Pseudoalteromonas phage XCL1123
Average proteome isoelectric point is 6.3
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 79 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A649V2B3|A0A649V2B3_9CAUD Uncharacterized protein OS=Pseudoalteromonas phage XCL1123 OX=2655003 PE=4 SV=1
MM1 pKa = 7.49 TLTIEE6 pKa = 4.21 TVKK9 pKa = 10.85 AYY11 pKa = 10.39 GGTGNDD17 pKa = 2.72 IVIQAKK23 pKa = 9.45 IDD25 pKa = 3.6 AFATIYY31 pKa = 10.05 TCLTSSYY38 pKa = 10.78 NSALADD44 pKa = 5.1 DD45 pKa = 4.61 IANSYY50 pKa = 9.55 IAGTLQSVSGEE61 pKa = 4.18 GQVTSRR67 pKa = 11.84 KK68 pKa = 9.69 APNGASRR75 pKa = 11.84 NFKK78 pKa = 8.81 QNKK81 pKa = 8.42 YY82 pKa = 9.64 GDD84 pKa = 3.49 SGEE87 pKa = 4.15 YY88 pKa = 10.73 DD89 pKa = 3.09 NGLLADD95 pKa = 4.71 AYY97 pKa = 10.67 RR98 pKa = 11.84 WDD100 pKa = 4.03 TNFCLPIEE108 pKa = 4.09 PSYY111 pKa = 10.99 FVGTAGTVYY120 pKa = 10.35 PADD123 pKa = 3.76 NPQQ126 pKa = 2.74
Molecular weight: 13.47 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.139
IPC2_protein 4.38
IPC_protein 4.266
Toseland 4.05
ProMoST 4.418
Dawson 4.24
Bjellqvist 4.406
Wikipedia 4.19
Rodwell 4.088
Grimsley 3.961
Solomon 4.24
Lehninger 4.19
Nozaki 4.368
DTASelect 4.609
Thurlkill 4.113
EMBOSS 4.202
Sillero 4.38
Patrickios 0.655
IPC_peptide 4.228
IPC2_peptide 4.355
IPC2.peptide.svr19 4.271
Protein with the highest isoelectric point:
>tr|A0A649V0I2|A0A649V0I2_9CAUD Uncharacterized protein OS=Pseudoalteromonas phage XCL1123 OX=2655003 PE=4 SV=1
MM1 pKa = 6.87 KK2 pKa = 10.14 QCRR5 pKa = 11.84 TRR7 pKa = 11.84 SNAHH11 pKa = 5.92 FNGILTMQMMGWTKK25 pKa = 10.84 GYY27 pKa = 8.35 TLDD30 pKa = 3.99 LPVWRR35 pKa = 11.84 VNKK38 pKa = 10.12 VKK40 pKa = 10.81 FNVHH44 pKa = 5.35 GVV46 pKa = 3.23
Molecular weight: 5.4 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.3
IPC2_protein 9.809
IPC_protein 10.277
Toseland 10.847
ProMoST 10.409
Dawson 10.921
Bjellqvist 10.555
Wikipedia 11.067
Rodwell 11.389
Grimsley 10.95
Solomon 11.008
Lehninger 10.994
Nozaki 10.833
DTASelect 10.555
Thurlkill 10.833
EMBOSS 11.228
Sillero 10.847
Patrickios 11.199
IPC_peptide 11.023
IPC2_peptide 9.472
IPC2.peptide.svr19 8.525
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
79
0
79
14046
41
826
177.8
19.82
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.967 ± 0.366
1.317 ± 0.185
6.258 ± 0.199
6.6 ± 0.265
3.887 ± 0.174
7.233 ± 0.35
1.424 ± 0.138
6.244 ± 0.19
6.998 ± 0.379
7.511 ± 0.204
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.663 ± 0.169
6.059 ± 0.226
3.218 ± 0.163
4.058 ± 0.222
3.93 ± 0.184
7.048 ± 0.345
5.845 ± 0.301
6.429 ± 0.228
1.346 ± 0.13
3.966 ± 0.197
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here