Pseudomonas phage UNO-SLW2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Studiervirinae; Pifdecavirus; unclassified Pifdecavirus

Average proteome isoelectric point is 6.65

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 48 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1B2AN73|A0A1B2AN73_9CAUD Uncharacterized protein OS=Pseudomonas phage UNO-SLW2 OX=1874529 GN=UNOSLW2_0195 PE=4 SV=1
MM1 pKa = 6.83STVTIPEE8 pKa = 3.99KK9 pKa = 10.72EE10 pKa = 4.0YY11 pKa = 11.45DD12 pKa = 3.74EE13 pKa = 5.51LLADD17 pKa = 4.06SNHH20 pKa = 5.6LRR22 pKa = 11.84MLINRR27 pKa = 11.84GVDD30 pKa = 2.74NWDD33 pKa = 3.76GYY35 pKa = 8.78VSRR38 pKa = 11.84HH39 pKa = 5.8HH40 pKa = 6.59EE41 pKa = 4.1EE42 pKa = 4.11CDD44 pKa = 3.33EE45 pKa = 4.7CGLDD49 pKa = 5.85EE50 pKa = 6.48DD51 pKa = 5.81DD52 pKa = 4.35CTCGGGEE59 pKa = 3.92

Molecular weight: 6.67 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1P8EDN4|A0A1P8EDN4_9CAUD Putative Rz1-like protein OS=Pseudomonas phage UNO-SLW2 OX=1874529 GN=UNOSLW2_0227 PE=4 SV=1
MM1 pKa = 7.76ASTRR5 pKa = 11.84NTRR8 pKa = 11.84GSLSCTKK15 pKa = 10.02PKK17 pKa = 10.43PNALRR22 pKa = 11.84RR23 pKa = 11.84PVMKK27 pKa = 10.7SNPNSMASPSATPKK41 pKa = 7.33THH43 pKa = 6.96KK44 pKa = 10.38GLKK47 pKa = 10.06ALLLGLWLSFVIAVSGCQSNLPTPSEE73 pKa = 4.09KK74 pKa = 10.04PSRR77 pKa = 11.84HH78 pKa = 5.62TIEE81 pKa = 4.32ASLMVEE87 pKa = 3.9PNYY90 pKa = 8.19TQRR93 pKa = 11.84LLKK96 pKa = 10.54LLSEE100 pKa = 4.62

Molecular weight: 10.87 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

48

0

48

12220

52

1330

254.6

28.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.27 ± 0.598

0.835 ± 0.133

6.293 ± 0.224

6.882 ± 0.387

3.552 ± 0.192

8.232 ± 0.328

2.242 ± 0.194

4.517 ± 0.165

5.982 ± 0.398

8.347 ± 0.346

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.946 ± 0.204

3.887 ± 0.202

3.92 ± 0.214

4.067 ± 0.327

5.982 ± 0.175

5.319 ± 0.227

5.679 ± 0.257

6.678 ± 0.33

1.465 ± 0.148

2.905 ± 0.237
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski