Tortoise microvirus 55
Average proteome isoelectric point is 6.07
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4P8W7G9|A0A4P8W7G9_9VIRU Uncharacterized protein OS=Tortoise microvirus 55 OX=2583160 PE=4 SV=1
MM1 pKa = 7.5 KK2 pKa = 10.7 NNVYY6 pKa = 8.78 TLYY9 pKa = 9.29 NTLSSRR15 pKa = 11.84 YY16 pKa = 8.69 GEE18 pKa = 4.25 VMSFPSDD25 pKa = 2.84 AFAVQRR31 pKa = 11.84 IRR33 pKa = 11.84 EE34 pKa = 4.12 AMKK37 pKa = 10.26 PEE39 pKa = 3.71 MLAEE43 pKa = 4.27 IEE45 pKa = 4.2 LCNVGFIDD53 pKa = 3.77 IDD55 pKa = 3.61 TGIVEE60 pKa = 4.03 AHH62 pKa = 5.87 APVRR66 pKa = 11.84 ISLTSPEE73 pKa = 4.41 SPVPIDD79 pKa = 3.71 NLEE82 pKa = 3.95 KK83 pKa = 10.87
Molecular weight: 9.27 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.681
IPC2_protein 4.736
IPC_protein 4.546
Toseland 4.418
ProMoST 4.647
Dawson 4.482
Bjellqvist 4.635
Wikipedia 4.342
Rodwell 4.393
Grimsley 4.329
Solomon 4.482
Lehninger 4.431
Nozaki 4.596
DTASelect 4.698
Thurlkill 4.418
EMBOSS 4.368
Sillero 4.66
Patrickios 3.846
IPC_peptide 4.495
IPC2_peptide 4.66
IPC2.peptide.svr19 4.591
Protein with the highest isoelectric point:
>tr|A0A4P8W770|A0A4P8W770_9VIRU Uncharacterized protein OS=Tortoise microvirus 55 OX=2583160 PE=4 SV=1
MM1 pKa = 7.51 ISSISLQLLIVTRR14 pKa = 11.84 WRR16 pKa = 11.84 MSDD19 pKa = 3.26 MSCSNPLTINVKK31 pKa = 9.67 GRR33 pKa = 11.84 AVSVPCRR40 pKa = 11.84 YY41 pKa = 10.15 CMSCRR46 pKa = 11.84 TEE48 pKa = 4.02 YY49 pKa = 10.73 QSALLFGAKK58 pKa = 9.68 HH59 pKa = 5.83 EE60 pKa = 5.09 LFDD63 pKa = 4.85 CYY65 pKa = 10.42 RR66 pKa = 11.84 AGLGASFVTLTYY78 pKa = 10.87 SDD80 pKa = 3.29 NCLPEE85 pKa = 4.76 NGSLRR90 pKa = 11.84 KK91 pKa = 9.53 KK92 pKa = 10.45 DD93 pKa = 3.61 VQNLLKK99 pKa = 10.51 NVRR102 pKa = 11.84 IQAKK106 pKa = 9.67 RR107 pKa = 11.84 KK108 pKa = 7.62 TNLPPFKK115 pKa = 10.65 YY116 pKa = 10.15 IMCGEE121 pKa = 4.19 YY122 pKa = 10.25 GDD124 pKa = 4.21 KK125 pKa = 10.43 FGRR128 pKa = 11.84 PHH130 pKa = 4.73 YY131 pKa = 9.98 HH132 pKa = 5.82 IVFIGLSDD140 pKa = 3.74 VLVHH144 pKa = 6.66 NFVKK148 pKa = 10.37 PLWKK152 pKa = 10.38 FGLIDD157 pKa = 3.29 IGVLRR162 pKa = 11.84 EE163 pKa = 3.78 GGLRR167 pKa = 11.84 YY168 pKa = 9.55 ALKK171 pKa = 10.43 YY172 pKa = 7.66 CTKK175 pKa = 10.26 AARR178 pKa = 11.84 GKK180 pKa = 10.15 KK181 pKa = 10.14 AEE183 pKa = 4.18 EE184 pKa = 4.68 LYY186 pKa = 11.0 DD187 pKa = 3.64 NQSIEE192 pKa = 4.29 RR193 pKa = 11.84 PFICHH198 pKa = 5.72 SRR200 pKa = 11.84 GIGKK204 pKa = 8.54 NWFMKK209 pKa = 10.65 NADD212 pKa = 4.98 DD213 pKa = 5.48 IIAHH217 pKa = 6.03 NFTYY221 pKa = 10.71 LEE223 pKa = 3.99 NGIRR227 pKa = 11.84 KK228 pKa = 8.03 PVPAYY233 pKa = 9.93 FRR235 pKa = 11.84 RR236 pKa = 11.84 QYY238 pKa = 11.51 DD239 pKa = 3.23 AFKK242 pKa = 10.04 TFDD245 pKa = 3.19 VSEE248 pKa = 4.42 SIKK251 pKa = 10.67 YY252 pKa = 10.17 LSEE255 pKa = 3.53 EE256 pKa = 4.2 ARR258 pKa = 11.84 LHH260 pKa = 6.91 GYY262 pKa = 10.58 DD263 pKa = 5.19 NYY265 pKa = 11.19 RR266 pKa = 11.84 DD267 pKa = 3.24 WSKK270 pKa = 11.22 QKK272 pKa = 9.69 TYY274 pKa = 7.88 QTEE277 pKa = 4.25 KK278 pKa = 10.76 NLTAAARR285 pKa = 11.84 ASGIPVDD292 pKa = 4.72 DD293 pKa = 3.79 SSLRR297 pKa = 11.84 LQSGFTANDD306 pKa = 3.06 YY307 pKa = 10.93 RR308 pKa = 11.84 PLVNTLLAPRR318 pKa = 11.84 RR319 pKa = 11.84 EE320 pKa = 3.94 ATTNFKK326 pKa = 10.31 EE327 pKa = 4.17
Molecular weight: 37.53 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.837
IPC2_protein 8.843
IPC_protein 8.756
Toseland 9.282
ProMoST 9.194
Dawson 9.619
Bjellqvist 9.458
Wikipedia 9.823
Rodwell 9.809
Grimsley 9.706
Solomon 9.663
Lehninger 9.604
Nozaki 9.531
DTASelect 9.37
Thurlkill 9.472
EMBOSS 9.75
Sillero 9.619
Patrickios 4.558
IPC_peptide 9.648
IPC2_peptide 8.287
IPC2.peptide.svr19 7.796
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
8
0
8
1791
76
571
223.9
25.18
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.203 ± 1.202
1.284 ± 0.518
6.253 ± 0.417
4.858 ± 0.657
4.802 ± 0.641
5.36 ± 0.717
1.563 ± 0.184
5.416 ± 0.791
4.69 ± 1.019
9.436 ± 1.006
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.127 ± 0.391
5.918 ± 0.897
4.634 ± 0.608
3.573 ± 1.075
5.695 ± 0.398
9.38 ± 0.741
6.477 ± 0.48
5.025 ± 0.515
0.838 ± 0.22
4.467 ± 0.654
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here