Byssochlamys spectabilis (strain No. 5 / NBRC 109023) (Paecilomyces variotii)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; Eurotiomycetes; Eurotiomycetidae; Eurotiales; Thermoascaceae; Paecilomyces; Paecilomyces variotii

Average proteome isoelectric point is 6.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8877 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|V5HZC9|V5HZC9_BYSSN VOC domain-containing protein OS=Byssochlamys spectabilis (strain No. 5 / NBRC 109023) OX=1356009 GN=PVAR5_4012 PE=4 SV=1
MM1 pKa = 7.2SVKK4 pKa = 9.92TFITSSLAIIGLASAQSSVISAFIPDD30 pKa = 4.46TDD32 pKa = 3.99PQPLVASIMGNDD44 pKa = 3.69AAATTYY50 pKa = 10.88FLTCPAGTDD59 pKa = 3.82GSDD62 pKa = 3.13CGMGPGMTLISGPKK76 pKa = 6.74TAGWMVQVPEE86 pKa = 4.07EE87 pKa = 4.17DD88 pKa = 3.57LYY90 pKa = 11.83GSIRR94 pKa = 11.84CSMGGTTTAICTSSMGGSGANFPGVSTEE122 pKa = 4.1TLDD125 pKa = 4.49ASDD128 pKa = 4.0ISLLPVTVTAGSTTPVAASASATVAASGSGATSAAPTGASQASSPEE174 pKa = 3.82PSASRR179 pKa = 11.84TSSGSSVSASSTKK192 pKa = 10.47EE193 pKa = 3.88SGTSTSATSTGAAASLLSADD213 pKa = 3.93AQMVMGLAAVAAGVVAVMM231 pKa = 5.02

Molecular weight: 22.04 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|V5G700|V5G700_BYSSN Ankyrin repeat protein OS=Byssochlamys spectabilis (strain No. 5 / NBRC 109023) OX=1356009 GN=PVAR5_5308 PE=4 SV=1
MM1 pKa = 7.53LCLRR5 pKa = 11.84CSRR8 pKa = 11.84ALPSAFNASSRR19 pKa = 11.84VTAQSQILRR28 pKa = 11.84FASRR32 pKa = 11.84TPSARR37 pKa = 11.84TFSSLLNSQRR47 pKa = 11.84PALSIHH53 pKa = 6.65RR54 pKa = 11.84AHH56 pKa = 6.67SPIISSLATNSANATAAPSAPLALTSQSRR85 pKa = 11.84SFSASASLAGKK96 pKa = 9.72RR97 pKa = 11.84DD98 pKa = 3.34TYY100 pKa = 10.99NPSRR104 pKa = 11.84RR105 pKa = 11.84VQKK108 pKa = 10.2RR109 pKa = 11.84RR110 pKa = 11.84HH111 pKa = 5.33GFLARR116 pKa = 11.84LRR118 pKa = 11.84SRR120 pKa = 11.84GGRR123 pKa = 11.84KK124 pKa = 8.7ILLRR128 pKa = 11.84RR129 pKa = 11.84RR130 pKa = 11.84AKK132 pKa = 10.05GRR134 pKa = 11.84KK135 pKa = 7.93FLSWW139 pKa = 3.26

Molecular weight: 15.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8877

0

8877

4775538

42

5370

538.0

59.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.432 ± 0.022

1.141 ± 0.008

5.728 ± 0.018

6.342 ± 0.025

3.735 ± 0.014

6.83 ± 0.024

2.335 ± 0.01

5.019 ± 0.017

4.852 ± 0.024

8.899 ± 0.026

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.084 ± 0.008

3.656 ± 0.014

6.067 ± 0.025

3.967 ± 0.018

6.199 ± 0.023

8.503 ± 0.03

5.823 ± 0.016

6.132 ± 0.018

1.425 ± 0.008

2.831 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski