Trema orientale (Charcoal tree) (Celtis orientalis)

Taxonomy: cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliopsida; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; fabids;

Average proteome isoelectric point is 6.89

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 35801 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2P5FUE3|A0A2P5FUE3_TREOI E3 ubiquitin-protein ligase RHF2A OS=Trema orientale OX=63057 GN=TorRG33x02_027070 PE=4 SV=1
MM1 pKa = 7.19TLKK4 pKa = 10.98LNDD7 pKa = 3.82EE8 pKa = 4.89ANGNWWLEE16 pKa = 3.61IGEE19 pKa = 4.73NDD21 pKa = 3.64TQIGFWPQRR30 pKa = 11.84IFSGGLNDD38 pKa = 4.7LATYY42 pKa = 10.46LDD44 pKa = 3.98WGGEE48 pKa = 4.17AYY50 pKa = 10.57SPPNEE55 pKa = 4.29SGLPQMGSGFILSGSVYY72 pKa = 10.16EE73 pKa = 6.27DD74 pKa = 3.76SFCEE78 pKa = 3.98QITTINEE85 pKa = 3.48AHH87 pKa = 7.04DD88 pKa = 4.04PVDD91 pKa = 3.94AGDD94 pKa = 3.79TEE96 pKa = 4.9VIGNEE101 pKa = 4.09NVTLPYY107 pKa = 10.36GVRR110 pKa = 11.84DD111 pKa = 3.86YY112 pKa = 11.23PDD114 pKa = 3.1TGGDD118 pKa = 3.45LRR120 pKa = 11.84HH121 pKa = 5.97LVSFGGPGSS130 pKa = 3.45

Molecular weight: 14.09 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2P5CF02|A0A2P5CF02_TREOI Uncharacterized protein OS=Trema orientale OX=63057 GN=TorRG33x02_287880 PE=4 SV=1
HHH2 pKa = 7.6RR3 pKa = 11.84RR4 pKa = 11.84GAVVSGTGLRR14 pKa = 11.84TFVGSLRR21 pKa = 11.84FTRR24 pKa = 11.84VGTSAVGRR32 pKa = 11.84SGATARR38 pKa = 11.84LVRR41 pKa = 11.84KKK43 pKa = 9.82SSLWASI

Molecular weight: 5.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

35798

3

35801

11250880

24

5546

314.3

35.08

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.6 ± 0.014

1.834 ± 0.008

5.231 ± 0.011

6.424 ± 0.016

4.308 ± 0.01

6.58 ± 0.014

2.403 ± 0.007

5.271 ± 0.011

5.953 ± 0.013

9.931 ± 0.02

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.308 ± 0.006

4.425 ± 0.01

4.946 ± 0.012

3.533 ± 0.011

5.51 ± 0.011

9.123 ± 0.016

4.909 ± 0.008

6.613 ± 0.01

1.321 ± 0.006

2.764 ± 0.009
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski