Vulpes vulpes papillomavirus 1
Average proteome isoelectric point is 6.13
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0A7BW90|A0A0A7BW90_9PAPI Replication protein E1 OS=Vulpes vulpes papillomavirus 1 OX=1163709 GN=E1 PE=3 SV=1
MM1 pKa = 7.68 LPRR4 pKa = 11.84 KK5 pKa = 9.11 RR6 pKa = 11.84 AKK8 pKa = 10.22 RR9 pKa = 11.84 DD10 pKa = 3.49 TVDD13 pKa = 3.08 NLYY16 pKa = 10.09 RR17 pKa = 11.84 QCQITGNCPPDD28 pKa = 3.46 VKK30 pKa = 11.11 NKK32 pKa = 10.12 VEE34 pKa = 4.18 GTTLADD40 pKa = 3.12 ILSKK44 pKa = 10.38 IFSSIIYY51 pKa = 9.31 TGGLGIGTGKK61 pKa = 8.8 GTGGTGGYY69 pKa = 9.4 RR70 pKa = 11.84 PLGGTTGSRR79 pKa = 11.84 GPGTVSRR86 pKa = 11.84 PNIPVDD92 pKa = 4.07 PIGPADD98 pKa = 3.96 ILPINPVEE106 pKa = 4.31 PNASSIVPLNEE117 pKa = 3.96 GDD119 pKa = 3.41 PGIIIEE125 pKa = 4.79 PPGTVNGADD134 pKa = 3.38 VEE136 pKa = 4.48 LSVVTSIDD144 pKa = 3.47 PVSDD148 pKa = 3.41 VSNASIPAPTGAGSDD163 pKa = 3.38 GDD165 pKa = 3.9 AAIIDD170 pKa = 4.18 VQTSTRR176 pKa = 11.84 TTNAVRR182 pKa = 11.84 STQRR186 pKa = 11.84 FNNPVFEE193 pKa = 4.48 TVAIVHH199 pKa = 6.5 RR200 pKa = 11.84 SFGEE204 pKa = 3.83 SSEE207 pKa = 3.99 NLNVVVDD214 pKa = 3.98 PTILQDD220 pKa = 3.54 TVIGGDD226 pKa = 2.9 EE227 pKa = 4.45 GEE229 pKa = 4.65 FIEE232 pKa = 5.87 LDD234 pKa = 3.05 ILGRR238 pKa = 11.84 PSQFEE243 pKa = 3.99 IVEE246 pKa = 4.21 GEE248 pKa = 4.31 GPKK251 pKa = 10.0 TSTPIEE257 pKa = 4.1 RR258 pKa = 11.84 LAGPFKK264 pKa = 10.65 RR265 pKa = 11.84 ARR267 pKa = 11.84 EE268 pKa = 4.02 LYY270 pKa = 9.81 NRR272 pKa = 11.84 RR273 pKa = 11.84 IKK275 pKa = 10.19 QIPTQNPLFLGRR287 pKa = 11.84 AAEE290 pKa = 3.88 AVEE293 pKa = 5.0 FGFEE297 pKa = 3.82 NPAYY301 pKa = 9.66 EE302 pKa = 5.23 DD303 pKa = 4.49 DD304 pKa = 3.79 VTLTFEE310 pKa = 4.96 RR311 pKa = 11.84 DD312 pKa = 3.37 LDD314 pKa = 3.89 EE315 pKa = 5.1 LAAAPVAAPDD325 pKa = 4.17 PDD327 pKa = 3.58 FADD330 pKa = 3.07 IVKK333 pKa = 10.59 LSRR336 pKa = 11.84 PRR338 pKa = 11.84 LSEE341 pKa = 3.97 PEE343 pKa = 4.02 PGRR346 pKa = 11.84 VRR348 pKa = 11.84 YY349 pKa = 9.75 SRR351 pKa = 11.84 LGRR354 pKa = 11.84 RR355 pKa = 11.84 GTMTLRR361 pKa = 11.84 SGVQIGEE368 pKa = 4.05 EE369 pKa = 3.92 VHH371 pKa = 7.03 FYY373 pKa = 11.04 RR374 pKa = 11.84 DD375 pKa = 3.26 LSTIDD380 pKa = 3.55 DD381 pKa = 3.9 AQGIEE386 pKa = 4.3 LSVLGHH392 pKa = 5.33 QSGEE396 pKa = 4.07 EE397 pKa = 4.1 VIIDD401 pKa = 3.99 PLSEE405 pKa = 4.12 STVIDD410 pKa = 3.66 AEE412 pKa = 4.58 NISEE416 pKa = 4.14 PQFDD420 pKa = 3.86 YY421 pKa = 11.5 DD422 pKa = 3.86 EE423 pKa = 4.91 YY424 pKa = 11.46 EE425 pKa = 4.14 HH426 pKa = 7.12 LLLDD430 pKa = 4.13 EE431 pKa = 4.78 LSEE434 pKa = 4.61 DD435 pKa = 3.91 FSNSHH440 pKa = 6.54 LVLNSTGRR448 pKa = 11.84 NSSLTMPSMPPGTPLKK464 pKa = 10.61 IFIDD468 pKa = 4.46 DD469 pKa = 3.82 YY470 pKa = 11.47 GSLIVAHH477 pKa = 6.51 SSSTEE482 pKa = 3.73 SDD484 pKa = 4.7 DD485 pKa = 3.62 IPSTIWEE492 pKa = 4.23 NLQPVIVVSPFEE504 pKa = 4.07 SFDD507 pKa = 4.0 YY508 pKa = 10.75 NLHH511 pKa = 6.08 PSLRR515 pKa = 11.84 RR516 pKa = 11.84 KK517 pKa = 8.88 KK518 pKa = 10.06 RR519 pKa = 11.84 KK520 pKa = 9.24 RR521 pKa = 11.84 PYY523 pKa = 10.36 FFF525 pKa = 5.75
Molecular weight: 56.94 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.669
IPC2_protein 4.571
IPC_protein 4.533
Toseland 4.38
ProMoST 4.647
Dawson 4.482
Bjellqvist 4.635
Wikipedia 4.355
Rodwell 4.38
Grimsley 4.291
Solomon 4.482
Lehninger 4.444
Nozaki 4.596
DTASelect 4.762
Thurlkill 4.38
EMBOSS 4.38
Sillero 4.647
Patrickios 4.088
IPC_peptide 4.495
IPC2_peptide 4.647
IPC2.peptide.svr19 4.582
Protein with the highest isoelectric point:
>tr|A0A0A7BWG9|A0A0A7BWG9_9PAPI Regulatory protein E2 OS=Vulpes vulpes papillomavirus 1 OX=1163709 GN=E2 PE=3 SV=1
MM1 pKa = 6.59 EE2 pKa = 4.56 TLRR5 pKa = 11.84 EE6 pKa = 3.97 RR7 pKa = 11.84 FDD9 pKa = 3.9 ALQEE13 pKa = 4.04 QLLTLYY19 pKa = 10.08 EE20 pKa = 4.08 QEE22 pKa = 4.45 NNDD25 pKa = 4.37 LEE27 pKa = 4.55 SQLLHH32 pKa = 6.44 WKK34 pKa = 9.31 LQRR37 pKa = 11.84 KK38 pKa = 8.55 EE39 pKa = 4.06 SALMYY44 pKa = 9.03 YY45 pKa = 10.1 ARR47 pKa = 11.84 KK48 pKa = 10.0 NGLASLGMQRR58 pKa = 11.84 LPSLQVSEE66 pKa = 4.43 QQAKK70 pKa = 8.76 QAILMTLYY78 pKa = 10.9 LEE80 pKa = 4.79 SLSKK84 pKa = 9.62 TPYY87 pKa = 10.61 AKK89 pKa = 10.35 EE90 pKa = 3.4 HH91 pKa = 5.88 WTLQDD96 pKa = 2.91 TSYY99 pKa = 10.92 EE100 pKa = 4.08 RR101 pKa = 11.84 FMGQPPYY108 pKa = 10.38 CFKK111 pKa = 10.87 KK112 pKa = 10.75 GPFTVDD118 pKa = 2.65 VVYY121 pKa = 11.04 DD122 pKa = 3.65 KK123 pKa = 11.34 DD124 pKa = 4.09 SNNMFSYY131 pKa = 10.54 TNYY134 pKa = 10.53 NFIYY138 pKa = 10.48 YY139 pKa = 9.66 QDD141 pKa = 4.09 EE142 pKa = 4.32 DD143 pKa = 4.57 EE144 pKa = 4.69 NWHH147 pKa = 5.59 KK148 pKa = 9.81 TSGEE152 pKa = 3.83 VSYY155 pKa = 11.18 EE156 pKa = 3.48 GLYY159 pKa = 8.53 YY160 pKa = 10.21 TDD162 pKa = 3.29 YY163 pKa = 10.75 KK164 pKa = 10.57 GRR166 pKa = 11.84 SVYY169 pKa = 10.0 FVYY172 pKa = 10.64 FEE174 pKa = 5.24 KK175 pKa = 10.78 DD176 pKa = 2.97 AQLYY180 pKa = 8.39 SQYY183 pKa = 9.82 GTWEE187 pKa = 3.9 VHH189 pKa = 6.57 FKK191 pKa = 10.72 NQTISASVVSTSTGTGSGAYY211 pKa = 9.26 DD212 pKa = 4.1 SPDD215 pKa = 3.02 SSPPGRR221 pKa = 11.84 RR222 pKa = 11.84 STGRR226 pKa = 11.84 PRR228 pKa = 11.84 VSEE231 pKa = 3.84 ARR233 pKa = 11.84 QPANTSPTAYY243 pKa = 9.64 PEE245 pKa = 4.36 ASEE248 pKa = 4.0 KK249 pKa = 9.53 TALRR253 pKa = 11.84 KK254 pKa = 9.84 RR255 pKa = 11.84 PGPGPGQTSPSPSAGAPKK273 pKa = 9.91 RR274 pKa = 11.84 RR275 pKa = 11.84 RR276 pKa = 11.84 RR277 pKa = 11.84 GEE279 pKa = 3.81 RR280 pKa = 11.84 EE281 pKa = 3.55 QVPRR285 pKa = 11.84 QLRR288 pKa = 11.84 STPSVSSGTPWPSPGQVGRR307 pKa = 11.84 RR308 pKa = 11.84 SRR310 pKa = 11.84 LPPRR314 pKa = 11.84 HH315 pKa = 5.56 NSSRR319 pKa = 11.84 LRR321 pKa = 11.84 RR322 pKa = 11.84 LQAEE326 pKa = 3.99 AWDD329 pKa = 4.1 PPVILLKK336 pKa = 11.05 GKK338 pKa = 10.35 ANNLKK343 pKa = 9.99 CWRR346 pKa = 11.84 NRR348 pKa = 11.84 VKK350 pKa = 10.51 QRR352 pKa = 11.84 NLDD355 pKa = 3.67 FKK357 pKa = 10.95 IVFSSLFNWIGLSHH371 pKa = 6.55 TEE373 pKa = 3.98 HH374 pKa = 6.64 GKK376 pKa = 9.56 HH377 pKa = 6.23 RR378 pKa = 11.84 MLLAFDD384 pKa = 4.21 GEE386 pKa = 4.33 KK387 pKa = 10.39 EE388 pKa = 3.8 RR389 pKa = 11.84 RR390 pKa = 11.84 LFLDD394 pKa = 3.48 TVKK397 pKa = 10.63 LPKK400 pKa = 10.51 GVTYY404 pKa = 10.95 SLGNLDD410 pKa = 3.96 SLL412 pKa = 4.83
Molecular weight: 47.28 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.846
IPC2_protein 9.107
IPC_protein 9.092
Toseland 9.531
ProMoST 9.399
Dawson 9.853
Bjellqvist 9.589
Wikipedia 10.072
Rodwell 10.043
Grimsley 9.955
Solomon 9.867
Lehninger 9.823
Nozaki 9.545
DTASelect 9.575
Thurlkill 9.663
EMBOSS 9.97
Sillero 9.765
Patrickios 4.787
IPC_peptide 9.867
IPC2_peptide 8.2
IPC2.peptide.svr19 8.007
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
2303
96
623
383.8
42.98
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.037 ± 0.387
2.084 ± 0.739
6.036 ± 0.548
6.34 ± 0.335
4.516 ± 0.354
6.687 ± 0.684
1.693 ± 0.237
4.342 ± 0.864
5.211 ± 0.762
9.857 ± 0.835
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.563 ± 0.295
4.907 ± 0.562
6.426 ± 0.743
3.995 ± 0.503
5.775 ± 0.844
8.641 ± 0.61
6.296 ± 0.381
6.166 ± 0.45
1.259 ± 0.308
3.17 ± 0.536
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here