Pseudomonas phage O4

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Zobellviridae; Paundecimvirus; unclassified Paundecimvirus

Average proteome isoelectric point is 6.29

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 76 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A127KN61|A0A127KN61_9CAUD Putative ATP-grasp protein OS=Pseudomonas phage O4 OX=1784982 GN=O4_52 PE=4 SV=1
MM1 pKa = 7.45VIYY4 pKa = 9.55VWPDD8 pKa = 3.46GYY10 pKa = 10.25WLYY13 pKa = 11.22EE14 pKa = 3.88WAVEE18 pKa = 4.46DD19 pKa = 3.86NASLARR25 pKa = 11.84LASPHH30 pKa = 6.46KK31 pKa = 10.82KK32 pKa = 9.22IDD34 pKa = 3.73LDD36 pKa = 3.52NHH38 pKa = 6.79LDD40 pKa = 3.51YY41 pKa = 11.39DD42 pKa = 4.07LTVDD46 pKa = 5.08EE47 pKa = 5.29INACIEE53 pKa = 4.23ALDD56 pKa = 3.93EE57 pKa = 4.3

Molecular weight: 6.65 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A140IES0|A0A140IES0_9CAUD Putative amidotransferase OS=Pseudomonas phage O4 OX=1784982 GN=O4_58 PE=4 SV=1
MM1 pKa = 7.77KK2 pKa = 10.26LPKK5 pKa = 9.8YY6 pKa = 10.7VYY8 pKa = 10.31LIYY11 pKa = 11.26VNGLYY16 pKa = 10.49YY17 pKa = 10.5SSRR20 pKa = 11.84ANRR23 pKa = 11.84EE24 pKa = 3.57AARR27 pKa = 11.84DD28 pKa = 3.66SARR31 pKa = 11.84SAKK34 pKa = 10.26KK35 pKa = 9.77FLRR38 pKa = 11.84TKK40 pKa = 10.74NVIIYY45 pKa = 8.53QVSVKK50 pKa = 10.88DD51 pKa = 3.61EE52 pKa = 4.02LVKK55 pKa = 10.7VVRR58 pKa = 4.55

Molecular weight: 6.81 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

76

0

76

15840

40

1554

208.4

23.52

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.15 ± 0.423

1.124 ± 0.145

6.332 ± 0.201

7.109 ± 0.292

4.021 ± 0.218

7.014 ± 0.321

2.001 ± 0.167

5.486 ± 0.22

7.008 ± 0.374

7.727 ± 0.256

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.645 ± 0.148

5.145 ± 0.208

3.693 ± 0.173

3.902 ± 0.398

5.215 ± 0.207

5.455 ± 0.301

5.29 ± 0.297

7.039 ± 0.233

1.749 ± 0.136

3.895 ± 0.163
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski