Pseudomonas phage TL
Average proteome isoelectric point is 6.23
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 65 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|W0XA69|W0XA69_9CAUD Endonuclease OS=Pseudomonas phage TL OX=1406974 GN=gp33 PE=4 SV=1
MM1 pKa = 7.53 ASKK4 pKa = 10.65 KK5 pKa = 10.38 EE6 pKa = 4.02 SLEE9 pKa = 3.98 DD10 pKa = 3.39 QARR13 pKa = 11.84 KK14 pKa = 9.69 EE15 pKa = 3.76 IALEE19 pKa = 4.25 KK20 pKa = 10.13 EE21 pKa = 4.86 FSGSWGGPEE30 pKa = 4.13 IDD32 pKa = 5.3 ADD34 pKa = 4.14 DD35 pKa = 4.73 FPLGSACGLDD45 pKa = 3.54 PEE47 pKa = 4.75 VCEE50 pKa = 4.54 SCTT53 pKa = 3.71
Molecular weight: 5.68 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.303
IPC2_protein 4.139
IPC_protein 3.999
Toseland 3.821
ProMoST 4.126
Dawson 3.961
Bjellqvist 4.113
Wikipedia 3.859
Rodwell 3.834
Grimsley 3.745
Solomon 3.935
Lehninger 3.897
Nozaki 4.088
DTASelect 4.202
Thurlkill 3.872
EMBOSS 3.872
Sillero 4.113
Patrickios 3.63
IPC_peptide 3.935
IPC2_peptide 4.088
IPC2.peptide.svr19 4.017
Protein with the highest isoelectric point:
>tr|W0XA87|W0XA87_9CAUD Uncharacterized protein OS=Pseudomonas phage TL OX=1406974 GN=gp09 PE=4 SV=1
MM1 pKa = 7.57 KK2 pKa = 10.34 SPYY5 pKa = 9.36 EE6 pKa = 4.13 AAHH9 pKa = 5.98 EE10 pKa = 3.94 RR11 pKa = 11.84 ALMVNRR17 pKa = 11.84 LQKK20 pKa = 8.98 LTRR23 pKa = 11.84 MLRR26 pKa = 11.84 VHH28 pKa = 7.34 PDD30 pKa = 3.33 PRR32 pKa = 11.84 WKK34 pKa = 10.29 QEE36 pKa = 3.6 QQEE39 pKa = 4.33 LIKK42 pKa = 10.52 RR43 pKa = 11.84 LKK45 pKa = 10.17 RR46 pKa = 3.55
Molecular weight: 5.71 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.288
IPC2_protein 9.823
IPC_protein 10.818
Toseland 11.155
ProMoST 11.491
Dawson 11.184
Bjellqvist 10.965
Wikipedia 11.462
Rodwell 11.359
Grimsley 11.213
Solomon 11.447
Lehninger 11.389
Nozaki 11.125
DTASelect 10.965
Thurlkill 11.125
EMBOSS 11.579
Sillero 11.14
Patrickios 11.14
IPC_peptide 11.447
IPC2_peptide 9.984
IPC2.peptide.svr19 8.932
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
65
0
65
13295
46
1055
204.5
22.82
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.357 ± 0.659
1.061 ± 0.181
5.927 ± 0.259
7.266 ± 0.404
3.836 ± 0.186
8.379 ± 0.368
2.038 ± 0.229
5.039 ± 0.229
5.799 ± 0.331
8.244 ± 0.223
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.791 ± 0.182
4.22 ± 0.228
4.355 ± 0.235
4.129 ± 0.294
5.844 ± 0.295
5.784 ± 0.268
5.19 ± 0.302
6.882 ± 0.221
1.722 ± 0.148
3.137 ± 0.209
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here