Slackia exigua (strain ATCC 700122 / DSM 15923 / CIP 105133 / JCM 11022 / KCTC 5966 / S-7)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Coriobacteriia; Eggerthellales; Eggerthellaceae; Slackia; Slackia exigua

Average proteome isoelectric point is 6.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2027 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D0WHA1|D0WHA1_SLAES Transcriptional regulator TetR family OS=Slackia exigua (strain ATCC 700122 / DSM 15923 / CIP 105133 / JCM 11022 / KCTC 5966 / S-7) OX=649764 GN=HMPREF0762_01133 PE=4 SV=1
MM1 pKa = 7.6PLKK4 pKa = 8.78EE5 pKa = 4.25TQMTNPTARR14 pKa = 11.84AMSRR18 pKa = 11.84AEE20 pKa = 4.17FLALMAGTAVPAALALSGCSLPAPSDD46 pKa = 3.72LPSGDD51 pKa = 2.92GRR53 pKa = 11.84GIFSPEE59 pKa = 3.8PAPVEE64 pKa = 3.69PLAPVRR70 pKa = 11.84DD71 pKa = 3.82EE72 pKa = 4.1VDD74 pKa = 2.87IPGALEE80 pKa = 4.58DD81 pKa = 4.04VPSSYY86 pKa = 10.82FDD88 pKa = 3.87PSNEE92 pKa = 3.91PGTLEE97 pKa = 4.01RR98 pKa = 11.84FYY100 pKa = 11.29YY101 pKa = 9.18DD102 pKa = 2.89TNTYY106 pKa = 10.88DD107 pKa = 4.3DD108 pKa = 4.18EE109 pKa = 5.91ARR111 pKa = 11.84DD112 pKa = 3.75MEE114 pKa = 4.6KK115 pKa = 10.44FAIVYY120 pKa = 9.44LPYY123 pKa = 10.65GYY125 pKa = 10.77DD126 pKa = 3.42PDD128 pKa = 4.86DD129 pKa = 4.33AEE131 pKa = 3.91TRR133 pKa = 11.84YY134 pKa = 10.33NVLYY138 pKa = 10.57LMHH141 pKa = 6.93GASGSEE147 pKa = 3.64SSYY150 pKa = 10.86FGTPEE155 pKa = 3.74EE156 pKa = 4.42PYY158 pKa = 10.98GDD160 pKa = 4.24KK161 pKa = 10.84FLLDD165 pKa = 3.57SMIAQGDD172 pKa = 4.46MNPMIVVCVSYY183 pKa = 11.15YY184 pKa = 9.81PDD186 pKa = 5.35NIEE189 pKa = 5.07QDD191 pKa = 3.3NDD193 pKa = 3.78DD194 pKa = 4.05YY195 pKa = 12.0DD196 pKa = 3.73AALTKK201 pKa = 10.56RR202 pKa = 11.84FGYY205 pKa = 8.67EE206 pKa = 3.59LRR208 pKa = 11.84DD209 pKa = 3.4LVPQFEE215 pKa = 4.67TRR217 pKa = 11.84YY218 pKa = 8.2NTYY221 pKa = 10.62LEE223 pKa = 4.49SSDD226 pKa = 5.58DD227 pKa = 3.77EE228 pKa = 5.78DD229 pKa = 3.68IVASRR234 pKa = 11.84GHH236 pKa = 6.84RR237 pKa = 11.84IFGGFSMGAVTTWYY251 pKa = 10.86RR252 pKa = 11.84MTDD255 pKa = 2.96SMDD258 pKa = 2.5WFEE261 pKa = 4.97YY262 pKa = 9.05FFPMSGSLFWSSEE275 pKa = 3.81MYY277 pKa = 9.47TEE279 pKa = 4.19RR280 pKa = 11.84RR281 pKa = 11.84SAEE284 pKa = 3.55WTADD288 pKa = 3.6FLVDD292 pKa = 4.97SIDD295 pKa = 3.67DD296 pKa = 3.49QGYY299 pKa = 8.8GLDD302 pKa = 3.87DD303 pKa = 4.04FFVFTTTGSLDD314 pKa = 3.46FALGGLDD321 pKa = 3.53LQVGKK326 pKa = 10.26LRR328 pKa = 11.84KK329 pKa = 9.73HH330 pKa = 6.3PDD332 pKa = 2.99FFVFDD337 pKa = 4.84DD338 pKa = 4.14DD339 pKa = 4.98AEE341 pKa = 4.32NATRR345 pKa = 11.84VAPNAAYY352 pKa = 9.6RR353 pKa = 11.84VAAGYY358 pKa = 10.21SHH360 pKa = 7.68DD361 pKa = 3.69SDD363 pKa = 5.08GRR365 pKa = 11.84DD366 pKa = 3.1TYY368 pKa = 10.86IYY370 pKa = 9.85NCLPIFSEE378 pKa = 4.91LMGG381 pKa = 4.47

Molecular weight: 42.67 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D0WHI0|D0WHI0_SLAES 5-formyltetrahydrofolate cyclo-ligase OS=Slackia exigua (strain ATCC 700122 / DSM 15923 / CIP 105133 / JCM 11022 / KCTC 5966 / S-7) OX=649764 GN=HMPREF0762_01218 PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.42RR3 pKa = 11.84TYY5 pKa = 10.31QPNKK9 pKa = 7.52RR10 pKa = 11.84HH11 pKa = 5.83RR12 pKa = 11.84AKK14 pKa = 9.65THH16 pKa = 5.05GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.11GGRR28 pKa = 11.84AVLSARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.06GRR39 pKa = 11.84KK40 pKa = 8.94KK41 pKa = 10.21LTVV44 pKa = 3.1

Molecular weight: 5.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2027

0

2027

632442

35

2350

312.0

33.83

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.087 ± 0.079

1.55 ± 0.027

6.434 ± 0.052

6.147 ± 0.059

3.868 ± 0.04

8.41 ± 0.05

1.936 ± 0.019

5.355 ± 0.049

3.637 ± 0.045

8.722 ± 0.06

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.853 ± 0.026

2.586 ± 0.029

4.246 ± 0.038

2.495 ± 0.029

6.453 ± 0.077

6.413 ± 0.048

5.156 ± 0.05

7.872 ± 0.051

1.037 ± 0.022

2.742 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski