Bacillus phage JBP901

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Herelleviridae; Bastillevirinae; Caeruleovirus; Bacillus virus JBP901

Average proteome isoelectric point is 6.01

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 201 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0E3DEY4|A0A0E3DEY4_9CAUD Putative transcriptional regulator OS=Bacillus phage JBP901 OX=1498212 GN=JBP901_gp157 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 10.04MPWEE6 pKa = 4.75CEE8 pKa = 3.99DD9 pKa = 4.32CGHH12 pKa = 6.98KK13 pKa = 10.38EE14 pKa = 3.65LASPFDD20 pKa = 3.77TFMLCPNCDD29 pKa = 3.22SQNFFHH35 pKa = 7.24GSIIEE40 pKa = 4.57DD41 pKa = 3.67DD42 pKa = 3.85LEE44 pKa = 4.33EE45 pKa = 4.42YY46 pKa = 10.58NVYY49 pKa = 10.71DD50 pKa = 5.6EE51 pKa = 5.56EE52 pKa = 6.53DD53 pKa = 3.44EE54 pKa = 4.41LDD56 pKa = 3.62NVVVFEE62 pKa = 4.29DD63 pKa = 3.69QYY65 pKa = 11.74GEE67 pKa = 4.16KK68 pKa = 9.61LTIVQEE74 pKa = 4.01IDD76 pKa = 3.25DD77 pKa = 5.19DD78 pKa = 5.18GDD80 pKa = 3.96INLLPSDD87 pKa = 3.99RR88 pKa = 11.84NLFFSKK94 pKa = 10.41EE95 pKa = 3.88DD96 pKa = 4.04AIEE99 pKa = 3.86LAKK102 pKa = 10.81AILRR106 pKa = 11.84VCGEE110 pKa = 4.15EE111 pKa = 4.02EE112 pKa = 3.81

Molecular weight: 12.97 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0E3DFB8|A0A0E3DFB8_9CAUD Uncharacterized protein OS=Bacillus phage JBP901 OX=1498212 GN=JBP901_gp200 PE=4 SV=1
MM1 pKa = 6.78VRR3 pKa = 11.84KK4 pKa = 9.58ARR6 pKa = 11.84RR7 pKa = 11.84PRR9 pKa = 11.84LLGSSQTIKK18 pKa = 10.01TGMRR22 pKa = 11.84NLGDD26 pKa = 4.12NLAQDD31 pKa = 4.04VLDD34 pKa = 4.25TGMKK38 pKa = 9.48TIIEE42 pKa = 4.44SKK44 pKa = 9.79PKK46 pKa = 10.26NISAKK51 pKa = 10.31RR52 pKa = 11.84MPKK55 pKa = 9.51YY56 pKa = 10.68LKK58 pKa = 9.59LTEE61 pKa = 4.09EE62 pKa = 4.39RR63 pKa = 11.84LQKK66 pKa = 11.0LEE68 pKa = 4.3VIDD71 pKa = 4.38LKK73 pKa = 11.19PYY75 pKa = 9.1FAKK78 pKa = 10.44SSKK81 pKa = 10.49RR82 pKa = 11.84KK83 pKa = 6.95TKK85 pKa = 10.51KK86 pKa = 10.33DD87 pKa = 3.25GGWYY91 pKa = 8.25LTVPIRR97 pKa = 11.84RR98 pKa = 11.84KK99 pKa = 9.74ARR101 pKa = 11.84GMSRR105 pKa = 11.84RR106 pKa = 11.84MYY108 pKa = 8.73EE109 pKa = 3.45QLRR112 pKa = 11.84AVDD115 pKa = 3.85IGDD118 pKa = 3.49NTKK121 pKa = 8.49QTVVSDD127 pKa = 3.32YY128 pKa = 10.96LYY130 pKa = 10.91DD131 pKa = 3.92RR132 pKa = 11.84RR133 pKa = 11.84RR134 pKa = 11.84QSDD137 pKa = 3.58ASLLNYY143 pKa = 8.06TPKK146 pKa = 10.8SNNINKK152 pKa = 8.89MKK154 pKa = 10.2VGRR157 pKa = 11.84NRR159 pKa = 11.84HH160 pKa = 6.27DD161 pKa = 3.29YY162 pKa = 9.82VAFRR166 pKa = 11.84TVSDD170 pKa = 4.07KK171 pKa = 11.53SPASSWIINRR181 pKa = 11.84DD182 pKa = 3.35KK183 pKa = 11.42VNKK186 pKa = 10.01DD187 pKa = 3.22DD188 pKa = 3.69TSKK191 pKa = 9.88TFVANVNRR199 pKa = 11.84LMKK202 pKa = 9.97WKK204 pKa = 9.06MKK206 pKa = 10.98NGMM209 pKa = 4.0

Molecular weight: 24.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

201

0

201

44905

38

1465

223.4

25.32

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.253 ± 0.204

0.673 ± 0.06

6.251 ± 0.128

7.774 ± 0.297

3.853 ± 0.121

6.643 ± 0.257

1.741 ± 0.095

6.46 ± 0.138

7.783 ± 0.197

7.995 ± 0.185

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.706 ± 0.103

5.425 ± 0.172

3.205 ± 0.172

3.835 ± 0.12

4.376 ± 0.14

5.817 ± 0.176

6.416 ± 0.221

7.211 ± 0.146

1.182 ± 0.079

4.403 ± 0.138
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski