Pseudospirillum japonicum

Taxonomy: cellular organisms; Bacteria;

Average proteome isoelectric point is 6.26

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2385 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H6R4W0|A0A1H6R4W0_9GAMM Cytochrome c-type biogenesis protein CcmE OS=Pseudospirillum japonicum OX=64971 GN=ccmE PE=3 SV=1
MM1 pKa = 7.29SVVAQNFTTYY11 pKa = 10.4VYY13 pKa = 11.03NQLLKK18 pKa = 10.67RR19 pKa = 11.84NPDD22 pKa = 3.68AEE24 pKa = 4.1GLTYY28 pKa = 10.05WEE30 pKa = 4.25QQLDD34 pKa = 3.97STQVSAEE41 pKa = 3.87DD42 pKa = 3.28MFVYY46 pKa = 10.41FLEE49 pKa = 4.47SPEE52 pKa = 3.93FSDD55 pKa = 4.19RR56 pKa = 11.84YY57 pKa = 10.42SPIIRR62 pKa = 11.84LYY64 pKa = 8.79MSAFNRR70 pKa = 11.84FPDD73 pKa = 4.06SEE75 pKa = 4.0GMEE78 pKa = 4.04YY79 pKa = 9.75WAQEE83 pKa = 4.04MANGQTPYY91 pKa = 10.8QIARR95 pKa = 11.84LLLSSTEE102 pKa = 3.94YY103 pKa = 10.57TDD105 pKa = 3.65MYY107 pKa = 10.79GSDD110 pKa = 3.41VSHH113 pKa = 6.99SDD115 pKa = 3.58FVNSLYY121 pKa = 11.35VNVLGRR127 pKa = 11.84AGDD130 pKa = 3.9PSGTAYY136 pKa = 9.67WEE138 pKa = 4.12EE139 pKa = 4.48VLTQGLATQKK149 pKa = 10.74DD150 pKa = 4.02VLVSFSEE157 pKa = 4.11SQEE160 pKa = 4.33AIEE163 pKa = 5.16GMAKK167 pKa = 9.9EE168 pKa = 4.41VNTVLAYY175 pKa = 10.68NAMLGRR181 pKa = 11.84EE182 pKa = 4.19PTAEE186 pKa = 4.11EE187 pKa = 4.14LRR189 pKa = 11.84RR190 pKa = 11.84APSDD194 pKa = 3.3PAILVSQLFQGTEE207 pKa = 4.0YY208 pKa = 10.84NGPLVPGYY216 pKa = 9.44EE217 pKa = 3.85LEE219 pKa = 4.35YY220 pKa = 10.48WVDD223 pKa = 3.66TQVLGDD229 pKa = 3.85TLYY232 pKa = 10.27LTDD235 pKa = 4.0KK236 pKa = 10.63SHH238 pKa = 6.6GVVLADD244 pKa = 4.09LRR246 pKa = 11.84EE247 pKa = 4.32DD248 pKa = 3.5QVTDD252 pKa = 2.98AGTVLNVQDD261 pKa = 4.51LVSAVNIDD269 pKa = 3.49ASDD272 pKa = 3.4MRR274 pKa = 11.84FNAARR279 pKa = 11.84LYY281 pKa = 11.31GDD283 pKa = 4.96DD284 pKa = 4.99DD285 pKa = 4.88ANKK288 pKa = 8.92LTATGAADD296 pKa = 3.28ILYY299 pKa = 10.3GRR301 pKa = 11.84AGNDD305 pKa = 3.27LLEE308 pKa = 4.58GLSGNDD314 pKa = 3.16TLYY317 pKa = 11.32GDD319 pKa = 4.23EE320 pKa = 5.35GNDD323 pKa = 3.82TLHH326 pKa = 6.73GGYY329 pKa = 10.69GEE331 pKa = 4.85DD332 pKa = 4.48RR333 pKa = 11.84MFGGDD338 pKa = 3.73GNDD341 pKa = 3.28EE342 pKa = 4.64FIIADD347 pKa = 3.75LDD349 pKa = 4.41EE350 pKa = 4.28IHH352 pKa = 7.09LEE354 pKa = 4.04QEE356 pKa = 4.31EE357 pKa = 4.38LQGGAGTDD365 pKa = 2.91RR366 pKa = 11.84LTLQEE371 pKa = 4.44SGAVDD376 pKa = 4.07LSKK379 pKa = 11.41VLMFDD384 pKa = 3.4MEE386 pKa = 4.42EE387 pKa = 4.73LEE389 pKa = 4.79LSNGGNTVTMVRR401 pKa = 11.84EE402 pKa = 3.92QLLQLTNIVPAAQGDD417 pKa = 4.64SILKK421 pKa = 9.47LAQAATLDD429 pKa = 4.14LEE431 pKa = 5.17QMPQLQAFNKK441 pKa = 9.91IYY443 pKa = 10.68GATTGSNDD451 pKa = 3.38LLGYY455 pKa = 10.78AGDD458 pKa = 4.7DD459 pKa = 3.57YY460 pKa = 11.88LVGGKK465 pKa = 9.44NEE467 pKa = 3.9DD468 pKa = 3.44QLRR471 pKa = 11.84GGEE474 pKa = 4.18GVDD477 pKa = 3.52YY478 pKa = 11.56LEE480 pKa = 5.03GGEE483 pKa = 4.27GADD486 pKa = 2.46IYY488 pKa = 11.47VFNRR492 pKa = 11.84ADD494 pKa = 3.45YY495 pKa = 9.37SQQSVGATDD504 pKa = 4.05FVGDD508 pKa = 4.66TIHH511 pKa = 7.17GLDD514 pKa = 3.74FTQGDD519 pKa = 4.56KK520 pKa = 10.71IRR522 pKa = 11.84LEE524 pKa = 4.34APLSGSTQALSLEE537 pKa = 4.26SGFYY541 pKa = 10.61SSLTEE546 pKa = 4.38TIQANATIQSAFAGDD561 pKa = 4.15DD562 pKa = 3.17VDD564 pKa = 5.73AVLLQVKK571 pKa = 10.02AGTAAGYY578 pKa = 9.36YY579 pKa = 10.54LLVEE583 pKa = 5.08EE584 pKa = 5.11YY585 pKa = 10.81NADD588 pKa = 3.84YY589 pKa = 11.06QPADD593 pKa = 3.62ATDD596 pKa = 3.72TTDD599 pKa = 4.36DD600 pKa = 3.81AASLVNTEE608 pKa = 4.28TDD610 pKa = 3.28TLNYY614 pKa = 10.6DD615 pKa = 3.64PASDD619 pKa = 4.95LIIRR623 pKa = 11.84LTGIEE628 pKa = 5.01SLNQLNGFSTSVFII642 pKa = 5.76

Molecular weight: 69.84 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H6U170|A0A1H6U170_9GAMM Acetyl-CoA C-acetyltransferase OS=Pseudospirillum japonicum OX=64971 GN=SAMN05421831_11252 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLKK11 pKa = 9.97RR12 pKa = 11.84KK13 pKa = 8.91RR14 pKa = 11.84VHH16 pKa = 6.29GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.4NGRR28 pKa = 11.84QVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.93GRR39 pKa = 11.84KK40 pKa = 8.85RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2385

0

2385

820087

31

1776

343.9

38.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.38 ± 0.055

1.029 ± 0.017

4.964 ± 0.036

5.909 ± 0.049

3.491 ± 0.03

6.437 ± 0.05

2.777 ± 0.029

5.503 ± 0.035

3.881 ± 0.042

11.777 ± 0.064

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.273 ± 0.022

3.086 ± 0.03

4.423 ± 0.037

7.071 ± 0.069

5.289 ± 0.038

5.526 ± 0.04

5.333 ± 0.044

6.397 ± 0.039

1.443 ± 0.023

3.011 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski