLys: 0.642 ± 0.22
1.64MetLeu: 1.64 ± 0.316
0.428MetMet: 0.428 ± 0.165
0.713MetAsn: 0.713 ± 0.287
0.713MetPro: 0.713 ± 0.304
0.713MetGln: 0.713 ± 0.203
1.355MetArg: 1.355 ± 0.317
1.426MetSer: 1.426 ± 0.317
1.568MetThr: 1.568 ± 0.275
0.784MetVal: 0.784 ± 0.197
0.428MetTrp: 0.428 ± 0.164
0.285MetTyr: 0.285 ± 0.143
0.0MetXaa: 0.0 ± 0.0
Asn
2.21AsnAla: 2.21 ± 0.332
0.214AsnCys: 0.214 ± 0.115
1.283AsnAsp: 1.283 ± 0.288
0.855AsnGlu: 0.855 ± 0.273
0.713AsnPhe: 0.713 ± 0.191
2.424AsnGly: 2.424 ± 0.484
0.285AsnHis: 0.285 ± 0.165
0.784AsnIle: 0.784 ± 0.24
0.356AsnLys: 0.356 ± 0.127
1.069AsnLeu: 1.069 ± 0.26
0.143AsnMet: 0.143 ± 0.104
0.642AsnAsn: 0.642 ± 0.206
1.497AsnPro: 1.497 ± 0.321
0.428AsnGln: 0.428 ± 0.186
1.497AsnArg: 1.497 ± 0.398
0.998AsnSer: 0.998 ± 0.287
0.927AsnThr: 0.927 ± 0.277
2.281AsnVal: 2.281 ± 0.428
0.499AsnTrp: 0.499 ± 0.243
0.214AsnTyr: 0.214 ± 0.123
0.0AsnXaa: 0.0 ± 0.0
Pro
6.773ProAla: 6.773 ± 0.78
0.428ProCys: 0.428 ± 0.165
4.848ProAsp: 4.848 ± 0.753
3.707ProGlu: 3.707 ± 0.553
1.212ProPhe: 1.212 ± 0.299
3.565ProGly: 3.565 ± 0.658
1.283ProHis: 1.283 ± 0.269
2.495ProIle: 2.495 ± 0.389
1.069ProLys: 1.069 ± 0.275
4.563ProLeu: 4.563 ± 0.614
0.927ProMet: 0.927 ± 0.211
0.927ProAsn: 0.927 ± 0.277
3.493ProPro: 3.493 ± 0.594
1.711ProGln: 1.711 ± 0.428
3.778ProArg: 3.778 ± 0.629
3.921ProSer: 3.921 ± 0.517
4.42ProThr: 4.42 ± 0.764
5.347ProVal: 5.347 ± 0.593
1.212ProTrp: 1.212 ± 0.297
0.927ProTyr: 0.927 ± 0.218
0.0ProXaa: 0.0 ± 0.0
Gln
3.85GlnAla: 3.85 ± 0.522
0.143GlnCys: 0.143 ± 0.088
1.283GlnAsp: 1.283 ± 0.255
1.64GlnGlu: 1.64 ± 0.357
0.784GlnPhe: 0.784 ± 0.211
3.707GlnGly: 3.707 ± 0.415
0.57GlnHis: 0.57 ± 0.195
1.355GlnIle: 1.355 ± 0.28
0.713GlnLys: 0.713 ± 0.266
2.78GlnLeu: 2.78 ± 0.413
0.713GlnMet: 0.713 ± 0.203
0.499GlnAsn: 0.499 ± 0.186
1.64GlnPro: 1.64 ± 0.33
1.069GlnGln: 1.069 ± 0.267
2.067GlnArg: 2.067 ± 0.435
1.212GlnSer: 1.212 ± 0.264
2.495GlnThr: 2.495 ± 0.378
2.78GlnVal: 2.78 ± 0.369
0.642GlnTrp: 0.642 ± 0.23
0.642GlnTyr: 0.642 ± 0.179
0.0GlnXaa: 0.0 ± 0.0
Arg
11.121ArgAla: 11.121 ± 1.253
0.998ArgCys: 0.998 ± 0.246
5.917ArgAsp: 5.917 ± 0.711
5.204ArgGlu: 5.204 ± 0.568
2.067ArgPhe: 2.067 ± 0.424
6.131ArgGly: 6.131 ± 0.847
2.852ArgHis: 2.852 ± 0.55
3.422ArgIle: 3.422 ± 0.431
1.782ArgLys: 1.782 ± 0.377
6.345ArgLeu: 6.345 ± 0.816
1.996ArgMet: 1.996 ± 0.333
1.711ArgAsn: 1.711 ± 0.366
4.42ArgPro: 4.42 ± 0.632
2.566ArgGln: 2.566 ± 0.505
8.983ArgArg: 8.983 ± 1.136
4.349ArgSer: 4.349 ± 0.533
5.418ArgThr: 5.418 ± 0.533
6.701ArgVal: 6.701 ± 0.862
1.497ArgTrp: 1.497 ± 0.367
2.281ArgTyr: 2.281 ± 0.493
0.0ArgXaa: 0.0 ± 0.0
Ser
6.844SerAla: 6.844 ± 0.95
0.428SerCys: 0.428 ± 0.212
2.709SerAsp: 2.709 ± 0.481
3.066SerGlu: 3.066 ± 0.561
1.64SerPhe: 1.64 ± 0.402
5.204SerGly: 5.204 ± 0.759
0.784SerHis: 0.784 ± 0.259
1.711SerIle: 1.711 ± 0.366
1.283SerLys: 1.283 ± 0.334
4.777SerLeu: 4.777 ± 0.566
1.996SerMet: 1.996 ± 0.346
1.069SerAsn: 1.069 ± 0.313
2.709SerPro: 2.709 ± 0.451
1.212SerGln: 1.212 ± 0.267
4.705SerArg: 4.705 ± 0.556
3.066SerSer: 3.066 ± 0.511
3.707SerThr: 3.707 ± 0.59
4.135SerVal: 4.135 ± 0.61
1.568SerTrp: 1.568 ± 0.329
1.141SerTyr: 1.141 ± 0.33
0.0SerXaa: 0.0 ± 0.0
Thr
8.84ThrAla: 8.84 ± 0.888
0.713ThrCys: 0.713 ± 0.286
3.778ThrAsp: 3.778 ± 0.551
3.422ThrGlu: 3.422 ± 0.492
1.854ThrPhe: 1.854 ± 0.408
6.487ThrGly: 6.487 ± 0.802
0.713ThrHis: 0.713 ± 0.223
3.493ThrIle: 3.493 ± 0.514
2.067ThrLys: 2.067 ± 0.387
5.703ThrLeu: 5.703 ± 0.59
0.57ThrMet: 0.57 ± 0.185
1.069ThrAsn: 1.069 ± 0.277
4.42ThrPro: 4.42 ± 0.788
1.497ThrGln: 1.497 ± 0.332
4.349ThrArg: 4.349 ± 0.643
2.638ThrSer: 2.638 ± 0.56
3.636ThrThr: 3.636 ± 0.596
5.632ThrVal: 5.632 ± 0.764
1.283ThrTrp: 1.283 ± 0.264
1.212ThrTyr: 1.212 ± 0.323
0.0ThrXaa: 0.0 ± 0.0
Val
11.62ValAla: 11.62 ± 1.143
0.927ValCys: 0.927 ± 0.284
4.919ValAsp: 4.919 ± 0.607
5.276ValGlu: 5.276 ± 0.758
1.64ValPhe: 1.64 ± 0.323
7.2ValGly: 7.2 ± 0.973
1.996ValHis: 1.996 ± 0.483
2.994ValIle: 2.994 ± 0.374
1.782ValLys: 1.782 ± 0.387
6.915ValLeu: 6.915 ± 0.709
1.426ValMet: 1.426 ± 0.31
1.069ValAsn: 1.069 ± 0.329
5.276ValPro: 5.276 ± 0.504
2.923ValGln: 2.923 ± 0.416
7.414ValArg: 7.414 ± 0.78
5.632ValSer: 5.632 ± 0.781
5.917ValThr: 5.917 ± 0.825
5.561ValVal: 5.561 ± 0.78
1.497ValTrp: 1.497 ± 0.371
1.141ValTyr: 1.141 ± 0.275
0.0ValXaa: 0.0 ± 0.0
Trp
2.495TrpAla: 2.495 ± 0.439
0.0TrpCys: 0.0 ± 0.0
1.355TrpAsp: 1.355 ± 0.283
0.855TrpGlu: 0.855 ± 0.279
0.499TrpPhe: 0.499 ± 0.18
1.283TrpGly: 1.283 ± 0.342
0.428TrpHis: 0.428 ± 0.2
0.499TrpIle: 0.499 ± 0.163
0.998TrpLys: 0.998 ± 0.234
2.281TrpLeu: 2.281 ± 0.351
0.214TrpMet: 0.214 ± 0.127
0.428TrpAsn: 0.428 ± 0.166
0.784TrpPro: 0.784 ± 0.26
0.855TrpGln: 0.855 ± 0.248
1.283TrpArg: 1.283 ± 0.298
1.497TrpSer: 1.497 ± 0.338
1.069TrpThr: 1.069 ± 0.316
1.426TrpVal: 1.426 ± 0.363
0.642TrpTrp: 0.642 ± 0.218
0.356TrpTyr: 0.356 ± 0.175
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.067TyrAla: 2.067 ± 0.379
0.071TyrCys: 0.071 ± 0.064
1.212TyrAsp: 1.212 ± 0.306
0.927TyrGlu: 0.927 ± 0.287
0.285TyrPhe: 0.285 ± 0.149
1.497TyrGly: 1.497 ± 0.351
0.214TyrHis: 0.214 ± 0.142
0.428TyrIle: 0.428 ± 0.167
0.642TyrLys: 0.642 ± 0.249
1.711TyrLeu: 1.711 ± 0.302
0.071TyrMet: 0.071 ± 0.07
0.214TyrAsn: 0.214 ± 0.119
1.283TyrPro: 1.283 ± 0.287
0.998TyrGln: 0.998 ± 0.227
1.782TyrArg: 1.782 ± 0.414
0.927TyrSer: 0.927 ± 0.235
1.854TyrThr: 1.854 ± 0.396
1.64TyrVal: 1.64 ± 0.316
0.285TyrTrp: 0.285 ± 0.196
0.356TyrTyr: 0.356 ± 0.151
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 77 proteins (14028 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski