Papaya meleira virus
Average proteome isoelectric point is 8.43
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0P0QL14|A0A0P0QL14_9VIRU RNA-directed RNA polymerase OS=Papaya meleira virus OX=1497848 GN=pol PE=3 SV=1
MM1 pKa = 7.5 AASQGRR7 pKa = 11.84 YY8 pKa = 9.13 CEE10 pKa = 3.99 EE11 pKa = 3.63 RR12 pKa = 11.84 FNLFKK17 pKa = 10.66 EE18 pKa = 4.32 YY19 pKa = 10.51 TSLNEE24 pKa = 4.83 FPDD27 pKa = 3.81 LNLKK31 pKa = 10.14 VEE33 pKa = 4.26 EE34 pKa = 4.25 TFKK37 pKa = 11.44 NFVFKK42 pKa = 10.9 LSEE45 pKa = 4.13 IEE47 pKa = 4.2 GLPLLSHH54 pKa = 7.0 CNLLIDD60 pKa = 4.87 IKK62 pKa = 11.19 GFLDD66 pKa = 3.87 KK67 pKa = 10.96 FPLIHH72 pKa = 6.88 YY73 pKa = 8.03 DD74 pKa = 3.6 YY75 pKa = 11.2 NNSNFRR81 pKa = 11.84 NIVTWIFIEE90 pKa = 3.97 PLPRR94 pKa = 11.84 EE95 pKa = 4.18 IYY97 pKa = 10.27 SYY99 pKa = 11.55 LPVKK103 pKa = 10.39 LFNTINIFILKK114 pKa = 9.84 DD115 pKa = 3.57 LEE117 pKa = 4.6 KK118 pKa = 10.23 EE119 pKa = 3.94 LPKK122 pKa = 10.57 INFISNLILNKK133 pKa = 10.4 NNLEE137 pKa = 3.78 ITNNLFFKK145 pKa = 10.73 PLGRR149 pKa = 11.84 LGGKK153 pKa = 6.24 THH155 pKa = 7.39 LLIGDD160 pKa = 4.21 FLKK163 pKa = 11.05 LFNFSFSDD171 pKa = 3.12 IGRR174 pKa = 11.84 EE175 pKa = 3.52 EE176 pKa = 4.49 DD177 pKa = 3.07 KK178 pKa = 11.15 GKK180 pKa = 10.72 KK181 pKa = 8.79 VVHH184 pKa = 6.02 ILINKK189 pKa = 8.38 NRR191 pKa = 11.84 VNNNIGIYY199 pKa = 10.45 DD200 pKa = 4.07 FNNLIKK206 pKa = 10.74 LNKK209 pKa = 9.03 DD210 pKa = 3.53 TILSNDD216 pKa = 3.51 IIYY219 pKa = 9.22 TLISVLLYY227 pKa = 10.26 QLEE230 pKa = 4.48 LGCEE234 pKa = 4.13 EE235 pKa = 4.35 SWNSLNVAGLLISPITSPLGFICTLFFISSNITKK269 pKa = 8.93 LTILEE274 pKa = 3.99 ASKK277 pKa = 10.34 ICKK280 pKa = 9.85 KK281 pKa = 10.16 FQNTVRR287 pKa = 11.84 IGGRR291 pKa = 11.84 LPIFNIPITDD301 pKa = 3.67 DD302 pKa = 3.07 NKK304 pKa = 10.87 YY305 pKa = 10.7 FLDD308 pKa = 4.17 GQKK311 pKa = 10.86 GILSIEE317 pKa = 4.86 GIRR320 pKa = 11.84 MLYY323 pKa = 10.63 GIDD326 pKa = 3.64 SLLGPSEE333 pKa = 4.4 FYY335 pKa = 10.67 KK336 pKa = 10.65 YY337 pKa = 10.9 DD338 pKa = 4.9 PISEE342 pKa = 4.36 IITRR346 pKa = 11.84 QVHH349 pKa = 6.1 PNLEE353 pKa = 3.84 LSLYY357 pKa = 10.5 KK358 pKa = 10.7 GGSRR362 pKa = 11.84 SNKK365 pKa = 9.39 AYY367 pKa = 10.49 SSTIEE372 pKa = 3.9 AVNKK376 pKa = 9.7 RR377 pKa = 11.84 EE378 pKa = 3.94 EE379 pKa = 4.38 IIRR382 pKa = 11.84 QDD384 pKa = 3.26 MYY386 pKa = 11.29 NSLFFSVVKK395 pKa = 10.47 KK396 pKa = 8.9 LHH398 pKa = 6.52 RR399 pKa = 11.84 PFKK402 pKa = 10.25 TFRR405 pKa = 11.84 EE406 pKa = 4.09 WFNIKK411 pKa = 10.21 FSWSASGGCPGAKK424 pKa = 9.85 VFWSEE429 pKa = 4.1 SEE431 pKa = 4.03 KK432 pKa = 10.68 IRR434 pKa = 11.84 VNKK437 pKa = 10.19 RR438 pKa = 11.84 GALLNVKK445 pKa = 9.83 VDD447 pKa = 4.15 DD448 pKa = 3.95 VVKK451 pKa = 10.83 AINEE455 pKa = 4.41 TYY457 pKa = 10.56 NNPVHH462 pKa = 6.08 YY463 pKa = 10.45 SKK465 pKa = 11.04 AARR468 pKa = 11.84 KK469 pKa = 8.91 YY470 pKa = 8.46 EE471 pKa = 3.9 KK472 pKa = 10.67 GKK474 pKa = 10.12 NRR476 pKa = 11.84 VIWNTTLLMYY486 pKa = 10.3 LAQSYY491 pKa = 10.61 LLYY494 pKa = 9.98 TFEE497 pKa = 6.42 SILDD501 pKa = 3.93 PLQHH505 pKa = 6.92 DD506 pKa = 4.68 FSFEE510 pKa = 4.08 LKK512 pKa = 10.5 QISSWNSSTANADD525 pKa = 2.91 MRR527 pKa = 11.84 FISNQEE533 pKa = 3.75 RR534 pKa = 11.84 LRR536 pKa = 11.84 SLKK539 pKa = 10.8 DD540 pKa = 3.19 NSGLMFDD547 pKa = 4.57 YY548 pKa = 11.23 SDD550 pKa = 4.25 FNINHH555 pKa = 6.69 SIVVQNKK562 pKa = 9.75 LYY564 pKa = 10.86 DD565 pKa = 3.56 VLSQVLFNKK574 pKa = 9.69 MKK576 pKa = 10.6 VNQEE580 pKa = 3.72 NYY582 pKa = 9.79 FQVLKK587 pKa = 10.31 DD588 pKa = 3.5 IKK590 pKa = 10.39 WITEE594 pKa = 3.93 YY595 pKa = 10.63 ILRR598 pKa = 11.84 AKK600 pKa = 8.99 TFSVLDD606 pKa = 3.73 AGVTDD611 pKa = 4.26 NYY613 pKa = 10.2 VSQVMRR619 pKa = 11.84 SLEE622 pKa = 4.0 SGEE625 pKa = 4.49 RR626 pKa = 11.84 GTSFINTFLSNCYY639 pKa = 9.5 IRR641 pKa = 11.84 NLNTNSRR648 pKa = 11.84 SLFGRR653 pKa = 11.84 QLLINNLWLQGDD665 pKa = 4.34 DD666 pKa = 3.49 VFAMTKK672 pKa = 10.1 NLEE675 pKa = 4.57 DD676 pKa = 3.49 GCLAVNLFNCLGYY689 pKa = 10.63 AGQPSKK695 pKa = 10.84 IGLSLYY701 pKa = 11.07 NNGEE705 pKa = 4.31 FLRR708 pKa = 11.84 LSYY711 pKa = 10.91 DD712 pKa = 3.48 PSCEE716 pKa = 3.97 IIGGYY721 pKa = 8.69 PIRR724 pKa = 11.84 STSGLISGEE733 pKa = 3.94 FFKK736 pKa = 11.06 EE737 pKa = 4.1 SIYY740 pKa = 11.28 DD741 pKa = 3.59 PDD743 pKa = 3.79 TRR745 pKa = 11.84 AGCYY749 pKa = 8.85 LSSVIRR755 pKa = 11.84 CNVRR759 pKa = 11.84 GAIIPNNLIYY769 pKa = 10.28 ILCQRR774 pKa = 11.84 NCRR777 pKa = 11.84 LIYY780 pKa = 8.85 TKK782 pKa = 10.42 PNSKK786 pKa = 10.3 EE787 pKa = 4.01 KK788 pKa = 10.36 IVMIGDD794 pKa = 3.65 PVLALIPSILGGFGINSILDD814 pKa = 3.2 IGMKK818 pKa = 9.84 HH819 pKa = 5.17 QVPGTDD825 pKa = 2.82 QLLNRR830 pKa = 11.84 LQLSPKK836 pKa = 8.72 MISGGWFSSKK846 pKa = 9.87 FKK848 pKa = 10.83 NLNPPKK854 pKa = 10.17 DD855 pKa = 3.7 SLPIGEE861 pKa = 5.07 LFWIRR866 pKa = 11.84 RR867 pKa = 11.84 VSNNSNNNFNSYY879 pKa = 10.31 YY880 pKa = 10.6 VINTTKK886 pKa = 10.56 SNPILRR892 pKa = 11.84 FDD894 pKa = 5.2 NIPDD898 pKa = 3.4 ILGDD902 pKa = 3.5 KK903 pKa = 10.27 SIPDD907 pKa = 3.69 FLSAYY912 pKa = 6.97 TICNKK917 pKa = 10.35 SLTKK921 pKa = 10.47 EE922 pKa = 3.47 ITQNIVKK929 pKa = 9.87 SSVMGALPLDD939 pKa = 4.38 LLSKK943 pKa = 10.76 SLTKK947 pKa = 10.79 YY948 pKa = 11.0 GEE950 pKa = 4.19 DD951 pKa = 3.24 QLEE954 pKa = 4.03 YY955 pKa = 10.17 RR956 pKa = 11.84 SKK958 pKa = 11.54 CNVHH962 pKa = 4.24 WVKK965 pKa = 9.72 YY966 pKa = 7.49 TSNFGSLNIKK976 pKa = 10.32 LSFISHH982 pKa = 6.69 YY983 pKa = 9.76 ISNFIKK989 pKa = 10.49 FLISDD994 pKa = 4.65 EE995 pKa = 4.46 IKK997 pKa = 9.49 TSHH1000 pKa = 7.15 KK1001 pKa = 10.17 IDD1003 pKa = 2.97 ISVNLNYY1010 pKa = 10.89 GILPTLFIEE1019 pKa = 4.83 YY1020 pKa = 10.32 GFSLQEE1026 pKa = 3.71 NFEE1029 pKa = 4.19 RR1030 pKa = 11.84 VIDD1033 pKa = 3.99 EE1034 pKa = 4.89 LNTKK1038 pKa = 9.68 EE1039 pKa = 4.25 LLEE1042 pKa = 5.1 LIASKK1047 pKa = 7.55 QTKK1050 pKa = 9.48 KK1051 pKa = 10.86 FSITSFFKK1059 pKa = 11.05 NILILNKK1066 pKa = 10.29 YY1067 pKa = 6.55 NTKK1070 pKa = 10.68 LYY1072 pKa = 10.63 LKK1074 pKa = 8.9 WIKK1077 pKa = 10.67 GKK1079 pKa = 10.03 ILLFPPVFSLIDD1091 pKa = 3.5 SDD1093 pKa = 5.78 IINIWRR1099 pKa = 11.84 GWSLFSVEE1107 pKa = 5.05 KK1108 pKa = 10.35 MILHH1112 pKa = 6.1 GVNLRR1117 pKa = 11.84 EE1118 pKa = 4.5 DD1119 pKa = 4.99 DD1120 pKa = 3.36 IPYY1123 pKa = 10.16 IILEE1127 pKa = 4.27 LEE1129 pKa = 4.16 LLIEE1133 pKa = 4.29 NYY1135 pKa = 9.98 IKK1137 pKa = 10.82 DD1138 pKa = 3.73 SLEE1141 pKa = 3.91 SLNKK1145 pKa = 9.36 INKK1148 pKa = 8.62 FYY1150 pKa = 11.56 SMQTKK1155 pKa = 10.07 AA1156 pKa = 4.14
Molecular weight: 132.88 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.145
IPC2_protein 8.214
IPC_protein 8.039
Toseland 8.448
ProMoST 8.609
Dawson 8.96
Bjellqvist 8.946
Wikipedia 9.077
Rodwell 9.107
Grimsley 8.799
Solomon 9.004
Lehninger 8.975
Nozaki 9.048
DTASelect 8.814
Thurlkill 8.902
EMBOSS 9.107
Sillero 9.107
Patrickios 4.38
IPC_peptide 8.99
IPC2_peptide 7.6
IPC2.peptide.svr19 7.676
Protein with the highest isoelectric point:
>tr|A0A0P0QKV0|A0A0P0QKV0_9VIRU Putative structural protein OS=Papaya meleira virus OX=1497848 GN=gag PE=4 SV=1
MM1 pKa = 7.41 VEE3 pKa = 4.51 RR4 pKa = 11.84 LTGMTNGFCHH14 pKa = 6.89 RR15 pKa = 11.84 LNSPALNRR23 pKa = 11.84 GRR25 pKa = 11.84 PILLSNCYY33 pKa = 10.09 LPLSNFSSNRR43 pKa = 11.84 SKK45 pKa = 11.13 DD46 pKa = 2.81 IGRR49 pKa = 11.84 ATRR52 pKa = 11.84 LAFEE56 pKa = 5.12 LPDD59 pKa = 3.86 LLSPSFLISLTLHH72 pKa = 6.37 FEE74 pKa = 3.67 AHH76 pKa = 6.94 FISFVYY82 pKa = 10.15 II83 pKa = 3.73
Molecular weight: 9.4 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.911
IPC2_protein 8.595
IPC_protein 9.077
Toseland 8.946
ProMoST 8.975
Dawson 9.224
Bjellqvist 9.385
Wikipedia 9.326
Rodwell 9.224
Grimsley 8.858
Solomon 9.604
Lehninger 9.575
Nozaki 9.502
DTASelect 9.18
Thurlkill 9.194
EMBOSS 9.385
Sillero 9.458
Patrickios 4.902
IPC_peptide 9.575
IPC2_peptide 8.946
IPC2.peptide.svr19 7.911
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
2835
83
1596
945.0
107.76
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
3.633 ± 0.8
1.199 ± 0.317
4.656 ± 0.541
5.15 ± 0.368
5.115 ± 1.073
5.15 ± 0.144
2.046 ± 0.597
8.536 ± 1.207
6.772 ± 1.414
10.265 ± 2.09
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.623 ± 0.258
8.289 ± 0.517
5.256 ± 1.069
3.069 ± 0.882
4.656 ± 0.997
8.924 ± 0.759
4.974 ± 0.544
5.397 ± 0.994
1.199 ± 0.287
4.092 ± 0.414
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here