Amino acid dipepetide frequency for Clostridium sp. CAG:1193

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	1.853AlaAla: 1.853 ± 0.112	0.55AlaCys: 0.55 ± 0.049	1.866AlaAsp: 1.866 ± 0.08	2.203AlaGlu: 2.203 ± 0.102	2.099AlaPhe: 2.099 ± 0.087	2.377AlaGly: 2.377 ± 0.102	0.653AlaHis: 0.653 ± 0.043	4.202AlaIle: 4.202 ± 0.124	3.998AlaLys: 3.998 ± 0.147	4.58AlaLeu: 4.58 ± 0.134	1.032AlaMet: 1.032 ± 0.059	2.452AlaAsn: 2.452 ± 0.094	0.935AlaPro: 0.935 ± 0.056	0.689AlaGln: 0.689 ± 0.049	1.604AlaArg: 1.604 ± 0.083	3.196AlaSer: 3.196 ± 0.112	2.691AlaThr: 2.691 ± 0.138	2.607AlaVal: 2.607 ± 0.114	0.243AlaTrp: 0.243 ± 0.028	1.934AlaTyr: 1.934 ± 0.077	0.0AlaXaa: 0.0 ± 0.0
Cys	0.579CysAla: 0.579 ± 0.045	0.158CysCys: 0.158 ± 0.027	0.776CysAsp: 0.776 ± 0.059	0.734CysGlu: 0.734 ± 0.058	0.42CysPhe: 0.42 ± 0.038	0.951CysGly: 0.951 ± 0.063	0.146CysHis: 0.146 ± 0.02	1.064CysIle: 1.064 ± 0.07	1.025CysLys: 1.025 ± 0.068	0.731CysLeu: 0.731 ± 0.051	0.275CysMet: 0.275 ± 0.032	0.825CysAsn: 0.825 ± 0.061	0.359CysPro: 0.359 ± 0.041	0.152CysGln: 0.152 ± 0.022	0.298CysArg: 0.298 ± 0.031	0.647CysSer: 0.647 ± 0.055	0.64CysThr: 0.64 ± 0.053	0.734CysVal: 0.734 ± 0.048	0.049CysTrp: 0.049 ± 0.014	0.576CysTyr: 0.576 ± 0.044	0.0CysXaa: 0.0 ± 0.0
Asp	2.927AspAla: 2.927 ± 0.11	0.42AspCys: 0.42 ± 0.043	3.636AspAsp: 3.636 ± 0.146	5.631AspGlu: 5.631 ± 0.154	2.675AspPhe: 2.675 ± 0.101	3.218AspGly: 3.218 ± 0.124	0.482AspHis: 0.482 ± 0.041	7.012AspIle: 7.012 ± 0.171	6.602AspLys: 6.602 ± 0.159	5.415AspLeu: 5.415 ± 0.146	1.724AspMet: 1.724 ± 0.09	4.661AspAsn: 4.661 ± 0.144	1.213AspPro: 1.213 ± 0.066	0.608AspGln: 0.608 ± 0.043	1.556AspArg: 1.556 ± 0.076	3.435AspSer: 3.435 ± 0.115	3.506AspThr: 3.506 ± 0.126	3.969AspVal: 3.969 ± 0.11	0.294AspTrp: 0.294 ± 0.03	3.448AspTyr: 3.448 ± 0.119	0.003AspXaa: 0.003 ± 0.004
Glu	3.082GluAla: 3.082 ± 0.122	0.741GluCys: 0.741 ± 0.05	4.253GluAsp: 4.253 ± 0.145	5.932GluGlu: 5.932 ± 0.21	2.966GluPhe: 2.966 ± 0.11	3.086GluGly: 3.086 ± 0.104	0.786GluHis: 0.786 ± 0.058	7.21GluIle: 7.21 ± 0.202	7.523GluLys: 7.523 ± 0.229	6.835GluLeu: 6.835 ± 0.179	2.012GluMet: 2.012 ± 0.078	5.353GluAsn: 5.353 ± 0.144	1.249GluPro: 1.249 ± 0.067	1.307GluGln: 1.307 ± 0.07	2.403GluArg: 2.403 ± 0.114	3.891GluSer: 3.891 ± 0.123	2.927GluThr: 2.927 ± 0.112	4.713GluVal: 4.713 ± 0.134	0.372GluTrp: 0.372 ± 0.035	4.04GluTyr: 4.04 ± 0.132	0.006GluXaa: 0.006 ± 0.004
Phe	1.792PheAla: 1.792 ± 0.085	0.424PheCys: 0.424 ± 0.034	3.17PheAsp: 3.17 ± 0.134	2.394PheGlu: 2.394 ± 0.094	1.465PhePhe: 1.465 ± 0.085	2.326PheGly: 2.326 ± 0.103	0.446PheHis: 0.446 ± 0.036	4.506PheIle: 4.506 ± 0.175	4.079PheLys: 4.079 ± 0.128	3.571PheLeu: 3.571 ± 0.132	0.99PheMet: 0.99 ± 0.059	3.351PheAsn: 3.351 ± 0.124	1.051PhePro: 1.051 ± 0.06	0.627PheGln: 0.627 ± 0.045	1.09PheArg: 1.09 ± 0.066	2.93PheSer: 2.93 ± 0.117	2.358PheThr: 2.358 ± 0.09	2.478PheVal: 2.478 ± 0.102	0.201PheTrp: 0.201 ± 0.027	2.002PheTyr: 2.002 ± 0.083	0.0PheXaa: 0.0 ± 0.0
Gly	2.549GlyAla: 2.549 ± 0.126	0.763GlyCys: 0.763 ± 0.053	2.827GlyAsp: 2.827 ± 0.123	3.137GlyGlu: 3.137 ± 0.117	2.277GlyPhe: 2.277 ± 0.097	3.26GlyGly: 3.26 ± 0.251	0.889GlyHis: 0.889 ± 0.064	5.796GlyIle: 5.796 ± 0.14	4.952GlyLys: 4.952 ± 0.155	4.389GlyLeu: 4.389 ± 0.121	1.439GlyMet: 1.439 ± 0.071	3.396GlyAsn: 3.396 ± 0.186	0.909GlyPro: 0.909 ± 0.048	0.809GlyGln: 0.809 ± 0.049	1.76GlyArg: 1.76 ± 0.075	3.574GlySer: 3.574 ± 0.164	3.073GlyThr: 3.073 ± 0.15	3.826GlyVal: 3.826 ± 0.116	0.327GlyTrp: 0.327 ± 0.042	3.364GlyTyr: 3.364 ± 0.143	0.006GlyXaa: 0.006 ± 0.005
His	0.631HisAla: 0.631 ± 0.05	0.113HisCys: 0.113 ± 0.024	0.634HisAsp: 0.634 ± 0.048	0.792HisGlu: 0.792 ± 0.051	0.547HisPhe: 0.547 ± 0.045	0.744HisGly: 0.744 ± 0.059	0.243HisHis: 0.243 ± 0.031	1.249HisIle: 1.249 ± 0.058	0.902HisLys: 0.902 ± 0.052	1.171HisLeu: 1.171 ± 0.068	0.307HisMet: 0.307 ± 0.032	0.851HisAsn: 0.851 ± 0.05	0.501HisPro: 0.501 ± 0.044	0.243HisGln: 0.243 ± 0.031	0.398HisArg: 0.398 ± 0.037	0.728HisSer: 0.728 ± 0.045	0.637HisThr: 0.637 ± 0.05	0.682HisVal: 0.682 ± 0.048	0.052HisTrp: 0.052 ± 0.013	0.598HisTyr: 0.598 ± 0.047	0.0HisXaa: 0.0 ± 0.0
Ile	4.263IleAla: 4.263 ± 0.115	1.206IleCys: 1.206 ± 0.057	7.323IleAsp: 7.323 ± 0.159	6.928IleGlu: 6.928 ± 0.182	4.072IlePhe: 4.072 ± 0.151	5.185IleGly: 5.185 ± 0.149	1.132IleHis: 1.132 ± 0.065	10.697IleIle: 10.697 ± 0.305	11.075IleLys: 11.075 ± 0.215	9.393IleLeu: 9.393 ± 0.266	2.17IleMet: 2.17 ± 0.083	8.565IleAsn: 8.565 ± 0.231	3.044IlePro: 3.044 ± 0.107	1.368IleGln: 1.368 ± 0.072	2.811IleArg: 2.811 ± 0.097	7.161IleSer: 7.161 ± 0.167	5.832IleThr: 5.832 ± 0.148	6.508IleVal: 6.508 ± 0.161	0.411IleTrp: 0.411 ± 0.037	4.787IleTyr: 4.787 ± 0.163	0.006IleXaa: 0.006 ± 0.004
Lys	3.374LysAla: 3.374 ± 0.128	1.168LysCys: 1.168 ± 0.081	7.436LysAsp: 7.436 ± 0.172	10.787LysGlu: 10.787 ± 0.248	3.128LysPhe: 3.128 ± 0.104	4.583LysGly: 4.583 ± 0.143	0.97LysHis: 0.97 ± 0.054	9.7LysIle: 9.7 ± 0.196	10.535LysLys: 10.535 ± 0.215	8.128LysLeu: 8.128 ± 0.187	2.804LysMet: 2.804 ± 0.087	8.339LysAsn: 8.339 ± 0.2	1.747LysPro: 1.747 ± 0.069	1.834LysGln: 1.834 ± 0.076	3.545LysArg: 3.545 ± 0.103	5.408LysSer: 5.408 ± 0.129	4.955LysThr: 4.955 ± 0.15	6.369LysVal: 6.369 ± 0.169	0.553LysTrp: 0.553 ± 0.044	5.977LysTyr: 5.977 ± 0.191	0.0LysXaa: 0.0 ± 0.0
Leu	3.467LeuAla: 3.467 ± 0.121	0.97LeuCys: 0.97 ± 0.058	5.732LeuAsp: 5.732 ± 0.164	5.757LeuGlu: 5.757 ± 0.197	4.143LeuPhe: 4.143 ± 0.169	4.706LeuGly: 4.706 ± 0.131	0.944LeuHis: 0.944 ± 0.061	9.296LeuIle: 9.296 ± 0.236	9.154LeuLys: 9.154 ± 0.189	8.348LeuLeu: 8.348 ± 0.189	2.041LeuMet: 2.041 ± 0.083	7.097LeuAsn: 7.097 ± 0.173	2.245LeuPro: 2.245 ± 0.083	1.462LeuGln: 1.462 ± 0.08	2.662LeuArg: 2.662 ± 0.115	7.139LeuSer: 7.139 ± 0.215	4.884LeuThr: 4.884 ± 0.132	5.505LeuVal: 5.505 ± 0.141	0.427LeuTrp: 0.427 ± 0.037	4.124LeuTyr: 4.124 ± 0.123	0.0LeuXaa: 0.0 ± 0.0
Met	1.142MetAla: 1.142 ± 0.06	0.272MetCys: 0.272 ± 0.035	1.514MetAsp: 1.514 ± 0.068	1.53MetGlu: 1.53 ± 0.079	1.462MetPhe: 1.462 ± 0.124	1.423MetGly: 1.423 ± 0.077	0.356MetHis: 0.356 ± 0.036	2.4MetIle: 2.4 ± 0.099	2.484MetLys: 2.484 ± 0.086	2.232MetLeu: 2.232 ± 0.091	0.605MetMet: 0.605 ± 0.045	1.98MetAsn: 1.98 ± 0.086	0.835MetPro: 0.835 ± 0.053	0.543MetGln: 0.543 ± 0.045	0.673MetArg: 0.673 ± 0.04	1.711MetSer: 1.711 ± 0.081	1.126MetThr: 1.126 ± 0.059	1.229MetVal: 1.229 ± 0.056	0.165MetTrp: 0.165 ± 0.026	1.265MetTyr: 1.265 ± 0.066	0.003MetXaa: 0.003 ± 0.003
Asn	3.209AsnAla: 3.209 ± 0.11	0.666AsnCys: 0.666 ± 0.048	4.496AsnAsp: 4.496 ± 0.127	5.725AsnGlu: 5.725 ± 0.128	2.571AsnPhe: 2.571 ± 0.105	3.985AsnGly: 3.985 ± 0.147	0.799AsnHis: 0.799 ± 0.046	8.571AsnIle: 8.571 ± 0.227	8.879AsnLys: 8.879 ± 0.215	6.181AsnLeu: 6.181 ± 0.151	2.177AsnMet: 2.177 ± 0.087	6.815AsnAsn: 6.815 ± 0.226	1.915AsnPro: 1.915 ± 0.093	1.139AsnGln: 1.139 ± 0.069	2.054AsnArg: 2.054 ± 0.084	4.208AsnSer: 4.208 ± 0.132	4.137AsnThr: 4.137 ± 0.151	4.622AsnVal: 4.622 ± 0.141	0.323AsnTrp: 0.323 ± 0.034	3.901AsnTyr: 3.901 ± 0.165	0.0AsnXaa: 0.0 ± 0.0
Pro	0.883ProAla: 0.883 ± 0.054	0.233ProCys: 0.233 ± 0.028	1.229ProAsp: 1.229 ± 0.067	1.456ProGlu: 1.456 ± 0.082	1.2ProPhe: 1.2 ± 0.066	1.261ProGly: 1.261 ± 0.067	0.375ProHis: 0.375 ± 0.048	2.351ProIle: 2.351 ± 0.101	2.028ProLys: 2.028 ± 0.081	2.057ProLeu: 2.057 ± 0.083	0.524ProMet: 0.524 ± 0.042	1.63ProAsn: 1.63 ± 0.075	0.437ProPro: 0.437 ± 0.035	0.417ProGln: 0.417 ± 0.038	0.666ProArg: 0.666 ± 0.051	1.853ProSer: 1.853 ± 0.095	1.669ProThr: 1.669 ± 0.078	1.659ProVal: 1.659 ± 0.068	0.142ProTrp: 0.142 ± 0.025	1.533ProTyr: 1.533 ± 0.077	0.0ProXaa: 0.0 ± 0.0
Gln	0.88GlnAla: 0.88 ± 0.063	0.133GlnCys: 0.133 ± 0.025	0.889GlnAsp: 0.889 ± 0.053	1.071GlnGlu: 1.071 ± 0.065	0.524GlnPhe: 0.524 ± 0.041	0.825GlnGly: 0.825 ± 0.061	0.191GlnHis: 0.191 ± 0.025	1.718GlnIle: 1.718 ± 0.077	1.604GlnLys: 1.604 ± 0.068	1.462GlnLeu: 1.462 ± 0.067	0.56GlnMet: 0.56 ± 0.045	1.126GlnAsn: 1.126 ± 0.071	0.259GlnPro: 0.259 ± 0.028	0.343GlnGln: 0.343 ± 0.029	0.627GlnArg: 0.627 ± 0.045	0.789GlnSer: 0.789 ± 0.05	1.022GlnThr: 1.022 ± 0.066	1.084GlnVal: 1.084 ± 0.062	0.113GlnTrp: 0.113 ± 0.019	0.705GlnTyr: 0.705 ± 0.057	0.0GlnXaa: 0.0 ± 0.0
Arg	1.129ArgAla: 1.129 ± 0.056	0.505ArgCys: 0.505 ± 0.047	1.646ArgAsp: 1.646 ± 0.088	2.154ArgGlu: 2.154 ± 0.098	1.184ArgPhe: 1.184 ± 0.06	1.679ArgGly: 1.679 ± 0.082	0.446ArgHis: 0.446 ± 0.039	3.04ArgIle: 3.04 ± 0.111	3.173ArgLys: 3.173 ± 0.099	2.801ArgLeu: 2.801 ± 0.105	0.88ArgMet: 0.88 ± 0.058	2.031ArgAsn: 2.031 ± 0.097	0.796ArgPro: 0.796 ± 0.058	0.647ArgGln: 0.647 ± 0.056	1.239ArgArg: 1.239 ± 0.064	1.624ArgSer: 1.624 ± 0.081	1.507ArgThr: 1.507 ± 0.069	2.086ArgVal: 2.086 ± 0.091	0.165ArgTrp: 0.165 ± 0.021	1.527ArgTyr: 1.527 ± 0.07	0.003ArgXaa: 0.003 ± 0.003
Ser	2.795SerAla: 2.795 ± 0.115	0.605SerCys: 0.605 ± 0.063	3.852SerAsp: 3.852 ± 0.106	3.985SerGlu: 3.985 ± 0.129	2.93SerPhe: 2.93 ± 0.113	3.885SerGly: 3.885 ± 0.136	0.838SerHis: 0.838 ± 0.059	6.676SerIle: 6.676 ± 0.17	6.857SerLys: 6.857 ± 0.156	6.23SerLeu: 6.23 ± 0.178	1.65SerMet: 1.65 ± 0.09	5.114SerAsn: 5.114 ± 0.17	1.194SerPro: 1.194 ± 0.064	0.961SerGln: 0.961 ± 0.058	1.824SerArg: 1.824 ± 0.081	4.923SerSer: 4.923 ± 0.205	3.37SerThr: 3.37 ± 0.131	4.185SerVal: 4.185 ± 0.125	0.388SerTrp: 0.388 ± 0.035	3.393SerTyr: 3.393 ± 0.126	0.006SerXaa: 0.006 ± 0.004
Thr	1.996ThrAla: 1.996 ± 0.096	0.728ThrCys: 0.728 ± 0.068	3.228ThrAsp: 3.228 ± 0.117	3.04ThrGlu: 3.04 ± 0.125	2.484ThrPhe: 2.484 ± 0.107	3.364ThrGly: 3.364 ± 0.127	0.864ThrHis: 0.864 ± 0.056	5.88ThrIle: 5.88 ± 0.164	5.039ThrLys: 5.039 ± 0.127	5.253ThrLeu: 5.253 ± 0.132	1.071ThrMet: 1.071 ± 0.053	3.878ThrAsn: 3.878 ± 0.141	1.882ThrPro: 1.882 ± 0.088	0.915ThrGln: 0.915 ± 0.057	1.643ThrArg: 1.643 ± 0.084	4.192ThrSer: 4.192 ± 0.2	3.218ThrThr: 3.218 ± 0.143	2.998ThrVal: 2.998 ± 0.13	0.311ThrTrp: 0.311 ± 0.031	2.921ThrTyr: 2.921 ± 0.133	0.003ThrXaa: 0.003 ± 0.003
Val	2.759ValAla: 2.759 ± 0.111	0.864ValCys: 0.864 ± 0.06	4.008ValAsp: 4.008 ± 0.113	3.509ValGlu: 3.509 ± 0.128	2.652ValPhe: 2.652 ± 0.109	3.325ValGly: 3.325 ± 0.116	0.676ValHis: 0.676 ± 0.049	6.89ValIle: 6.89 ± 0.181	5.677ValLys: 5.677 ± 0.165	6.049ValLeu: 6.049 ± 0.153	1.375ValMet: 1.375 ± 0.062	4.38ValAsn: 4.38 ± 0.141	1.659ValPro: 1.659 ± 0.08	0.737ValGln: 0.737 ± 0.043	1.857ValArg: 1.857 ± 0.073	4.787ValSer: 4.787 ± 0.128	4.208ValThr: 4.208 ± 0.174	4.38ValVal: 4.38 ± 0.119	0.314ValTrp: 0.314 ± 0.035	2.837ValTyr: 2.837 ± 0.106	0.0ValXaa: 0.0 ± 0.0
Trp	0.268TrpAla: 0.268 ± 0.03	0.084TrpCys: 0.084 ± 0.016	0.382TrpAsp: 0.382 ± 0.032	0.252TrpGlu: 0.252 ± 0.025	0.23TrpPhe: 0.23 ± 0.032	0.288TrpGly: 0.288 ± 0.03	0.107TrpHis: 0.107 ± 0.019	0.511TrpIle: 0.511 ± 0.046	0.333TrpLys: 0.333 ± 0.028	0.495TrpLeu: 0.495 ± 0.044	0.171TrpMet: 0.171 ± 0.034	0.388TrpAsn: 0.388 ± 0.035	0.113TrpPro: 0.113 ± 0.019	0.123TrpGln: 0.123 ± 0.02	0.158TrpArg: 0.158 ± 0.026	0.281TrpSer: 0.281 ± 0.036	0.256TrpThr: 0.256 ± 0.034	0.265TrpVal: 0.265 ± 0.031	0.061TrpTrp: 0.061 ± 0.014	0.362TrpTyr: 0.362 ± 0.035	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.187TyrAla: 2.187 ± 0.097	0.534TyrCys: 0.534 ± 0.048	3.535TyrAsp: 3.535 ± 0.125	3.668TyrGlu: 3.668 ± 0.135	2.219TyrPhe: 2.219 ± 0.095	2.85TyrGly: 2.85 ± 0.134	0.679TyrHis: 0.679 ± 0.041	5.169TyrIle: 5.169 ± 0.151	5.418TyrLys: 5.418 ± 0.155	4.784TyrLeu: 4.784 ± 0.15	1.132TyrMet: 1.132 ± 0.054	4.143TyrAsn: 4.143 ± 0.151	1.265TyrPro: 1.265 ± 0.077	0.999TyrGln: 0.999 ± 0.068	1.388TyrArg: 1.388 ± 0.068	3.183TyrSer: 3.183 ± 0.106	2.888TyrThr: 2.888 ± 0.117	3.037TyrVal: 3.037 ± 0.098	0.217TyrTrp: 0.217 ± 0.027	2.85TyrTyr: 2.85 ± 0.128	0.003TyrXaa: 0.003 ± 0.003
Xaa	0.0XaaAla: 0.0 ± 0.0	0.003XaaCys: 0.003 ± 0.003	0.003XaaAsp: 0.003 ± 0.003	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.003XaaGly: 0.003 ± 0.003	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.003XaaLys: 0.003 ± 0.003	0.003XaaLeu: 0.003 ± 0.004	0.006XaaMet: 0.006 ± 0.005	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.006XaaArg: 0.006 ± 0.005	0.0XaaSer: 0.0 ± 0.0	0.003XaaThr: 0.003 ± 0.003	0.003XaaVal: 0.003 ± 0.004	0.003XaaTrp: 0.003 ± 0.003	0.003XaaTyr: 0.003 ± 0.004	0.055XaaXaa: 0.055 ± 0.02

Statistics based on 1080 proteins (309167 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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